Circulating Nucleic Acids in Plasma and Serum: Proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held on 9-11 November 2009 in Hong Kong.

306 Pages·2011·7.56 MB·English

by  Peter B. Gahan (auth.)

Circulating Nucleic Acids in Plasma and Serum

Peter B. Gahan
Editor
Circulating Nucleic Acids
in Plasma and Serum
Proceedings of the 6th International
Conference on Circulating Nucleic Acids
in Plasma and Serum Held on 9–11
November 2009 in Hong Kong
1 3
Editor
Prof.PeterB.Gahan
King’sCollegeLondon
Anatomy&HumanSciences
LondonBridge
SE11ULLondon
UnitedKingdom
[email protected]
ISBN978-90-481-9381-3 e-ISBN978-90-481-9382-0
DOI10.1007/978-90-481-9382-0
SpringerDordrechtHeidelbergLondonNewYork
LibraryofCongressControlNumber:2010933647
©SpringerScience+BusinessMediaB.V.2011
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Contents
PartI CurrentDevelopments
1 Current Developments in Circulating Nucleic Acids
inPlasmaandSerum . . . . . . . . . . . . . . . . . . . . . . . . . . 3
PeterB.Gahan
PartII NucleicAcidsinOncology–DiagnosisandPrognosis
andMetastases
2 ReflectionsonaLifeofCNAPS:FromCirculatingDNA
totheVirtosome . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
MauriceStroun
3 CirculatingTumor-RelatedDNAAlterationsasProstate
CancerBiomarkers . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
LaurentLessard,EijiSumami,andDaveS.B.Hoon
4 ParallelTagged454SequencingfortheCharacterization
ofCirculatingDNA . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
ManieshvanderVaart,DmitryV.Semenov,ElenaV.Kuligina,
VladimirA.Richter,andPietJ.Pretorius
5 AdvancedAnalysisofHumanPlasmaCirculatingDNA
Sequences Produced by Parallel Tagged Sequencing
onthe454Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
ManieshvanderVaart,DmitryV.Semenov,ElenaV.Kuligina,
VladimirA.Richter,andPietJ.Pretorius
6 ConcentrationandDistributionofSingle-Copyβ-Actin
GeneandLINE-1RepetitiveElementsinBloodofLung
CancerPatients . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
AnastasiaA.Ponomaryova,ElenaY.Rykova,
NadezhdaV.Cherdyntseva,TatianaE.Skvortsova,
AnnaV.Cherepanova,EvgeniyS.Morozkin,
VladislavA.Mileiko,NikolaiV.Litvjakov,
AlexeyY.Dobrodeev,AlexanderA.Zav’yalov,
SergeyA.Tuzikov,ElenaD.Chikova,ValentinV.Vlassov,
andPavelP.Laktionov
v
vi Contents
7 Plasma DNA Methylation Analysis in Predicting
Short-TermRecurrenceofSurgicalPatientswithNon-small
CellLungCancer(NSCLC) . . . . . . . . . . . . . . . . . . . . . . 47
QingqingDing,YuanMu,ShiyangPan,YongqianShu,
ShijiangZhang,BingfengZhang,HongWang,LiGao,
WenyingXia,JianXu,MeijuanZhang,YuanyuanZhang,
YanCao,andShanLu
8 BloodBasedMethylatedDNAandTumor-SpecificProtein
AnalysisinGastricCancerDiagnostics . . . . . . . . . . . . . . . . 57
ElenaV.Elistratova,PetrI.Shelestyuk,
ValentinaI.Permyakova,ElenaD.Chikova,
SergeyA.Tuzikov,ValentinV.Vlassov,PavelP.Laktionov,
andElenaY.Rykova
9 Increase in Circulating MicroRNA Levels in Blood
ofOvarianCancerPatients . . . . . . . . . . . . . . . . . . . . . . 63
CarinaRoth,SabineKasimir-Bauer,MartinHeubner,
KlausPantel,andHeidiSchwarzenbach
10 TheCourseofCirculatingNucleosomesinLiverCancer
