Biomembranes Part F: Bioenergetics: Oxidative Phosphorylation

958 Pages·1979·13.074 MB·English

by  Sidney Fleischer, Lester Packer (Eds.)

Contributors ot Volume LV
elcitrA numbers are in parentheses following the names of contributors.
Affiliations listed are current.
ADOLFO ALEXANDRE (70), Department of imie, Department de Recherche Fonda-
Physiological Chemistry, Johns Hopkins mentale, Centre d'Etudes Nucleaires,
University School of Medicine, Bal- 85X 14083 Grenoble-cedex, France
timore, Maryland 50212
ARNOLD F. BROD1E (22), Department of
L. MARIO AMZEL (39), Department of Bio- Biochemistry, University of Southern
physics, Johns Hopkins University School California School of Medicine, Los An-
of Medicine, Baltimore, Maryland 50212 geles, California 33009
DANIEL E. ATKINSON (25), Biochemistry BARBARA CANNON (1, 8), The Wenner-Gren
Institute, University of Stockholm, S-113
Division, Department of Chemistry, Uni-
54 Stockholm, Sweden
versity of California, Los Angeles, Cali-
fornia 42009 CHRISTINE CARLSSON (51), Department of
Biochemistry, Arrhenius Laboratory,
GIOVANNI FELICE AZZONE (5), (2) C.N.R.
University of Stockholm, S-106 19 Stock-
Unit for the Study of Physiology of -iM
holm, Sweden
tochondria, Institute of General Pathol-
ogy, University of Padova, Padova, Italy WILLIAM A. CATTERALL (30, 37), Depart-
ment of Pharmacology, University of
C. L. BASHFORD (65), Johnson Research
Washington School of Medicine, Seattle,
Foundation, University of Pennsylvania,
Washington 50189
Philadelphia, Pennsylvania 40191
R. B. BEECHEY (40, 55, 59), Shell Research J. CHABERT (60), Laboratoire de Biochimie,
Departement de Recherche Fondamen-
Limited, Shell Biosciences Laboratory,
tale, Centre d'Etudes Nucleaires, 85X
Sittingbourne Research Centre, Sitting-
14083 Grenoble-cedex, France
bourne, Kent ME9 8AG, England
ASTRID G. CHAPMAN (25), Biochemistry
BRUNO BLANCHY (80), Laboratoire de Biol-
Division, Department of Chemistry, Uni-
ogie et Technologie, des Membranes
versity of California, Los Angeles, Cali-
(LBTM)-CNRS, Universitd Claude Ber-
fornia 42009
nard de Lyon, 69621 Villeurbanne,
France R. CHmOVSKY (43), Department of Biology,
Technion-lsrael Institute of Technology,
PAUL O. BOYER (27), Molecular Biology In-
Haifa, Israel
stitute, Department of Chemistry, -revinU
sity of California, Los Angeles, Califorma NITZA M. CINTR6N (52), Laboratory of
Molecular and Cellular Bioenergetics,
P. D. BRAGG (85), Department of Biochem-
Department of Physiological Chemistry,
istry, University of British Columbia,
Johns Hopldns University School of Med-
Vancouver, British Columbia, Canada
icine, Baltimore, Maryland 50212
V6T 5W1
MARTIN D. BRAND (70), Department of Bio-
J. B. CLARK (6), Department of Biochem-
chemistry, University of Cambridge,
istry, St. Bartholomew's Hospital Medi-
Cambridge 2BC 1QW, England
cal, College, University of London,
G. BRANDOLIN (60), Laboratoire ed Bioch- London EC1M 6BQ, England
XV
xvi
CONTRIBUTORS TO VOLUME LV
DLANOR S. COCKRELL (72), Edward A. de Biologie et Technologie, des Mem-
Doisy Department of Biochemistry, St. branes (LBTM)-CNRS, Universit6 Claude
Louis University School of Medicine, St. Bernard de Lyon, 69621 Villeurbanne,
Louis, Missouri 40136 France
NATALIE S. COHEN (22), Department of JOHN W. TLAWANEERG (10), Department of
Biochemistry, University of Southern Biochemistry, University of Tennessee,
California School of Medicine, Los An- Knoxville, Tennessee 61973
geles, California 33009
