Edited by Prof. I.P. Tripathi Published by Edited by Prof. I.P. Tripathi Pro-Vice-Chancellor & Dean of Faculty of Science & Environment Mahatma Gandhi Chitrakoot Gramodaya University Chitrakoot 2013 International E - Publication www.isca.co.in International E - Publication 427, Palhar Nagar, RAPTC, VIP-Road, Indore-452005 (MP) INDIA Phone: +91-731-2616100, Mob.: +91-80570-83382, E-mail: [email protected] , Web: www.isca.co.in © Copyright Reserved 2013 All rights reserved. No part of this publication may be reproduced, stored, in a retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, reordering or otherwise, without the prior permission of the publisher. ISBN: 978-81-83520-04-1 International E – Publication www.isca.co.in iiiizzkkzzkk--ss--ss dddd``""``""....kkkk ffffccccggggkkkkjjjjhhhh iiiikkkk....MMMM;;ss;;ss ddddqqyyqqyyiiiiffffrrrr eeeeggggkkkkRRRReeeekkkk xxxxkkkk//aa//aa kkkkhhhh ffffpppp====ddddVVwwVVww xxxxzzkkzzkkeeeekkkknnssnnss ;;;; ffffoooo''''ooooffffoooo||||kkkkyyyy;;;;]]]] ffffpppp====ddddVVwwVVww ¼¼¼¼eeee----iiiizz--zz--½½½½ xxxxzzkkzzkkeeeeffffoooo||||kkkk ddddhhhh jjjjkkkktttt////kkkkkkkkuuuuhhhh eeeess aass aa vvvvkkkkiiiiddddkkkk LLLLooookkkkxxxxrrrr ggggSSSS fp=dVw Hkkjr dh lkaLd`frd jkt/kkuh gSA Hkxoku Jhjke u s ouokl dky dk vf/kdrj le; blh lqjE; ifj{ks= esa fcrk;k FkkA dkykUrj e sa bfrgkl iq#"k jk"Vª_f"k ukukth n's keq[k u s fp=dVw e sa fofo/k izdYik sa d s ek/;e l s ;qxkuqdyw lkekftd iqujZpuk dk ,d ,slk ekWMy izLrqr fd;k ftldh lEi.w kZ jk"Vª e sa ljkguk gqbZA egkRek xk/a kh fp=dVw xzkekns ; fo'ofo|ky; ukukth n's keq[k }kjk laLFkkfir n's k dk igyk xzkeh.k fo'ofo|ky; gSA gekj s fy, ;g ^^^^xxxxzzkkzzkkeeee ffffoooo||||kkkk ddddhhhh jjjjkkkktttt////kkkkkkkkuuuuhhhh**** gSA fp=dVw e sa xzke fo|k dh bl jkt/kkuh e sa Hkkjrh; vkS"k/kh; iknik sa d s jlk;u] tSojlk;u ,o a vk;qosZn ij ipa &fnolh; jk"Vªh; dk;Z'kkyk ¼1&5 flrEcj 2012½ e sa i/kkj s lHkh yC/k&izfrf"Br oKS kfudk]sa fpfdRldk]sa vkpk;ksZ a ,o a 'kk/s k Nk=&Nk=kvk sa rFkk LFkkuh; oS|k sa o vfrfFk;k sa dk eS a ân; ls Lokxr djrk gw¡A esjk fo'okl gS fd fp=dVw esa vkidk vkxeu fo'ofo|ky; l s vkid s nh?kZdkyhu vkReh; lca /a kk sa dk vk/kkj cuxs k vkSj ;g izsj.kk Hkfwe vkidk s viuh ,fsrgkfld /kjkgs j vkSj lkaLd`frd eYw ;k sa d s izfr mnkÙk psruk fodflr dju s e sa mi;kxs h fl) gkxs hA eq> s fo'okl g S fd fp=dVw d s ifj{ks= dh oukS"kf/k;k sa l s ifjfpr gk s rFkk dk;Z'kkyk e sa egÙoi.w kZ fo"k;k sa ij fopkj&eFa ku vkid s O;fäÙo d s mé;u e sa egÙoi.w kZ dkjd fl) gkxsa As fp=dVw e sa vkidk s jkeHkfä d s lkFk&lkFk ouk"S kf/k;k sa l s rknkRE; gkus s dk jl fey]s ;gh 'kqHkdkeuk gSA ¼¼¼¼iiiizzkkzzkk--ss--ss dddd``""``""....kkkk ffffccccggggkkkkjjjjhhhh iiiikkkk....MMMM;;ss;;ss ½½½½ International Science Congress Association iii International E – Publication www.isca.co.in PREFACE India has over 3000 year old medicinal heritage whose main resource base was medicinal plants. We have perhaps one of the richest ethno botanical traditions in the world. Over 8000 species of plants of diverse habitats from orchids and