Characterization of SSRs from the ESTs in the wheat midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae)

2011·3 MB·

by  Yun, Duan

1m San ct Siar, ne. GY PSL ss ate

ZAR HER EST-SSRs (5 BAHT
RDF he io hse
Ane.

BUYIN, Bola RTE
1 Laketirhbss ues Peewee
SaRARM IRB eo, ARR EERO

Ra SLA INTEL ce RA oA EARS, AT NC Ry I RU RAT Ze
BEM neo move Bre 27 BS EAE TF wine SE, SSPE LO AME PE
aM Tigre SRA 1 SSE RAE 8 12 jes, ESA HL
fresh eh, Ge AAA al, RAE GUL, Les IT LL ATH 2
SG HIS A AAC p= HER HA PED, Hl ond.
fic tas estosne SesI LF PSTN MR DRS 2AM, ZS £980 2 Ris
FAL MAG 20 7.826 OLE RT VE, THES A AMEN ST BT
SEAL, hoy IST fk, RALPH, EAD ARTE, OES
EO, FEARS HE He), AE TAME SL TAN PSHE URS PTT
SHOT, TMI LAIR, Ia Ss Hi
PME. Oh, ARRAS A SETI, seteCUN SS LAR
characterization pf SSRs Icom the ES''s in the wheat midge, Sttodipinsis
moxellana ( Gehin) (Diptera, Cecidomylidae)
DIAN Yar °. WU Rew Mi’, TRO Zhi TEE Tu Qing. TANTS Ye 3S, WIAD fe? CONE:
boogJox? CL. Saw Ker Labntory fir Uioley of Hw Disesiee and Iscet Post. loaiue of Mant
Prams, lives Arles of Shun Seve. ifs IOOISA, Ching 3. He he Laburaey
cf Crop test Conn], kustere of Mau Hoteton. Henan AcaZeuy of Agicuhal seieuces, Zhevzon
451062, Cr
Aburuet; sect copes sequence tae LSI¥; dsabneeplordes a valunle seo fr
ella lar whet. Lin wn,

lpi,
ESTs Ta alah ho a

rips, Skudisier mesilane, nore denis fm KURT daalase aad esol data sagan The
laueluintioe sf wigunodainad SSI wo aad al yutal ESIESSIG wae seca The sea lle
smal thar IML RST S6Re silted as 105 (10, 1346) seigary smyuewens were deme, Ht
rota ace SOM evesy 3.49 Kb af uaas querer. laos 1~6 oudsolde sepeal «yee. L = 3
velba ep ae Le wre Ite, searing la 97 168 af all SER ACT, ACT al AACCTT
secouut fer the hiphiet poopertion in mccenuclostide. dimucleotie ard tinacleotide, respectively.
Fictirmme, HST-SSI pine ie woe design: ung te Pier Pri 3.0 pyran el 26 ps ee
telvetd ior masker development in % masons adhe, OF the 26 pa pais. 20 pate 76. 9

Pade swipes + a rention PER yrabite, al of ve 20 wriner pi, psi
1456 nplayed pogo, Hiren alleles wer fund io Zar 9 price pie. the ancan aoubee of
lle per im 5.87, the verge async Utena UL 63 aa tn eine pb ni

Anoration content | PHC wae 160, The SSR denied fe ahi tad il aly ear te palin
fosetie eure al patie vig oS sores i, bs we “ae
Fey wos; Stoica muntionag eens seponan te, FST SSR; molar mor; pls pion

Fai A BNO mana

Fait ae. eS, THELIAL
ab eh

evan

Deka BEER gs Sha
has nase

AE DNAY esate ie DNAS ELT 8
TRE AEH were
repeats. SSKs) IRAE ALAS IML
Sh GEL WLR. aI REE HeMMBO
ROME SCAIGS, 2007; Wane, DDIM
BR DSA CBI SAC, Hal Chaba EL
Wa, He Mee. REE, HTL
Re UT aL RANE HE AGT LTE
WEED BSB AVPH Quah ou,
2008; Usa. 2006; RE
2008; Klin a, 200%; 3 PUPP, 28
Shura, 20095 Foblmger tal, 2103 Bee
RAM. ENGEA SL LEAL, Je
2B TE BU AL aL PF PE Be
Cates wal, 2007),

#

wae.