BERNARD GUgRIN (19), Laboratoire de
RAFFAELE COLONNA (5), (2) C.N.R. Unit Biochemie, Universite de Bordeaux H,
50433 Talence, France
for the Study of Physiology of Mitochon-
dria, Institute of General Pathology, F. GUERmERI (68), Universita di Bari, ln-
University of Padova, Padova, Italy stituto di Chimica Biologica, Facolta di
Medicina e Chirurgia, 70126 Bari, Italy
JANE COMTE (11), Laboratoire de Biologie
et Technologie des Membranes LBTM-- D. L. GUTNJCK (43), Department of Micro-
CNRS, Universite Claude Bernard de biology, George Wise Center for Life Sci-
Lyon, 69621 Villeurbanne, France ences, Tel Aviv University, Ramat Aviv,
Israel
ARABRAB E. CORKEY (23), Department of
Biochemistry and Biophysics, University YOUSSEF HATEFI (9, 34), Department of
of Pennsylvania School of Medicine, Phil- Biochemistry, Scripps Clinic and Re-
adelphia, Pennsylvania 40191 search Foundation, La Jolla, California
73029
DRAHCIR ELDD1RC (53), Niigata College of
Pharmacy, 5829 Kamishin-ei, Niigata WALTER P. HEMPFLINO (21), Department
,12-059 Japan of Biology, University of Rochester,
Rochester, New York 72641
LARS ERNSTER (51), Department of Bio-
chemistry, Arrhenius Laboratory, Uni-
ELLIOT L. GREBZTREH (21), Department of
versity of Stockholm, S-106 9l Stockholm,
Cell Biology, ehT Rockefeller University,
Sweden
New York, New York 12001
R. H. F1LLINGAME (55), Department of
PETER G. HEYTLER (58), Central Research
Physiological Chemistry, University of
and Development Department, Experi-
Wisconsin Medical School, Madison,
mental Station, E. I. Du Pont de Nemours
Wisconsin 60735
& Company, Wilmington, Delaware
SIDNEY FLEISCHER (2, 3, 87), Department 89891
of Molecular Biology, Vanderbilt Univer-
PETER C. HINKLE (63, 81), Section of Bio-
sity, Nashville, Tennessee 53273
chemistry, Molecular, and Cell Biology,
Y. M. GALANTE (34), Department of Bio- Cornell University, Ithaca, New York
chemistry, Scripps Clinic and Research 35841
Foundation, La Jolla, California 92037
EM1JAH HIRATA (73), Department of Bio-
EL~IINAD C. NOREHTUAG (11, ,31 80), La- chemistry, Jichi Medical School, Tochigi,
boratoire de Biologie et Technologie, des Japan 40-923
Membranes (LBTM) -CNRS, Universitd
Bo HOJEBERG (29), Department of Bio-
Claude Bernard de Lyon. 69621 Villeur-
chemistry, Arrhenius Laboratory, Uni-
banne, France
versity of Stockholm, S-106 19 Stockholm,
E4rIREtrTAC GODINOT (13, 80), Laboratoire Sweden
CONTRIBUTORS TO VOLUME LV xvii
JOANNE HULLIHEN (79), Laboratory for cology, Biochemistry, and Biophysics,
Molecular and Cellular Bioenergetics, The Cardiovascular Research Institute,
Department of Physiological Chemistry, University of California School of Medi-
Johns Hopkins University School of Med- cine, San Francisco, California 34149
icine, Baltimore, Maryland 50212
PIERRE LABBE (19), Laboratoire de Bioch-
TORILL HUNDAL (51), Department of Bio- imie des Porphyrines, Universite de Paris
chemistry, Arrhenius Laboratory, Uni- VII, Tour ,34 50057 Paris, France
versity of Stockholm, S-106 19 Stockholm,
J. C. K. LAI (6), Miriam Marks Department
Sweden
of Neurochemistry, Institute of Neurol-
SAgOJ Josnl (47, 48), Department of Cell ogy (Queen's Square), University of Lon-
Physiology, Boston Biomedical Research don, London WCIM 3BQ, England
Institute, Boston, Massachusetts 02114
DAVID O. LAMBETH (37), Department of
H. RONALD KABACK (74), The Roche Insti- Biochemistry, University of North Da-
