Branched-Chain Amino Acids, Part B

581 Pages·2000·8.27 MB·English

by  John R. Sokatch

Contributors to Volume 324
Article numbers are in parentheses gniwollof the names of .srotubirtnoc
snoitailiffA listed are current.
ZE'EV BARAK (2, 10), Department of Life Sci- western Medical Center, Dallas, Texas
ences, Ben-Gurion University of the Negev, 8309-09357
Beer-Sheva ,50148 Israel MYRA CONWAY (33), Department of Bio-
MICHAEL J. BEACH (25), Centers for Disease chemistry, Wake Forest University School
Control, Atlanta, Georgia, 4273-14303 of Medicine, Winston-Salem, North Caro-
PHILIP R. BECKETT (5), Baylor College of lina 27157
Medicine, Houston, Texas 03077 PAUL A. COSTEAS (43), Karaiskakio Founda-
BARBARA BINZAK (24), Department of Bio- tion, The Cyprus Bone Marrow and Platelet
chemistry and Molecular Biology, Mayo Donor Registry, Nicosia, Cyprus
Clinic and Mayo Foundation, Rochester, DAVID W. CRABB (22), Departments of Medi-
Minnesota 50955 cine, and Biochemistry and Molecular Biol-
KENNETH M. BISCHOFF (25), Food Animal ogy, lndiana University School of Medicine,
Protection Research Laboratory, .U .S De- Indianapolis, lndiana 2215-20264
partment of Agriculture, College Station, NICHOLAS P. CROUCH (32), The Dyson Per-
Texas 54877 tins Laboratory, University of Oxford, Ox-
DANIEL A. BOCHAR (25), The Wistarlnstitute, ford OX1 3QY, United Kingdom
Philadelphia, Pennsylvania 8624-40191 DEAN J. DANr, REr (31, 44), Department of Ge-
JOSEPH M. CALVO (29), Section of Biochemis- netics, Emory University School of Medi-
try, Molecular and Cell Biology, Cornell cine, Atlanta, Georgia 22303
University, Ithaca, New York 35841 BRYANT G. DARNAY (25), UTMDA Cancer
REBECCA M. CHAN (22), Department of Bio- Center, Cytokine Research Section, Hous-
chemistry and Molecular Biology, Indiana ton, Texas 03077
University School of Medicine, Indianapo- JAMES R. DAVIE (18, 19), Department of Bio-
lis, Indiana 2215-20264 chemistry, University of Texas, Southwest-
YUHAI CHI (29), Department of Molecular ern Medical Center, Dallas, Texas -09357
Biology and Genetics, University of Guelph, 8309
Guelph, Ontario N1G 2W1, Canada J. RICHARD DICKINSON (9, 36), Cardiff School
JEFFREY M. CHINSKY (43), Department of Pe- of Biosciences, Cardiff University, Cardiff
diatrics, Johns Hopkins University School CFIO 3TL, United Kingdom
of Medicine, Baltimore, Maryland 21287 REHPOTSIRHC B. DOERING (44), Department
DAVID M. CHIPMAN (2, 10), Department of of Genetics, Emory University School of
Life Sciences, Ben-Gurion University of the Medicine, Atlanta, Georgia 22303
Negev, BeeroSheva 84105, Israel TSIONA ELKAYAM (10), Department of Life
DAVID T. CHUANG (18, 19, 38, 45), Depart- Sciences, Ben-Gurion University of the
ment of Biochemistry, University of Texas, Negev, Beer-Sheva 84105, Israel
Southwestern Medical Center, Dallas, Texas SABINE EPELBAUM (2), Central Research and
8309-09357 Development Department, E.I. duPont de
JACINTA L. CHUANO (18, 19, 38), Department Nemours and Company, Wilmington, Dela-
of Biochemistry, University of Texas, South- ware 3710-08891
xi
xii CONTRIBUTORS TO VOLUME 324
ABDUL FAUQ (24), Organic Synthesis Core AJITAM HEDL (25), Department of Biochemis-
Facility, Mayo Clinic and Mayo Founda- try, Purdue University, West Lafayette, Indi-
tion, Jacksonville, Florida 32224 ana 3511-70974
HAROVED GREBDEIRF (29), Department of NYRHTAK L. HESTER (14), Dunlop Codding
Molecular Genetics and Biotechnology, The and Rogers, Oklahoma City, Oklahoma
Hebrew University, Faculty of Medicine, Je- 41147
rusalem ,02119 Israel GNUOY DOS HONG (41), Department of -DiB
