Biometry Protocol Purpose Use appropriate mathematics to To measure and classify the plant life analyze data. present at a Land Cover Sample Site to help Develop descriptions and predictions determine the MUC classifcation. using evidence. Recognize and analyze alternative Overview explanations. Students walk the half-diagonals of their Communicate procedures, Land Cover Sample Site and take one or descriptions, and predictions. more biometry measurements. These may Level include canopy cover and ground cover, All identifying dominant and co-dominant vegetation species, and measuring tree Time circumference, GPS coordinates and height, and/or graminoid biomass. Variable, depending on type and number of measurements taken Student Outcomes Frequency Students will learn how to use biological sampling techniques to quantify and As necessary to determine MUC at most describe a Land Cover Sample Site. sites, or, frequently as an enrichment study Science Concepts Physical Science Materials and Tools Objects have observable properties 50 m tape measure that can be measured using tools. Compass Life Science Species ID keys and/or other local Earth has many different environments species guides that support many different kinds of MUC Field Guide or MUC System organisms. Table and MUC Glossary of Terms Organisms change the environment in GPS protocol which they live. GPS receiver All populations living together and the Stopwatch physical factors with which they GPS data sheet interact constitute an ecosystem. Permanent tree markers (optional) Geography Pen or pencil The physical characteristics of place Calculator (optional) The characteristics and spatial Appropriate Biometry Data Sheets distribution of ecosystems Tubular densiometer (See Scientifc Inquiry Abilities Investigation Instruments section) Identify biometry measurements Clinometer (See Investigation needed for MUC. Instruments section) Use vegetation feld guides to identify Flexible tape measure vegetation and species. Blindfold Interpret data to propose MUC Clipboard classifcation. Small bean bag Identify answerable questions. Grass clippers or strong scissors Design and conduct scientifc Small brown paper bags investigations. Drying oven GLOBE® 2014 Biometry Protocol- 1 Biosphere Welcome Introduction Protocols Learning Activities Appendix Balance or scale, accurate to 0.1 g Gather materials for clinometer and densiometer. Preparation Make copies of the appropriate Work Have students practice taking feld measurements, pacing and using a Sheets. compass. Familiarize students with the MUC System. Prerequisites size in both areas? Students make necessary feld instruments. Measurements of living things are important to scientists. They can show the amount of Site-Seeing Learning Activity Beginning or nutrients and gases living things store. They Intermediate (Suggested) also show the amount of carbon and usable water stored in trees and plants. Biometry Protocol – Choosing the right MUC class can be hard. Introduction How do you know that you have a “deciduous forest” and not an “evergreen forest”? How Biometry is the measuring of living things. do you know you are in a “shrubland” and Why do scientists need measurements of not “woodland”? How do you know a site is living things? What do they tell us about our “tall graminoid” and not “short graminoid”? environment? The biometry measurements Biometry measurements answer these include tree height and circumference, canopy questions. cover, ground cover, and graminoid biomass. Graminoids are grass and grass-like plants. Biometry measurements help you choose the These all measure the size or amount of trees right MUC class. What kinds of measurements and plants. will you need to decide between an evergreen and deciduous forest? What measurements What do trees and plants store? What are they will you need to decide if something is a shrub made of? Can different types of land cover or a tree? What do you need to know to decide have different size trees, shrubs or grasses? between a tall and short graminoid site? Can they have different amounts of trees, Biometry measurements make your data shrubs or grasses? Think about a desert. What more reliable. When scientists use your is the most common tree or shrub there? Is Land Cover Sample Site data, biometry that a sign of what kind of area it is? Compare measurements assure them that the data that to the most common tree in a forest. are of high quality. There are two tests of Can the same types of land cover have good measurement technique. Biometry different size trees, shrubs or grasses? Can measurements will help assess how close there be different amounts of trees, shrubs or to the bull’s-eye (the right answer) your data grasses? Think about two wetlands. Are the are. This is called accuracy. Your data are trees, shrubs or grasses the same type and precise when you repeat measurements and High Accuracy and High Accuracy and Low Accuracy and Low Precision High Precision High Precision GLOBE® 