ferns to trees, grasses, shrubs, climbers are used by local communities in different ecosystems from Ladakh to Kanyakumari and stretching to Northeast hills to Kutchh of Gujarat. Plants also became intricate part in various spheres of the human society. About 70% population of India inhabit in rural and remote areas of the forest and utilize a large number of flora for their routine requirements. Ayurveda is the ancient (before 2500 BC) Indian system of health care and longevity. It involves a holistic view of man, his health, and illness. Ayurvedic treatment of a disease consists of salubrious use of drugs, diets, and certain practices. Medicinal preparations are invariably complex mixtures, based mostly on plant products. Around 1,250 plants are currently used in various Ayurvedic preparations. Many Indian medicinal plants have come under scientific scrutiny since the middle of the nineteenth century, although in a sporadic fashion. The first significant contribution from Ayurvedic materia medica came with the isolation of the hypersensitive alkaloid from the sarpgandha plant, valued in Ayurveda for the treatment of hypertension and insanity. This was the first important ancient-modem concordance in Ayurvedic plants. With the gradual coming of age of chemistry and biology, disciplines central to the study of biologic activities of natural products, many Ayurvedic plants have been reinvestigated. In the early development of modern medicine, biologically active compounds from higher plants have played a vital role in providing medicines to combat pain and diseases. For example, in the British Pharmacopoeia of 1932, over 70% of organic monographs were on plant-derived products. However, with the advent of synthetic medicinal and subsequently of antibiotics, the role of plant derived therapeutic agents significantly declined in the economically developed nations. In the last two decades, there has been a new trend in the preparation and marketing of drugs based on medicinal plants. These preparations, labeled herbal drugs or phytomedicines, are single plant extracts or fractions thereof and are distinct from the pure chemical entities of molecular drugs. These new plant-derived products are carefully standardized, and their efficacy and safety for a specific application have been International Science Congress Association iv International E – Publication www.isca.co.in demonstrated. Thus, plant-based therapeutic agents continue to have scientific, social, and commercial significance and appear to be gathering a momentum in health-relevant areas. Indian medicinal plants are the essence of Ayurveda and Ayurvedic treatments, when used judiciously. Their role cannot be confined to mere duration of disease but they also used being of human body. Hence, Ayurvedic drugs are rightly called the elixirs of life. Ayurvedic Herbs played important role in Ayurvedic treatment, from ancient time to this most modern time. Indian Medicinal Plants/herbs shows good result on disease cure. Ayurveda