BRAS aH
1, TD PHT St bi HW AT
SEMA. 283. I, SOA BITE
TEMA, SE RAMMED ER
BM UN NERA GRE
oon, IN, RANE HDD MAS
Ame ATONE. SAAN
AL 8 UNA EEL ATER, BORAT
(97. RAR: expend sean tgs
TST EAR SR ILEMER DET oa
2165 isan Boe, 2007) Ff BESE
AE BEAR BAAN Mae a, 2810)
MAL. th PPRUGAMTIL, SWEAR.
Hida Sm TE a an A
ATHALIOA, EBL ED. ARS
SALAM, HSU SPT BDA
AER, RL TST AMER. JT
SOU SRIPC ATER. A, HACC,
‘AUGER S. OR USES PL
3 ae. PIL A, EH
Piece tog OSa12 AHL RR RUB!
adnan signe sade nat WARS 6
‘rns owen CR NHB eA
2, ng to, TOO, Kin a SOR a a
25,2008, Arun oo, 20095 ANAT
20>,

“REL AEN Sedo mstne -ASPLAB
PIERRE, WER, Bee teae
FMAM, RACAL ERE

EM GGR TA MARIS, (ARR ID:
FH RATA CREE. 2am, Fe
1. UT: Lituhae fof, 0021, FURL. ERAT
RIL OS 3 82 RAP Seo a

aE CHLAN, He al Yew, 2001, He
TSE, O07), eS HeEREN rem 8 Sk
BIS PAWNTT, TRAD PT
SERA, Asta) ae RST EPLALAT § SSR
He MLSRBSE. Sey BriLb He
Hh BHAA 4 14 JT RELATE IRL
BERLE SrUNCREHLET,

1 HES

La gabe
210 T= 5 MARA Za ea Ad
AEA IS AMR SE

“LAF PED, HLM =
‘ats ton RFF PILATE. MGW HAITI
SEWALRAR, TIT AZT. -wese
etm,
2 RSTS6R AER

PASTA Fob A NCL PREZ
kil lb oe-nuea FAL LIRA 2
KE LT & LSE HF. WAL commen
Repeainkes ASEAN. HEA che
ARI FAESEDEIEA Swipes A: A SST
Supe sequezes repeat ideation tual) Kt
Chm. mene. glare 2

AY anes 324 SSH AMER, cob:

STALE BID Lh, ITAL TREE B=
RMU LOE SAL.
La Sem sfabsaet Sort

‘Hla Princ Pane balls, 20h

SOARS ASL EST I HUES JIA
Hum RST 23) [GEA 100 bye SSR LH,
ASEH ACE ASME S44 KT 2 bps

CHE 1B 2 bps We AIRIE L AL W
6840 LEAT: I ERT A |
SC, CC feat aMey AO | POR EASE It ~

400 ny LIS BRB VLE AE
ir

Hair, Pae winer BSG ASTRA HAIN
SM TIRE: RL
14 Bale DNA

PL ode RUGABLAE DMA FNM RT BE
STREESRE Os BATRA MBH HA EAL
TRAE Nak, Be ECA. RDS mb
ESTAS TRM!, AREY HHO 92 1K, MA 20
LAT DNS AUR BEETS SI, 50 snl, THC

GMS, 25 oumW)'L Nat, BS ouwoL-L LUA)
ALUM UTE RGU. MA 30 pT DNA ae
HOM, TT osc vein, MAS

‘ae (oI. 2) 10U pL 3A" LD ih, 12 Ue
om. 2 10 vies ALEPH AS I

AUS RBUIOS HE AAkZ AON ALS. SE
20 ALL 12 00 aps PED 18 a, At
Ein, RAI, FTO ZvBEA a
POURS ie, SPUTA IS al MLO, seh
FE. UTA
LE SSR ACR ¢ PREM

TOR Fie SFR (10 39s 10 2 hates CMe"

LMP D.B yal, ANTS £2.