JoN A. NESEIRF (25), Department of Biochem- chemistry, School of Medicine and Biomed-
istry and Molecular Biology, Illinois State ical Sciences, State University of New York,
University, Normal, Illinois 0014-09716 Buffalo, New York 14214
OKEGIHS OTOMIJUF ATAKAS (35), Laboratory TOSHIMrrsu HOSHINO (12, 13), Mitsubishi
of Nutritional Chemistry, Faculty of Nutri- Kasei Institute of Life Sciences, Tokyo 194-
tion, Kobe Gakuin University, Kobe 651- ,1158 Japan
,0812 Japan BOLl HUANG (23), Department of Biochemis-
IKUYIHSOT OAKUF (40), Department of Pedi- try and Molecular Biology, Indiana Univer-
atrics, Gifu School of Medicine, Gifu 500- sity School of Medicine, Indianapolis, Indi-
,6708 Japan ana 2215-20264
K. MICHAEL NOSBIG (40), Departments of NAIUHS-IY HUANG (45), Department of Bio-
Molecular and Medical Genetics, and Pedi- chemistry, University of Texas Southwest-
atrics, and Biochemical Genetics Labora- ern Medical Center, Dallas, Texas -09357
tory, Oregon Health Sciences University, 8309
Portland, Oregon 10279 NASUS HtrrSON (33), Department of Biochem-
JOHN F. GILL (25), Department of Protein istry, Wake Forest University School of
Chemistry, Boehringer-Mannheim, India- Medicine, Winston-Salem, North Carolina
napolis, Indiana 05264 75172
GARY W. NlWDOOG (21), Division of Car- ASERET ONEUB-AITIOGAGAIRRUTI (32), The
diology, Department of Internal Medicine, Dyson Perrins Laboratory, University of
The University of Texas Houston Medical Oxford, Oxford OX1 3QY, United
School, Houston, Texas 03077 Kingdom
NIWDE T. HARPER (8), Department of Bio- KRAM T. NOSNHOJ (42), Department of Ge-
chemistry and Molecular Biology, Indiana netics, Case Western Reserve University,
University School of Medicine, Indianapo- Cleveland, Ohio 60144
lis, Indiana 2215-20264 ADNAUJAUT KCRATS-NADROJ (25), Depart-
TREBOR A. HARRIS (6, ,71 20, 21, 22, 23), ment of Chemistry, Xavier University of
Department of Biochemistry and Molecular New Orleans, New Orleans, Louisiana
Biology, Indiana University School of Med- 52107
icine, Indianapolis, Indiana 2215-20264 IKUYORIH AMAYIMAGAK (11), Department
NHOJ W. SEWAH (8, 17, 20, 22, 23), Depart- of Biochemistry, Osaka Medical College,
ment of Biochemistry and Molecular Biol- Takatsuki ,6868-965 Japan
ogy, Indiana University School of Medicine, AILATAN Y. ILIVHSIDEK (21), Department of
Indianapolis, Indiana 2215-20264 Molecular Biology and Biochemistry,
IKUYEDIH IHSAYAH (11), Department of -DiB School of Biological Sciences, University of
chemistry, Osaka Medical College, Takat- Missouri-Kansas City, Kansas City, Mis-
suki ,6868-965 Japan souri 64110
OKOY IKASAWI-IHSAYAH (28), Department of RETEP J. YLLENNEK (25), Department of -DiB
Molecular Biology, Tokyo University of chemistry, Virginia Polytechnic and State
Pharmacy and Life Science, Tokyo 192- University, Blacksburg, Virginia 24061-
,2930 Japan 8030
CONTRIBUTORS TO VOLUME 324 xiii
SALGUOD S. KERR (41), Department of Pedi- GRAter A. LLEHCTIM (40), Service de Gene-
atrics and Center for Inherited Disorders of tique Medicale, Hopital Sainte-Justine,
Energy Metabolism, Case Western Reserve Montreal, Quebec H3T 1C5, Canada
University, Cleveland, Ohio 44106
YRNEH M. OKROIZIM (15, 16), Biochemistry
AIZRAM GALLI ELNEIK (7), Department of Department, Medical College of Wisconsin,
Experimental and Environmental Medicine Milwaukee, Wisconsin 53226
and Medical Biotechnology, University of
DILAW-LA A. NESHOM (24), Department of
Milano-Bicocca, Milan, Italy
Medical Genetics, Mayo Clinic and Mayo
GNOD LUY MIK (25), Department of Bio-
Foundation, Rochester, Minnesota 55905
chemistry, Purdue University, West Lafay-