tute of Molecular Biology, Nutley, New kota, Grand Forks, North Dakota 20285
Jersey 01170
JANOS LANYI (67), NASA Ames Research
YASUO KAGAWA (44, 45, 77, 83, 84), De- Center, Moffett Field, California 53049
partment of Biochemistry, Jichi Medical
School, Japan 40-923 HENRY A. LARDY (37), The Institute of En-
zyme Research, The University of Wis-
VIJAY K. KALRA (22), Department of Bio- consin, Madison, Wisconsin 60735
chemistry, University of Southern Cali-
fornia School of Medicine, Los Angeles, G. J. M. LAUQUIN (60), Laboratoire de
California 33009 Biochimie, Department ed Recherche
Fondamentale, Centre d'Etudes Nu-
R. M. KASCHNITZ (9), Institute for Molec- cleaires, 38041 Grenoble-cedex, France
ular Biology, Austrian Academy of Sci-
ences, A-5020 Salzburg, Austria Soor~-Ho LEE (22), Department of Bio-
chemistry, University of Southern Cali-
Tsoo E. KING (15), Laboratory of Bioener-
fornia School of Medicine, Los Angeles,
getics and Department of Chemistry, The
California 33009
State University of New York Albany,
New York 22221 CHUAN-Pu LEE (12), Department of Bio-
chemistry, Wayne State University School
EVA KIRSTEN (14), Departments of Phar-
of Medicine, Detroit, Michigan 10284
macology, Biochemistry, and Biophysics,
The Cardiovascular Research Institute, ALBERT L. LEHNINGER (70), Department of
University of California School of Medi- Physiological Chemistry, Johns Hopkins
cine, San Francisco, California 34149 University School of Medicine, Balti-
more, Maryland 50212
GgRARD KLEIN (54), Laboratoire de Bioch-
imie, Department ed Recherche Fonda- OLOV LINDBERG (8), The Wenner-Gren In-
mentale, Centre d'Etudes Nucleaires, stitute, University of Stockholm, S-113 54
14083 Grenoble-cedex, France Stockholm, Sweden
TETSUYA KONISHI (53), Membrane Bio- P. E. LINNETT (40, 55, 59), Shell Research
energetics Group, Department of Physi- Limited, Shell Biosciences, Laboratory,
ology-Anatomy, University of California, Sittingbourne Research Centre, Sitting-
Berkeley, California 02759 bourne, Kent ME9 8AG, England
ERNEST KUN (14), Department of Pharma- DAVID LLOYD (17), Department of Micro-
XVIII CONTRIBUTORS TO VOLUME LV
biology, University College, Card(ff 2FC ology-Anatomy, University of California
1 TA, Wales at Berkeley, Berkeley, California 95720
J. OLIVER MCINTYRE (3), Department of S. PAPA (68), Universitd di Bari, lstituto di
Molecular Biology, Vanderbilt Univer- Chimica Biologica, Facolta di Medicini e
sity, Nashville, Tennessee 53273 Chirurgia, 70126 Bari, Italy
LEENA MELA (4), Departments of Surger) M. D. PARTIS (40), Department of Molecu-
and Biochemistry and Biophysics, Uni- lar Sciences, University of Warwick, Cov-
versity of Pennsylvania School of Medi- entry CV4 7AL, United Kingdom
cine, Philadelphia, Pennsylvania 19104
PETER L. PEDERSEN (9, 30, 37, 38, 39, 52,
A. D. MITCHELL (40), Shell Research Lim- 79), Laboratory for Molecular and Cel-
ited, Shell Biosciences Laboratory, Sit- lular Bioenergetics, Department of Phy-
tingbourne Research Centre, Sitting- siological Chemistry, Johns Hopkins
bourne, Kent ME9 SAG, England University School of Medicine, Balti-
more, Maryland 50212
PETER MITCHELL (69), Glynn Research
Laboratories, Bodmin, Cornwall PL30 YEVRAH S. YKSFENEP (32, 33, 46), The Pub-
4A ,U England cil Health Research Institute, The City of
New York, Inc., New York, New York
RoY MITCHELL (69), Glynn Research Lab- 61001
oratories, Bodmin, Cornwall PL30 4A ,U
England WALTER N. PIPER (14), Department of
Pharmacology, University of California
