Bioluminescence Cherniluminescence Progress and Perspectives TThhiiss ppaaggee iinntteennttiioonnaallllyy lleefftt bbllaannkk Pacific0 Yokohama, Yokohama, Japan 2 - 6 August 2004 ISBC 2004 Bioluminescence Chemiluminescence Progress and Perspectives edited by Akio Tsuji Showa University, Japan Masakatsu Matsumoto Kanagawa University, Japan Masako Maeda Showa University, Japan Larry J I<ricka University of Pennsylvania School of Medicine, USA Philip E Stanley Cambridge Research &Technology Transfer Ltd, U I< vp World Scientific NEW JERSEY * LONDON SINGAPORE * BElJlNG * SHANGHAI HONG KONG TAIPEI CHENNAI Published by World Scientific Publishing Co. Re. Ltd. 5 Toh Tuck Link, Singapore 596224 USA office: 27 Warren Street, Suite 401-402, Hackensack, NJ 07601 UK office: 57 Shelton Street, Covent Garden, London WC2H 9HE British Library Cataloguing-in-PublicationD ata A catalogue record for this book is available from the British Library. Proceedings of the 13th International Symposium on BIOLUMINESCENCE AND CHEMILUMINESCENCE Progress and Perspectives Copyright 0 2005 by World Scientific Publishing Co. Re. Ltd. All rights reserved. This book, or parts thereof; may not be reproduced in any form or by any means, electronic or mechanical, including photocopying, recording or any information storage and retrieval system now known or to be invented, without written permission from the Publisher. For photocopying of material in this volume, please pay a copying fee through the Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923, USA. In this case permission to photocopy is not required from the publisher. ISBN 981-256-118-8 Printed in Singapore. WELCOME! On behalf of the Organizing Committee of 13th International Symposium on Bioluminescence & Chemiluminescence 2004 in Yokohama (13th ISBC), I would like to welcome you to the Symposium. The fist symposium was held in 1978 in Brussels, Belgium and the symposium has subsequently been held every two years in Europe and America. Tlus symposium is the first to be held in Japan, as well as in the Asia region. At the first day, the symposium begins with the Memorial Lecture of Late Professor Toshio Goto of Nagoya University presented by Professor H. Niwa, The University of Electro- Communications, and following the Plenary Lectures of Professor 0. Shimomura, Marine Biology Laboratory and Professor W. Adam, the University of Puerto Rico. The plenary lectures will presented by Professor T. Nagano, the University of Tokyo, Professor of F. Tsuji, the University of California, and Professor Y. Umezawa, the University of Tokyo at the second to third day, respectively. These plenary lectures will be followed by symposium sessions on Mechanism and Theoretical Study of Chemiluminescent Reactions, Color of Chemi- and Bioluminescence, Luminescent Bio-imaging and Biosensors developed with Nanomolecules and Nanoparticles, Recent Development in Environmental Fields, Biological Application with Fluorescent Biomolecules and Cells, Bioluminescence; Evolutional, Biological and Ecological Aspects, Synthesis of Compounds related to Chemiluminescence, Basic and Applied Studies of Beatle Bioluminescence, Development in Biological Fields, Frontiers of Industrial Application of Firefly Luciferase, Basic and Applied Studies of Marine and Bacterial Bioluminescence, Functional Chemi-Luminophore and Singlet Oxygen and Superoxide Bioluminescence Chemiluminescence Festival Plaza will be planed for free talk and discussion. Moreover, the education session “Firefly Sheds Light on New Technology” will be opened to the early teens as scientific education. Poster presentations which cover and wide may of topics and expand on information presented during the oral sessions are the Exhibit Hall. In addition, the Exhibit Hall is full of the latest technology to make OUT studies. We hope you will take time to visit each of our exhibitors during the symposium. Bus tour to Hakkeijima Sea Paradise (Aquarium) or Walking tour around historical town Kamakura or Scientific visit to Yokosuka City Museum is prepared to all participants. All attending owe much to the diligent Program Committee and to the Session Chairs who powerful assisted in attracting keynote speakers and in giving logical organization to the submitted papers. Our generous sponsors, listed elsewhere, improved the quality of the amenities of the meeting. On behalf of the entice organizing committee - welcome. We are glad you are here. We hope you