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CARBON SEQUESTRATION UNDER LONG TERM FERTILIZER APPLICATION IN RICE, MAIZE AND GROUNDNUT BASED CROPPING SYSTEMS IN DIFFERENT AGRO CLIMATIC ZONES P.V. GEETHA SIREESHA M.Sc. (Ag.) DOCTOR OF PHILOSOPHY IN AGRICULTURE (SOIL SCIENCE) 2016 CARBON SEQUESTRATION UNDER LONG TERM FERTILIZER APPLICATION IN RICE, MAIZE AND GROUNDNUT BASED CROPPING SYSTEMS IN DIFFERENT AGRO CLIMATIC ZONES BY P.V. GEETHA SIREESHA M.Sc., (Ag.) THESIS SUBMITTED TO THE PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF DOCTOR OF PHILOSOPHY IN AGRICULTURE (SOIL SCIENCE) CHAIRPERSON: Dr. G.PADMAJA DEPARTMENT OF SOIL SCIENCE AND AGRICULTURAL CHEMISTRY COLLEGE OF AGRICULTURE RAJENDRANAGAR, HYDERABAD – 500 030 PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY 2016 DECLARATION I, P.V. GEETHA SIREESHA, hereby declare that the thesis entitled “CARBON SEQUESTRATION UNDER LONG TERM FERTILIZER APPLICATION IN RICE, MAIZE AND GROUNDNUT BASED CROPPING SYSTEMS IN DIFFERENT AGRO CLIMATIC ZONES” submitted to the Professor Jayashankar Telangana State Agricultural University, Hyderabad for the award of degree of Doctor of Philosophy in Agriculture is the result of original research work done by me. I also declare that no material contained in this thesis has been published earlier in any manner. Place : (P.V. GEETHA SIREESHA) I.D. No. RAD/2012-25 Date : CERTIFICATE This is to certify that the thesis entitled “Carbon Sequestration Under Long Term Fertilizer Application in Rice, Maize and Groundnut Based Cropping Systems in Different Agro Climatic Zones” submitted in partial fulfillment of the requirements for the award of degree of Doctor of Philosophy in Agriculture to the Professor Jayashankar Telangana State Agricultural University, Hyderabad, is a record of the bonafide research work carried out by Mrs. P.V. Geetha Sireesha under our guidance and supervision. No part of the thesis has been submitted by the student for the award of any other degree or diploma. The published part has been fully acknowledged. All assistance and help received during the course of the investigations have been duly acknowledged by the author of the thesis. Chairperson of the Advisory committee Thesis approved by the Student Advisory Committee: Chairperson : Dr. G. PADMAJA ______________ Professor Dept. Soil Science & Agril. Chemistry College of Agriculture, PJTSAU Rajendranagar, Hyd– 500 030. Member : Dr. P. CHANDRA SEKHAR RAO ______________ Dean of Agriculture & Dean of P.G. Studies, PJTSAU Rajendranagar, Hyd– 500 030. Member : Dr. T. RAMESH ______________ Professor Dept. of Crop Physiology College of Agriculture, PJTSAU Rajendranagar, Hyd– 500 030. Member : Dr. R. SUBHASH REDDY ______________ Dean of Student Affairs, PJTSAU Rajendranagar, Hyd– 500 030. Member : Dr. M. VENKATA RAMANA ______________ Professor & Head AICRP on Integrated Farming System Rajendranagar, Hyd– 500 030. External : Dr. V.R. RAMAKRISHNA PARAMA ______________ Examiner Professor Dept. Soil Science & Agril. Chemistry College of Agriculture, UAS GKVK, Banglore– 560 065. Date of final viva-voce: 19-04-2016 CERTIFICATE Mrs. P.V. Geetha Sireesha has satisfactorily prosecuted the course of research and that the thesis entitled “Carbon Sequestration Under Long Term Fertilizer Application in Rice, Maize and Groundnut Based Cropping Systems in Different Agro Climatic Zones” submitted is the result of original research work and is of sufficiently high standard to warrant its presentation to the examination. I also certify that neither the thesis nor its part thereof has been previously submitted by her for a degree of any University. Date : Dr. G. PADMAJA Chairperson Professor Dept. Soil Science and Agril. Chemistry College of Agriculture, PJTSAU Rajendranagar, Hyd– 500 030. ACKNOWLEDGEMENTS It is by the love and blessings of the Almighty, that I have been able to complete my thesis work successfully and present this piece of work. It cannot be argued with that the most influential person in my entire course of this study has been my major advisor Dr. G. Padmaja, Professor, Department of Soil Science and Agricultural Chemistry, for her encouragement, insightful guidance, and discipline have been indispensable to my growth over these four years. I am also grateful to Dr. P. Chandrasekhar Rao, Dean of PG studies, PJTSAU for being an extraordinary Co-Chairperson who showed me the road and helped to get me started on the path to Ph.D. degree. His enthusiasm, encouragement and faith in me throughout have been extremely helpful. He always knew where to look for the answers to obstacles while leading me to the right source, theory and perspective. I would also like to express my heartfelt, thanks to Dr. P.Venkateswara Rao and Dr. T. Ramesh, Professor, Department of Crop Physiology, members of my advisory committee for their constant encouragement. It’s my pleasure to acknowledge Dr. R. Subhash Reddy, Dean