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Biotechnology: Academic Cell Update Edition PDF

767 Pages·2012·36.95 MB·English
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Biotechnology Academic Cell Update This page intentionally left blank Biotechnology Academic Cell Update Authors David P. Clark Department of Microbiology Southern Illinois University Carbondale, Illinois Nanette J. Pazdernik School of Medicine Washington University St. Louis, Missouri AMSTERDAM • BOSTON • HEIDELBERG • LONDON NEW YORK • OXFORD • PARIS • SAN DIEGO SAN FRANCISCO • SINGAPORE • SYDNEY • TOKYO Academic Press is an imprint of Elsevier Academic Press is an imprint of Elsevier 30 Corporate Drive, Suite 400, Burlington, MA 01803, USA 525 B Street, Suite 1800, San Diego, California 92101-4495, USA 84 Theobald’s Road, London WC1X 8RR, UK © 2012 Elsevier Inc. All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Details on how to seek permission, further information about the Publisher’s permissions policies and our arrangements with organizations such as the Copyright Clearance Center and the Copyright Licensing Agency, can be found at our website: www.elsevier.com/permissions . This book and the individual contributions contained in it are protected under copyright by the Publisher (other than as may be noted herein). Notices Knowledge and best practice in this field are constantly changing. As new research and experience broaden our understanding, changes in research methods, professional practices, or medical treatment may become necessary. Practitioners and researchers must always rely on their own experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein. In using such information or methods they should be mindful of their own safety and the safety of others, including parties for whom they have a professional responsibility. To the fullest extent of the law, neither the Publisher nor the authors, contributors, or editors, assume any liability for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the material herein. Library of Congress Cataloging-in-Publication Data Application submitted British Library Cataloguing-in-Publication Data A catalogue record for this book is available from the British Library. ISBN: 978-0-12-385063-8 For information on all Academic Press publications visit our Web site at w ww.elsevierdirect.com Printed in China 10 11 12 13 9 8 7 6 5 4 3 2 1 This book is dedicated to Donna. —DPC This book is dedicated to my children and husband. Their patience and understanding have given me the time and inspiration to research and write this text. —NJP This page intentionally left blank 3 aCChaadpetmeirC Cell how we Got here In speaking with professors across the biological sciences and going to conferences, we, the editors at Academic Press and Cell Press, saw how often journal content was being incorporated in the classroom. We understood the benefits students were receiving by being exposed to journal articles early: to add perspective, improve analytical skills, and bring the most current content into the classroom. We also learned how much additional preparation time was required on the part of instructors finding the articles, then obtaining the images for presentations and providing additional assessment. So we collaborated to offer instructors and students a solution, and Academic Cell was born. We offer the benefits of a traditional textbook (to serve as a reference to students and a framework to instructors), but we also offer much more. With the purchase of every copy of an Academic Cell book, students can access an online study guide containing relevant, recent Cell Press articles and providing bridge material to help ease them into the articles. In addition, the images from the articles are available in PowerPoint and we have optional test bank questions. We plan to expand this initiative, as future editions will be further integrated with unique pedagogical features incorporating current research from the pages of Cell Press journals into the textbook itself. vvvvviiiiiiiiii This page intentionally left blank B978-0-444-51911-5.00030-X 1 CONTENTS PREFACE ........................................................................................................................................................................xi ACKNOWLEDGMENTS .............................................................................................................................................xiii INTRODUCTION ..........................................................................................................................................................xv CHAPTER 1 Basics of biotechnology .......................................................................................................1 CHAPTER 2 DNA, RNA, and protein ......................................................................................................33 CHAPTER 3 Recombinant DNA technology .......................................................................................59 CHAPTER 4 DNA synthesis in vivo and in vitro .................................................................................93 CHAPTER 5 RNA-based technologies ................................................................................................125 CHAPTER 6 Immune technology ..........................................................................................................173 CHAPTER 7 Nanobiotechnology .........................................................................................................205 CHAPTER 8 Genomics and gene expression .....................................................................................231 CHAPTER 9 Proteomics .........................................................................................................................269 CHAPTER 10 Recombinant proteins .....................................................................................................305 CHAPTER 11 Protein engineering .........................................................................................................329 CHAPTER 12 Environmental biotechnology .......................................................................................349 CHAPTER 13 Pathway engineering .......................................................................................................371 CHAPTER 14 Transgenic plants and plant biotechnology ...............................................................397 ix CHAPTER 15 Transgenic animals ..........................................................................................................425 CHAPTER 16 Inherited defects ..............................................................................................................457 CHAPTER 17 Gene therapy .....................................................................................................................477 CHAPTER 18 Molecular biology of cancer ..........................................................................................499 CHAPTER 19 Noninfectious diseases ...................................................................................................523 CHAPTER 20 Aging and apoptosis ........................................................................................................549 CHAPTER 21 Bacterial infections ..........................................................................................................579 CHAPTER 22 Viral and prion infections ................................................................................................597 CHAPTER 23 Biowarfare and bioterrorism .........................................................................................619 CHAPTER 24 Forensic molecular biology ............................................................................................645 CHAPTER 25 Bioethics in biotechnology .............................................................................................665 BIOTECHNOLOGY Multiple-choice answer key...............................................................................................695 GLOSSARY ................................................................................................................................................................697 INDEX ..........................................................................................................................................................................739

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Now available with the most current and relevant journal articles from Cell Press, Biotechnology Academic Cell Update Edition approaches modern biotechnology from a molecular basis, which grew out of the increasing biochemical understanding of physiology. Using straightforward, less-technical jargon
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