DLORAH P. MORRIS (9), Department of Bio- School of Medicine, San Francisco, Cal-
chemistry, Howard University School of ifornia 34149
Medicine, Washington, D.C. 20059
SELRAHC A. PLATE (61), Department of Bi-
JENNIFER MOYLE (69), Glynn Research ology, Massachusetts Institute of Tech-
Laboratories, Bodmin, Cornwall PL30 nology, Cambridge, Massachusetts 93120
4A ,U England
BRIAN POOLE (16), Department of Pathol-
JAN NEDERGAARD (1), The Wenner-Gren ogy, Mt. Sinai School of Medicine, The
Institute, Universi.ty of Stockholm, S-113 Rockefeller University, New York, New
45 Stockholm, Sweden York 12001
N. NELSON (43), Department of Biology, NOTREB C. NAMSSERP (72), Department of
Technion-Israel Institute of Technology, Pharmacology, School of Medicine, Uni-
Haifa, Israel versity of Miami, Miami, Florida 25133
WALTER NEUPERT (18), lnstitut fiir Physil-
ogische Chemic, Physikalische Biochemie EFRAIM RACKER (35, 50, 76), Department
und Zellbiologie der Universitiit Munchen, of Biochemistry, Molecular and Cell -iB
8 Munchen 2, Federal Republic of Ger- ology, Corneli University, Ithaca, New
many York 35841
KERSTIN DNARBNEDRON (51), Department C. IAN RAGAN (78), Department of Bio-
of Biochemistry, Arrhenius Laboratory, chemistry, School of Biochemical and
University of Stockholm, S-106 19 Stock- Physiological Sciences, ehT University of
holm, Sweden Southampton, Southampton, S09 3TU,
England
LESTER PACKER (53, 67), Membrane Bio-
energetics Group, Department of Physi- SOFIA RAMOS (74), National Institute of
CONTRIBUTORS TO VOLUME LV xix
Arthritis and Metabolism, and Digestive STEVEN SEITZ (4), Departments of Surgery
Diseases, National Institutes of Health, and of Biochemistry and Biophysics,
Bethesda, Maryland 41002 University of Pennsylvania School of
Medicine, Philadelphia, Pennsylvania
PETER W. REED (56), Department of Phar-
40191
macology, School of Medicine, Vanderbilt
University, Nashville, Tennessee 37232 A. E. SENIOR (49), Department of Biochem-
istry, University of Rochester Medical
BALTAZAR REYNAFARJE (70), Department
Center, Rochester, New York 24641
of Physiological Chemistry, Johns Hop-
kins University School of Medicine, Bal- PAUL K. SHIEH (67), Membrane Bioener-
timore, Maryland 50212 getics Group, University of California,
Berkeley, California 02749
L. ROSSI-BERNARDI (68), Cattedra di Chim-
ica Biologica, Universita di Milano, THOMAS P. SINGER (57), Molecular Biology
Milan, Italy Division, Veterans Administration Hos-
pital, San Francisco, California 12149
HAGAI ROTTENBERG (64), Bell Laborato-
ries, Murray Hill, New Jersey 47970 V. P. SKULACHEV (66, 82), Department of
Bioenergetics, A. N. Belozersky Labora-
JAN RYDSTR6M (28, 29, 87), Department of
tory of Molecular Biology and Bioorganic
Biochemistry, Arrhenius Laboratory,
Chemistry, Moscow State University,
University of Stockholm, S-106 19 Stock-
Moscow, USSR
holm, Sweden
E. C. SEATER (26), Laboratory of Biochem-
D. RAO SANADI (47), Department of Biolog-
istry, B. .C P. Jansen Institute, University
ical Chemistry, Harvard Medical School,
of Amsterdam, Amsterdam-C, ehT
Boston, Massachusetts 51120
Netherlands
R. RAO SANADI (48), Boston Biomedical
CLIFFORD L. SEAYMAN (71), Departments
Research Institute, Boston, Massachu-
of Physiology and Human Genetics, Yale
setts 51120
University School of Medicine, New
MICHEL SATRE (54), Laboratoire de Bioch- Haven, Connecticut 01560
imie, Department de Recherche Fonda-
CAROLYN W. SEAYMAN (71), Departments
