Blood Cells, Rheology, and Aging

228 Pages·1988·5.244 MB·English

by  D. Danon, A. Knyszynski, L. Hayflick (auth.), Professor Dr. med. Dieter Platt (eds.)

Blood Cells,
Rheology,
and Aging
Edited by Dieter Platt
With 67 Figures and 47 Tables
Springer-Verlag
Berlin Heidelberg New York
London Paris Tokyo
Professor Dr. med. DIETER PLATT
Direktor des Instituts fUr Gerontologie
der Universitat Erlangen-Niirnberg
und der 2. Medizinischen Klinik
Klinikum Niirnberg, FlurstraBe 17
D-8500 Niirnberg, FRG
ISBN-13: 978-3-642-71792-5 e-ISBN-13: 978-3-642-71790-1
DOl: 10.1007/978-3-642-71790-1
Library of Congress Cataloging-in-Publication Data. Blood cells, rheology and aging. Based
on the third Erlangen-Nuremberg symposium held in 1986. Includes index. 1. Erythrocytes ~
Aging ~ Congresses. 2. Lymphocytes ~ Aging ~ Congresses. 3. Blood ~ Viscosity ~ Age factors ~
Congresses. I. Platt, Dieter. [DNLM: 1. Aging ~ congresses. 2. Blood Cells ~ congresses. 3. Blood
Circulation ~ congresses. 4. Erythrocyte Aging ~ congresses. WH 150 B6542 1986) QP96.B54
1988 599:0113 87-28881
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© Springer-Verlag Berlin Heidelberg 1988