is the medical/Health care system which uses this as treatment base with theoretical principles. We need to research many things to find out the pharmacological action of it. The advent of development exploitation of mineral and metal resources, construction of minor and major dams for hydro-electric or thermal plants, development means of communication, reckless hacking and cutting of forests tracts for rehabilitation and agricultural purposes are having telling effect on the plant resources of Central India. The forest area are gradually shrinking due to population explosion and increasing demand on forest resources for sustenance of human at large. A large number of medicinal plants may then disappear prematurely before their inventorization, assessment and utilization of potentials for human welfare. Sustainable utilization and conservation of medicinal plants is the demand of the time. This workshop aims to discuss and practices all the issues pertaining to medicinal plants research, documentation, utilization. conservation activities, standardization, quality control, tissue culture, biotechnology, biochemistry, phytochemistry and chemical characterization taking place in various Universities, Institutions, Colleges and their impact on medicinal plants. Thirty five abstracts related to chemistry, bio-chemistry and Ayurveda were presented and discussed in the workshop. An excellent practice (tissue culture, identification of plant, instrumental techniques etc.), Harbal garden & pharmacy visit and marvelous field visit was also orgnised during the workshop. I hope the proceeding will catalyze activities for further research and practices of all the issue pertaining to Indian medicinal plants. Prof. I.P. Tripthi International Science Congress Association v International E – Publication www.isca.co.in ACKNOWLEDGEMENTS We humbly present our gratitude to Honourable Vice-Chancellor Prof. K. B. Pandeya and Padamshri P. Puslpanghan for grateful inauguration of the National Workshop on Chemistry, Biochemistry and Ayurveda of Indian Medicinal plants amidst a September gathering. We record our special thanks to right Honourable Vice-Chancellor Prof. K. B. Pandeya for his keen interest in the workshop and an encouraging foreword for the proceeding. We are very thankful to Dr. R.R. Rao (FNA), Vaidya Jamuna Prasad, Dr. Bharatandu Prakash for her splendid address delivered in the inaugural ceremony. Our sincere thanks are also to Honourable Sant Chola Baba, Satianusuia Ashram Chitrakoot and Dr. K.V. Billore, Prof. T.R. Sahu & Dr. N.C. Shah for the valedictory address in the workshop. The proceedings are outcome of cooperation of the authors, reviewers and many people at different stages in different capacities. We are obliged to Prof. Aroop Kumar Gupta, Presently Vice-Chancellor & Dean, Agriculture Faculty, Prof. S. S. Sengar, Dean, Art Faculty, Prof. R.C. Singh, Dean, Management Faculty, Er. K.P. Mishra, Dean, Eng. & Technology, Dr. Ajai Kumar, DSW and Prof. K.D. Mishra, Director, Research