mentale, Centre d'Eludes Nueleaires,
of Physiology and Human Genetics, Yale
85X 14083 Grenoble-cedex, France
University School of Medicine, New
A. SCARPA (7), Department of Biochemistry Haven, Connecticut 01560
and Biophysics, ehT Pennsylvania Muscle
B. SLOANE (7), Department of Biochemistry
Institute, University of Pennsylvania
and Biophysics, ehT Pennsylvania Muscle
School of Medicine, Philadelphia, Penn-
Institute, University of Pennsylvania
sylvania 40191
School of Medicine, Philadelphia, Penn-
SHIMON SCHULDINER (74), Department of sylvania 40191
Molecular Biology, The Hebrew Univer-
J. C. SMITH (65), Johnson Research Foun-
sity, Hadassah Medical School, Jerusa-
dation, University of Pennsylvania, Phil-
lem, Israel
adelphia, Pennsylvania 40191
WALTER SEBALD (18, 41), Gesellschaft r~if
MARIE SOMLO (19), Centre de Genetique
Biotechnologische Forschung mbH. Abtlg.
Moleculaire, Gif ,09119 France
Stoffwechselregulation, 3300 Braun-
schweig-Stockheim, Federal Republic of A. P. SOMLYO (7), Departments of Physi-
Germany ology and Pathology, ehT Pennsylvania
XX CONTRIBUTORS TO VOLUME LV
Muscle Institute, Presbyterian- University PIERRE V. VIGNAIS (54), Laboratoire de
of Pennsylvania Medical Center, Phila- Biochimie, Departement de Recherche
delphia, Pennsylvania 40191 Fondamentale, Centre d'Etudes Nu-
cleaires, 14083-F Grenoble-cedex, France
NOBUHITO SONE (44), Department of Bio-
chemistry, Jichi Medical School, Tochigi, A. D. VINOGRADOV (15), Department of
Japan 40-923 Biology, Moscow State University, Mos-
cow, USSR
JOHN W. SUPER (38), Department of Phy-
siological Chemistry, Johns Hopkins RETN2~G VOOEL (36, 86), Max-Planck-lnsti-
University School of Medicine, Balti- tut fiir Biologie, 47D Tiibingen, Federal
more, Maryland 50212 Republic of Germany
KERSTIN STEMPEL (27), Molecular Biology ALAN S. WAC~ONER (75), Department of
Institute, Department of Chemistry, Uni- Chemistry, Amherst College, Amherst,
versity of California, Los Angeles, Cali- Massachusetts 20010
fornia 42009
JuI H. WANG (62), Bioenergetics Labora-
D. L. STIGGALL (34), Chemistry Depart- tory, State University of New York at Buf-
ment, San Diego State University, San falo, Buffalo, New York 41241
Diego, California 28129
HANNS WEISS (18), European Molecular
ALEXANDER S. SUN (16), Department of Biology Laboratory, 6900 Heidelberg,
Pathology, Mt. Sinai School of Medicine, Federal Republic of Germany
ehT Rockefeller University, New York,
SELRAHC E. WENNER (31), Department of
New York 12001
Experimental Biology, Roswell Park
M. KAYE TREMRATH (20), Department of Memorial Institute, Buffalo, New York
Biochemistry, Monash University, Clay- 36241
ton, Australia
A. H. C. A. WIECHMANN (24), Universiteit
ALEXANDER TZAGOLOFF (20, 42), Public van Amsterdam, Laboratorium your
Health Research Institute, The City of Biochemie, B. .C P. Jansen lnstit, Am-
New York, Inc., New York, New York sterdam-C., ehT Netherlands
61001
GERD WILD (41), lnstitutfiir Physiologische
J. SER~IILLAV (7), Department of Physiol- Chemie und Physikalische Biochemie der
ogy, School of Medicine, Laval. Univer- Universitiit, 8000 Munchen ,2 Federal
sity, Quebec, Canada Republic of Germany
K. VAN DAM (24), Universiteit van Amster- JOHN R. WILLIAMSON (23), Department of
dam, Laboratorium voor Biochemie, B. Biochemistry and Biophysics, University
.C P. Jansen lnstitut, Amsterdam-C., ehT of Pennsylvania School of Medicine, Phil-
Netherlands adelphia, Pennsylvania 40191