ette, Indiana 3511-70974 ORAT IMAKARUM (23), Department o f B iosci-
ence, Nagoya Institute of Technology, Na-
ALUYG LAPSIK (34), Institute of Biochemistry,
goya ,5558-664 Japan
University Medical School of Pecs, 7624
Pecs, Hungary AYOAN NAKAI (6, 23), Research Center of
IMUR IHSAYABOK (6), Department of Bio- Health, Physical Fitness and Sports, Nagoya
chemistry and Molecular Biology, Indiana University, Nagoya ,1068-464 Japan
University School of Medicine, Indianapo- YHTRAVARKAHC NAHMISARAN (15, 16), Lilly
lis, Indiana 2215-20264 Research Laboratories, Indianapolis, Indi-
EEuI-I-GNEM EEL (32), The Dyson Perrins ana 46285
Laboratory, University of Oxford, Oxford LISAB J. UALOKIN (26), Department of Bio-
OX1 3QY, United Kingdom
chemistry, Biophysics and Molecular Biol-
DNALOR LIEL (34), Institut flit Zytobiologie ogy, Iowa State University, Ames, Iowa
und Zytopathologie, Philipps-Universittit, 11005
35033 Marburg, Germany
ORIAT AMIHSO (28), Department of Molecu-
EHNIJ OUL (14), Department of Biochemistry lar Biology, Tokyo University of Pharmacy
and Molecular Biology, University of Okla- and Life Science, Tokyo ,2930-291 Japan
homa Health Sciences Center, Oklahoma
DNAHCLUM S. PATEL (41, 42), Department
City, Oklahoma 09137
of Biochemistry, School of Medicine and
NILOC H. NONvnKcAM (32), The Dyson Per-
Biomedical Sciences, State University of
rins Laboratory, University of Oxford, Ox-
New York, Buffalo, New York 41241
ford OX1 3QY, United Kingdom
HPLAR NOTXAP (22), Laboratory of Metabolic
IEUPANUK Z. NAHDUSUHDAM (30), Central
Disorders, Departments of Anatomy, Physi-
Arkansas Veterans Healthcare System, Pa-
ology and Pharmacology, College of Veteri-
thology and Laboratory Medicine, Little
nary Medicine, Auburn University, Auburn,
Rock, Arkansas 50227
Alabama 4045-94863
OlVLUF MAGNI (7), IRCCS San Raffaele,
Mass Spectrometry Unit, Milan, Italy EEIRIK M. PoPOV (6, ,71 20, 21), Department
of Molecular Biology and Biochemistry,
LAVRO A. RZMAM (1), The Mass Spectrome-
School of Biological Sciences, University of
try Unit, McGill University, Montreal, Que-
Missouri-Kansas City, Kansas City, Mis-
bec H3A 1A3, Canada
souri 64110
IHCIOK ADUSTAM (35), Laboratory of Nutri-
tional Chemistry, Faculty of Nutrition, Kobe ANNIROC ENORP (34), Institut f~r Zytobiolo-
Gakuin University, Kobe ,0812-156 Japan gie und Zytopathologie, Philipps-Universi-
tat, 35033 Marburg, Germany
ANEWOR G. SWEHTTAM (29), Biophysics Re-
search Division and Department of Biologi- ENILEUQCAJ R. STREBOR (16), Chemistry De-
cal Chemistry, University of Michigan, Ann partment, De Pauw University, Greencastle,
Arbor, Michigan 5501-90184 Indiana 53164
xiv CONTRIBUTORS TO VOLUME 324
ROTCIV W. LLEWDOR (25), Department of logia Molecular, Universidad Autonoma de
Biochemistry, Purdue University, West La- Madrid, 28049 Madrid, Spain
fayette, Indiana 3511-70974 OKIHIHSOY URATANI (12, 13), Mitsubishi
SELRAHC R. EOR (39), Institute of Metabolic Kasei Institute of Life Sciences, Tokyo -491
Disease, Baylor University Medical Center, ,1158 Japan
Dallas, Texas 62257 YRREJ YELKCOV (24), Department of Medical
ENAID S. EOR (39), Institute of Metabolic Dis- Genetics, Mayo Clinic and Mayo Founda-
ease, Baylor University Medical Center, tion, Rochester, Minnesota 55905
Dallas, Texas 62257 MARIA YKSNEMZAYV (10), Department of
LUAP M. FFARGUOR (22), Naples, Florida Life Sciences, Ben-Gurion University of the
20143 Negev, Beer-Sheva 84105 Israel
ILUY GNAW (25), Ann Arbor, Michigan 50184
RETEP TDLAWEDAHCS (3, 4), Deutsches Dia-
betes Forschungsinstitut, Klinische Bio- NAJ DRALLIW (24), Department of Medical
