Biomembranes Part W: Cellular and Subcellular Transport: Epithelial Cells

872 Pages·1990·25.067 MB·English

by  Sidney Fleischer, Becca Fleischer (Eds.)

Methods in Enzymology
Volume 192
Biomembranes
Part W
Cellular and Subcellular Transport:
Epithelial Cells
EDITED BY
Sidney Fleischer
Becca Fleischer
DEPARTMENT OF MOLECULAR BIOLffiY
VANDERBILT UNIVERSITY
NASHVILLE, TENNESSEE
Editorial Advisory Board
Yam0 Kagawa George Sachs
Ronald Kaback Antonio Scarpa
Martin Klingenberg Widmar Tanner
Robert L. Post Karl Ullrich
ACADEMIC PRESS, INC.
Harcourt Brace Jovanovich, Publishers
San Diego New York Boston
London Sydney Tokyo Toronto
Contributors to Volume 192
elcitrA srebmun are in parentheses following the names of contributors.
Affiliations fisted are current.
ZAHUR AHMED (21), Department of -loisyhP Medical School, Ann Arbor, Michigan
,ygo School of Medicine, State ytisrevinU 90184
of New York at ,olaffuB ,olaffuB New York MOIRA CIOFFI (40), Department of ,ygoloiB
41241 Temple ,ytisrevinU ,aihpledalihP Pennsyl-
G. ALTENnERa (43), Department of -loisyhP vania 22191
ogy and Biophysics, The University of REMITROM M. CIVAN (44), Departments of
Texas Medical Branch, ,notsevlaG Texas Physiology and Medicine, University of
05577 ainavlysnneP School of Medicine, Phila-
INDU S. AMBUDr.AR (3), Clinical -agitsevnI ,aihpled Pennsylvania 40191
tions and Patient Care Branch, National
MICHEL CLARET (31), INSERM U274, -nU
Institute of Dental Research, National In-
Otisrevi Paris-Sud, Orsay, 91405 Cedex,
stitutes of Health, Bethesda, Maryland
ecnarF
29802
LAURENT SETTEBMOC (31), INSERM U
B. E. ARGENT (17), Department of -oisyhP
274, OtisrevinU Paris-Sud, Orsay, 91405
lacigol Sciences, University of Newcastle
Cedex, France
upon Tyne, Newcastle upon Tyne NE2
4HH, England J. OLLEPOC (43), Department of ygoloisyhP
and Biophysics, The University of Texas
ECURB J. BAUM (3), Clinical snoitagitsevnI
Medical ,hcnarB ,notsevlaG Texas 05577
and Patient Care Branch, National Insti-
tute of Dental Research, National Insti- C. COTTON (43), Department of ,scirtaideP
tutes of Health, Bethesda, Maryland Case Western Reserve University, Cleve-
29802 ,dnal Ohio 60144
THOMAS BERGLINDH (6), Department of THGIWD N. DROFWARC (39), Department of
Biomedical Research, Pharmaia AB, ,ygoloiB Temple ,ytisrevinU ,aihpledalihP
S- 157 82 ,alasppU Sweden ainavlysnneP 22191
A. TOOLETREB (26), Department of -loisyhP
S. CURO (5), Istituto di aigoloisiF ,elareneG
ogy, Faculty of Medicine, University of
~tisrevinU di Bari, 62107 Bari, Italy
Montreal Montreal Quebec H3C 3T8,
adanaC ENIREHTAC TNOMEGRAD (31), Ecole Nor-
male ,erueirbpuS Paris ,60057 France
RICHARD C. BOUCHER (36), Department of
Medicine, The University of North -oraC DAVID C. DAWSON (47), Department of
lina at Chapel Hill, Chapel Hill, North ygoloisyhP and the ralulleC and raluceloM
aniloraC 27599 Biology Program, University of nagihciM
Medical School Ann Arbor, Michigan
J. L. BORER (32, 33, 34), Department of
90184
