CLONALITY STUDIES IN MYELODYSPLASIA AND ACUTE MYELOID LEUKEMIA Page 2 Page 3 ... PDF
Preview CLONALITY STUDIES IN MYELODYSPLASIA AND ACUTE MYELOID LEUKEMIA Page 2 Page 3 ...
CLONALITY STUDIES IN MYELODYSPLASIA AND ACUTE MYELOID LEUKEMIA Clonality Studies in Myelodysplasia aud Acute Myeloid Leukemia Klonaliteitsstudies in myelodysplasie en acute myelolde leukemie Proefschrift ter verkrijging van de graad van doctor aan de Erasmus Universiteit Rotterdam op gezag van de rector magnificus Prof. dr. ir. J. H. van Bemmel en volgens besluit van het College voor Promoties De openbare verdediging zal plaatsvinden op woensdag 27 september 2000 am 11.45 uur door Kirsten van Lorn geboren te Rotterdam Promotiecommissie Promotoren: Prof. dr. B. Lowenberg Prof. dr. A. Hagemeijer Overige leden: Prof. dr. 1. 1. M. van Dongen Prof. dr. A. Hagenbeek Prof. dr. I. Touw CONTENTS Abbreviations 9 Chapter I Introduction 1.1 Hematopoiesis and malignant transformation 13 1.2 Myelodysplastic syndrome 13 1.2.2 Pathogenesis and biological features 16 1.3 Acute myeloid leukemia 17 1.3.2 Pathogenesis and biological features 20 1.4 De novo and secondary MDS and AML 20 1.5 Chromosome analysis: cytogenetic and molecular methods 20 Conventional chromosome analysis 21 In situ hybridization 21 Southern blotting 24 Reverse transcriptase-polymerase chain reaction 24 1.6 Chromosome abnormalities and FAB subtypes 25 1.7 Clonality assays 27 1.8 Introduction to the experimental work and aims of this study 29 1.9 References 30 Chapter 2 In situ hybridization on May-Griinwald-Giemsa stained 47 Bone marrow and blood smears of patients with hematological disorders allows detection of cc111ineage specific cytogenetic abnormalities Blood 82: 884-888, 1993 Chapter 3 Cytogenetic c10nality analysis in myelodysplastic syndrome: 61 monosorny 7 can be demonstrated in the myeloid and in the lymphoid lineage Leukemia 9: 1818-1821, 1995 Chapter 4 Cytogenetic clonality analysis: typical patterns in 73 myelodysplastic syndrome and acute myeloid leukaemia Br J Haematol 93: 594-600, 1996 Chapter 5 Clonality analysis of hematopoietic ceIl lineages in acute 87 myeloid leukemia and translocation (8;21): only myeloid cells are part of the malignant clone Leukemia 11: 202-205, 1997 Chapter 6 Cytogenetic c10nality analysis of megakaryocytes in 97 rnyelodyspJastic syndrome by dual-color fluorescence in situ hybtidization and confocal laser scanning microscopy Genes, Chromosomes and Cancer 25: 332-338, 1999 Chapter 7 General discussion and summary 111 Samenvatting 121 List of publications 124 CUlTiculum Vitae 126 Dankwoord 127 List of frequently used abbreviations AML acute myeloid leukemia AMLl acute myeloid leukemia-l gene BM bone marrow CD cluster of differentiation! cluster of designation CLSM confocal laser scanning microscope CMML chronic myclomonocytic leukemia CR complete remission DNA deoxytibonuc1eic acid ETG eight-twenty-one gene FAB Frcnch-American-British classification of MDS and AML FISH fluorescence in situ hybridization FITC fluorescein isothiocyanate ISH in situ hybridization JMML juvenile myelomonocytic leukemia LT-MDS leukemia transformed MDS MDS myelodysplastic syndrome MGG May-Grunwald Giemsa MoAb monoclonal antibody PB peripheral blood PCR polymerase chain reaction RA refractory anemia RAEB RA with excess of blasts RAEB-t RAEB in transformation RARS RA with ringed sideroblasts RT-PCR reverse transcliptase peR SSC standard saline citrate: 150 rnM NaCI + 15rnM sodium citrate 9
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