2014 Biometry Protocol - 2 Biosphere get the same results throughout a site. The vegetation biomass estimates through the goal of GLOBE students should be to have measurement of vegetation canopy height. their measurements look like the bull’s eye in In closed forest sites, where the trees cover the center (see below), highly accurate and the shrubs and ground, the trees will refect precise! Biometry measurements can help the highest percentage of light. See Figure you do that. BIO-BI-2. In woodland sites, where there is space between trees, there will be a greater contribution of refectance values from the Teacher Support shrubs and ground below the tree canopy, but again, the trees will refect the greatest The Measurement percentage of light. See Figure BIO-BI-3. In The Biometry Protocol is divided into shrubland sites, where shrubs dominate, the four different measurements: 1) canopy refectance values will mostly represent the and ground cover; 2) tree, shrub and/or shrubs and not the scattered trees or ground graminoid height; 3) tree circumference; and cover that may also be present at the site. 4) graminoid biomass. You may choose to See Figure BIO-BI-4. Keeping this in mind take biometry measurements only once in a should help you determine what biometry site during peak growth, or you can return to measurements to take. For instance, in a the same site year after year and repeat the closed forest site with tall trees covering the biometry measurements to track changes entire site, shrubs scattered throughout the in the site biomass over time. You may also site below the trees and some tall grasses on take biometry measurements twice a year in the forest foor, the biometry measurements a single site year after year, once during peak that would be most important would be tree foliage or growth and once during a time of canopy, ground cover, and tree height. You low growth (i.e. winter or drought). You should may choose to measure shrub height or always use the following two guidelines to graminoid biomass but since the tree canopy determine what measurements you should would dominate the refectance values, the take: shrub and graminoid data would be less important. Another example would be in First, take ANY measurements necessary to herbaceous vegetation sites. See Figure BIO- determine the correct MUC class. Whenever BI-5. If a site was primarily graminoid with a decision must be made between MUC two trees and several shrubs, the most useful classes, take the appropriate biometry biometry measurement would be graminoid measurement (i.e. canopy and ground biomass. You can also measure the height cover or height) to make that decision. If of the shrubs and trees, but since they are the decision can be made without biometry not the dominant land cover, the graminoids measurements, it is not necessary to take would refect the highest percentage of light any, but you may choose to do so to ensure in that area. However, it would still be useful accuracy. to note that the site contained the trees and Second, scientists will be using an aerial shrubs. Any type of information of this sort view when using the MUC and biometry is important metadata since sites that are data and you should too. Therefore, purely herbaceous vegetation may refect measurements of the dominant (covering light slightly differently than sites that have the greatest area) land cover of the highest a few trees or shrubs. (Note: If you use any canopy are the most important. Canopy canopy and ground cover measurements cover refers to layers of vegetation. There to determine the MUC class, report that can be several layers of each type (tree measurement also.) and/or shrub). Multiple layers are present when canopy heights are at different levels. Student Preparation When these layers are not present, the Students should be able to defne and identify ground cover forms the dominant vegetation a homogeneous land cover site. type. See Figure BIO-BI-1. the Landsat Students should understand and know how to satellite records the amount and wavelength classify a site using the MUC System. of light refected be ALL vegetation that it can “see.” ICESat-2 provides large-scale GLOBE® 2014 Biometry Protocol - 3 Biosphere Welcome Introduction Protocols Learning Activities Appendix Figure BIO-BI-1: Multiple Layers of Vegetation: Tree Canopy, Shrub Canopy and Ground Cover Side View LandSat Satellite ICESat-2 Satellite 5 m Ground Cover The tree canopy will meet the height requirement of greater than 5 m while shrubs are between 0.5 m and 5 m tall. Top View Figure BIO-BI-2: Closed Forest Site Side View The most useful biometry measurements to take here would be tree canopy, tree height and ground cover. Top View ® GLOBE 2014 Biometry Protocol - 4 Biosphere Figure BIO-BI-3: Woodland Site Side View The most useful biometry measurements to take here would be tree canopy, tree height and ground cover. Top View Figure BIO-BI-4: Shrubland Site Side View 5 m The most useful biometry measurements