Medicine and Liver Center, Yale -revinU
sity School of Medicine, New Haven, -noC K. NOSWAD (43), Department of ygoloisyhP
tucitcen 01560 and the Cellular and Molecular Biology
R. M. CASE (17), Department of -igoloisyhP ,margorP University of Michigan Medical
cal Sciences, University of ,retsehcnaM ,loohcS Ann ,robrA Michigan .90184
Manchester M13 9PT, England DANIEL C. DEVOR (21), Department of
DEAN CHANG (47), Department of -loisyhP ,ygoloisyhP School of Medicine, State -inU
ogy and the Cellular and Molecular -loiB versity of New York at Buffalo, ,olaffuB
ogy Program, University of Michigan New York 41241
ix
X CONTRIBUTORS TO VOLUME 192
KIERTISIN NROHPAHTASMRAHD (24), -eD B. GmSNER (46), Department of ,ygoloisyhP
partment of Medicine, University of -ilaC University of Wiirzburg, D-8700 -zr~fW
fornia, San Diego, San Diego, ainrofilaC ,grub Federal Republic of ynamreG
30129 L. G. M. GORDON (45), Department of -syhP
P. DmTL (46), Department of ,ygoloisyhP iology, University of Outgo Medical
Dartmouth Medical ,loohcS ,revonaH New School Dunedin, New Zealand
Hampshire 65730 J. GP.AV (32), Department of General and
MICHAEL E. DUFFEY (21), Department of Experimental Pathology, University of
,ygoloisyhP School of Medicine, State -inU ,anneiV ,anneiV Austria
versity of New York at Buffalo, ,olaffuB K. HAG~ (2), Medizinische -statisrevinU
New York 41241 klinik, D-7800 ,grubierF Federal Republic
NILKNARF H. NIETSPE (49), The Mount -seD of Germany
eft Island lacigoloiB ,yrotarobaL Salsbury PHILIPPE A. HALBAN (13, 14), Jeantet Re-
,evoC Maine 27640 hcraes ,seirotarobaL University of aveneG
MICHAEL FIELD (48), Department of Medi- School of Medicine, CH-1211 Geneva ,4
cine and ,ygoloisyhP egelloC of snaicisyhP Switzerland
and ,snoegruS Columbia ,ytisrevinU New JOHN W. HANRAHAN (42), Department of
York, New York 23001 ,ygoloisyhP McGill ,ytisrevinU Montreal
GtlNHAR FLEMSTnOM (9), Department of cebeuQ H3G 1Y6, adanaC
ygoloisyhP and Medical ,scisyhpoiB -ppU WILLIAM R. HARVEY (39, 40), Department
sala University Biomedical Center, S-751 of Biology, Temple University, -ledalihP
32 ,alasppU Sweden ,aihp Pennsylvania 22191
HENRIK LLESSROF (9), Department of Sur- ULRICH HEGEL (29), Institut far Klinische
gery, Sahlgren's Hospital University of ,eigoloisyhP Universitdtsklinikum ,ztilgetS
,grubnehtoG S-143 54 ,grubnehtoG Swe- Freie tatisrevinU ,nilreB D-1000 Berlin ,54
ned Federal Republic of Germany
J. G. FORTE (10), Department of raluceloM HPESOJ HELMAN (3), Department of Oral
and Cell ,ygoloiB University of ,ainrofilaC and Maxillofacial ,yregruS Rambam Med-
,yelekreB Berkeley, ainrofilaC 02749 ical ,retneC Haifa, Israel 69013
LEAHCIM MMORF (29), Institutj~r ehcsinilK NEHPETS HERSEY (8), Department of -isyhP
,eigoloisyhP Universitdtsklinikum ,ztilgetS ,ygolo Emory ,ytisrevinU Atlanta, aigroeG
Freie tatisrevinU ,nilreB 0001-)1 Berlin ,54 22303
Federal Republic of ynamreG ULRICH HOPPER (25), Department of -isyhP
E. FR6MTER (5, 45), Zentrum der -oloisyhP ology and Biophysics, Case Western Re-