Directorate for encouragements. We wish to thankfully acknowledge the support and cooperation of Dr. Ravindra Singh, Dr. Vandana Pathak, Dr. Shudhakar Mishra, Dr. S.K. Chaturvedi, Dr. Sadhana Chaurasia, Dr. R.L.S. Sikarwar, Dr. Sachin Upadhyaya, Dr. Vijay Pratap Singh, Dr. S.P. Pathak, Dr. Rakesh Shrivastava, and Dr. Reetu Sharma. We also thank to our departmental colleagues and students for their active cooperation. The conference was financial supported by various organizations and we again record our thanks to University Grant Commission-New Delhi, DRDO-New Delhi, M.P. Council of Science & Technology-Bhopal and Bundelkhand Extended Region Chaper (NASI) Chitrakoot. Our thanks are also due to Academy Press Allahabad for excellent and timely printing and to Shri Bramhanand and Shri Vishnu Kumar for appreciable computer layout, and keen interest in the publication. We deeply appreciate the sacrifices made by our family members who granted us the time we required to organize the conference and to complete the publication of this scale. We specially mention our researches & Post Graduate students Sarika Singh, Arvind Dwivedi, Sanjay Saxena, Neelesh Dwivedi, Priyanka Gupta, Noopa Dwivedi, Mahendra Mishra, Atul Dwivedi, Arti Kamal, Ruchita Tripathi, Chinmayee Mishra, Kailash, Anjnay etc. for their patience and cooperation. Prof. I. P.Tripathi International Science Congress Association vi International E – Publication www.isca.co.in HHHHkkkkkkkkjjjjrrrrhhhh;;;; vvvvkkkkSS""SS""kkkk////kkkkhhhh;;;; iiiikkkkSS//SS//kkkkkkkk sasa sasa dddd ss ss jjjjllllkkkk;;;;uuuu]]]] ttttSSooSSoo jjjjllllkkkk;;;;uuuu ,,,,oooo aa aa vvvvkkkk;;;;qqooqqoosZsZnnsZsZnn iiiijjjj vvvvkkkk;;;;kkkkffssffss ttttrrrr iiiippaappaa ffffnnnnoooollllhhhh;;;; ddddkkkk;;;;ZZ’’ZZ’’kkkkkkkkyyyykkkk ddddkkkk iiiizzffzzffrrrroooonnssnnss uuuu yxHkx 5000 gtkj o"kZZ iqjkuh ekuo lH;rk e sas Hkkjr o"kZ dk iqjkuk fpfdRlh; Kku gS tk s ouLifr;k sa ij vk/kkfjr Fkk vkSj 'kk;n mlh egRo dk s /;ku e sa j[kr s gq, Hkkjrh; lH;rk e sa ikS/kk sa dh itw k vpZuk dh tkrh Fkh ftll s mudk laj{k.k gk s ld s vkSj mudk foLrkj o izo/kuZ gksrk jg s ftll s ;g fojklr gekjh vku s okyh ih<+h dk s Hkh fey ldAs vk;qosZn i)fr gh euq"; d s lEi.w k Z LokLF; ,o a mld s nh?kkZ;q gkus s d s fy, ,d lEi.w k Z Kku gSA fodkl dh nkSM + ,o a ik'pkR; lH;rk d s lØa e.k d s dkj.k gekj s ijEijkxr Kku dk /khj&s /khj s ykis gksrk tk jgk gS vkSj ge vk/kfqud cuu s d s iz;kl e sa bl /kjkgs j dk s fdukj s djr s gq; s c<+r s tk jg s gAS A bue sa e[q ;r% gekj s vkS"k/kh; ik/Sa kk sa o [kfutk sa dk fujarj àkl gk s jgk gSA ekuo lH;rk d s fy, izkd`frd :Ik l s mld s 'kkjhfjd vko';Drk ,o a izdf`r d s lkFk lketa L; cuku s okyh vk;qosZfnd fpfdRlk i)fr dh vksj /;ku vkdf"kZr dju s gsrq ;g ipa fnolh; dk;Z'kkyk vk;kfstr dh x;h ftle sa fuEufyf[kr igyvq k sa ij foLr`r fopkj foe'kZ fd;k x;kA 1- vkS"k/kh; ikS/a kk sa dh igpku] mud s ,d=hdj.k] izkslfslxa ] lrr~ miHkkxs ,o a laj{k.kA 2- vkS"k/kh; ikS/a kk sa dh jlk;fud lajpukA 3- vkS"k/kh; ikS/a kk sa dk vk;qosZn] ;uw kuh ,o