Chondrosia reniformis; hitherto unknown bacteria

1 Pages·1999·0.21 MB·English

by  Schumann-Kindel G.

558 MEMOIRS OF THE QUEENSLAND MUSEUM
CUHNOKNNDOROWSNIABARCETNEIRFIOAR.MISMe:moHiIrTsHoEfRTthOe ausnidngstuhbespeoqluyepnhtasaincalaypspirsoaocfh.th1e6ScrulDtNiAv-atseedqubeanccteirniga
QueenslandMuseum 44: 558. 1999:-Marine sponges obtained from sponge-tissue showed that these
asevolutionarilyancientMetazoaarenowadays inthe metazoaarecloselyassociatedwithagreatdiversityof
focus ofgreat interest. However, the implication of hitherto unknown bacteria. Further studies of
closely associated bacterial populations within the culturable sponge-associated bacteria are currently
sponge tissue is completely unknown. One of the carried out to investigate the ecology of
sponges investigated to clarifythis relationship is the sponge-associatedbacteria. This surveywill elucidate
Mediterranean species Chondrosia reniformis. the physiological properties and, by molecular
Bacteriaassociatedwiththisspongewereexaminedby analysis, the phylogenetical affiliation of these
aerobicandanaerobiccultivation,culture-independent organisms.Occurrenceandspatialdistributionofeven
methods as whole cell in situ hybridization, PCR unculturablebacteriainspongetissuewillbeanalyzed
assisted rDNA sequence retrieval and comparative by in situ hybridization with specifically designed
sequence analysis. In situ hybridization of bacteria rRNA-targeted oligonucleotides. Porifera,
within the sponge tissue with fluorescently labeled Bacteria, in situ sequencing, phylogeny, PCR,
rRNA-targeted oligonucleotides for the major microecology.
subclasses ofProteobacteria revealed a great part of
the population to be affiliated to the alpha-, gamma- G. Schumann-Kindel (email: schu0654@
and delta-subclasses. Interestingly, no organism was mailszrz.zrz.tihberlin.de), M. Bergbauer, W. Manz & U
found to be a member ofthe beta-Proteobacteria. On Szewzyk, Technical UniversityBerlin, Dept. ofMicrobial
the other hand, there are also many microorganisms Ecology, Franklinstrafie 29, 10587 Berlin, Germany; J.
that only gave signals withthe universal probe forall Reitner, IMPG, University of Gottingen,
bacteria,whereasgroup-orspecies-specificprobesdid Goldschmidtstrafie3, 37077Gottingen, Germany; 1June
not hybridize with these bacteria. Some ofthem are 1998.
culturable and could successfully be characterised