chemie, D-40225 Diisseldorf, Germany Genetics, Mayo Clinic and Mayo Founda-
tion, Rochester, Minnesota 55905
URAHIHSOY ARUMOMIHS (6, ,71 20, 23), De-
partment of Bioscience, Nagoya Institute of EVE NIKRYS ELETRUW (26), Department of
Botany, Iowa State University, Ames,
Technology, Nagoya ,5558-664 Japan
Iowa 11005
NHOJ R. HCTAKOS (14, 30), Department of
R. XAM NNYW (18, 19), Department of Inter-
Biochemistry and Molecular Biology, Uni-
laH Medicine, University of Texas, South-
versity of Oklahoma Health Sciences Cen-
western Medical Center, Dallas, Texas
ter, Oklahoma City, Oklahoma 09137
9888-09357
JIu-LI SON~ (18), Department of Biochemis-
GNEHS-NISH GNAY (42), Department of Bio-
try, University of Texas, Southwestern Med-
chemistry, School of Medicine and Biomed-
ical Center, Dallas, Texas 8309-09357
ical Sciences, State University of New York,
AYANAN IKAMAT (35), Laboratory of Nutri- Buffalo, New York 14214
tional Chemistry, Faculty of Nutrition, Kobe
Yu ZHAO (20), Bayer Corporation, Clayton,
Gakuin University, Kobe ,0812-156 Japan
North Carolina 27520
SAMOHT W. TUART (37), Department of Bio- RENIAR REHCOZ (27), Max-Volmes-Institut
chemistry and Biophysics, University of
far Biophysikalische Chemie, Fachgebiet
North Carolina School of Medicine, Chapel
Biochemie und Moleculare Biologie, Tech-
Hill, North Carolina 0627-99572
nische Universitiit Berlin, D-10587 Berlin,
ANELADGAM ETRAOU (40), Department Bio- Germany
Preface
Branched-chain amino acids were first covered in Methods in Enzymol-
ogy Volume XVII: Metabolism of Amino Acids and Amines, Part A, edited
by H. W. Tabor and C. W. Tabor. It included a section on biosynthetic
pathways for branched-chain amino acids in prokaryotes, which was the
state of the art at that time. In 1988, we edited Methods in Enzymology
Volume 166, Branched-Chain Amino Acids, which included sections on the
purification of several enzymes of branched-chain amino acid metabolism,
specific enzyme assays, and the quantification of metabolites in branched-
chain amino acid pathways. In the past twelve years, recombinant DNA
technology has had a major impact on the understanding of the structure,
function, regulation, and expression of enzymes of branched-chain amino
acid metabolism, including genetic defects in these pathways which afflict
humans.
This volume features the purification and characterization of several
enzymes of branched-chain amino acid catabolism that were unknown when
Volume 166 was published. Improvements on earlier methods for purifica-
tion of key regulatory enzymes, the assay of various enzymes in purified
form and in tissue extracts, and the determination of important metabolites
of branched-chain amino acid pathways are also given. The most important
feature of the volume, however, is the information resulting from the appli-
cation of recombinant DNA methodology to the study of the enzymes of
branched-chain amino acid metabolism. This includes sections on cloning
cDNAs encoding many of the branched-chain amino acid enzymes, expres-
sion and characterization of several of these enzymes as recombinant pro-
teins, and the identification of mutations responsible for genetic defects in
enzymes of branched-chain amino acid catabolism. This volume will be of
use to investigators interested in the structure, function, regulation, and
expression of enzymes of branched-chain amino acid metabolism as well
as to investigators who study genetic diseases caused by defects in the
enzymes of catabolic pathways for these amino acids.
We would like to express our appreciation to the authors whose contri-
butions made this volume possible.
