Ciba Foundation Symposium 13 - Haemopoietic Stem Cells

344 Pages·1973·8.045 MB·English

Eaemopoietic
Stem Cells
J. M. YOFFEY Photograph: Mrs E. Sulomon
The Ciba Fuurrdaiion for the promotion of internationai cooperation in
medical and chemical research is a scientific and educational charity established by
CIBA Limited - now CIBA-GEIG Y Limited- of Basle. The Foundation operates
independently in London under English trust law.
Ciba Foundation Symposia are published in collaboration with
Associated Scientific Publishers (Elsevier Scientific Publishing Company, Excerpta Medica.
North-Holland Publishing Company) in Amsterdam.
Associated Scientific Publishers, P.O. Box 1270, Amsterdam
Haemopoietic
Stem Cells
Ciba Foundation Symposium 13 (new series)
Held in tribute to J. M. Yoffey
1973
-
Elsevier Excerpta Medica North-Holland
*
. . .
Associated Scientific Publishers Amsterdam London New York
(0 Copj.right 1973 Cihn Foundation
All rights reserved. No part of this publication may be reproduced, stored in a retrieval
system or transmitted, in any form or by any means, electronic, mechanical, photocopying,
recording, or otherwise, without permission in writing from the publishers.
ISBN Excerpta Medica 90 219 4014 0
l.SBN American Elsevier 0-444-1 5009-9
Library of Congress Catalog Card Number 73-76975
Published in 1973 by Associated Scientific Publishers, P.O. Box 1270, Amsterdam, and
52 Vanderbilt Avenue, New York, N.Y. 10017.
Suggested series entry for library catalogues: Ciba Foundation Symposia.
Ciba Foundation Symposium 13 (new series)
Printed in The Netherlands by Mouton & Co., The Hague
Contents
J. F. LOUTIT Chairman’s introduction 1
J. M. YOFFEY Stem cell role of the lymphocyte-transitional cell (LT) compart-
ment 5
Discussion 39
K. A. DICRE, M. J. VAN NOORD, B. MAAT, U. W. SCHAEFER & D. W. VAN BEKKUM
Attempts at morphological identification of the haemopoietic stem cell in
primates and rodents 47
Discussion 66
D. B. THOMAS The radiation chimera as an experimental model for the study
of haemopoietic stem cell populations 71
Discussion 99
c. ROSSE Precursor cells to erythroblasts and to small lymphocytes of the bone
marrow 105
Discussion 126
D. G. OSMOND, s. c. MILLER & Y. YOSHIDA Kinetic and haemopoietic properties
of lymphoid cells in the bone marrow 131
Discussion 152
D. METCALF & M. A. s. MOORE Regulation of growth and differentiation in
haemopoietic colonies growing in agar 157
Discussion 175
VIll CONTENTS
E. A. MCCULLOCH, c. J. GREGORY & J. E. TILL Cellular communication early in
haemopoietic differentiation 183
Discussion 199
F. STOHLMAN, Jr., P. QUESENBERRY, E. NISKANEN, A. MORLEY, W. TYLER, K. RICKARD,
M. SYMANN, F. MONETTE & D. HOWARD Control of granulopoiesis 205
Discussion 220
D. P. SHREINER & J. LEVIN Regulation of thrombopoiesis 225
Discussion 237
B. KUBANEK, 0. BOCK, w. HEIT, E. BOCK & E. B. HARRIS Size and proliferation
of stem cell compartments in mice after depression of erythropoiesis 243
Discussion 255
P. F. HARRIS Effects of anaemia on DNA-synthesizing cells in the blood and
observations on their origin 263
Discussion 277
H. s. MICKLEM,D . A. OGDEN & A. c. PAYNE Ageing, haemopoietic stem cells and
immunity 285
Discussion 297
M R. BLAND, J. F. LOUTIT & J. M. SANSOM Hyperplasia of myeloid and lymphoid
tissue (pseudo-leucosis) in mice bearing passaged sarcomas 303
Discussion 3 15
General Discussion
Population control 321
More about morphology 322
Intracellular factors 324
Extracellular factors 327
Colonialism 329
J. F. LOUTIT Epilogue 333
Index of Contributors 337
Subject Index 339
Participants
Symposium on Haemopoietic Stem Cells
held at the Ciba Foundation, London, 13th-14th July, 1972
Chairman: J. F. LOUTIT MRC Radiobiology Unit, Harwell, Didcot, Berks.,
UK
G. ASTALDI The Blood Research Foundation Center, Ospedale Civile, 1-1 5057
Tortona, Italy
P. MARY COTES MRC Division of Biological Standards, National Institute for
Medical Research, Hampstead Laboratories, Holly Hill, London NW3
6RB
K. A. DICKE Radiobiological Institute of the Organization for Health Research