,eig Johann Wolfgang ,tdtisrevinU-ehteoG serve ,ytisrevinU ,dnalevelC Ohio 60144
D-6000 Frankfurt am Main, Federal Re- V^LEnlE J. HORN (3), Clinical -agitsevnI
public of Germany tions and Patient Care Branch, National
D. V. GALLACHER (20), Department of Institute ofDentaI ,hcraeseR National lnsti-
,ygoloisyhP University of ,loopreviL Liver- tutes of ,htlaeH ,adsehteB Maryland 29802
pool L69 3BX, England TREBOR T. JENSEN (16, 18), The Digestive
YRREJ D. GARDNER (16, 18), The evitsegiD Diseases Branch, National Institute of
Diseases Branch, National Institute of ,setebaiD Digestive and Kidney ,sesaesiD
,setebaiD Digestive and Kidney ,sesaesiD National Institutes of Health, Bethesda,
National Institutes of Health, Bethesda, Maryland 29802
Maryland 29802 SAMOHT J. HCSTNEJ (37), Zentrumf~r Mo-
HAIM GARTY (44), Department of Mem- lekulare ,eigoloiborueN Universitdt Ham-
brane Research, The Weizmann Institute ,grub D-2000 Hamburg ,02 Federal Re-
of ,ecneicS Rehovot, Israel cilbup of ynamreG
CONTRIBUTORS TO VOLUME 192 xi
NEIL ZTIWOLPAK (30), tnemtrapeD of Medi- RETEP J. MEIER (33, 34), Division of lacinilC
cine, USC School of Medicine, Los An- ,ygolocamrahP Department of ,enicideM
,seleg ainrofilaC 33009 ytisrevinU Hospital CH-8091 Zurich,
GEOROE A. IOMMICH (22), Department of dnalreztiwS
,yrtsimehcoiB School of Medicine and HANS RETE>I MEISSr~R (15), Free -revinU
,yrtsitneD ytisrevinU of ,retsehcoR -sehcoR sity of ,nilreB D-IO00 Berlin ,12 Federal
,ret New York 24641 cilbupeR of ynamreG
ULLA KLEIN (40), sehcsigolooZ lnstitut red SEMAJ E. MELVIN (3), tnemtrapeD of latneD
,tiitisrevinU D-8000 Munchen ,2 Federal ,hcraeseR ytisrevinU of retsehcoR Medical
Republic of ynamreG ,retneC ,retsehcoR New York 24641
H. KNAUF (2), Medizinische -stdtisrevinU DELIA MENOZZl (18), The Digestive Dis-
klinik, D-7800 ,grubierF Federal cilbupeR
sesae Branch, National Institute of Dia-
of ynamreG
,seteb evitsegiD and Kidney ,sesaesiD -aN
G. KOTTRA (45), Zentrum red ,eigoloisyhP tional Institutes of Health, Bethesda,
Klinikum red .J .W ,t'atisrevinU-ehteoG Maryland 29802
6000 Frankfurt ,07 Federal Republic of
ECNERWAL M. MERTZ (3), ygolonircodnE
ynamreG
and noitcudorpeR Research Branch, -aN
NOMIS A. LEWlS (42), Department of -isyhP tional Institute of Child Health and
ygolo and ,scisyhpoiB ytisrevinU of Texas Human ,tnempoleveD National Institutes
Medical ,hcnarB ,notsevlaG Texas 05577 of ,htlaeH ,adsehteB Maryland 29802
CAROLE M. LmDT~ (35), Department of
ASSILEM MILLER (8), Department of -isyhP
,scirtaideP Case Western Reserve -revinU
,ygolo Emory ,ytisrevinU Atlanta, aigroeG
,ytis ,dnalevelC Ohio 60144
22303
YRRET E. MACHEN (4), Department of Mo-
AUSTIN K. MIRCHEFF (23), Department of
ralucel and Cell ,ygoloiB ytisrevinU of -laC
ygoloisyhP and ,scisyhpoiB ytisrevinU of
,ainrofi Berkeley, Berkeley, California
nrehtuoS ,ainrofilaC School of ,enicideM
02749
Los ,selegnA ainrofilaC 33009
SEMAJ L. MADARA (24), Department of -aP
MARK W. MUSCH (48), tnemtrapeD of Med-