Pres Vs 25 a

maml/L? 1 pL, RSESIAICIG pmeL40.5 yh,
THA prot AHS. S pT. ATO 8, 6 yy Pog
DNA EAE 5 L2pL) AE Tahalie Boye)

U1 pb, HOHE U.S pd, PCI AEH Soosusa
Tabtgeler. 2 PCR RL UT, LEE Fe SH
WAHL rin, OCF. 90 0, H1 SET EF. Ue,
OL Vin 38 AM ASMES PLE 720 10 in SEA

Em,

PEACE, TRIO wR
PRS

RASHES POR
SMA? pL 6 DN |
TT os ban ici. p
ATR APM TELA ICES LE
Lox TRE, RiktaR SHIRES EASA
tna, 4
16 SARS SOE

SU ae -P —tcotNA BTL
esd TON SORTA HR 8 10 = 200 Un
HHINESTIAND. BESO hy TS laden RFS FAT
A ainn?. 0.1L gam 4,7 SERGI T We
FLARE Ltat, WOR NRHA, By
ALAS Lopeenet® (rerson LMI Yeh et a
19997 LWA EN EA THR
allie 0) Hl AEC) A

SE MURLATSTSST sn GATE in

Pie
PIC}

sion D029 7 AE ARE Bd

2 BRS

201 SEARO UST-SKe (ARE
ARES Mh UMBDNB, 207: PST FPA
AUALSE ATSC SIME, SER. 1 194 SR ALT
FSP HEIAL | 189 > naigeret ht E129
saanige AUSUR tse), RUE SER 42. &
PPI 470.71 by. EL SOIT Pees eae
SSI, SUBSEA LAD 4p SERS. OME 108
sy unigenca I, TESS 3.49 Eh GAIUS, SSI
FUT RRO, LZ
8 48. BI)
Pa
2.2 EASE BST.SGT Ara ETO
aE
AEE L faites, BERRI EST

PALE. GANT Ek a elepeAt
PAE AGAR SEE, PELL 3 ANE
SIL WE. diff ESESSK (EOF 6 ELS, TE
RAMEY ATS LR. HS a
GN). MC NRA he i BAS
(31.919), PENRO
131.216), 1-6 AREER, Ars) |
PRO. TISE), LO. F1HE 2 RELA

Fl RERUN ANE THLR
“Table 1 Reoweace ef leo sepa au of the
[BST-S5Ks i the salary shad of Sodio moslons
36 nase
FEL A 81eb, JE 2 ee AeE a.
BOTH IWR WI A Hh OFS
35 ART, CHER
BLACK SLR, al C-CC2. SE TN
Aga ALM Tl
C1S.608 3 RATA Ao, HO
(BSUS) RITE ATPATEA. AS LG
ARMOR HL, ata ese
CUT ABLE, aE 8. 2086), RHE AC
(7.10%), MERE AACVETTES. COS) FOUR
hidd #9, AGIE, uaa, alata,
ASCAAA, BUI
22 AORROEM TST Sm EEN CERO
Table Freayene of a gc nls oft RST-SSR
An slr nda Sottls wontons

rer

2.3 RATRESL IDA RST.SSR 3]
“HG Prine Pre ier 5. Sead A SS AL
6) FST SF TELS. LT RAISE,
Pm RET AER ETAL
Ae ll 26 ab ig aSE S
Coealn
es M262) SSE ROOM ISH
Ai, ARENT, MHEL” 967 HRTETS DNA et
FE BU LTO. IRI ob

MAA Th, FG ate ARSED,
DLS, Suet ARE
HU,

[HG 8-H
ADS] HOR RASA TI
CRISIS REM, MEDAL 14H

cl

BATVAALRSL AML, 20 21 USS 41H
BAUER SOIL MD ETL A DNA Ts. RD

SASSER OOH, WEA BL aR
9 sl FST 85m 3149 Fa
ASAI, FARE SI
SOM, SUMP, TYRE,
1B S.6P, AREA TAY 2S, MTEL ee
PROT ~0.86, TOE RSA. S, BARE

PSO OM, PIERS GR NOR,

2. 1, peeveee ADR? RAINES
fy, Ath? 3] STS GLE HEME NS
at

3 ate

AUS HEARD boil, BASE
FRO HRS, RL DNA Ate 7r
UTS AET SAE RES
FF. 10; OIEAF, 2010, Mn os, 2011)

CREAMS S SLE. Ak SEAN

RMR WS-SSU bY TZ. JeH 5 SB RAL 1 CHT
igen eB LAT SSS EEA SEE FN
nigries HE ABSTAEA. A Lb Abs — 4 S8R 2