TREBOR A. SIRRAH
JOHN R. SOKATCH
VX
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited yb YENDIS P, KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV II. Preparation and Assay of Enzymes
Edited yb YENDIS P, KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
detidE by YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV IV. Special Techniques for the Enzymologist
detidE by YENDIS P, KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV V. Preparation and Assay of Enzymes
Edited yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
detidE yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VII. Cumulative Subject Index
Edited yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VIII. Complex Carbohydrates
detidE by HTEBAZILE F. DLEFUEN DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb SILLIW A. DOOW
EMULOV X. Oxidation and Phosphorylation
detidE by DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
detidE yb .C H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and B)
detidE yb ECNERWAL NAMSSORG DNA KIVlE EVADLOM
EMULOV XIII. Citric Acid Cycle
detidE by J. M. NIETSNEWOL
EMULOV XIV. Lipids
detidE by J. M. NIETSNECVOL
EMULOV XV. Steroids and Terpenoids
detidE by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
detidE yb HTENNEK NITSUK
iivx
xviii
METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and )B
Edited by TREBREH ROBAT DNA CELIA ETIHW ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, ,B dna )C
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVlE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part )B
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by .C H. W. Hips DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KiVtE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited by NHOJ M. NI~aSNEWOL
SDOHTEM NI YGOLOMYZNE xix
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by TREB W. YELLAM'O DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by NHOJ H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. EEFFOH DNA YRAM NELLE SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
XX METHODS IN ENZYMOLOGY
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited yb YENDIS FLEISCHER AND LESTER PACKER
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. DELUCA
EMULOV LVIII. Cell Culture
Edited by WILLIAM B. JAKOBY AND IRA PASTAN
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVIE MOLDAVE AND LAWRENCE GROSSMAN
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. WRIGHT
EMULOV .36 Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by DANIEL L. HCIRUP
EMULOV .46 Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and Com-
plex Enzyme Systems)
Edited by DANIEL L. HCIRUP
EMULOV .56 Nucleic Acids (Part I)
Edited by LAWRENCE GROSSMAN AND KIVtE MOLDAVE
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited by DONALD B. McCoRMICK AND LEMUEL D. WRIGHT
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DONALD B. McCoRMICK AND LEMUEL D. WRIGHT
EMULOV .86 Recombinant DNA
Edited by YAR Wu
EMULOV .96 Photosynthesis and Nitrogen Fixation (Part C)
Edited by YNOHTNA NAS ORTEIP
EMULOV .07 Immunochemical Techniques (Part A)
Edited by HELEN VAN VUNAKIS AND JOHN J. LANGONE
EMULOV .17 Lipids (Part C)
Edited by JoHN M. NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by JoHN M. NIETSNEWOL
SDOHTEM NI YGOLOMYZNE xxi
EMULOV .37 Immunochemical Techniques (Part B)
Edited by NHOJ J. LANGO~ DNA HELEN VAN SIKANUV
EMULOV .47 Immunochemical Techniques (Part C)
Edited yb NHOJ J. ENOGNAL DNA HELEN VAN SIKANUV
EMULOV .57 Cumulative Subject Index Volumes XXXI, XXXII, XXXIV-LX
detidE yb EDWARD A. DENNIS DNA MARTHA G. DENNIS
EMULOV .67 Hemoglobins
detidE yb ERALDO ANTONINI, LUIGI ROSSI-BERNARDI, DNA EMILIA ENOCNAIHC
EMULOV .77 Detoxication and Drug Metabolism
Edited yb WILLIAM B. YBOKAJ
EMULOV .87 Interferons (Part A)
detidE yb YENDIS AKTSEP
EMULOV .97 Interferons (Part B)
Edited yb YENDIS AKTSEP
EMULOV .08 Proteolytic Enzymes (Part C)
detidE by OLZSAL DNAROL
EMULOV .18 Biomembranes (Part H: Visual Pigments and Purple Mem-
branes, I)
Edited yb RETSEL REKCAP
EMULOV .28 Structural and Contractile Proteins (Part A: Extracellular Matrix)
Edited by NOEL W. MAHGNINNUC DNA DIXIE W. NESKIREDERF
EMULOV .38 Complex Carbohydrates (Part D)
detidE by ROTCIV GRUBSNIG
EMULOV .48 Immunochemical Techniques (Part D: Selected Immunoassays)
Edited yb JoHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV .58 Structural and Contractile Proteins (Part B: The Contractile Appara-
tus and the Cytoskeleton)
detidE yb DIXIE W. NESKIREDERF DNA LEON W. MAHGNINNUC
EMULOV .68 Prostaglandins and Arachidonate Metabolites
Edited by WILLIAM E. M. LANDS DNA WILLIAM L. HTIMS
EMULOV .78 Enzyme Kinetics and Mechanism (Part C: Intermediates, Stereo-
chemistry, and Rate Studies)
detidE by LEINAD L. HCIRUP
EMULOV .88 Biomembranes (Part I: Visual Pigments and Purple Mem-
branes, II)
Edited yb RETSEL REKCAP
EMULOV .98 Carbohydrate Metabolism (Part D)
detidE by WILLIS A. WOOD
EMULOV .09 Carbohydrate Metabolism (Part E)
detidE by WILLIS A. WOOD