Softcover reprint of the hardcover 1st edition 1988
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2122/3130-543210
List of Contributors
E. BEREGI, Prof. Dr., Gerontology Center Med. University,
1085 Somogyi Bela U 33, Budapest, Hungary
D. E. BROOKS, Prof. Dr., University of British Columbia, Department
of Pathology, 2075 Westbrook Place, Vancouver, British Columbia,
Canada V6T1 W5
G. CASALE, Prof. Dr., Istituto de Gerontologia e Geriatria,
Universita degli Studi di Pavia, Piazza Borromeo n. 2, 27100 Pavia,
Italy
M.-P. CHANG, Prof. Dr., Geriatric Center, VA Wadsworth Hospital
Center, 691-11 GA VA Wadsworth Hospital, Wilshire and Sawtelle
BLVDS, Los Angeles, CA 90073, USA
D. DANON, Prof. Dr., Bella S. & Irving E. Meller, Center for Biology of
Aging, The Weizmann Institute, Rehovot, Israel
D. DRENCKHAHN, Prof. Dr., Institut fUr Anatomie und Zellbiologie der
UniversWit Marburg, 3550 Marburg, FRG
D. GERSHON, Prof. Dr., Technion Institute, Technion City, Haifa,
32000, Israel
H. GERSHON, Prof. Dr., Technion Faculty of Medicine, Dept.
Immunology, 12 Aliah St., Haifa, 32000, Israel
G. A. GLASS, Prof. Dr., Technion Institute, Technion City, Haifa,
32000, Israel
K. HAGER, Dr., 2. Med. Klinik, Klinikum Niirnberg, 8500 Niirnberg,
FRG
L. HAYFLICK, Prof. Dr., Center for Gerontological Studies, University
of Florida, Gainesville, USA
H. HECKERS, Prof. Dr., Zentrum fUr Innere Medizin der Universitat
GieBen, 6300 GieBen, FRG
I. HERMANN, Prof. Dr., 2. Med. Klinik, Klinikum Niirnberg,
8500 Niirnberg, FRG
F. JUNG, Dr., Abt. fUr Klinische Haemostaseologie und
Transfusionsmedizin, Universitatskliniken des Saarlandes,
6650 Homburg/Saar, FRG
VI List of Contributors
N. KHANSARI, Prof. Dr., Department of Microbiology, North Dakota
State University, Fargo, North Dakota, 58105, USA
H. KIESEWETIER, Prof. Dr. Dr., Abt. fUr Klinische Haemostaseologie
und Transfusionsmedizin, Universitiitskliniken des Saarlandes,
6650 Homburg/Saar, FRG
N. KINOHARA, Prof. Dr., Geriatric Center, VA Wadsworth Hospital
Center, 691-11 GA VA Wadsworth Hospital, Wilshire and Sawtelle
BLVDS, Los Angeles, CA 90073, USA
A. KNYSZYNSKI, Prof. Dr., Bella S. & Irving E. Meller, Center for
Biology of Aging, The Weizmann Institute, Rehovot, Israel
N. KORBER, Dr., Abteilung Neurologie der Medizinischen Fakultiit der
RWTH Aachen, Pauwelsstrasse, 5100 Aachen, FRG
O. LINDERKAMP, Prof. Dr., University of Heidelberg, 6900 Heidelberg,
FRG
T. MAKINODAN, Prof. Dr., Director Geriatric Center, VA Wadsworth
Hospital Center, 691-11 GA VA Wadsworth Hospital, Wilshire and
Sawtelle BLV DS, Los Angeles, CA 90073, USA
H. J. MEISELMAN, Prof. Dr.,. Department of Physiology, USC School
of Medicine, 2025 Zonal Avenue, Los Angeles, CA 90033, USA
G. B. NASH, Dr., St. George's Hospital Medical School, London, UK
P. DE NICOLA, Prof. Dr., Istituto de Gerontologia e Geriatria,
Universita degli Studi di Pavia, Piazza Borromeo n.2, 27100 Pavia,
Italy
H. P. NUTTGENS, Dr., Abt. fUr Klinische Haemostaseologie und
Transfusionsmedizin, Universitiitskliniken des Saarlandes,
6650 Homburg/Saar, FRG
J. PALEK, Prof. Dr., Haematology Research Laboratory,
St. Elizabeths Hospital, 736 Cambridge Street, Boston,
Massachusetts, 02135, USA
C. PFAFFEROTH, Dr., 1. Medizinische Klinik rechts der Isar,
Technische Universitiit Miinchen, Ismaninger Str. 22,
8000 Miinchen 80, FRG
D. PLATT, Prof. Dr., Institut fUr Gerontologie der Universitiit
Erlangen-Niimberg und 2. Med. Klinik, Klinikum Niimberg,
8500 Niimberg, FRG
W. RIECK, Dr., Institut fUr Gerontologie der Universitiit Erlangen
Niimberg, 8500 Niimberg, FRG
E. B. RINGELSTEIN, Prof. Dr., Abteilung Neurologie der Medizinischen
Fakultiit der RWTH Aachen, Pauwelsstrasse, 5100 Aachen, FRG
List of Contributors VII
G. S. ROTH, Prof. Dr., Gerontological Research Center, National
Institute of Aging, Baltimore, MD 21224, USA
R. SCHNEIDER, Dr., Abteilung fUr Neurologie der Medizinischen
Fakultat der RWTH Aachen, Pauwelsstrasse, 5100 Aachen, FRG
T. VOMEL, Priv.-Doz. Dr., Institut fUr Gerontologie der Universitat
Erlangen-Niirnberg, 8500 Niirnberg, FRG
TH. B. WAHLBERG, Prof. Dr., Department of Blood Coagulation
Disorders, Karolinska Hospital, Stockholm, Sweden
R. L. WALFORD, Prof. Dr., Department of Pathology, UCLA Medical
School, Los Angeles, CA 90024, USA
M. E. WEKSLER, Prof. Dr., Cornell University, Medical College,
1300 York Avenue, New York, N.Y. 10021, USA
S. WOLF, Dr., Abteilung Ophthalmologie der Rheinisch-Westfalischen
Technischen Universitat, 5100 Aachen, FRG
Preface
In the third Erlangen-Nuremberg symposium on experimental geronto
logy we discussed the effect of aging on blood cells and rheology. Most
studies with red cells have been performed on humans, rats, and rabbits.
