Biosensors B Understanding the importance and application of biosensors is Biosensors Understanding the importance and application of biosensors is B Biosensors compUlnicdaetersdt abnyd thineg d ivtheer sei mrapnogret aonf mcee tahnodd s aapnpdl aicpaptiloicna tioofn abniods seonmsoer s is iB complicated by the diverse range of methods and application and some o i existing text is somewhat technical in nature with little consideration to complicated by the diverse range of methods and application and some io existing text is somewhat technical in nature with little consideration to so and the novice. In Biosensors and Cancer we aim to disseminate the existing text is somewhat technical in nature with little consideration to e s and the novice. In Biosensors and Cancer we aim to disseminate the s and information on biosensors in a readable way by having unique sections the novice. In Biosensors and Cancer we aim to disseminate the e n information on biosensors in a readable way by having unique sections e for thinef noormviaceti aonnd o nex bpioerste anlsiokres. i Tnh ai sre eandaabbleles wthaey r beayd hearv tion tgr aunnsifqeur eth seeicrt ions n for the novice and expert alike. This enables the reader to transfer their sn knowfoler dthgee nboavsiec efr aonmd oenxpe edrits acliipklein. e T thoi sa ennoathbelers o trh efr roemad oenr eto a tcraadnesmfeirc t heir o s Cancer knowledge base from one discipline to another or from one academic s Cancer levelk ntoo walneodtghee rb. aEsea cfrho mch oanpete dr isicni pBliinoes eton saonrost haenrd o rC fraonmce or nhea asc aadne mic o Cancer level to another. Each chapter in Biosensors and Cancer has an ro abstrlaecvte, lk etoy faancotst,h aepr.p lEicaacthio nchs atpot oerth einr aBreioasse onfs hoeras ltahn adn dC danisceears eh, aas an s r abstract, key facts, applications to other areas of health and disease, a r “minaib-dstircaticotn, kareyy” f oacf tkse, ya pteprlmicsa tainodn ss utom omthaerry aproeianst so.f W hee ahlathv ea cnhda dpitseeras se, a s as “mini-dictionary” of key terms and summary points. We have chapters on b“iomseinnis-odrisc tbioanseadry o”n o fo kr euyt itleirzminsg a onpdt iscuaml immaargyi npgo,i nsutsr.f Wacee hpalvaesm choanp ters n a on biosensors based on or utilizing optical imaging, surface plasmon a resonoann bcieo, smenicsroorcsa bnatisleedv eorns, oerle ucttriloizchinegm oispttriyc,a al pimtamageinrsg, ,f lsuuorrfaesccee pnlcaes,m on n resonance, microcantilevers, electrochemistry, aptamers, fluorescence, dn electrreoscohneamnicset,r ym, icnraoncoanbtioilseevnesros,r es leacntrdo chneamnoiswtriyre, as.p tTamheerres , falrueo raelsscoe nce, d electrochemistry, nanobiosensors and nanowires. There are also Cd chapters on oxidative damage to DNA, miRNA, leukemia, breast electrochemistry, nanobiosensors and nanowires. There are also chapters on oxidative damage to DNA, miRNA, leukemia, breast C cancer, BCR-ABL activity, single living cells and thyroid cancer. Drug aC chapters on oxidative damage to DNA, miRNA, leukemia, breast cancer, BCR-ABL activity, single living cells and thyroid cancer. Drug a discovery, cancer diagnosis, anticancer drugs, and cancer detection n cancer, BCR-ABL activity, single living cells and thyroid cancer. Drug a discovery, cancer diagnosis, anticancer drugs, and cancer detection n idendtiifsycionvge rmy,a rckaenrc e mr odleiacgunleoss i fso, ra pntriocsatnactee r c adnrucegrs , a aren d a lcsoan ccoevr edreedte. c tion cn identifying marker molecules for prostate cancer are also covered. e c identifying marker molecules for prostate cancer are also covered. c Contributors to Biosensors and Cancer are all either international or e r Contributors to Biosensors and Cancer are all either international or e natioCnoanl treixbpuetrotrss, tloe aBdioinsge nasourths oarnitdie Cs aonrc ear raer ec aarllr yeiinthge ro iuntt ergnroatuionnda l or r national experts, leading authorities or are carrying out ground r breaking and innovative work on their subject. The book is essential national experts, leading authorities or are carrying out ground breaking and innovative work on their subject. The book is essential reading for oncologists, cancer workers and scientists, medical doctors, breaking and innovative work on their subject. The book is essential reading for oncologists, cancer workers and scientists, medical doctors, health care professionals, pathologists, biologists, biochemists, reading for oncologists, cancer workers and scientists, medical doctors, health care professionals, pathologists, biologists, biochemists, chemists and physicists, general practitioners as well as those health care professionals, pathologists, biologists, biochemists, chemists and physicists, general practitioners as well as those interested in disease and sciences in general. chemists and physicists, general practitioners as well as those VV inteirnetsetreeds t eind d inis edaissee a asne d a n sdci e snccieens c iens g ine n geernale.r al. inood ictor RVinoEdiVictoVinooVictorE B. otordE Rd K16060 . PatelPreedyd B. Paters R. Preed B. Patelditors . Preedyitors VViicntooEro ddVRi iBt.Eco P.trd osPrEi rteao deRtrdiest.lyo Prsr eedy ly Victor R. Preedy Vinood B. Patel Vinood B. Patel 6000 Broken Sound Parkway, NW Suite 300, Boca Raton, FL 33487 711 Third Avenue New York, NY 10017 an informa business 2 Park Square, Milton Park A ScieNce PUBLiSheRS BOOK www.taylorandfrancisgroup.com Abingdon, Oxon OX14 4RN, UK Biosensors and Cancer TThhiiss ppaaggee iinntteennttiioonnaallllyy lleefftt bbllaannkk Biosensors and Cancer Editors Victor R. Preedy PhD DSc Professor of Nutritional Biochemistry School of Medicine King’s