of Student Affairs, PJTSAU, as member of my advisory committee for his constant moral support. I am thankful to Dr. M. Venkata Ramana, Principal Scientist and Head, AICRP on Integrated Farming Systems, Rajendranagar, member of my advisory committee for his help during my research work. I wish to express my sincere gratitude to Dr. K. Jeevan Rao, Professor and University Head, Department of Soil Science and Agricultural Chemistry, for his amicable suggestions, benevolent and noble ideas during my research work. I owe my effusive thanks to other staff members of the Department of Soil Science and Agricultural Chemistry, Dr. G. Jayasree, Dr. V. Sailaja Dr. P. Prabhu Prasidini, Dr. Harish Kumar Sharma, Dr. Ch. Sujani Rao, Dr. M. Srilatha, Dr. M. Rajeshwar Naik, and Dr. T. Anjaiah for their constructive advice and timely help during my research work. I warmly thank Dr. D. Balaguravaiah, Dr. A. Madhavi, Dr. Hussain and Sri M. Jeevaratna Raju for their valuable advice, constructive criticism and extensive discussions during my course work. My sincere thanks to Dr. Triveni, Sreedevi and Neeraja for their support during my experimentation in Microbiology laboratory. I wish to extend my sincere gratitude to Dr. L. Kishan Reddy, ADR (Rtd.), RARS, Jagtial, Dr. M. Vijay Shankar Babu, Scientist, DARS, Ananthapur, Dr. Ch. Sreenivas, Scientist, APRRI, Maruteru and Dr. S. Sridevi, Scientist AICRP on farming systems, Rajendranagar for helping me in collection of soil and plant samples from the long-term fertilizer experiment fields. My heartfelt thanks to Dr. M. Surendra Babu, Principal Scientist & Head, Dr. M. Chandini Patnaik, Senior Scientist AICRP on Micro nutrients for helping me to carry out part of my research work. I will forever be thankful to my former college research advisor, Dr. M.V.S Naidu Associate Professor, Department of Soil Science & Agricultural Chemistry, S.V. Agricultural College, Tirupathi for providing his valuble advice during my course of study. I especially thank my parents Smt. K. Lalithamma and Sri. P.V. Subbarao, and brother P.V. Sri Chakra for their everlasting love, persistent encouragement. Both have instilled many admirable qualities in me and given me a good foundation with which to meet life. They’ve taught me about hard work and self-respect, about persistence and about how to be independent. There are no words to convey my adoration and boundless affection on my beloved husband Mr. O. Vinay Kumar who has been a true and great supporter throughout my progress of research work. I am indebted to my student colleagues Srijaya, Sathish, Kiran, Sharan, Sai kumar, Ravi and Aruna Kumari, Seniors Chaitanya, Kalyani, Kamalakar, Ravi Ramakrishna (SRF) and Juniors Naveen, Madhavi, Prabhavathi, Ranjit, Venugopal, Swetha, Archana, Apoorva, Jessi, Miraj, Naresh and Rajasekhar for their cooperation and help rendered during the study. My heartful gratitude to my beloved friends Jyothi, Manasa, Archana, Kavya, Himaja, Madhuri, Gouthami, Bindu, Usha, Sneha, Rama, Jyosna, Nagendra and Sridhar for their endearment, encouragement, valuable moral support and pleasant cooperation rendered to me during the progress of the work. I extremely thankful to the supporting staff of the Department of Soil Science and Agricultural Chemistry, for their timely support and help rendered during all the period of my study. I am greatfully acknowledge Acharya N.G. Ranga Agricultural University, Government of Andhra Pradesh and Professor Jayashankar Telangana State Agricultural University Government of Telangana for their financial assistance provided in the form of stipend during my Ph.D. programme. I sincerely thank all who helped me directly and indirectly to bring out this humble piece of work. Besides this, several people have knowingly and unknowingly helped me in the successful completion of this project. If I have forgotten anyone, I apologize.  Geetha Sireesha… CONTENTS Chapter No. Title Page No. I INTRODUCTION 1-3 II REVIEW OF LITERATURE 4-34 III MATERIAL AND METHODS 35-62 IV RESULTS AND DISCUSSION 63-206 V SUMMARY AND CONCLUSIONS 207-211 LITERATURE CITED 211-242 APPENDICES 243-249 LIST OF TABLES Table Page Title No. No. 3.1 Methods followed for the analysis of soil sample 53 4.1 Long-term effects of INM on different carbon fractions of the soils 69 after harvest of kharif rice under rice-rice cropping system at Rajendranagar 4.2 Long-term effects of INM on different carbon fractions of the soils 70 after harvest of rabi rice under rice-rice cropping system at Rajendranagar 4.3 Long term effects of INM treatments on carbon stocks and carbon 72 sequestration rate under rice-rice cropping system at Rajendranagar 4.4 Long-term effects of INM on enzyme activities of the soils after 76 harvest of kharif rice under rice-rice cropping system at Rajendranagar 4.5 Long-term effects of INM on enzyme activities of the soils after 77 harvest of rabi rice under rice-rice cropping