TNO, 151 Lange Kleiweg, Rijswijk (ZH), The Netherlands
G. M. GRIGORIU Centre of Haematology, Str. C-tin Caracas, nr. 2-8, OP 2,
CP 1006, Bucharest, Rumania
P. F. HARRIS Department of Human Morphology, University of Nottingham,
University Park, Nottingham NG7 2RD, UK
G. HUDSON Faculty of Medicine, 304 Western Bank, Sheffield S102TN, UK
N. N. ISCOVE Friedrich Miescher-lnstitut, PO Box 273, CH-4002 Basel, Swit-
zerland
B. KUBANEK Abteilung fur Hamatologie der Universitat Ulm, Steinhovel-
strasse 9, D-79 Ulm (Donau), Germany
X PARTICIPANTS
L. G. LAJTHA Paterson Laboratories, Christie Hospital & Holt Radium
Institute, Manchester M20 9BX, UK
E. A. MCCULLOCH Department of Medicine, Princess Margaret Hospital, 500
Sherbourne Street, Toronto 284, Canada
D. METCALF Cancer Research Unit, The Walter & Eliza Hall Institute of
Medical Research, Post Office, Royal Melbourne Hospital, Victoria
3050, Australia
H. s. MICKLEM Department of Zoology, University of Edinburgh, West Mains
Road, Edinburgh EH9 3JT, UK
D. G. OSMOND Department of Anatomy, McCill University, PO Box 6070, 101
Montreal, Quebec, Canada
c. ROSSE School of Medicine, Department of Biological Structure, University
of Washington, Seattle, Washington 98 105, USA
D. P. SHREINER Department of Nuclear Medicine, Veterans Administration
Hospital, University Drive, Pittsburgh, Pa. 15240, USA
F. STOHLMAN JR. St. Elizabeth's Hospital of Boston, 736 Cambridge Street,
Boston, Mass. 02135, USA
D. B. THOMAS Department of Anatomy, University of Birmingham, The
Medical School, Birmingham B15 2TJ, UK
K. H. WINTERHALTER Friedrich Miescher-Institut, PO Box 273, CH-4002 Basel,
Switzerland
J. M. YOFFEY Department of Anatomy, The Hebrew University, Hadassah
Medical School, PO Box 1172, Jerusalem, Israel
Editors: G. E. w. WOLSTENHOLME and MAEVE O'CONNOR
Haemopoietic Stem Cells
Ciba Foundation
CoDvriaht 0 1973 Ciba Foundation
Chairman’s introduction
J. F. LOUTIT
MRC Radiobiology Unit, HarweN
The Ciba Foundation with its world-wide reputation tries to bring people like
ourselves together for fruitful discussions, which we must agree is an invaluable
form of communication in science. This particular meeting is devoted to Joe
Yoffey and the haemopoietic stem cell; it is about work in progress on a problem
not yet completely solved. By the end of these two days we shall know better
where we stand about the haemopoietic stem cells, but here I shall talk about
Joseph Mendel Yoffey whom we are honouring on this occasion.
To start in a rather formal manner, I quote one of his shorter communications
-from Who’s Who, where he writes that he was born on the 10th July 1902, the
son of Rabbi Israel Jacob Yoffey and Pere Jaffe. The rest of his career is listed,
starting in 1926-1927 as a research fellow, continuing as a research scholar of
the British Medical Association, a house surgeon at the Manchester Royal
Infirmary, through the usual stages of assistant lecturer of anatomy and senior
lecturer at Cardiff, and several Hunterian and Rockefeller professorships until
finally he was Professor of Anatomy in Bristol from 1942 to 1967. In this list in
Who’s Who there is a gap and as Yoffey is tremendously interested in numbers
and quantification, I am intrigued by this gap: what was he doing in those three
years?
I very much regret the absence of A1 Gordon, who was to have been chairman
on this occasion, because I feel he would have made a very much better job of
telling you all about Yoffey than I. In fact, I feel singularly ill-equipped, because
there are many people in this room who could have done it very much better
from personal knowledge. However, Yoffey and I have, by Brownian movement,
met on a number of random occasions over the last fifteen or more years and he
seems to me to be the epitome of the dedicated university don; that is, he has
two main attributes, teacher and research worker. I cannot speak about his
qualities as a teacher but 1 can speak about his qualities as a research worker.