ebook img

Biological NMR Spectroscopy - J. Markley, S. Opella (Oxford, 1997) WW PDF

375 Pages·1997·25.03 MB·English
by  
Save to my drive
Quick download
Download
Most books are stored in the elastic cloud where traffic is expensive. For this reason, we have a limit on daily download.

Preview Biological NMR Spectroscopy - J. Markley, S. Opella (Oxford, 1997) WW

BIOLOGICAL NMR SPECTROSCOPY This page intentionally left blank BIOLOGICAL NMR SPECTROSCOPY Edited by John L. Markley University of Wisconsin, Madison Stanley J. Opella University of Pennsylvania New York Oxford Oxford University Press 1997 Oxford University Press Oxford New York Athens Auckland Bangkok Bogota Bombay Buenos Aires Calcutta Cape Town Dar es Salaam Delhi Florence Hong Kong Istanbul Karachi Kuala Lumpur Madras Madrid Melbourne Mexico City Nairobi Paris Singapore Taipei Tokyo Toronto and associated companies in Berlin Ibadan Copyright © 1997 by Oxford University Press, Inc. Published by Oxford University Press, Inc. 198 Madison Avenue, New York, New York 10016 Oxford is a registered trademark of Oxford University Press All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or means, electronic, mechanical, photocopying, recording, or otherwise, without prior permission of Oxford University Press. Library of Congress Cataloging-in-Publication Data Biological NMR spectroscopy / edited by John L. Markley, Stanley J. Opella. p. cm. Includes bibliographical references and index. ISBN 0-19-509468-9 I. Nuclear magnetic resonance spectroscopy. 2. Biomolecules—Analysis. I. Markley, John L. II. Opella, Stanley J. QP519.9.N83B56 1997 574.19785—dc20 96-12586 9 8 7 6 5 4 3 2 1 Printed in the United States of America on acid-free paper Table of Contents Introduction ix Acknowledgments .xi Foreword: R.R. Ernst .xiii The Founders Medal xv Section 1: History of Biological NMR Spectroscopy 1 Chapter 1: Simple Insights from the Beginnings of Magnetic Resonance in Molecular Biology 3 0. Jardetzky Chapter 2: Choice of Problems in the Early Days of Biological NMR Spectroscopy 16 M. Cohn Chapter 3: Early Days of Biochemical NMR. 20 R.G. Shulman Chapter 4: William D. Phillips Memorial Lecture 23 J.J.H. Acker man Section 2: Protein Structural Studies. .27 Chapter 5: Flexibility and Function of the Excherichia coli trp Represser 29 M.R. Gryk and O, Jardetzky Chapter 6: Heteronuclear Strategies for the Assignment of Larger protein/DNA complexes: Application to the 37 kDa trp Represser-Operator Complex 50 M.J. Revington, W. Lee, and C.H. Arrowsmith Chapter 7: Design of Novel Hemoglobins 67 C, HoandH.-W. Kim Chapter 8: The Role of NMR Spcclroscopy in Understanding How Proteins Fold... .82 C.M. Dob son vi Biological NMR Spectroscopy Chapter 9: NMR Approaches To Understanding Protein Specificity 94 G.C.K. Roberts, L.-Y. Lian, I.L. Barsukov, S. Modi, and W. U. Primrose Chapter 10: NMR and Mutagenesis Investigations of a Model Cis:Trans Peptide Isomerization Reaction: Xaa116-Pro117 of Staphylococcal Nuclease and its Role in Protein Stability and Folding 113 A.P. Hinck, W.F. Walkenhorsl, DM. Truckses, and J.L. Markley Chapter 11: A Solid-State NMR Approach to Structure Determination of Membrane Associated Peptides and Proteins .139 S.J. Opella, L.E. Chirlian, and R. Bechinger Chapter 12: NMR Approaches to the Heat-, Cold-, and Pressure Induced Unfolding of Proteins 157 K. Akasaka, T. Yamaguchi, H. Yamada, Y. Kamatari, and T. Konno Chapter 13: Multidimensional NMR Investigation of the Neurotoxic Peptide Mastoparan in the Absence and Presence of Calmodulin 169 F. Mari, X. Xie, J.H. Simpson, and D.J. Nelson Chapter 14: NMR of Larger Proteins: An Approach to the Structural Analyses of Antibody 183 Y. Arata Chapter 15: Selective Chemical Deuteration of Aromatic Amino Acids: A Retrospective 205 K.S. Matthews and R. Matthews Chapter 16: The Interaction of Antigens and Superantigens with the Human Class II Major Histocompatibility Complex Molecule HLA-DR1 216 T. Jardetzky Chapter 17: Chorismate Mutase, Essentially a Template Enzyme 235 W.N. Lipscomb, Y.M. Chook, and H. Ke Contents vii Section 3: Nucleic Acids 245 Chapter 18: Computing the Structure of Large Complexes; Modeling the 16S RibosomalRNA 247 R. Chen, D. Fink, and R.B. Altrnan Chapter 19: Design and Characterization of New Sequence Specific DNA Ligands 266 D. Wemmer Chapter 20: Determination by 1H NMR of a Slow Conformational Transition and Hydration Change in the Consensus TATAAT PribnowBox .277 C. Milhe, A. Lane, andJ.