system at Rajendranagar 4.6 Long-term effects of INM on physico-chemical properties of soil 80 after harvest of rice-rice cropping system at Rajendranagar 4.7 Long-term effects of INM on available N, P and K status (kg ha-1) 82 of the soils after harvest of rice-rice cropping system at Rajendranagar 4.8 Long-term effects of INM on available micronutrient status (mg 84 kg-1) of the soils after harvest of rice – rice cropping system at Rajendranagar 4.9 Long-term effects of INM on total N, P and K contents (%) of the 86 grain after harvest of rice-rice cropping system at Rajendranagar 4.10 Long-term effects of INM on total N, P and K contents (%) of the 87 straw after harvest of rice-rice cropping system at Rajendranagar 4.11 Long-term effects of INM on N, P and K uptake (kg ha-1) by grain 88 after harvest of rice-rice cropping system at Rajendranagar 4.12 Long-term effects of INM on N, P and K uptake (kg ha-1) by straw 89 after harvest of rice-rice cropping system at Rajendranagar 4.13 Long-term effects of INM on grain and straw yields (kg ha-1) after 92 harvest of rice – rice cropping system at Rajendranagar 4.14 Long-term effects of INM on different carbon fractions of the soils 96 under maize-onion cropping system at Rajendranagar 4.15 Long term effects of INM treatments on carbon stocks and carbon 100 sequestration rate under maize-onion cropping system at Rajendranagar 4.16 Long-term effects of INM on enzyme activities of the soils under 104 maize-onion cropping system at Rajendranagar 4.17 Long-term effects of INM on physico-chemical properties of soils 106 after harvest of maize-onion cropping system at Rajendranagar 4.18 Long-term effects of INM on available N, P and K status (kg ha-1) 108 of the soils after harvest of maize-onion cropping system at Rajendranagar Table Page Title No. No. 4.19 Long-term effects of INM on available micronutrient status (mg 110 kg-1) of the soils after harvest of maize-onion cropping system at Rajendranagar 4.20 Long-term effects of INM on total N, P and K contents (%) in 111 maize kernels and onion bulbs after harvest of maize - onion cropping system at Rajendranagar 4.21 Long-term effects of INM on total N, P and K contents (%) in 112 maize (stover) and onion (straw) after harvest of maize-onion cropping system at Rajendranagar 4.22 Long-term effects of INM on N, P and K uptake (kg ha-1) by 114 maize kernels and onion bulbs after harvest of maize - onion cropping system at Rajendranagar 4.23 Long-term effects of INM on N, P and K uptake (kg ha-1) by 115 maize (stover) and onion (straw) after harvest of maize - onion cropping system at Rajendranagar 4.24 Long-term effects of INM on yields (kg ha-1) of maize – onion 117 cropping system at Rajendranagar 4.25 Long-term effects of INM on different carbon fractions of the soils 123 under rice-rice cropping system at Jagtial 4.26 Long term effects of INM treatments on carbon stocks and carbon 125 sequestration rate under rice-rice cropping system at Jagtial 4.27 Long-term effects of INM on enzyme activities of the soils under 129 rice-rice cropping system at Jagtial 4.28 Long-term effects of INM on physico-chemical properties of soil 131 after harvest of rice – rice cropping system at Jagtial 4.29 Long-term effects of INM on available N, P and K status (kg ha-1) 133 of the soils after harvest of rice-rice cropping system at Jagtial 4.30 Long-term effects of INM on available micronutrient status (mg 134 kg-1) of the soils after harvest of rice – rice cropping system at Jagtial 4.31 136 Long-term effects of INM on total N, P and K contents (%) of the grain after harvest of rice-rice cropping system at Jagtial 4.32 Long-term effects of INM on total N, P and K contents (%) of the 137 straw after harvest of rice-rice cropping system at Jagtial 4.33 Long-term effects of INM on N, P and K uptake (kg ha-1) by grain 140 after harvest of rice-rice cropping system at Jagtial 4.34 Long-term effects of INM on N, P and K uptake (kg ha-1) by straw 141 after harvest of rice-rice cropping system at Jagtial 4.35 Long-term effects of INM on grain and straw yields (kg ha-1) after 142 harvest of rice – rice cropping system at Jagtial 4.36 148 Long-term effects of INM on different carbon fractions of the soils under rice-rice cropping system at Maruteru 4.37 Long term effects of INM treatments on carbon stocks and carbon 150 sequestration rate under rice-rice cropping system at Maruteru 4.38 Long-term effects of INM on enzyme activities of the soils under 154 rice-rice cropping system at Maruteru

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University Head, Department of Soil Science and Agricultural Chemistry, and Neeraja for their support during my experimentation in Microbiology I am greatfully acknowledge Acharya N.G. Ranga Agricultural University, If I have forgotten anyone, I .. These factors lead to food insecurity, more.
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