Ante b, BLT 3 IG, SEARLE SAME
SOL USEASK (97.168 LE, FEL, SARS.
FECHA), Moka AAR
AEE 91 ML RRL MeECa21Ej
4 6 TATE TE LMSC 2 BAe TT
SENET, (LE Ras WEIR UST-SOUC
AT, AAT A AACSOTT Pe at I aa
SERIES NR RH, ea SHEL
GALS. Augen, AURA. cates SVG HU
1 Sciserimes wont SRT UC FEA,
KAHAEST, DOM, LT MRE RETEHK, 20m

FOU, 408 TU, 1A RE, HL
AT, ALCS AA-CT SRI. aR
Kab AM he CL, TR.
ARLOMMPLSAI, KEIRA At
ERG AIR, TR
(S420 1 Wee he ON, 59 a
120.608 REAR,

Mun DMA BA See. HPA TOTHl
UAH AAT OR CEE, 8 Z:
SU RALIEG FST SR AES v sles. FST
SS AD PCH IPH bSi, SETI
ir

Rs eters Isr

SE MURLATSTSST sn GATE

Re aot Panacea Fen reer)
ane reese uncut, iast (Gate “

TRE IS

FP ATIREA GEL 7 the pion salons 8 mer noe ope hm ara
"Oat

Ss IG FEMA

LDR

1g. 1 Tae ampli ale Sods ha Bang, He

i ws Rs

ms

ree

vase ape POR
SE ples OE C4) ad EC
sli

Ra RMR URIR EST Ey ssi CIRO
“oe 4 Game characte lin SSR deriva rm FST in thy lary sland of Sic mation

a AOVRBE WAERMA BtRaD ANAER EREAST
ta - @ as ae es

Hib, USSSA RIG ATE Mens oa
2009; BA EUIRAERED (020 ETP BRE
SKF BL HG IIS | KE
Sea A A 5. EMAL SEE a BO
FREA RENO, HI REE RA
1.60,

ALARA Ih. ALR AE en DN
SPRITE Lege a, 2OUE,
Pralcr wid Fine, 200 Borin a. 2010, he
AGT Rsk HALAS UNA BT
ATs JME. Gath ORC
SIpReT luce TEE STE, ER
ESL PW HUIS HLT RL TREE

© at [Reference

DR RAT. ae mes Tah

eee
ir e

Isto, Ja. he MERE
ean sRIL LM Es

sage re hee Hts doen ee ewe

Scns PET 99. LR Aa, 2,

CSIR ABE ASCII, BH

et hi et a

Fk AR.
£30, S10, CES EE
eaWR isi 12h a

vse eae ei i Ces te:

% 30,040, tan Eh fom) ean
Iie VM give ey aeehiol aes

HR MU, bn tn FY, 38 ann

WA ets RULE. ad AME. MAW RSH
Lem. DESH © C1 Hn

een icin taste, 2s 8 1 ke

NPR OER 0, S17) ARROLURI Aa
HREM. aRRS. ote

evaee gen ies (eta Deheies
rate Sng SOE AL. 38, Ral
Bal Sr ES Be Kee,

sed ANIA FT

ig BH TAC» at 2. See asec pe es
ie it EP a MG,

on Say LATS
4

Hh ARNOT A

pee eth eal

Fen Bien 8-0 140
A 2M6. Sha AAPA Le RE
ari uate, W163

oui, Sa 2, Kot, sae 20

tee: ate

Mohan, 9°52:688-85, ITE, it HE MC a
Pabacgp een

lois visa ea

6-72 ae AR.
BON, TARTAN 98

Lier THR IRS AE Leer 27
onl

ee
st ao

eR SSMS LAR HL MERI, A.

a Soe Sen TEENS WY AS. TE,
PPL MG MERLE CTD ER
BR. Whe Pat ARAFR eae oT

‘inte Pei: tt aie tin, Qk

Sams. rs Bote Semin, 338 BL 15
Fs, AS KR. LAME he. IEG EI MOLE

Is AMSA. RMD OS os a

inh, tom Enns an, 834s PRS, LACM
SRI. Revco Trea
ESTURE ENPH, G2 0

ores