Biochromatography-Theory and Practice

649 Pages·2002·8.661 MB·\649

by  M. A. Vijayalakshmi (Editor)

Biochromatography

Biochromatography
Theory and practice
Edited by
M.A.Vijayalakshmi

London and New York
First published 2002
by Taylor & Francis
11 New Fetter Lane, London EC4P 4EE
Simultaneously published in the USA and Canada
by Taylor & Francis Inc,
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© 2002 Taylor and Francis
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Every effort has been made to ensure that the advice and information
in this book is true and accurate at the time of going to press. However,
neither the publisher nor the authors can accept any legal responsibility
or liability for any errors or omissions that may be made. In the case of
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ISBN 0-203-30282-6 Master e-book ISBN
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I would like to thank, from the depth of my heart, Prof J.Porath who initiated me into this
field, guided and encouraged me throughout, and my colleague and friend Prof
E.Sulkowski for his sustained interest and advice. I dedicate this book to them.

Contents

List of contributors ix
Acknowledgements xii
Preface xiv
Foreword xvi

1 Upstream and downstream steps in biochromatography 1

MOOKAMBESWARAN A.VIJAYALAKSHMI
2 Gel filtration 11

KJELL-OVE ERIKSSON
3 Ion exchange interaction biochromatography 29

E.BOSCHETTI AND P.GIROT
4 Hydrophobic (interaction) chromatography of proteins 55

HERBERT P.JENNISSEN
5 Conformational behaviour of polypeptides and proteins in reversed phase 85
and lipophilic environments
MILTON T.W.HEARN
6 Affinity chromatography 172

JAROSLAVA TURKOVA
7 Dye ligand affinity chromatography 269

ROBERT K.SCOPES
8 Immobilized synthetic dyes in affinity chromatography 281

NIKOS E.LABROU AND YANNIS D.CLONIS
9 Immobilized histidine ligand affinity chromatography 301

OLIVIER PITIOT AND MOOKAMBESWARAN A.VIJAYALAKSHMI
10 Immobilized metal-ion affinity chromatography: from phenomenological 326
hallmarks to structure-based molecular insights
NADIR T.MRABET AND MOOKAMBESWARAN A.VIJAYALAKSHMI
11 Thiophilic interaction chromatography 353

SVEN OSCARSSON AND FRANCISCO BATISTA VIERA
12 Miscellaneous methods in affinity chromatography Part 1: Boronic acids 367
as selective ligands for affinity chromatography
XIAO-CHUAN LIU AND WILLIAM H.SCOUTEN
Part 2: Shielded affinity chromatography in packed bed and expanded bed 379
mode
IGOR YU.GALAEV AND BO MATTIASSON
13 Glycobiology and biochromatography 392

HENRI DEBRAY, GÉRARD STRECKER AND JEAN MONTREUIL
14 Capillary electrokinetic chromatography 453

GARGI CHOUDHARY AND CSABA HORVÁTH
15 Imprinted polymers as tailor-made stationary phases for affinity 499
separation
KARSTEN HAUPT, PETER A.G.CORMACK AND KLAUS MOSBACH
16 Computer-aided simulation of biochromatography 514

NICOLAS VOUTE
17 Industrial biochromatography: engineering aspects 554

F.A.P.GARCIA, D.M.F.PRAZERES AND J.M.S.CABRAL
18 Validation aspects in biochromatography 572

CHARLES LUTSCH
19 Biochromatography and biomedical applications 588
C.LEGALLAIS, MOOKAMBESWARAN A.VIJAYALAKSHMI AND
PH.MORINIÉRE

Index 614
Contributors
Francisco Batista Viera Catedra de Bioquimica, Facultad de Zuimica, Gral, Flores
2124, Casilla de Correo 1157, Montevideo, Uruguay.
E.Boschetti Biosepra S.A, 35 Avenue Jean Jaurés, 92390 Villeneuve la Garenne, France.
J.M.S.Cabral Instituto Superior Tecnico, Departamento de Engenharia Quimica, Secçao
de Biotecnologia, Avenue Rouisco Pais, 1096 Lisboa Codex, Portugal.
Gargi Choudhary Berlex Biosciences, 15049 San Pablo Avenue, Richmond, CA
94804–0099, USA.
Yannis D.Clonis Enzyme Technology Laboratory, Department of Agricultural
Biotechnology, The Agricultural University of Athens, Iera Odos 75, GR 11855
Athens, Greece.
Peter A.G.Cormack Department of Pure and Applied Chemistry, University of
Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow, Scotland.
Henri Debray Laboratoire de Chimie Biologique, Universié des Sciences et
Technologies de Lille 1, UMR CNRS 8576, 59655 Villeneuve d’Ascq Cedex, France.
Kjell-Ove Eriksson Amersham Biosciences, Björkgatan 30, 751 84 Uppsala, Sweden.
Igor Yu.Galaev Department of Biotechnology, Center for Chemistry and Chemical
Engineering, Lund University, P.O. Box 124, Lund S-221 00, Sweden.
F.A.P.Garcia Departmento de Engenharia Quimica, Universidade de Coimbra, 3000
Coimbra, Portugal.
P.Girot Biosepra S.A., 35 avenue Jean Jaurés, 92390 Villeneuve la Garenne, France.
Karsten Haupt INTS U76 INSERM, 6 rue Alexandre Cabarel, 75739 Paris Cedex 15,
France.
Milton T.W.Hearn Center for Bioprocess Technology, Department of Biochemistry and
Molecular Biology, Monash University, Wellington Road, Clayton, 3168 Victoria,
Australia.
Csaba Horváth Department of Chemical Engineering, Yale University, Mason Lab.,
P.O. 208286, 9 Hill House Avenue, Newhaven CT 06520, USA.
Herbert P.Jennissen Institut für Physiologische Chemie, Universität GHS Essen,
Hufeland str. 55, D 45122 Essen, Germany.
Nikos E.Labrou Enzyme Technology Laboratory, Department of Agricultural
Biotechnology, The Agricultural University of Athens, Iera Odos 75, GR 11855
Athens, Greece.
C.Legallais Université de Technologie de Compiègne, Département de Génie
Biologique, UMR CNRS 6600, BP 20.529, 60205 Compiègne Cedex, France.
Xiao-Chuan Liu Department of Chemistry, Indiana State University, Terre Haute, IN
47809.
Charles Lutsch Director of the Department of Protein Purification, Pasteur Mérieux