,ygoloht Harvard Medical ,loohcS ,notsoB
,enici The ytisrevinU of ,ogacihC ,ogacihC
sttesuhcassaM 51120
Illinois 60637
S. H. P. MADDRELL (41), tnemtrapeD of Zo-
,ygolo University of ,egdirbmaC Cam- PAUL A. NEGULESCU (4), Department of
egdirb 2BC 3EJ, England raluceloM and Cell ,ygoloiB ytisrevinU of
,ainrofilaC Berkeley, ,yelekreB ainrofilaC
ARAWSEMARAP MALATm (27), tnemtrapeD
02749
of ygoloisyhP and ,scisyhpoiB ytisrevinU
of Medicine and yrtsitneD of New Jersey- I. NOVAK (1), Physiologisches lnstitut,
Robert Wood Johnson Medical ,loohcS ,tdtisrevinU-sgiwduL-treblA D-7800 -ierF
,yawatacsiP New Jersey 45880 ,grub Federal Republic of ynamreG
DANUTA H. AKSWONILAM (7), tnemtrapeD SCOTT M. O'GRADY (48), Department of
of ygoloisyhP and ,scisyhpoiB ytisrevinU yranireteV Biology, ytisrevinU of Minne-
of itannicniC egelloC of ,enicideM -nicniC ,atos .tS Paul Minnesota 80155
,itan Ohio 45267 H. RENHTIELREBO (46), Department of
JEAN-PIERRE MAUGER (31), INSERM U Physiology, University of ,grubzrtfW
274, ~tisrevinU Paris-Sud, Orsay, 91405 D-8700 ,grubzrtfW Federal Republic of
,xedeC ecnarF ynamreG
OLOAP MEDA (13, 14), Department of -roM MURAD OOKHTENS (30), Department of
,ygolohp University of aveneG School of ,enicideM CSU School of ,enicideM Los
,enicideM 1121-HC aveneG ,4 Switzerland ,selegnA ainrofilaC 33009
xii CONTRIBUTORS TO VOLUME 192
Y. KUHCPlSO (20), Bogomoletz Institute of versity of New York at Buffalo, ,olaffuB
Physiology, Ukranian Academy of New York 41241
,secneicS 106252 Kiev ,42 U.S.S.R.
DRAHCIR J. NOMOLOS (49), Renal ,tinU
J. A. NOTREVO (41), Department of ,ygolooZ Westchester County Medical Center and
ytisrevinU of ,egdirbmaC Cambridge CB2 New York Medical ,egelloC ,allahlaV New
3E ,J England York 59501
NAIRB E. I~ERCE (11), Department of -isyhP LEINAD D. HTEAPS (39), Department of Bi-
ygolo and ,scisyhpoiB University of Texas ,ygolo Temple University, ,aihpledalihP
Medical ,hcnarB ,notsevlaG Texas 05577 aiavlysnneP 22191
O. H. NESRETEP (20), Department of -isyhP
MARK TAKAHASm (27), Department of
,ygolo University of ,loopreviL Liverpool
Physiology and Biophysics, University of
L69 3BX, England
Medicine and Dentistry of New Jersey-
J. M. SPILLIHP (32), Research Institute and Robert Wood Johnson Medical ,loohcS
Department of Pathology, Hospital for ,yawatacsiP New Jersey 45880
Sick Children and University of ,otnoroT
ARUAL TANG (8), Department of ,ygoloisyhP
,otnoroT ,oiratnO adanaC
Emory ,ytisrevinU Atlanta, aigroeG 22303
NAJ POHL (8), Microchemical Facility,
Emory ,ytisrevinU Atlanta, aigroeG 22303 R. JAraES TURNER (3), Clinical -agitsevnI
tions and Patient Care Branch, National
W. W. ARTSNEER (10), Research Institute,
Institute of Dental Research, Bethesda,
s'nerdlihC Hospital of ,dnalkaO ,dnalkaO
Maryland 29802
ainrofilaC 90649
ELIME J. J. M. NAV NEVROC (23), -trapeD
L. REUSS (43), Department of ygoloisyhP
ment of Physiology and Biophysics, Uni-
and Biophysics, The University of Texas
versity of Southern ,ainrofilaC School of