to take here would be shrub canopy, shrub height and ground cover. Top View Figure BIO-BI-5: Herbaceous Site Side View The most useful biometry measurements would be ground cover and graminoid biomass. Top View ® GLOBE 2014 Biometry Protocol - 5 Biosphere Students should make and know how to use the seen at the densiometer crosshairs. densiometer and clinometer. Determine the percentage of trees in the canopy (# of +’s / total number of Students should know how to use a compass. observations from the Tree Canopy Students should practice pacing techniques. and Ground Cover Data Sheet). If They should know their pace and how many the trees are 40% or more of the paces are in 21.2 meters. canopy, this is a forest or woodland and you should use the Tree Canopy Helpful Hints and Ground Cover Data Sheet for • Practice these measurements in a the ground cover data collection on location close to school to get some the half-diagonals and report all of experience before using them in a Land these measurements. If trees are Cover Sample Site. less than 40% of the canopy, walk the half-diagonals again using the Shrub • You or your students may want to Canopy and Ground Cover Data investigate a potential site with a brief Sheet. Record a (+) where a shrub is visit to make sure that it is large enough seen at the densiometer crosshairs. and homogeneous throughout before a Determine the percentage of shrubs in longer data collection visit. the canopy (# of +’s / total number of • When distinguishing between trees observations from the Shrub Canopy and shrubs, use the defnition of a tree and Ground Cover Data Sheet). If given in the MUC Field Guide and MUC the shrubs are greater than 40% of Glossary of Terms: a tree is at least 5 the canopy, this is a shrubland and meters tall. You may want to practice you should use the Shrub Canopy estimating this height with the clinometer and Ground Cover Data Sheet for the near your school before entering the ground cover data collection on the feld. half-diagonals and report all of these • If the shrub canopy is below the measurements. observer, treat it as ground cover. Dwarf- • If both trees and shrubs are less shrubs are always considered ground than 40%, choose the Data Sheet cover. corresponding with the highest • There are two Canopy and Ground percentage of canopy cover to take Cover Data Sheets: one to use when the ground cover measurements. the dominant canopy cover is trees and Example: In a site with 15% tree one to use when the dominant canopy canopy and 35% shrub canopy, use cover is shrubs. Your students need to the Shrub Canopy and Ground Cover decide which one to use. In a forest or Data Sheet to take your ground cover woodland, canopy cover refers to the measurements and report the tree tree canopy. In a shrubland, canopy and shrub canopy cover as metadata. refers to the shrub canopy. Always keep Since the tree and shrub canopy make in mind that these measurements are up less than 40% of the canopy, the helpful to scientists studying land cover MUC class will not be a Closed Forest, from satellite imagery. Therefore, the Woodland or Shrubland. In this case, highest canopy is what you should try to use the ground cover measurements measure. to determine the correct MUC class. • If you have diffculty determining if a site • It is more effcient to have your is a forest, woodland or shrubland, you students work in pairs or trios for this may need to walk the half-diagonals protocol. twice. Keep in mind the “view from • For more accurate readings, other above” and look to the highest canopy pairs of students should repeat the with your densiometer for the correct measurements. If different teams of measurement. The frst time, use the students repeat observations, report Tree Canopy and Ground Cover Data the average of these values if they Sheet recording a (+) where a tree is generally agree. ® GLOBE 2014 Biometry Protocol - 6 Biosphere • Before going into the feld, teach measurements with a class, break your students how to use your local the class into groups and have each vegetation feld guides. group pace a different half-diagonal. • It is recommended that you consult Each group will need its own copy of local experts (Forest Service, County the Field Guide, a Data Sheet, and Extension Agent, etc.) to assist with a densiometer. Ideally, one person species identifcation. should serve as a ‘pacer’ and one • If your site experiences seasonal should be the ‘recorder.’ The ‘pacer’ variation and you choose to track walks the distance and makes the changes in biomass over time, take measurements. The ‘recorder’ records biometry measurements once during the readings onto the Data Sheet peak growing season and once during and makes sure the ‘pacer’ is walking the least active season. straight in the assigned direction. The ‘pacer’ should know how many of his/ vvWhen measuring the tree height, her paces are in the 21.2 m length of GPS measurements, using the GPS the half-diagonal. Have each student Protocol, should be measured at the write this number on his/her copy base of each of the trees. Remember, of the Field Guide. This is the total the trees being measured must be at number of measurements/paces to least 3 meter away from each other. take in walking a half-diagonal from • If it takes smaller students more than the center to the corner of the central forty paces to complete a diagonal, 30 m x 30 m area. they may take measurements at every other pace. Questions for Further Investigation • For younger students, if the angle What are the dominant and co-dominant on the clinometer is 45 degrees, the species in your Land Cover Sample Site? distance from the tree will equal the Do these species always occur in sites that height of the tree above the student’s have the same MUC class? eye level. See the Alternative Technique to Measure Tree Height on Are the dominant and co-dominant species Level Ground: Simplifed Clinometer common in your area? Are these species Technique Field Guide. native to your area? Are the trees mature or juvenile? • If you are going to revisit a forest or woodland site, mark and number/label Is there a relationship between the amount the trees you use. Always measure of ground cover and canopy cover? the same trees, and report their Are the percentages of the canopy and heights and circumferences in the ground cover consistent with your MUC same order. class? • Examples of forbs include clover, sunfowers, ferns, and milkweeds. Which is greater, the amount of brown or • Do not use a conventional oven to green ground cover? Do you think that these dry the graminoid vegetation. This is amounts will change during the year? dangerous because the oven may If your MUC 4 site has trees as the co- have to be left on continuously for dominant species: Is the herbaceous several days! vegetation around the trees the same as that • In warm, dry climates, graminoid in open areas? biomass samples can be dried in mesh bags outside. • Make sure to use several small brown drying bags for proper drying of graminoid samples. • If you are performing the Canopy Cover and Ground Cover ® GLOBE 2014 Biometry Protocol - 7 Biosphere Welcome Introduction Protocols Learning Activities Appendix Canopy Cover and Ground Cover Field Guide Task Take ground and canopy cover measurements while pacing half-diagonals to determine the MUC class of your Land Cover Sample Sites. What You Need q Tubular densiometer q Compass q Tree Canopy and Ground Cover q Species ID keys and/or other local species Data Sheet guides q Shrub Canopy and Ground Cover q Pen or pencil Data Sheet q Clipboard In the Field N Land Cover Sample Site with the four 21.2 m half- Center of Site diagonals (in the NE, SE, SW and NW directions) for sampling. 30 m • Locate the center of your homogeneous Land Cover Sample Site. This is your starting point. Take the measurements described in Steps 2 and 3 from the center of the Sample Site by walking the distance of a half-diagonal (21.2 m) in each of the following four directions: NE, SE, SW and NW (using a compass for bearing). Stop after each pace (2 steps) to complete Steps 2 and 3. • There are two possible data sheets to use for measuring Canopy and Ground Cover, the Tree Canopy and Ground Cover Data Sheet or the Shrub Canopy and Ground Cover Data Sheet. The following step will help you decide which one to use. However, if you are still confused about which sheet to use, you may want to choose a different site where the decision is clearer. GLOBE® 2014 Biometry Protocol - 8 Biosphere 21.2 m 30 m Canopy Cover and Ground Cover: Field Guide - Page 2 Steps: 1. Decide which Canopy and Ground Cover Data Sheet is appropriate, using the following procedure. a. If the site is clearly dominated (greater than 40%) by trees (greater than 5 m tall), use the Tree Canopy and Ground Cover Data Sheet. Go to Step 2. b. If the site is clearly dominated by shrubs (between 50 cm and 5 m) and there is less than 40% tree canopy, use the Shrub Canopy and Ground Cover Data Sheet. Go to Step 2. c. If you cannot decide the dominant canopy cover: • Walk each half-diagonal (21.2 m) from the center of your site using your densiometer and the Tree Canopy and Ground Cover Data Sheet. Follow Step 2, marking a (+) if you see a tree at the crosshairs of your desiometer and a (-) if there is not. Record the other canopy information for any trees you record a (+) for. • Calculate the percent tree canopy cover (# of +’s/total number of observations from the Tree Canopy and Ground Cover Data Sheet). If the percent tree cover is over 40%, use the Tree Canopy and Ground Cover Data Sheet and walk the half- diagonals again following Step 3 to collect the ground cover data. • If the trees are less than 40%, walk the half-diagonals again using the Shrub Canopy and Ground Cover Data Sheet. Follow Step 2, marking a (+) if you see a shrub at the crosshairs even if there is a tree above it and a (-) if there is not a shrub at the crosshairs. Record the other canopy information