Biotechnology : Academic Cell Update Edition

767 Pages·2012·88.12 MB·English

by  David P Clark

Biotechnology
Academic Cell Update
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Biotechnology
Academic Cell Update
Authors
David P. Clark
Department of Microbiology
Southern Illinois University
Carbondale, Illinois
Nanette J. Pazdernik
School of Medicine
Washington University
St. Louis, Missouri

AMSTERDAM • BOSTON • HEIDELBERG • LONDON
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Academic Press is an imprint of Elsevier
Academic Press is an imprint of Elsevier
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This book and the individual contributions contained in it are protected under copyright by the
Publisher (other than as may be noted herein).
Notices
Knowledge and best practice in this field are constantly changing. As new research and experience
broaden our understanding, changes in research methods, professional practices, or medical treatment
may become necessary.
Practitioners and researchers must always rely on their own experience and knowledge in evaluating
and using any information, methods, compounds, or experiments described herein. In using such
information or methods they should be mindful of their own safety and the safety of others, including
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ISBN: 978-0-12-385063-8
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This book is dedicated to Donna. —DPC
This book is dedicated to my children and husband. Their patience and understanding have given me the
time and inspiration to research and write this text. —NJP
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3
aCChaadpetmeirC Cell
how we Got here
In speaking with professors across the biological sciences and going to conferences, we,
the editors at Academic Press and Cell Press, saw how often journal content was being
incorporated in the classroom. We understood the benefits students were receiving by being
exposed to journal articles early: to add perspective, improve analytical skills, and bring the
most current content into the classroom. We also learned how much additional preparation
time was required on the part of instructors finding the articles, then obtaining the images for
presentations and providing additional assessment.
So we collaborated to offer instructors and students a solution, and Academic Cell was born.
We offer the benefits of a traditional textbook (to serve as a reference to students and a
framework to instructors), but we also offer much more. With the purchase of every copy
of an Academic Cell book, students can access an online study guide containing relevant,
recent Cell Press articles and providing bridge material to help ease them into the articles. In
addition, the images from the articles are available in PowerPoint and we have optional test
bank questions.
We plan to expand this initiative, as future editions will be further integrated with unique
pedagogical features incorporating current research from the pages of Cell Press journals into
the textbook itself.
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B978-0-444-51911-5.00030-X
1
CONTENTS
PREFACE ........................................................................................................................................................................xi
ACKNOWLEDGMENTS .............................................................................................................................................xiii
INTRODUCTION ..........................................................................................................................................................xv
CHAPTER 1 Basics of biotechnology .......................................................................................................1
CHAPTER 2 DNA, RNA, and protein ......................................................................................................33
CHAPTER 3 Recombinant DNA technology .......................................................................................59
CHAPTER 4 DNA synthesis in vivo and in vitro .................................................................................93
CHAPTER 5 RNA-based technologies ................................................................................................125
CHAPTER 6 Immune technology ..........................................................................................................173
CHAPTER 7 Nanobiotechnology .........................................................................................................205
CHAPTER 8 Genomics and gene expression .....................................................................................231
CHAPTER 9 Proteomics .........................................................................................................................269
CHAPTER 10 Recombinant proteins .....................................................................................................305
CHAPTER 11 Protein engineering .........................................................................................................329
CHAPTER 12 Environmental biotechnology .......................................................................................349
CHAPTER 13 Pathway engineering .......................................................................................................371
CHAPTER 14 Transgenic plants and plant biotechnology ...............................................................397
ix
CHAPTER 15 Transgenic animals ..........................................................................................................425
CHAPTER 16 Inherited defects ..............................................................................................................457
CHAPTER 17 Gene therapy .....................................................................................................................477
CHAPTER 18 Molecular biology of cancer ..........................................................................................499
CHAPTER 19 Noninfectious diseases ...................................................................................................523
CHAPTER 20 Aging and apoptosis ........................................................................................................549
CHAPTER 21 Bacterial infections ..........................................................................................................579
CHAPTER 22 Viral and prion infections ................................................................................................597
CHAPTER 23 Biowarfare and bioterrorism .........................................................................................619
CHAPTER 24 Forensic molecular biology ............................................................................................645
CHAPTER 25 Bioethics in biotec hnology .............................................................................................665
BIOTECHNOLOGY Multiple-choice answer key...............................................................................................695
GLOSSARY ................................................................................................................................................................697
INDEX ..........................................................................................................................................................................739