Cell-Free Translation Systems

253 Pages·2002·9.921 MB·English

by  Alexander S. Spirin (auth.), Prof. Alexander S. Spirin (eds.)

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A.S. SPIRIN (Ed.)
Cell-Free Translation Systems
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Springer
Berlin
Heidelberg
New York
Barcelona
Hong Kong
London
Milan
Paris
Tokyo
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A.S. Spirin (Ed.)
Ce -Free
ranslat-on Systems
With 91 Figures and 17 Tables
Springer
Prof. ALEXANDER S. SPIRIN
Institute for Protein Research
Russian Academy of Sciences
142292 Push chino, Moscow Region, Russia
ISBN-13: 978-3-642-63956-2 e-ISBN-13: 978-3-642-59379-6
DOl: 10.1007/978-3-642-59379-6
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Die Deutsche Bibliothek - CIP-Einheitsaufnahme
Cell free translation systems 1 Alexander S. Spirin (ed.) - Berlin; Heidelberg; New York;
Barcelona; Budapest; Hong Kong; London; Mailand ; Paris; Santa Clara; Singapore;
Tokyo; Springer, 2002
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Contents
Part I
Introduction
CHAPTER 1
Cell-Free Protein Synthesis 3
ALEXANDER S. SPIRIN
Part II
Improved Classical (Batch) Cell-Free Translation Systems
CHAPTER 2
Improved Batch Translation System Based on E. coli Extract 23
THORSTEN LAMLA and VOLKER A. ERDMANN
CHAPTER 3
Improved Composition and Energy Supply
for Bacterial Batch Systems ............................. 41
DONG-MYUNG KIM and JAMES R. SWARTZ
CHAPTER 4
PURE System: New Cell-Free Translation System
Reconstructed with Purified Components ................. 53
TAKUYA UEDA, AKIO INOUE, MIDORI KAIDA,
RYOKO BABA and YOSHIHIRO SHIMIZU
CHAPTER 5
Direct Expression of PCR Products
in Cell-Free Translation Systems ......................... 61
ANATOLY T. GUDKOV and KIRILL A. MARTEMYANOV
CHAPTER 6
Yeast Cell-Free Translation Systems ...................... 67
MICHAEL ALTMANN and HANS TRACHSEL
m
Contents
CHAPTER 7
Poly(A)-Dependent Cell-Free Translation Systems
from Animal Cells ..................................... 79
GIOVANNA BERGAMINI and FATIMA GEBAUER
Part III
Continuous-Flow and Continuous-Exchange
Cell-Free Translation Systems
CHAPTER 8
Continuous-Flow and Continuous-Exchange
Cell-Free Translation Systems and Reactors ................ 91
VLADIMIR A. SHIROKOV, PETER N. SIMONENKO,
SERGEY V. BIRYUKOV and ALEXANDER S. SPIRIN
CHAPTER 9
Highly Productive Plant Continuous Cell-Free System ....... 109
KAIRAT MAD IN, TATSUYA SAWASAKI and YAETA ENDO
CHAPTER 10
Matrix Reactor: A New Scalable Reactor Principle
for Cell-Free Protein Expression ......................... 121
BERND BUCHBERGER, WOLFGANG MUTTER, ALBERT ReIDER
Part IV
Protein Folding in Cell-Free Translation System
CHAPTER 11
Co-Translational Protein Folding in Prokaryotic
and Eukaryotic Cell-Free Translation Systems. . .. . ... . . .. .. 131
VYACHESLAV A. KOLB, AlGAR KOMMER
and ALEXANDER S. SPIRIN
CHAPTER 12
Expression of an Aggregation-Prone Protein
in the RTS 500 System ................................... 141
JEAN-MICHEL BETTON
CHAPTER 13
Expression of Soluble Murine Endostatin
with the RTS System ................................... 149
STACEY TRAVIGLIA and MONIQUE VAN HOEK
Contents gn
CHAPTER 14
Cell-Free Expression of Soluble Human Erythropoietin 157
CORDULA NEMETZ, STEPHANIE WESSNER, SIMONE KRUPKA,
MANFRED WATZELE and WOLFGANG MUTTER
CHAPTER 15
Cell-Free Expression of a 127 kDa Protein:
The Catalytic Subunit of Human Telomerase ............... 165
ROBIN STEIGERWALD, CORDULA NEMETZ,