a gkEs ;kis FS kh e sa mi;kxs A 4- vkS"k/kh; ikS/a kk sa dk ekudhdj.k] xq.koRrk fu;a=.k] vkS"kf/k fuekZ.k ,o a mudh lEHkkouk;Asa 5- fV'kqdYUpj l s vkS"k/kh; ikS/kk sa d s fodkl ,o a laj{k.k dh lEHkkouk;Asa dk;Z'kkyk d s izFke mn~n's ; ij ckys r s gq; s izk0s - Vh-vkj-'kkg]w izk0s vkj-vkj-jko] izk0s d-s Ogh- foYyskj s ,o a Mk0 vkj-,y- ,l- fldjokj u s crk;k fd fp=dVw e saa vk;qoZsnh; vkS"kf/k ikS/kk sa dh yxHkx 300 iztkft;k¡ miyC/k gS vkSj mud s laj{k.k d s fy, i;z kl djuk vko';d gSA Mk0 HkkjrUs nq th u s crk;k fd P;ouizk'k e sa iz;qDr gkus s okyh lHkh pkyhl vkS"kf/k;k¡ fp=dVw es a mifLFkr gSAa fp=dVw tSo fofo/krk d s fy, ,d LoxZ d s leku gS vkSj bl izkd`frd okrkoj.k dk s lajf{kr dju s dh vko';drk gAS dk;Z'kkyk d s f}rh; mn~n's ; ij idz k'k Mkyr s g,q fo'k"skKk sa u s crk;k fd vkS"k/kh; ik/Sa kk sa dh igpku jklk;fud lajpuk dk v/;;u dj mudh okLrfod igpku dju s ls mud s lgh mi;kxs esa lgk;rk fey ldrh gS o bll s mudk mi;kxs xq.kdkjd vkS"kf/k;k sa d s fuekZ.k e sa Hkh fd;k tk ldrk gAS Mk0 ,u- lh- lkg u s dk;Z'kkyk d s rhlj s mn~n's ; d s vUrxZr viu s fopkj O;Dr djr s gq; s dgk fd Hkkjrh; vkS"k/kh; ik/Sa kk sa dk s tk s LFkku feyuk pkfg, og gekjh mi{s kk d s dkj.k ugh a fey ik jgk gS] vkSj vk/kqfud fpfdRlk i)fr dh vksj gekjk vkd"kZ.k c< + x;k gSA dk;Z'kkyk e sa vkS"k/kh; ik/Sa kk sa d s ekudhdj.k ,o a mudh xq.koRkk ij fo'k"sk /;ku j[kr s g;q s mUg sa fo'o Lrjh; nokb;k sa d s :Ik e sa fodflr dju s dh vko';drk gSA International Science Congress Association vii International E – Publication www.isca.co.in tSlk fd vkidk s fofnr g S fd vkt txa yk sa dh v/kk/kqU/k dVkb Z ,o a Climta e Chna ge vkSj fge[k.Mk sa d s u"V gkus s d s dkj.k cgqr l s vkS"k/kh; ikS/k s lekIr gksr s tk jg s gASa ,d losZ{k.k d s vuqlkj iRz ;ds 1 feuV ij tSo fofo/krk l s ,d itz kfr foyqIr gksrh tk jgh gAS ;fn bl foukl dk s jkds uk gS rk s mu itz kfr;k sa dk s tk s foyIqrh d s dxkj ij gS mud s egRo dk izpkj&izlkj] [ksrh o Tiuss e culture }kjk laj{k.k o lao)Zu dj mUg sa lajf{kr dju s dh vfr vko';drk gSA bl mn~n's ; ij fofHkUu fn'kkvk sa e sa py jg s iz;klk sa dk s Hkh dk;Z'kkyk e sa oSKkfudk sa u s crk;kA dk;Z'kkyk d s nkSjku LFkkuh; QkesZlh] vk;qosZn laLFkkuk sa rFkk ouLifrd m|kuk sa dk oKS kfudk sa ,o a Nk=k sa }kjk Hkze.k fd;k x;k rFkk ogk¡ dh xfrfof/k;k sa dk s le>k x;kA blh nkSjku fp=dVw d s txa yk sa dk Hkze.k dj vkS"k/kh; ikS/kk sa dh igpku dk s Nk=k sa u s tkuk rFkk mud s xq.k o mi;kxs d s fo"k; e sa xgjkbZ l s foe'kZ fd;kA mijkDs r O;k[;kuk sa o {ks= v/;;u l s fo'ofo|ky; e sa fo"k; lEcfU/kr yxHkx 300 Nk=k sa dk s viu s Hkfo"; ,o a viu s 'kk/s k fo"k;k sa dk s pquu s d s fy, fn'kk funsZ'k Hkh izkIr g;q As ddddkkkk;;;;ZZ''ZZ''kkkkkkkkyyyykkkk ddddhhhh vvvvuuuuqq’’qq’’kkkkalalalalkkkk;;;;%%sasa%%sasa&&&& 1- fp=dVw tSofofo/krk dk Lox Z gS] ftld s laj{k.k dh vfr vko';dRkk gSA ;gk¡ vk"Sk/kh; ikS/kk sa d s ,d=hdj.k d s fy, LFkkuh; ykxs k sa dk s izf'k{k.k nus s dh vko';drk gS] ftll s vkS"k/kh; ik/Sa kk sa dk fouk'k jkds k tk ldAs 2- vkS"k/kh; ik/Sa kk sa dh [ksrh d s fy, LFkkuh; fdlkuk sa d s legw cukdj mUg sa ifzsjr