All these erythrocytes have no nucleus; the cells are therefore a model in
which new synthesis of proteins is impossible. All the processes are age
dependent except for exchange with the plasma. Investigations of red
cells have attracted attention because of the latter's availability in large
quantities from biochemists, physiologists, immunologists, and micro
scopists. We now have a relative abundance of data on the biochemical
and immunologic composition as well as on the ultrastructure of the
cells.
The second topic under discussion at the symposium was the immune
system and cell receptors. One of the most apparent features of aging of
the organism is progressive dysregulation of the immune system. As a
result, the incidence of infection, cancer, and autoimmune and immune
complex diseases increases. While we have a lot of new data on the
function and differentiation of lymphoid cells, we have little informa
tion on lymphocyte receptors during the aging process. Rheologic
studies reflect changes in red cell deformability and blood coagulation.
The erythrocyte deformability influences blood flow in all parts of the
circulation. The fluidity of the red cell membranes controls whole blood
viscosity.
During aging, there are significant alterations in blood coagulation
and hemostasis. One of the best known modifications of blood co
agulation is a typical thrombophilic tendency with aging.
For this symposium, we brought together 25 scientists from eight
countries. As gerontology is an interdisciplinary subject, workers of
different disciplines were invited. I hope that the papers and discussions
from this meeting will expand our knowledge of red cells, immunology,
receptors, blood coagulation, and rheology. I except that this book will
provide a firm foundation for future work in the area.
Finally, I wish to thank the pharmaceutical industry, especially
Dr. Bauer and Mr. Hansen, for the financial assistance provided for the
meeting.
Erlangen-Niimberg DIETER PLATT
Contents
Hemopoiesis as a Model for the "Biological Clock".
D. DANON, A. KNYSZYNSKI, and L. HAYFLICK
With 6 Figures . . . . . . . . . . . . . . . . . 1
Red Cell Membrane Skeleton: Assembly and Function.
J. PALEK. . . . . . . . . . . . . . . . . . . . . . . . . 13
Red Cell Membrane Lipids and Aging.
H. HECKERS and D. PLATT
With 4 Figures and 6 Tables . . . . . . . . 16
Red Cell Membrane Proteins, Glycoproteins, and Aging.
D. PLATT and W. RIECK
With 9 Figures and 1 Table . . . . . . . . . . . . . . . . 29
The Effect of Host and Cell Age on the Rat Erythrocyte:
Biochemical Aspects. D. GERSHON, G. A. GLASS, and H. GERSHON
With 5 Tables . . . . . . . . . . . . . . . . . . . 42
The Effect of Host and Cell Age on the Rat and Human
Erythrocyte: Cellular and Immunologic Aspects.
H. GERSHON, G. A. GLASS, and D. GERSHON
With 3 Figures and 5 Tables . . . . . . . . . . . . . . . . 51
Removal of Old and Abnormal Red Blood Cells from Circulation:
Mechanical and Immunologic Mechanisms. D. DRENCKHAHN
With 8 Figures and 1 Table . . . . . . . . . . . . . 62
Mechanism for Elimination of Senescent Red Blood Cells
from Circulation. N. KHANSARI
With 1 Figure and 6 Tables . . . . . . . . . . .. ... 77
The Influence of Pi race tam on the Age-Dependent Rate
of Phagocytosis (in the Isolated Perfused Rat Liver).
T. VOMEL and D. PLATT
With 4 Figures and 3 Tables . . . . . . . . . . . . . . . . 90
XII Contents
Changes in Human Red Cell Mechanics During In Vivo Aging:
Possible Influence on Removal of Senescent Cells.
G. B. NASH, O. LINDERKAMP, C. PFAFFEROTH, and H. J. MmsELMAN
With 4 Figures and 3 Tables . . . . . . . . . . . . . . . . 99
Age-Related Physiologic and Molecular Changes in the Thymus.
T. MAKINODAN, N. KINOHARA, and M.-P. CHANG
With 5 Figures and 1 Table . . . . . . . . . . . . . . . . 113
The Cellular Basis and Clinical Significance of Immune Senescence.