BARBEL WALCKHOFF and THOMAS EMRICH
CHAPTER 16
The Expression of Disulfide Bonded Proteins
in Cell-Free Protein Expression .•..•.•.••..•.•.•..•........ 175
ERHARD FERNHOLZ, KATRIN ZAISS, HOSEYIN BESIR
and WOLFGANG MUTTER
Part V
Special Topics and Short Communications
CHAPTER 17
Structural and Functional Compensation
by Proteins for the RNA Deficit
of Animal Mitochondrial Translation Systems ............. 183
TSUTOMU SUZUKI, TAKASHI OHTSUKI,
YOH-ICHI WATANABE, MAKI TERASAKI,
TAKAO HANADA and KIMITSUNA WATANABE
CHAPTER 18
Proficient Target Selection in Structural Genomics by In Vitro
Protein Expression on Gateway Recombination Plasmids 197
RENAUD VINCENTELLI, CHANTAL ABERGEL,
CELINE DEREGNAUCOURT, JEAN-MICHEL CLAVERIE
and VINCENT MONCHOIS
CHAPTER 19
High-Level Cell-Free Protein Expression
from PCR-Generated DNA Templates ..................... 203
THOMAS HOFFMANN, CORDULA NEMETZ,
REGINA SCHWEIZER, WOLFGANG MUTTER
and MANFRED WATZELE
mID
Contents
CHAPTER 20
Optimization of the Translation Initiation Region
of Prokaryotic Expression Vectors: High Yield In Vitro
Protein Expression and mRNA Folding ................... 211
ANDREA GRAENTZDOERFFER, MANFRED WATZELE,
BERND BUCHBERGER, SABINE WIZEMANN,
THOMAS METZLER, WOLFGANG MUTTER
and CORDULA NEMETZ
CHAPTER 21
Selective Labeling of Proteins in the RTS 500 System ........ 219
JEAN-MICHEL BETTON, NICOLAE PALIBRODA,
ABDELKADER NAMANE, THOMAS METZLER
and OCTAVIAN BARZU
CHAPTER 22
In Vitro Protein Production for Structure Determination
with the RTS System ................................... 227
Ho S. CHO, JEFFREY G. PELTON, WEIRU WANG,
HISAO YOKOTA and DAVID E. WEMMER
CHAPTER 23
Sequence Specific Biotinylation and Purification
of Proteins Expressed in the RTS 500 System ............... 235
MICHAEL SCHRAML, DOROTHEE AMBROSIUS,
JAN STRACKE and MARTIN LANZENDORFER
CHAPTER 21
Synthesis of the His 6-Tagged Recombinant
Protein APPC99-His and His-PSI Using RTS 500 ............ 247
MIYUKI MURAYAMA, XIAOYAN SUN and AKIHIKO TAKASHIMA
PART I
Introduction
CHAPTER 1
Cell-Free Protein Synthesis 3
ALEXANDER S. SPIRIN
CHAPTER 1
Cell-Free Protein Synthesis 1
ALEXANDER S. SPIRIN*
Abbreviations
NTP nucleoside triphosphates
ARSes aminoacyl-tRNA synthetases
PEP phosphoenol pyruvate
CP creatine phosphate
AcP acetyl phosphate
PK pyruvate kinase
CK creatine kinase
AcK acetyl kinase
ME mercaptoethanol
DTT dithiotreitol
DHFR dihydrofolate reductase
CAT chloramphenicol acetyltransferase
GFP green fluorescent protein
IL-2 and IL-6 interleukin-2 and interleukin-6
TMV tobacco mosaic virus
Introduction: Prehistory of Cell-Free Translation Systems
As early as the beginning of the 1950s, several groups independently demonstrat
ed that protein synthesis does not require the integrity of the cell and can contin
ue after cell disruption. Thus, disrupted cells or their isolated fractions were
reported to be capable of synthesizing proteins (Borsook 1950; Winnick 1950a,
1950b; Siekevitz and Zamecnik 1951; Siekevitz 1952; Peterson and Greenberg
1952; Khesin 1953; Gale and Folkes 1954). In the meantime, ribonucleoprotein
particles were observed and identified in cells (Palade 1955) and then isolated
from cells and studied with respect to their physicochemical properties (Chao
and Schachman 1956; Ts'o et al. 1956; Peterman and Hamilton 1957; Tissieres and
Watson 1958; Tissieres et al. 1959; see also papers in Roberts 1958). The protein-
* Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino,
Moscow Region, Russia
Tel.!Fax: [phone], e-mail: [email protected]