for any shrubs you record a (+) for. • Calculate the percent shrub canopy cover (# of +’s / total number of observations from the Shrub Canopy and Ground Cover Data Sheet). If the shrubs are dominant (40% or more), use the Shrub Canopy and Ground Cover Data Sheet to record the ground cover data by walking the half-diagonals again following Step 3. • If both trees and shrubs are less than 40%, choose the Data Sheet corresponding with the highest percentage of canopy cover and walk the half-diagonals again following Step 3. Report the percentage of tree and shrub canopy as metadata since they help scientists understand the site. Note: Since neither the tree nor shrub canopy are dominant, the MUC class of this site should not start with 0 (Closed Forest), 1 (Woodland), or 2 (Shrubland). 2. Look up through your densiometer at the canopy. Make sure the densiometer is vertical and the metal nut/washer is directly below the intersection of the crosshairs at the top of the tube. Look to the highest canopy. If you are using the Tree Canopy and Ground Cover Data Sheet, only record data for TREES and ignore shrubs. If you are using the Shrub Canopy and Ground Cover Data Sheet, only record data for SHRUBS and ignore trees. a. If you see vegetation, twigs or branches at the crosshairs: • Record a (+) on the Canopy and Ground Cover Data Sheet. Remember, if you are using the Tree Canopy and Ground Cover Sheet, only record a (+) if the vegetation you see is from a tree. If it is from a shrub, record a (-) instead and skip the next steps. The opposite will be true if you are using the Shrub Canopy and Ground Cover Sheet. • Identify the species name. If you do not know the genus and species but know the common name, record the common name. If you do not know the common name, collect a leaf or describe or sketch it for identifcation later in the classroom. • Record the canopy type as Evergreen (E) or Deciduous (D). b. If you do not see vegetation, twigs, or branches at the crosshairs: • Record a (-) on the Canopy and Ground Cover Data Sheet. 3. Stand with your feet shoulder-width apart, look down and observe any vegetation that is touching your foot or leg below the knee. Do not pick up your foot; only use ® GLOBE 2014 Biometry Protocol - 9 Biosphere Canopy Cover and Ground Cover: Field Guide – Page 3 vegetation touching you without moving. Also, do not measure ground cover by looking down through the densiometer! a. If the vegetation is green (alive), record a (G) on the Canopy and Ground Cover Data Sheet. b. If the vegetation is green, record if it is graminoid (GD), forb (FB), other green vegetation (OG), Shrub (SB) or Dwarf Shrub (DS). c. If the vegetation is brown but still attached, record a (B). d. If there is no vegetation, record a (-) on the Canopy and Ground Cover Data Sheet. 4. After you have completed your pacing and measurements, fll out the summary tables on the bottom of the Canopy and Ground Cover Data Sheet using the formulas below for reference in calculating percentages. Note: If other groups walked other half- diagonals, compare your “% Canopy Cover” and “% Ground Cover” with the other group’s diagonal data. Average the percentages from all the groups and use these averaged values to determine the dominant land cover for data entry. 5. If you have enough information to determine the MUC class of your site at this point, you are done. If tree, shrub or graminoid height cannot be estimated, follow the Field Guide for Graminoid, Tree and Shrub Height. Determining the Percentage of Tree or Shrub Canopy Cover (Column 1): Calculate the percent of tree or shrub canopy using the data collected. Use the following equation as a model. __#_ o_f_ +_’_s_ (_T_re_e_ _o_r _S_h_r_u_b_ C__a_n_o_p_y)_ % Tree or Shrub Canopy Cover = X 100 # of Total Observations Determining the Percentage of Evergreen or Deciduous Canopy Cover (Column 3): Calculate the percent of tree or shrub canopy that is evergreen or deciduous using the data collected. Use the following equation as a model. _____#_ _o_f _E_’s_ _(E_v_e_r_g_r_e_e_n_ O_b_s_e_r_v_a_ti_o_n_s_)__________ % Evergreen = X 100 # of E’s + # of D’s (Total Canopy Cover Observations) Determining the Percentage of Ground Cover (Column 4): Calculate the percent ground cover using the data collected. Use the following equation as a model. __#_ _o_f _G_’s_ _(G__re_e_n_)_ _+_ #_ _o_f _B_’s_ _(B_r_o_w_n_)_ % Ground Cover = X 100 # of Total Observations Determining the Composition of Herbaceous Coverage (Column 5): Calculate the percent of the ground that is graminoid, forb or other green vegetation using the data and the following equation as a model. _____#_ _o_f _G_D_’_s_ (_G_r_a_m_i_n_o_id_ _O_b_s_e_rv_a_t_io_n_s_)___________ % Graminoid = X 100 # of GD’s + # of FB’s + # of OG’s + # of SB’s + # of DS’s (Total Herbaceous Ground Observations) Determining Total Shrubs (Column 6): ____#_ o_f_ +_’s_ _(S__h_ru_b_s_ _P_re_s_e_n_t_)__ % Total Shrubs = X 100 # of Total Observations ® GLOBE 2014 Biometry Protocol - 10 Biosphere