M. E. WEKSLER . . . . . . . . . . . . . . . . . . .. 122
Regulation of the Immune and Nonimmune Aging Process
by the Major Histocompatibility Complex. R. L. WALFORD
With 3 Tables . . . . . . . . . . . . . . . . . .. 127
Age-Related Morphologic Changes in Lymphocytes.
E. BEREGI
With 9 Figures . . . . . . . . . . . . . . . . . 139
Receptor and Postreceptor Changes During Lymphocyte Aging.
G. S. ROTH ........................ 150
Mechanism of Red Cell Aggregation.
D. E. BROOKS .......... . . 158
Fluidity of Blood of Elderly Patients and Rheologic Therapies.
H. KIESEWETTER, F. JUNG, N. KORBER, H. P. NUTTGENS, S. WOLF,
R. SCHNEIDER, and E. B. RINGELSTEIN
With 9 Tables . . . . . . . . . . . . . . . . . . . .. 163
Hemostasis, Microcirculation, and Thrombophilia in the Aged.
P. DE NICOLA and G. CASALE
With 7 Figures . . . . . . . . . . . . . . . . . . . .. 177
Influence of Age, Sex, and Blood Group on Blood Coagulation.
TH. B. WAHLBERG
With 3 Figures . . . . . . . . . . . . . . . . . . .. 194
Fibrinogen in the Aged - Functional and Structural Aspects.
K. HAGER, I. HERMANN, and D. PLATT
With 4 Figures and 4 Tables 201
SUbject Index. . . . . . . 215
Hemopoiesis as a Model for the "Biological Clock"
D. DANON, A. KNYSZYNSKI, and L. HAYFLICK
Introduction
Cultured normal human cells have a finite capacity for replication in vitro [1]. It
has been shown that embryo-derived fibroblasts which have the greatest capacity
±
for replication in vitro, undergo 50 10 population doublings before division
ceases in about 10-12 months. The events are divided into three phases. Phase I
represents the primary culture, phase II the active replication period, and phase
III the period when cell replication slows, ceases, and ultimately ends with com
plete cell degeneration and death. It was suggested that the phase III phenomenon
might represent aging at the cellular level [1, 2]. Research conducted in the ensu
ing years has confirmed the finitude of normal cell replication in vitro and has
substantially supported the suggestion that the phenomenon represents aging at
the cellular level (for reviews see refs. 3-8). The finitude of normal cell replication
in vitro implies the existence of a "biological clock."
The erythroid nucleus is expelled during the process of differentiation of the
mammalian erythrocyte [9, 10]. There are four discrete divisions in the develop
ment of mature erythrocytes. The stem cells divide into proerythroblasts in which
a small amount of hemoglobin can be detected in the cell cytoplasm. The proery
throblasts differentiate into basophilic erythrocytes in which the chromatin is
somewhat coarse and nucleoli are no longer discernible. As hemoglobin produc
tion increases the cells become polychromatic erythrocytes with densely staining
chromatin bodies in the nucleus. In the final stage of differentiation, the cells, cal
led normoblasts, have almost the same amount of hemoglobin as the mature
erythrocytes and the chromatin is denser and more compact than in the polychro
matophilic cells. Eventually the nuclei become pyknotic, and are extruded from
the cells along with a narrow rim of cytoplasm and apparently intact outer cell
membrane. The cytoplasmic components and the reticulocytes mature into eryth
rocytes and the expelled nuclei are soon phagocytized by macrophages.
The process of nuclear expulsion has been studied in several laboratories, in
cluding our own [11]. This process represents an ultimate differentiation of a cell
in which the genetic material is eliminated after four divisions. There is, in this
phenomenon, a definite and a very precise limit to the number of population dou
blings of the erythroid cells in vivo. Similar to phase III in the tissue cultured nor
mal fibroblasts, the expulsion of the erythroid nucleus ends the cell divisions. This
phenomenon may therefore be analogous to the in vivo expression of a "biolog
ical clock." An important question now arises: Is the nucleus expelled because it
died and is eliminated from the cell as a deteriorated organelle, or is the mecha-
Blood Cells, Rheology, and Aging
Ed. by D. Platt
© Springer-Verlag Berlin Heidelberg 1988