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Cloning and expression of odorant receptor gene PlxyOr83b from Plutella xyostella (Lepidoptera: Plutellidae) PDF

2011·4.2 MB·
by  XunLi
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Preview Cloning and expression of odorant receptor gene PlxyOr83b from Plutella xyostella (Lepidoptera: Plutellidae)

ASA RS RE A PlxyOr83b AA Fee BIA Ala al Cea PEMAMTE. | A, iF WHE, LWA Hts eee REI OS AT AUTRE, ATA DEE SS Fila, FELAUSTVARL JOM [AGERE GOA. ERED and, BALE Se SE, ARTA SRM ARCUS. PN LMP Alec ot RSA asta CHIR ACH RAPS AMAT LA Cae Hk, AL RE BH SUSUMU Rane MEI fe CDGcF1 AQT 7a SB ANA ea, ALP RRS ACLS Lo. AY RIL, 29 $F POD Lea 2 HEL OAT EDO) RAAT is APO GS RS, Ue TRUSSO UMD, Wate ESR AIA Hot KE we BALMS. [PETA RNR T AIS Esa olesS. alee BEG CD Pha, De ee ee WRRRE, Yeo Rta: Aa, aotane iu Cloning and expression of odorant receptor gene PhxyOr83b fom Pltelia ayostela (Lepidoptera; Platellidac} LL Sua, LIU Jingle. GUNG Liang, CIN Yoo, ZHONG Gecdiua Sh Cbs Asien Thibe-sig, Cnglnn SIRGSE, C3 Abeiret; [Je] «LIN cloning aad prokaseic squcsion of Psat cla otc "le pi te His far aadhing se reali char af election elaine [otetede} eb Labvony a nee Inv socepar Usb, see ane! omwoun # syle and host plate. and developing iacet hesarioe rpulaas cervaling OB Peptic waaay! 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HMMA do LS) Western blot $34 Weatem Mot 20RD L116 lie BOTEC AUIEC An Hsantibody), SHH ARETE SP HRP SITE Cont an Moe 2, PB ARE so fTANRET A (NC) THUR eC Se aE WHHL 1 PLS, 25 Ivoen20) AHL 2 h, Ll DRST (#288 Treen 20 If x PRS) Shak =? Be te RET ES HAR & 5% BSA HH Loc, 29% Yecor-20) MASONS, JC RARE REAL), POST BERR 3 HK, AK 10 IAM ARE. hh, PBST 9E3 Ye, APICW enny ‘WACO eu HI 22g 809 DAL APARALET NC BY EM. IRICEN. 2 BRSaH 2A PlyOnkat AVENE IALSHE HE Ona PRAM Sa HR HAIRS, HAE Goubank File O83 AAMT MARIE EIB. PENSEME & eDNS BUDE AT POR ae, ae 1400 WAAL), SEAR ARES OM Wer AEA, HE De GEIR, ELSES WML. NS BFR PL). RAB Aaa 3b USEABLE 1413 bp, ESAT. RUSE HAUS {2h 7.19, Signal P SA AEM AEA Si, AROUTSHIL. Ae Wat SC See MTIR NBR 7 CooRonk ELAS ALE Lt O83 HEATH SRM ATMIE, KIS RETA, MTROOR, AC YER Haioverpa anata HAW Leactege sss SRA Ok HIELILPH [80% , FEACTEAL O88 SSeS RAEI AERC 9. SLO BRINN, O6db fH 49 PeyOr83_ AF ConRank PRESET GOQNRBNIN, ARH HMOTTOP AE 03H) PlyOss RIEL, SEAM EAM AR LINE 7PM. LN SEAT, AIL seMk OSL ANC Play DNA 0} POR srg {e188 of Os i pele (Hyd) vse ee ek na oh PER pest 2.2 PACRSSMG EAL PCR ee RR ERY PET -PieyOr83b (PCR RSL REEL, SHUN YS Wa, IPE 1 AO Be GU sk. RS, MUTE, i PeyOS8b CAEAMIEA pET-S2a 11. 2.3 PleyOrSb FIREEAIRPHIRE A RSTO SAMUS RENE TAY Ae EE AT-M eID ACKAGHPH BIZU(DES) , £6 1.0 ammel/L WIG 28°C SeHWSSBIRTE 2. 4. 6 AUS bh, MYFTEGARTE LAVA] SSERREN THERE SUS.PACE WASP, SB ACOH. Te SCHSEHEEF. RCD ase SREP ARMIN 32.0 kD ARASH, 7) AGES RUAN Tae, RA DSR Pye AL de, lw N Oa HE LEW R HR RIESE. UE RANMA), MARELUT PEE FESR 5, BRIER AT HERLZEN'LHLOUINR, EW) pET- MeyOrRdh TERRE IEEE Y 20T, .0 me IPTG 4 Wesel SE LAT 5 ALL, pET-PyO83b 4232.0 kD ALAR A SAESAPCS), SYGISHIRREM Poy On83b (ir ADRS SASL CDS, SOIT FLARES, a + UPC A IGS dwar Pei ec wl adie SS FS BNO RA TOTAL rote: $s ASIEN Sita Poe, angers ANE Batya I apes haa {es Bt ints ann CR vn eras FT ARM avs Us RAT Robe Pees AAP eB bis, $B One math RAS sve ta nt ta S Sembee Sinn go Ane A sh ies ny 8 penn ee IRAE sate cg pnts; MDa tis oe EY eres MAMI nance nna iin, SOT ge in A nt AANA KH cs en su Ae Ace Eatin Sn Be Fs UGA pe reo HI SEAR hte i DN NE Mer EH, IRAENIOR ped ET MIS RAGHU DETR CaN 1ig.5 Mersin cadence dino Tips DVS FRA DU Mes DE, LPI, FRR 2 4,6. 8 3A sinh i My ESLER A Rar leo AT TIF pit Hheyoesh SPIER Svs Pace: ki 1g? 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