PatientsUndergoingTransarterialChemoembolizationTherapy . 73
NikolausKohles,DorotheaNagel,DietrichJüngst,
JürgenDurner,PetraStieber,andStefanHoldenrieder
11 Presence of Nucleosomes in Cerebrospinal Fluid
ofGlioblastomaPatients–PotentialforTherapyMonitoring . . . 79
StefanHoldenrieder,AndreasSpuler,MichaelTischinger,
DorotheaNagel,andPetraStieber
12 CirculatingNucleosomesandDNAseinBreastCancer
PatientsDuringNeoadjuvantChemotherapy . . . . . . . . . . . . 85
OliverJ.Stoetzer,DeboraM.I.Fersching,and
StefanHoldenrieder
13 CirculatingNucleosomesinCancerPatientswithLiver
Metastases Undergoing Selective Internal Radiation
TherapyUsingYttrium-90LabelledMicrospheres . . . . . . . . . 91
YvonneFehr,StefanHoldenrieder,Ralf-ThorstenHoffmann,
KlausTatsch,TobiasJakobs,DorotheaNagel,andPetraStieber
14 H3K9me3/H4K20me3RatioinCirculatingNucleosomes
asPotentialBiomarkerforColorectalCancer . . . . . . . . . . . . 97
UgurDeligezer,ElifZ.Akisik,EbruE.Akisik,
MügeKovancilar,DursunBugra,NilgünErten,
StefanHoldenrieder,andNejatDalay
Contents vii
15 FunctionalityofCNAPSinCancer:TheTheoryofGenometastasis 105
DoloresC.García-Olmo,HectorGuadalajara,
CarolinaDominguez-Berzosa,MaríaG.Picazo,
MarianoGarcía-Arranz,andDamiánGarcía-Olmo
PartIII NucleicAcidsinFoetalMedicine
16 Circulating Fetal DNA/RNA in Maternal Plasma
forAneuploidyDetection . . . . . . . . . . . . . . . . . . . . . . . 111
Y.K.Tong,R.W.K.Chiu,andY.M.D.Lo
17 A “Fluid-Agnostic” Approach to Analysis of Fetal
andNeonatalDevelopmentalGeneExpression. . . . . . . . . . . . 125
JillL.MaronandDianaW.Bianchi
18 Non-invasivePrenatalDiagnosis:AnEpigeneticApproach
totheDetectionofCommonFetalChromosomeDisorders
byAnalysisofMaternalBloodSamples . . . . . . . . . . . . . . . 133
MajA.Hultén,ElisavetA.Papageorgiou,FlorianaDella
Ragione,MaurizioD’Esposito,NigelCarter,and
PhilipposC.Patsalis
19 ComparativeStudyofExtracellularDNAbyFISH . . . . . . . . . 143
EvgeniyS.Morozkin,EkaterinaM.Loseva,
VladislavA.Mileiko,KiraS.Zadesenets,NikolayB.Rubtsov,
ValentinV.Vlassov,andPavelP.Laktionov
20 An Additional Pre-amplification Step for the Early
DeterminationofFetalRHDfromMaternalPlasma . . . . . . . . 147
TadejaDovcˇ-Drnovšek,NatašaToplak,IrenaBricl,
TanjaBlejec,MinkaKovacˇ,andPrimožRožman
21 TheCorrelationofCirculatingCell-FreeDNA,Cell-Free
Fetal DNA and MicroRNA 325 Levels to Clinical
CharacteristicsandLaboratoryParametersinPre-eclampsia . . . 153
LeventeLázár,BálintNagy,AttilaMorvarec,andJánosRigó
PartIV OtherClinicalExploitationofCNAPS
22 ComparisonofPlasmaCell-FreeDNALevelswithGene
Expression Profiles of Peripheral Blood Cells During
Haemodialysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
AlesHorinek,AlesPanczak,MagdalenaMokrejsova,
KatarinaRocinova,MarieKorabecna,DaliborCerny,and
VladimirTesar
23 Low-Molecular-Weight DNA of Blood Plasma
asanIndicatorofPathologicalProcesses . . . . . . . . . . . . . . . 165
IrinaN.Vasilyeva,TatyanaV.Ivtchik,andIgorA.Voznyuk
viii Contents
24 TheClinicalSignificanceofPlasmaDNAQuantification
forQuakeTraumaPatients . . . . . . . . . . . . . . . . . . . . . . 171
DanChen,ShiyangPan,ShijiangZhang,PeijunHuang,