-F. Lefevre Section 4: In vivo Spectroscopy 301 Chapter 21: Clinicians Need Localized Proton MRS of the Brain in the Management of HIV-Related Encephalopathies 303 P.J. Cozzone, J. Vion-Dury, S, Confort-Gouny, F. Nicoli, A. -M. Salvan, and S. Lamoureux Chapter 22: Nuclear Magnetic Resonance Spectroscopy Studies of Cancer Cell Metabolism 317 O. Kaplan and J.S, Cohen Chapter 23: Ex Vivo Multinuclear NMR Spectroscopy of Perfused, Respiring Rat Brain Slices: Model Studies of Hypoxia, Ischemia.and Excitotoxicity" 340 L. Litt, M.T. Espanol, Y. Xu, Y. Cohen, L.-H. Chang, P.R. Weinstein, P.H. Chan, and T.L. James Index 359 This page intentionally left blank Introduction The founders of the field of biological nuclear magnetic resonance (NMR) spectroscopy had a clear vision of its future potential. The 65th birthday of one of these pioneers, Professor Oleg Jardetzky, provided an occasion for bringing together the small group of scientists whose research defined the field at its inception, Jardetzky and his mentors, Linus Pauling and William N. Lipscomb, and Mildred Cohn and Robert G. Shulman. Another key figure in the early days of the field, William D. Phillips who was expected to participate in the Symposium and this book died shortly before the Symposium held at Stanford University in March, 1994. The historical section of this volume (Section I) conveys the excitement of the beginnings of biological NMR spectroscopy, when every experiment was new and potentially important. Section II, which constitutes the bulk of this Volume, provides a contemporary overview of the legacy of these early experiments. It is nothing short of astonishing to be able to say that biological NMR spectroscopy has fulfilled the dreams of its founders. Its potential as a method for determining structures and describing the dynamics of proteins and nucleic acids in aqueous solution has been realized in recent years, and the approachhas become an essential part of structural biology. These capabilities, which are practically routine now, reflect the rapid pace of technological invention and the scientific context of the period, 1957 to the present. Invention refers to the continuous stream of advances in instrumentation and magnets, computers and software, pulse sequences, and experimental design for NMR studies of proteins and other biopolymers. Context refers to the advances in biology. Even the most casual reader of newspaper headlines is aware that we live in the age of molecular biology, since it is now recognized that all aspects of life reflect the linear sequence of bases in DNA that store biological information and, in turn, specify the amino acid sequences of the proteins that express biological functions. However, we also realize that biology is fundamentally both three- dimensional and dynamic. And here is where structural biology will come to play the dominant role in describing the basic principles of biology and furthering biomedical andbiotechnological applications. The limits of what can be obtained from sequences alone are already at hand. The limits of what can be obtained from structural biology, in general, and biological NMR spectroscopy, in particular, have not even been approached. The frontiers of protein complexes, membrane proteins, and carbohydrates, as well as more detailed descriptions of the interplay between structure and dynamics in protein function beckon. Oleg Jardetzky is one of the pillars of biological NMR spectroscopy. He identified at its earliest stages the importance of resolving and assigning resonances from individual sites in a protein. This enabled the unique ability of

See more

The list of books you might like

Most books are stored in the elastic cloud where traffic is expensive. For this reason, we have a limit on daily download.