Medical ,hcnarB ,notsevlaG Texas 05577
Medicine, Los ,selegnA ainrofilaC 33009
OKASUF SATO (38), Department of Derma-
,ygolot University of lowa egelloC of Medi- M. WAKUI (20), Department of ,ygoloisyhP
Tohoku University, School of ,enicideM
,enic Iowa City, Iowa 24225
Sendai 980, Japan
OZNEK OTAS (38), Department of -lotamreD
ogy, University of Iowa College of Medi- NAILUJ R. F. SRETLAW (28), Department of
,enic Iowa City, Iowa 24225 Medicine, Royal Postgraduate Medical
,loohcS Hammersmith Hospital, London
ENERI ZLUHCS (19), Max-Planck-Institut r~if
WI2 0HS, England
,kisyhpoiB D-6000 Frankfurt am Main ,07
Federal Republic of Germany W. WANG (46), Department of ralulleC and
TUMLEH LKIEWHCS (40), Zoologisches In- Molecular Physiology, Yale Medical
stitut der ,tdtisrevinU D-8000 Munchen ,2 ,loohcS New Haven, tucitcennoC 01560
Federal Republic of Germany NEHPETS A. WANK (16), The Digestive Dis-
Y. SEGAL (43), Department of ygoloisyhP eases Branch, National Institute of Dia-
and Biophysics, The University of Texas ,seteb Digestive and Kidney ,sesaesiD Na-
Medical ,hcnarB ,notsevlaG Texas 05577 tional Institutes of Health, Bethesda,
Maryland 29802
G. AZNEMES (26), Department of -simehcoiB
try, Swiss Institute of ,ygolonhceT ETH- F. RENAEW (43), Max-Plank-Institutf~r Sy-
Zentrum, CH-8092 Zurich, Switzerland ,eigoloisyhpmet D-4600 Dortmund ,1 Fed-
OICIRTAP AVLIS (49), Department of Medi- eral Republic of ynamreG
,enic Beth Israel ,latipsoH Boston, Massa- MILTON M. WEISER (28), Department of
sttesuhc 51220 Medicine, State University of New York at
NEVETS M. OKSAMIS (21), Department of ,olaffuB Buffalo lareneG ,latipsoH ,olaffuB
,ygoloisyhP School of Medicine, State -inU New York 30241
CONTRIBUTORS TO VOLUME 192 xiii
HELMUT KEROZCEIW (40), sehcsigolooZ In- ,yrtsi ytisrevinU of aveneG School of Medi-
stitut red ,tdtisrevinU D-8000 Munchen ,2 ,enic 1121-HC aveneG ,4 Switzerland
laredeF Republic of ynamreG TADATAKA YAMADA (12), Department of
MICHAEL WIEDERHOLT (37), Institut fftr Internal ,enicideM ytisrevinU of nagihciM
ehcsinilK ,eigoloisyhP Klinikum ,ztilgetS ,slatipsoH Ann ,robrA Michigan 90184
Freie tdtisrevinU ,nilreB l)-1000 nilreB ,54 SEMAJ R. SAKSAK~rAY (36), Department of
laredeF Republic of ynamreG ,enicideM The ytisrevinU of North -oraC
MICHAEL G. REGREBSREFLOW (40), -trapeD lina at Chapel Hill, Chapel Hill, North
ment of ,ygoloiB Temple ,ytisrevinU -alihP aniloraC 99572
,aihpled ainavlysnneP 22191 D, YULE (20), Department of ,ygoloisyhP
SEALC B. WOLLHEIM (13, 14), tnemtrapeD ytisrevinU of ,loopreviL Liverpool L69
of ,enicideM Division of lacinilC -mehcoiB 3BX, England
Preface
Biological transport is part of the Biomembranes series of Methods in
Enzymology. It is a continuation of methodology concerned with mem-
brane function. This is a particularly good time to cover the topic of
biological membrane transport because there is now a strong conceptual
basis for its understanding. The field of transport has been subdivided into
five topics.