College London and Professor of Clinical Biochemistry King’s College Hospital UK Vinood B. Patel Department of Biomedical Science School of Life Sciences University of Westminster London UK p, A SCIENCE PUBLISHERS BOOK GL--Prelims with new title page.indd ii 4/25/2012 9:52:40 AM CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2013 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Version Date: 20120710 International Standard Book Number-13: 978-1-4665-5808-3 (eBook - PDF) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, microfilming, and recording, or in any information stor- age or retrieval system, without written permission from the publishers. For permission to photocopy or use material electronically from this work, please access www.copy- right.com (http://www.copyright.com/) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that pro- vides licenses and registration for a variety of users. For organizations that have been granted a pho- tocopy license by the CCC, a separate system of payment has been arranged. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation without intent to infringe. Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com Preface Biosensors have a simplistic concept but a great deal of sophistication in design, manufacture and application. They essentially have biological components within them and are used to detect, monitor or quantify substances. They use a variety of physical platforms and technologies. The biological components may include enzymes, membranes and cells or any other naturally occurring biological product. Some have artifi cial biological components such as modifi ed molecules or polymers. Biosensors may be used to detect single or groups of molecules and have wide applicability to the life sciences. Each chapter in Biosensors and Cancer has an abstract, key facts, applications to other areas of health and disease and a “mini- dictionary” of key terms and phrases within each chapter. Finally, each chapter has a series of summary points. In this book focussing on cancer we have chapters on biosensors based on or utilizing optical imaging, surface plasmon resonance, microcantilevers, electrochemistry, aptamers, fl uorescence, electrochemistry, nanobiosensors and nanowires. There are also chapters on oxidative damage to DNA, miRNA, leukemia, breast cancer, BCR-ABL activity, single living cells and thyroid cancer. Drug discovery, cancer diagnosis, anticancer drugs, and cancer detection identifying marker molecules for prostate cancer are also covered. Contributors to Biosensors and Cancer are all either international or national experts, leading authorities or are carrying out ground breaking and innovative work on their subject. The book is essential reading for oncologists, cancer workers and scientists, medical doctors, health care professionals, pathologists, biologists, biochemists, chemists and physicists, general practitioners as well as those interested in disease and sciences in general. The Editors TThhiiss ppaaggee iinntteennttiioonnaallllyy lleefftt bbllaannkk Contents Preface v List of Contributors xi Section 1: General 1. Functional Optical Imaging-based Biosensors 3 Pablo Iglesias and Jose A. Costoya 2. Use of a Surface Plasmon Resonance (SPR) Biosensor to 20 Characterize Zwitterionic Coatings on SiO for Cancer 2 Biomarker Detection Norman D. Brault, Shaoyi Jiang and Qiuming Yu 3. Microcantilever-based Biosensor Array for Tumor 43 Angiogenic Marker Detection Riccardo Castagna and Carlo Ricciardi 4. Electrochemical DNA Biosensors at the Nanoscale 62 Rosa Letizia Zaffi no, Wilmer Alfonso Pardo, Mònica Mir and Josep Samitier 5. Aptamer-based Biosensors for Cancer Studies 85 Ilaria Palchetti and Marco Mascini 6. Fluorescent Biosensors for Cancer Cell Imaging and 101 Diagnostics May C. Morris 7. Electrical and Electrochemical Immunosensor for 125 Cancer Study Seung Yong Lee and Seung Yong Hwang 8. Multifunctional Nanobiosensors for Cancer 146 Dai-Wen Pang and Er-Qun Song viii Biosensors and Cancer 9. Silicon Nanowire Biosensor for Cancer Markers 164 Yang-Kyu Choi and Chang-Hoon Kim Section 2: Blood, Molecules and Cells 10. DNA-electrochemical Biosensors and Oxidative Damage 187 to DNA: Application to Cancer Victor Constantin Diculescu and Ana Maria Oliveira Brett 11. Asparaginase-based Asparagine Biosensors and Their 211 Application to Leukemia Neelam Verma and Kuldeep Kumar 12. Breast Cancer Detection Using Surface Plasmon 229 Resonance-Based Biosensors Chii-Wann Lin and Chia-Chen Chang 13. Detection of miRNA with Silicon Nanowire Biosensors 248 Guo-Jun Zhang 14. Biosensors for BCR-ABL Activity and Their Application to 268 Cancer Yusuke Ohba, Stephanie Darmanin, Tatsuaki Mizutani, Masumi Tsuda and Takeshi Kondo 15. Optical Fiber Nanobiosensor for Single Living Cell 284 Detections of Cancers Xin Ting Zheng and Chang Ming Li 16. Microfl uidic Biosensors for Thyroglobulin Detection and 300 Application to Thyroid Cancer Seokheun Choi and Junseok Chae Section 3: Treatments and Organs Specifi c Applications 17. Optical Biosensors and Applications to Drug Discovery 321 and Development in Cancer Research Carlo Bertucci and Angela De Simone 18. Single-Chain Fragment Variable Recombinant Antibodies 337 and Their Applications in Biosensors for Cancer Diagnosis Xiangqun Zeng and Ray Mernaugh 19. DNA Biosensor for Rapid Detection of Anticancer Drugs 359 Sigen Wang and Ruili Wang Contents ix 20. Using UV Light to Engineer Biosensors for Cancer 378 Detection: The Case of Prostate Specifi c Antigen Maria Teresa Neves-Petersen, Antonietta Parracino and Steffen B. Petersen Index 395 About the Editors 401 Color Plate Section 403