will enjoy the next few days and that you have a valuable and rewarding learning experience as well. Akio Tsuji, PhD The President of 13th International Symposium on Bioluminescence and Chemiluminescence TThhiiss ppaaggee iinntteennttiioonnaallllyy lleefftt bbllaannkk PREFACE These are the Proceedings of the 13* Symposium on Bioluminescence and Chemiluminescence held at Pacific0 Yokohama, Yokohama, Japan from 2 - 6 August 2004. This series of symposia started in Brussels in 1978, and a list of the other Proceedings volumes appears at the end of this Preface. As in previous symposia, delegates came from far and wide and in all 18 countries were represented. Communications between delegates and the Organising Secretariat was almost entirely via the Internet and email. In the interest of efficiency and the environment very little printed paperwork was used. Abstracts of presentations were made available on the web site wwwl0.showa-u.ac.ip/-ISBC/index.html. The Organising Secretariat was fortunate to have the continued association with the International Society for Bioluminescence & Chemiluminescence (http://www.unibo.it/isbc). The International Society recognised the need to encourage young scientists in the disciplines and so it provided financial support for 11 young scientists under the age of 35 years We also thank John Wiley & Sons for publishing the regular abstracts in the journal Luminescence Vol. 19(3) 2004. Editorial Note This volume was compiled without peer review from camera-ready manuscripts of lectures and posters presented at the Symposium. The Editors have, in the interest of rapid publication, made only minor stylistic changes. They take no responsibility for scientific or priority matters. The Editors: A Tsuji, M Matsumoto, M Maeda, LJ Kricka, PE Stanley vii viii Preface THE MARLENE DELUCA PRIZES These prizes were again generously given by Dr Fritz Berthold with his new company Berthold Technologies (http://www.bertholdtech.com). Dr Berthold has provided these prizes at each symposium since the 1988 Symposium in Florence. The prizes are open to all Symposium participants who are aged below 35 years by the first day of the Symposium. They are in memory of Marlene DeLuca who made such a major contribution to the science of bioluminescence (see Stanley PE. Dedication to Marlene DeLuca: J Biolumin Chemilum 4;1989:7-11 (includes list of her papers)). The President of the International Society, Professor John Lee (University of Georgia, Athens, GA, USA) chose a small Committee from the Society to judge the presentations. The Prize winners were: Akira Kanakubo (Laboratory of Organic Chemistry, School of Bioagricultural Sciences, Nagoya Univeristy, Nagoya, Japan) Chemical studies on bioluminescence of Acorn worm, Ptychodera flava: Isolation and characterization of luminous substances Asami Kaihara (Department of Chemistry, School of Science, University of Tokyo, Tokyo, Japan) Flashing a protein-protein interaction in living cells by a split Renilla luciferase complementation Christine Vanderlinden (Laboratoire de Biologie Marine, Universitk Catholique de Louvain, Louvain-la-Neuve, Belgium) Pharmacological and electrophysiological studies of light emission in 3 ophiuroid species: preliminary results Preface ix ORGANIZATION PRESIDENT A Tsuji VICE PRESIDENTS M. Aizawa and M. Matsumoto GENERAL SECRETARY M Maeda INTERNATIONAL ADVISORY BOARD ISBC Council Members J. Lee (President), A. Roda (Past President), A. Szalay (President-Elect), P. Pasini (Secretary), P.E. Stanley (Past Secretary), B. Branchini (Treasurer & Membership Secretary), W Kricka (Publication Officer) Councillors A. BerthoId , S. Haddock, P. Hill, V. Kratasyuk, J-Francois Rees, E. Widder, B. Zomer Scientific Advisory Board S. Daunert, P. De Sole, T. Quickenden, P. Schaap, D. Shah, N Ugarova, V. Viviani, Xiaolin Yang and also Jin-Min Lin, Shiao-Chun (David) Tu NATIONAL ADVISORY BOARD J. Goto, T. Masujima, T. Nagano, M. Ohashi, Y. Umezawa, H. Utsumi SCIENTIFIC PROGRAM AND LOCAL ORGANIZING COMMITTEE A. Tsuji (President), M. Aizawa (Vice President), M. Matsumoto (Vice President), M. Maeda (General Secretary), N. Amino, Y. Ashihara, H. Arakawa, K. Fujimori, M. Fukuoka, S. Harada, N. Hattori, K. Hayakawa, M. Hiramatsu, S. Hosaka, H. Hosoda, I. Imada, K. Imai, M. Inoue, S. Inouye, M. Isobe, Y. Kasahara, M. Kimura, N. Kuroda, K. Nakashima, H. Niwa, 0. Nozaki, K. Ohmiya, N. Suzuki, M. Totani, T. Toyooka, K. Tsujimoto, N. Wada, M. Yamaguchi