JUAN C. VIDAL (3), Facultad de Medicina, EKUSASAM YOSHIDA (84), Department of
lnstituto de Bioquimica, Buenos Aires, Biochemistry, Jichi Medical School, -oT
Argentina chigi, Japan 40-923
PAULETTE M. VIGNAIS (60), Laboratoire de BRUNO ZICHE (5), C.N.R. Unit for the
Biochimie,Ddpartment de -noFlehcre,hceR Study of Physiology, and Mitochondria,
damentale et ERA-CNRS, 14083-F Gren- Institute of General Pathology, Univer-
oble-cedex, France sity of Padova, Padova, Italy
Preface
A great deal of progress has taken place in biological oxidations and
bioenergetics since Oxidation and Phosphorylation edited by Ronald
W. Estabrook and Maynard E. Pullman (Volume X of Methods in
Enzymology) became available in 1967. To update the material origi-
nally treated in this volume, five volumes on biomembranes (Volumes
LII-LVI, Parts C-G, respectively) have been prepared, three dealing
with biological oxidations and two with bioenergetics.
In this volume, Part F of "Biomembranes," subtitled "Bioenergetics-
Oxidative Phosphorylation," we bring together new methodology on
the preparation of organelles, membranes, ATP synthetase complexes,
and purified components relevant to the subject; the measurement of
ATP synthesis and regulation; the measurement of transmembrane po-
tential and intramitochondrial pH; and the dissociation and reconsti-
tution of energy-transducing membranes.
We are pleased to acknowledge the good counsel of the members of
our Advisory Board for these five volumes. Special thanks are also due
Drs. E. Carafoli, G. Palmer, H. Penefsky, and A. Scarpa for their
helpful comments on our outlines for these volumes. Valuable counsel
for this volume was also provided by Drs. D. E. Green, F. Harold, P.
Hinkle, R. Kaback, P. Pedersen, E. Racker, D. R. Sanadi, C. Schnait-
man, T. P. Singer, W. Stoeckenius, A. Tzagoloff, and J. R. Williamson.
We were very gratified by the enthusiasm and cooperation of the par-
ticipants in the field of biological oxidations and bioenergetics whose
advice, comments, and contributions have enriched and made possible
these volumes. The friendly cooperation of the staff of Academic Press
is gratefully acknowledged.
SIDNEY FLEISCHER
LESTER PACKER
ixx
METHODS IN ENZYMOLOGY
DETIDE YB
Sidney P. Colowick and Nathan 0. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation of Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
iiixx
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick Nathan O. Kaplan
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb WILLIS A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XlI. Nucleic Acids (Parts A and B)
Edited by LAWRENCE NAMSSORG DNA KIVlE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. CLAYTON
EMULOV XVI. Fast Reactions
Edited by KENNETH KUSTIN
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb HERBERT ROBAT DNA CELIA WHITE ROBAT
EMULOV XVlII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DONALD B. KCIMROCCM DNA LEMUEL D. WRIGHT
EMULOV XIX. Proteolytic Enzymes
Edited yb GERTRUDE E. NNAMLREP DNA LASZLO DNAROL
VXX
xxvi METHODS IN ENZYMOLOGY
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXlI. Enzyme Purification and Related Techniques
Edited by WILLIAM B. YBOKAJ
EMULOV XXlII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by SIDNEY REHCSIELF DNA LESTER REKCAP
EMULOV XXXlI. Biomembranes (Part B)
Edited by SIDNEY REHCSIELF DNA LESTER REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. DENNIS DNA EDWARD A. SINNED