djuk] ftll s mud s lkkeftd&vkfFkdZ (Socio Economic) Lrj e sa lq/kkj vk ldAs 3- vkS"k/kh; ik/Sa kk sa dh izkslfslxa dju s d s ckn gh cktkj e sa Hkts k tk;] ftll s fdlkuk sa dk s mudk i.w kZ o mfpr eYw ; fey ld s vkSj mldk lrr~ mi;kxs gk s ldAs 4- iknik sa d s igpku e sa mudh ,o a jklk;fud lajpukvk sa (Phyto-chemistry) dh egRoi.w kZ Hkfwedk gAS bl rduhfd dk iz;kxs dj vkS"kf/k fuekZ.k e sas lgh iztkfr d s mi;kxs dk s lqfuf'pr dj sa ftle sa nokvk sa dh xq.koRrk c<x+s h rFkk tulkekU; dk dY;k.k gkxs kA 5- vkS"k/kh; ik/Sa kk sa d s vk;qosZn e sa mi;kxs dk s c<+kok nus s gsrq LFkkuh; QkesZlh fodflr dh tk; ftll s nokvk sa dh xq.koRrk cuh jg s vkSj vkS"k/kh; ikS/a kk sa dk s mfpr LFkku fey ldAs 6- fo'ofo|ky; e sa fV'kdq Ypj iz;kxs 'kyk dk fodkl dj mu vkS"k/kh; ik/Sa kk sa dk s izof/kZr (Propagate) djuk pkfg, tk s foyIqr izk;% ;k foyqIrh d s dxkj ij gAS bUgh a vuq'kalkvk sa d s lkFk lkSgkniZz .w kZ okrkoj.k e sa dk;Z'kkyk d s leLr rduhdh l= lEiUu g;q As llll;;aa;;aa kkkkttssttss dddd jjjjkkkk""""VVVVªªhhªªhh;;;; ddddkkkk;;;;ZZ''ZZ''kkkkkkkkyyyykkkk International Science Congress Association viii International E – Publication www.isca.co.in CONTENTS PART-I IDENTIFICATION AND CONSERVATION OF MEDICINAL PLANTS 1. PHYTOCHEMICAL CHARACTERIZATION OF MEDICINAL PLANT IN HERBAL DRUGS 1-12 P. Pushpangadan, Vipin Mohan Dan, T P Ijinu and V. George 2. MEDICINAL PLANTS of INDIA: DIVERSITY, CONSERVATION AND 13-40 BIOPROSPECTION- A PRIORITY AGENDA FOR 21ST CENTURY DR. R.R.Rao 3. UTILIZATION AND CONSERVATION OF MEDICINAL PLANTS IN INDIA 41-49 Dr. K. V. Billore 4. WHY THE INDIAN MEDICINAL PLANTS COULD NOT FIND THEIR PLACE IN THE 50-60 MODERN MEDICINE? AN OVERVIEW N.C.Shah 5. MEDICINAL PLANTS IDENTIFICATION AND IMPORTANCE OF HERBARIA IN 61-66 MEDICO-BOTANY Dr. K. V. Billore 6. PROSPECTS OF CULTIVATION OF SOME IMPORTANT MEDICINAL PLANTS 67-78 S.K. Tewari, Shweta Singh, S.K. Sharma AND R.S. Katiyar 7. PHYTO-PHARMACEUTICALS AND AYURVEDIC NORMS 79-86 Sanjeev Kumar Ojha: CH V Rao: Sri Krishna Tewari 8. CHITRAKOOT-AN EMPORIUM OF BIOLOGICAL AND CULTURAL DIVERSITY 87-93 R.L.S. Sikarwar PART-II TISSUE CULTURE AND BIOTECHNOLOGY OF MEDICINAL PLANTS 9. ROLE OF PLANT TISSUE CULTURE IN CLONING AND CONSERVATION OF 94-108 PHYTODIVERSITY OF SOME ECONOMIC MEDICINAL PLANTS OF INDIA A. K. Sharma, Kavita Arora and Meena Sharma 10. IN VITRO APPROACHES FOR MASS PRODUCTION OF MEDICINAL PLANTS 109-116 Pragati Misra and Pradeep Kumar Shukla 11. IN VITRO CONSERVATION OF BIODIVERSITY OF MEDICINAL PLANTS IN INDIA 117-122 Reetu Sharma and S P Mishra 12. THE WONDER PLANT AMARANTHUS–PHARMACOGNOSTIC PROPERTIES AND 123-129 APPLICATIONS Kavita Niraj, Ravindra M.Samartha and Puneet Gandhi PART-III MEDICINAL PLANTS IN INDIAN SYSTEM OF MEDICINE- AYURVEDA, UNANI AND HOMEOPATHY 13. ANTI-DIABETIC PLANTS 130-133 Dr Prakash L Hegde 14. THERAPEUTIC USES OF COMMON MEDICINAL PLANTS 134-139 Prof (Dr) G.S.Tomar 15. MORINGA OLIFERA OR SAHIJAN – A MIRACLE PLANT OF MEDICINAL VALUE 140-144 Dr. Vinod Kumar International Science Congress Association ix