WenyingXia,ErfuXie,BingGu,FangWang,JianXu,
TingXu,YachunLu,DiYang,andShanLu
PartV TheBiologyofCNAPS
25 MethylatedCell-FreeDNAInVitroandInVivo . . . . . . . . . . . 185
TatyanaE.Skvortsova,OlgaE.Bryzgunova,
AlenaO.Lebedeva,ViktoriaV.Mak,ValentinV.Vlassov,and
PavelP.Laktionov
26 CircadianRhythmicityandClearanceofCell-FreeDNA
inHumanPlasma . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
MarieKorabecna,AlesHorinek,NikolaBila,and
SylvieOpatrna
27 Fragments of Cell-Free DNA (cfDNA) Enhance
TranscriptionActivityinHumanMesenchymalStemCells
(hMSCs)andInhibitTheirInVitroDifferentiation . . . . . . . . . 199
ElenaM.Malinovskaya,SvetlanaV.Kostyuk,
AlekseyV.Ermakov,MarinaS.Konkova,
TatjanaD.Smirnova,LarisaV.Kameneva,
LiudmilaV.Efremova,AnnaYu.Alekseeva,
LiudmilaN.Lyubchenko,andNatalyaN.Veiko
28 Cell-Surface-BoundDNAInhibitsPoly(I:C)-ActivatedIL-6
andIL-8ProductioninHumanPrimaryEndothelialCells
andFibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207
AnnaV.Cherepanova,AlexanderV.Bushuev,
ValentinV.Vlassov,andPavelP.Laktionov
29 AccumulatingFragmentsofExtracellularDNA(ecDNA)
InfluenceRatPrimaryCerebellumGranuleCellCulture . . . . . . 213
LiudmilaV.Efremova,SvetlanaV.Kostyuk,
LeonidG.Khaspekov,andNatalyaN.Veiko
30 CellFreeDNA(cfDNA)InfluencesNitricOxideandros
LevelsinHumanEndothelialCells . . . . . . . . . . . . . . . . . . 219
AnnaYu.Alekseeva,NataliaV.Bulycheva,
SvetlanaV.Kostyuk,TatjanaD.Smirnova,and
NatalyaN.Veiko
31 Development oftheAdaptiveResponseandBystander
EffectInducedbyLow-DoseIonisingRadiationinHuman
MesenchymalStemCells . . . . . . . . . . . . . . . . . . . . . . . . 225
AlekseyV.Ermakov,MarinaS.Konkova,SvetlanaV.Kostyuk,
TatjanaD.Smirnova,LiudmilaV.Efremova,
LiudmilaN.Lyubchenko,andNatalyaN.Veiko
Contents ix
32 ExtracellularRNAasRegulatorsofCellularProcesses . . . . . . . 233
DmitryV.Semenov,GrigoryA.Stepanov,
DmitryN.Baryakin,OlgaA.Koval,ElenaV.Kuligina,and
VladimirA.Richter
33 MicrovesiclesCirculatinginPlasmaofRatsContainDNA:
AreTheseSmallVesiclesaMainSourceofCell-FreeDNA
inPlasma? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239
GemmaSerrano-Heras,DamiánGarcía-Olmo,and
DoloresC.García-Olmo
PartVI NewTechnologiesforCNAPS
34 RapidIsolationandDetectionofCellFreeCirculating
DNAandOtherDiseaseBiomarkersDirectlyfromWholeBlood . 247
RajaramKrishnanandMichaelJ.Heller
35 YieldsofViralandCirculatingCell-FreeNucleicAcids
(cid:2)
UsingtheQIAampR CirculatingNucleicAcidKit . . . . . . . . . 259
MartinHorlitz,TanjaHartinger,SimoneGraf,
AnnabelleLucas,AnnetteNocon,and
MarkusSprenger-Haussels
36 ComparisonofNucleosomesandQuantitativePCRUsing
DiverseDNAIsolationMethods . . . . . . . . . . . . . . . . . . . . 269
MichaelFleischhacker,BerndSchmidt,SabineWeickmann,
DeboraM.I.Fersching,GloriaS.Leszinski,BarbaraSiegele,
OliverJ.Stoetzer,andStefanHoldenrieder
37 MicroRNA Analysis in the Spinal Fluid of Alzheimer
Patients:AMethodologicalFeasibilityStudy . . . . . . . . . . . . 275
ArgondevanHarten,JoyceMulders,CaglaÇevik,
MaartjeKester,PhilipScheltens,WiesjevanderFlier,and
CeesOudejans
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283