.1 Transport in Bacteria, Mitochondria, and Chloroplasts
2. ATP-Driven Pumps and Related Transport
.3 General Methodology of Cellular and Subeellular Transport
4. Cellular and Subcellular Transport: Eukaryotic (Nonepithelial) Cells
5. Cellular and Subcellular Transport: Epithelial Cells
Topic l covered in Volumes 521 and 126 initiated the series. Topic 2 is
covered in Volumes 651 and 157, Topic 3 in Volumes 171 and 172, and
Topic 4 in Volumes 371 and 174. The remaining Topic 5 is now covered in
191 and 192.
Topic 5 is divided into two parts: this volume (Part W) which deals with
gastrointestinal and a diversity of other epithelial cells, and Volume 191
(Part V) which covers transport in kidney, hormonal modulation, stimulus
secretion coupling, pharmacological agents and targeting, and intracellular
trafficking in epithelial cells.
We are fortunate to have the good counsel of our Advisory Board.
Their input insures the quality of these volumes. The same Advisory Board
has served for the complete transport series. Valuable input on the outlines
of the five topics was also provided by Qais A1-Awqati, Ernesto Carafoli,
Halvor Christensen, Isadore Edelman, Joseph Hoffman, Phil Knauf, and
Hermann Passow. Additional valuable input for Volumes 191 and 291 was
obtained from Michael Berddge, Eberhard Fromter, Ari Helenius, and
Heine Murer.
The names of our advisory board members were inadvertently omitted
in Volumes 521 and 126. When we noted the omission, it was too late to
rectify the problem. For volumes 521 and 126, we are also pleased to
acknowledge the advice of Angelo Azzi, Youssef Hatefi, Dieter Oesterhelt,
and Peter Pedersen.
The enthusiasm and cooperation of the participants have enriched and
made these volumes possible. The friendly cooperation of the staff of
Academic Press is gratefully acknowledged. We are pleased to acknowledge
Ms. Laura Taylor for her tireless efforts and secretarial skills.
XV
x~ ECAFERP
These volumes are dedicated to Professor Sidney Colowick, a dear
friend and colleague, who died in 1985. We shall miss his wise counsel,
encouragement, and friendship.
YENDIS REHCSIELF
ACCEB REHCSIELF
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV II. Preparation and Assay of Enzymes
by Edited YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
Edited yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV IV. Special Techniques for the Enzymologist
by Edited YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV .V Preparation and Assay of Enzymes
Edited yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
by Edited YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VII. Cumulative Subject Index
by Edited YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VIII. ComplexC arbohydrates
Edited by HTEBAZILE F. DLEFUEN DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by SILLIW A. DOOW
EMULOV X. Oxidation and Phosphorylation
by Edited DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
by Edited C. H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and )B
by Edited ECNERWAL NAMSSORG DNA EIVIK EVADLOM
iivx
xviii METHODS IN ENZYMOLOGY
EMULOV XIII. Citric Acid Cycle
detidE yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
detidE yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpcnoids
detidE yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
detidE yb HTENNEK NITSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
detidE yb TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
detidE yb DLANOD B. McCoRMICK DNA LEUMEL O. THGIRW
EMULOV XIX. Proteolytic Enzymes
detidE yb EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
detidE yb KIVlE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
detidE yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
detidE yb MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
detidE yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
detidE yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT