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Biochemistry Biochimiedes and Cell Biology et biologie cellulaire Contents Sommaire Volume 83, 2005 Volume 83, 2005 February / Février MINIREVIEW / MINISYNTHESE Katherine L. Dunn, Paula S. Espino, Bojan Drobic, Shihua He, and James R. Davie The Ras-MAPK signal transduction pathway, cancer and chromatin remodeling ARTICLES / ARTICLES Maristela Miyamoto, Benedicto C. Vidal, and Maria Luiza S. Mello Chromatin supraorganization, DNA fragmentation, and cell death in snake erythrocytes A.K. Dudani, M. Ben-Tchavtchavadze, S. Porter, and E. Tackaberry Angiostatin and plasminogen share binding to endothelial cell surface actin Kohei Miura, Shashi Uniyal, Mircea Leabu, Tamas Oravecz, Subrata Chakrabarti, Vincent L. Morris, and Bosco M.C. Chan Chemokine receptor CXCR4-}1 integrin axis mediates tumorigenesis of osteosarcoma HOS cells Mamiko Shimizu, Jan K. Losos, and Ann M. Verrinder Gibbins Analysis of an approach to oviduct-specific expression of modified chicken lysozyme genes Malcolm B. Perry, Leann L. MacLean, and Evgeny Vinogradov Structural characterization of the antigenic capsular polysaccharide and lipopolysaccharide O-chain produced by Actinobacillus pleuropneumoniae serotype 15 Anne Belzil et Carmen Parent Méthodes de qualification des immobilisations chimiques d'une enzyme sur un support solide T.B. Ng, W. Gao, L. Li, S.M. Niu, L. Zhao, J. Liu, L.S. Shi, M. Fu, and F. Liu Rose (Rosa rugosa)-flower extract increases the activities of antioxidant enzymes and their gene expression and reduces lipid peroxidation Sumit J. Shah and Paul W. Sylvester Tocotrienol-induced cytotoxicity is unrelated to mitochondrial stress apoptotic signaling in neoplastic mammary epithelial cells . Tasevski, J.M. Sorbetti, S.S. Chiu, N.G. Shrive, and D.A. Hart Influence of mechanical and biological signals on gene expression in human MG-63 cells: evidence for a complex interplay between hydrostatic compression and vitamin D3 or TGF-BI on MMP-1I and MMP-3 mRNA levels Instructions to Authors Recommandations aux auteurs April / Avril ARTICLES / ARTICLES Hong-Min Tang and Hong Yu Intermediate studies on refolding of arginine kinase denatured by guanidine hydrochloride Jacqueline N. Watson, Tara L. Knoll, Johnny H. Chen, Doug T.H. Chou, Thor J. Borgferd, and Andrew J. Bennet Use of conformationally restricted pyridinium @-D-N-acetylneuraminides to probe specificity in bacterial and viral sialidases J.J. Provost, S.M. Olmschenk, A.L. Metcalf, N. Korpi, H. Thronson, M. Liu, and M.A. Wallert Phospholipase C-, mediates ,-adrenergic receptor-stimulated activation of the sodium—hydrogen exchanger in Chinese hamster lung fibroblasts (CCL39) Florian Graichen, Kristian R. Giles, Andrew Abell, and Ashley Garrill Removal! of the nitro and pheny! groups from NPPB decreases its inhibitory effect on cytoplasmic streaming in the alga Nitella hookeri Ji-Cheng Pan, Jin-Song Wang, Yuan Cheng, Zhenhang Yu, Xue-Ming Rao, and Hai-Meng Zhou = The role of detergent in refolding of GdnHCl-denatured arginine kinase from shrimp Fenneropenaeus Chinensis: the solubilization of aggregate and refolding in detergent solutions Francois Vigneault and Régen Drouin Optimal! conditions and specific characteristics of Vent exo— DNA polymerase in ligation-mediated polymerase chain reaction protocols © 2005 NRC Canada C-2 Biochem. Cell Biol. Vol. 83, 2005 Lello Zolla and Anna Maria Timperio Involvement of active oxygen species in protein and oligonucleotide degradation induced by nitrofurans Anna Maria Bassi, Sabina Ledda, Maria Clara De Pascale, Susanna Penco, Simona Rossi, Patrizio Odetti, and Damiano Cottalasso Antioxidant status in J774A.1 macrophage cell line during chronic exposure to glycated serum Chenchen Lin, Meiyao Lin, and Hungwen Chen Biochemical characterization of the human placental transcription factor GCMa/! Laura Bandura, Justyna Drukala, Agnieszka Wolnicka-Glubisz, Mikael Bjérnstedt, and Wlodzimierz Korohoda Differential effects of selenite and selenate on human melanocytes, keratinocytes, and melanoma cells Patrick H.K. Ngai and T.B. Ng Phaseococcin, an antifungal protein with antiproliferative and anti-HIV-1 reverse transcriptase activities from small scarlet runner beans Birgit Kindermann, Frank Déring, Jan Budczies, and Hannelore Daniel Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) Jan K. Losos, David H. Evans, and Ann M. Verrinder Gibbins Targeted modification of the complete chicken lysozyme gene by poxvirus-mediated recombination Kenji Takase, Kiyoshi Hagiwara, Haruko Onodera, Yaeko Nishizawa, Masashi Ugaki, Toshihiro Omura, Shinichi Numata, Katsuki Akutsu, Haruto Kumura, and Kei-ichi Shimazaki Constitutive expression of human lactoferrin and its N-lobe in rice plants to confer disease resistance June / Juin Epigenetics in Chromatin / Epigenése chromatinienne MINIREVIEW / MINISYNTHESE Jordanka Zlatanova MeCP2: the chromatin connection and beyond REVIEWS / SYNTHESES Shiao Li Ocei, Claudia Keil, and Mathias Ziegler Poly(ADP-ribosylation) and genomic stability Paul O. Hassa and Michael O. Hottiger An epigenetic code for DNA damage repair pathways? Iidik6 Gyéry and Janos Minarovits Epigenetic regulation of lymphoid specific gene sets Michéle J. Hoffmann and Wolfgang A. Schulz Causes and consequences of DNA hypomethylation in human cancer Filippo M. Cernilogar and Valerio Orlando Epigenome programming by Polycomb and Trithorax proteins Raffaella Santoro and Filomena De Lucia Many players, one goal: how chromatin states are inherited during cell division Loredana Verdone, Micaela Caserta, and Ernesto Di Mauro Role of histone acetylation in the control of gene expression Maria Malanga and Felix R. Althaus The role of poly(ADP-ribose) in the DNA damage signaling network Melita Vidakovic, Goran Poznanovic, and Juergen Bode DNA break repair: refined rules of an already complicated game Esteban Ballestar and Manel Esteller Methyl-CpG-binding proteins in cancer: blaming the DNA methylation messenger Irina Stancheva Caught in conspiracy: cooperation between DNA methylation and histone H3K9 methylation in the establishment and maintenance of heterochromatin Maria Rosaria Faraone-Mennella Chromatin architecture and functions: the role(s) of poly(ADP-RIBOSE) polymerase and poly(ADPribosyl)ation of nuclear proteins August / Aodt 26th International West Coast Chromatin and Chromosomes Conference / 26° conférence internationale de la céte Quest sur la chromatine et les chromosomes MINIREVIEWS / MINISYNTHESES Cotteka N. Johnson, Nicholas L. Adkins, and Philippe Georgel Chromatin remodeling complexes: ATP-dependent machines in action Wei Xu_ Nuclear receptor coactivators: the key to unlock chromatin Hidenobu Soejima, Wei Zhao, and Tsunehiro Mukai Epigenetic silencing of the MGMT gene in cancer Zhao-xia Chen and Arthur D. Riggs Maintenance and regulation of DNA methylation patterns in mammals Martin Toussaint, Genevieve Levasseur, Maxime Tremblay, Michel Paquette, and Antonio Conconi Psoralen photocrosslinking, a tool to study the chromatin structure of RNA polymerase I - transcribed ribosomal genes © 2005 NRC Canada Contents / Sommaire Adam Wood, Jessica Schneider, and Ali Shilatifard Cross-talking histones: implications for the regulation of gene expression and DNA repair Michael S. Cosgrove and Cynthia Wolberger How does the histone code work? Harald Biessmann, Sudha Prasad, Marika F. Walter, and James M. Mason’ Euchromatic and heterochromatic domains at Drosophila telomeres Zachary F. Burton, Michael Feig, Xue Q. Gong, Chunfen Zhang, Yuri A. Nedialkov, and Yalin Xiong NTP-driven translocation and regulation of downstream template opening by multi-subunit RNA polymerases Benoit Coulombe and Marie-France Langelier Functional dissection of the catalytic mechanism of mammalian RNA polymerase II Andra Li, José M. Eirin-Lépez, and Juan Ausi6 H2AX: tailoring histone H2A for chromatin-dependent genomic integrity REVIEWS / SYNTHESES Hui Zhao and Ann Dean Organizing the genome: enhancers and insulators Benjamin A.T. Rodriguez and Tim H.-M. Huang _ Tilling the chromatin landscape: emerging methods for the discovery and profiling of protein-DNA interactions Gareth N. Corry and D. Alan Underhill Subnuclear compartmentalization of sequence-specific transcription factors and regulation of eukaryotic gene expression Kathrin Muegge Lsh, a guardian of heterochromatin at repeat elements ARTICLE / ARTICLE Philippe T. Georgel Chromatin potentiation of the hsp70 promoter is linked to GAGA-factor recruitment ABSTRACTS / RESUMES Zhao-xia Chen and Arthur D. Riggs Self-association of human de novo DNA methyltransferases, DNMT3A and DNMT3B Giscard H. Yanez, Sheik J. Khan, Alexandra M. Locovei, Ilene M. Pedroso, and Terace M. Fletcher The role of DNA structure in telomere function A. Weyrich, M. Dong, Y. Ge, G. Xu, and W. Hennig Expression of human DNA-methylase Dnmt3b in the male germ line of Drosophila W. Hennig, R. Feng, A. Weyrich, X. Tang, H. Li, Y. Lu, M. Dong, and Y. Chen’ A maternal-effect dE2// mutation of Drosophila melanogaster H. Biessmann, M.F. Walter, and J.M. Mason Heterochromatin at Drosophila telomeres Rocio Montes de Oca and Katherine L. Wilson Selective binding of barrier-to-autointegration factor (BAF) to core histone H3 and linker histone H1.1 A. Prado, I. Ramos, A. Muga, R. Finn, and J. Ausié6 The biological activity of nucleoplasmin: how many partners? Andra Li and Juan Ausi6é Characterization of human testis- and sperm-specific H2B (hTSH2B) nucleosomes Sara S. Dirscherl, Jonathan J. Henry, and Jocelyn E. Krebs Neural and eye-specific defects associated with loss of the imitation switch (ISWI) chromatin remodeler in Xenopus laevis John D. Moore and Jocelyn E. Krebs Histone H2A modifications distinguish pathways of double-strand break repair Hui-Ching Kuo, John D. Moore, and Jocelyn E. Krebs Spt! and histone H2A cooperate to regulate copper homoeostasis M. Paquette and A. Conconi UV-induced DNA lesions trigger nucleosomes deposition onto active rDNA in the absence of the damage recognition protein Rad14 M. Toussaint, M. Tremblay, M. Paquette, R.J. Wellinger, and A. Conconi DNA repair of UV damage in yeast: effect of SIR2 deletion R. Bash, H. Wang, G.L Hager, J. Yodh, D. Lohr, and S. Lindsay Chromatin studies with AFM recognition imaging N.P. Thorogood and C.J. Brown Determining the connection between XIST/Xist expression and features of X-inactivation in human/mouse somatic cell hybrids D.A. Underhill, V.L. Biron, N. Hu, and M.J. Hendzel Dynamics of histone lysine methylation in mouse development G.N. Corry and D.A. Underhill Examining the relationship between homeodomain DNA binding, chromatin interaction, and subnuclear localization N.L. Adkins and P.T. Georgel Potential links between cel! differentiation in salivary glands and chromatin remodeling Diane P. Genereux, Brooks E. Miner, Carl T. Bergstrom, and Charles D. Laird Cellular population epigenetics of methylation © 2005 NRC Canada C-4 Biochem. Cell Biol. Vol. 83, 2005 M. Anderson, T. Ngo, J. Huh, A. Ing, G. Hernandez, J. Pang, J. Perkins, and R. Dutnall A method for the production of soluble histones in bacteria Katherine L. Dunn and James R. Davie Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of H3 on serine 10 and 28 Jianxun Han, Michael Hendzel, and Joan Turner Objective analysis and comparison of y-H2AX foci Feixiong Zhang, Yubo Han, Jiangmei Yin, Fang Fang, and Ning Li The transcription localization of RNA polymerase III is in or at the edge of the nucleolus Amy Dhirapong, Janis Alien, Johnny Chavez, Leonel Santibaiiez-Vargas, Susan L. Forsburg, and Sally G. Pasion Analysis of fission yeast cdc24 replication mutant genetic interactions with checkpoint kinases and testing of mutator phenotype Charles D. Laird, Brooks E. Miner, Alice F. Burden, R. Scott Hansen, and Reinhard J. Stéger Epigenetic methylation of DNA at the fragile X locus, FMR/: insights from analyzing both strands of individual DNA molecules A. Weyrich, M. Dong, Y. Ge, G. Xu, and W. Hennig Expression of mammalian DNA-methylase Dnmt3b in the male germ line of Drosophila October / Octobre REVIEW / SYNTHESE Claudio A. Hetz, Vicente Torres, and Andrew F.G. Quest Beyond apoptosis: nonapoptotic cell death in physiology and disease ARTICLES / ARTICLES Eleonora Altman, Blair A. Harrison, Tomoko Hirama, Vandana Chandan, Rebecca To, and Roger MacKenzie Characterization of murine monoclonal antibodies against Helicobacter pylori lipopolysaccharide specific for Le‘ and Le* blood group determinants Ramesh Natarajan, Drew G. Jones, Bernard J. Fisher, Timothy J. Wallace, Shobha Ghosh, Alpha A. Fowler, II Hypoxia inducible factor-1: regulation by nitric oxide in posthypoxic microvascular endothelium Jennifer Y. Kong and Simon W. Rabkin The association between RhoB and caspase-2: changes with lovastatin-induced apoptosis Mohamed Abu-Farha, Jacques Niles, and William G. Willmore —Erythroid-specific 5-aminolevulinate synthase protein is stabilized by low oxygen and proteasomal inhibition Gregory D. Fairn and Christopher R. McMaster The roles of the human lipid-binding proteins ORP9S and ORPIOS in vesicular transport Michael D. Matchett, Shawna L. MacKinnon, Marva I. Sweeney, Katherine T. Gottschall-Pass, and Robert A.R. Hurta Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells I.E. Mazzoni, H.C. Ledebur, Jr., E. Paramithiotis, and N. Cashman Lymphoid signal transduction mechanisms linked to cellular prion protein Yurong Liang, Wei Li, Qing Ma, and Yuying Zhang = Functiona! analysis of tunicamycin-inducible gene A polypeptide from Aspergillus niger Veruska B.M. Alencar, Gerly A.C. Brito, Nylane M.N. Alencar, Ana M.S. Assreuy, Vicente P.T. Pinto, Edson H. Teixeira, Emmanuel P. Souza, Henri Debray, Ronaldo A. Ribeiro, and Benildo S. Cavada Helianthus tuberosus agglutinin directly induces neutrophil migration, which can be modulated/inhibited by resident mast cells Kirsten I. Larsen, Marina Falany, Wei Wang, and John P. Williams Glucose is a key metabolic regulator of osteoclasts: glucose stimulated increases in ATP/ADP ratio and calmodulin kinase II activity NOTES / NOTES Ashley C. Wozniak and Judy E. Anderson Single-fiber isolation and maintenance of satellite cell quiescence Reijo Kakela, Kimmo Tanhuanpiia, Saara Laitinen, Pentti Somerharju, and Vesa M. Olkkonen Overexpression of OSBP-related protein 2 (ORP2) in CHO cells induces alterations of phospholipid species composition Con K. Vitou, Xiaoming Li, and Barid Mukherjee — Inhibition of colony formation by mouse mammary cancer cells co-cultured with early embryonic cells © 2005 NRC Canada Contents / Sommaire December / Decembre CSBMCEB - Celiular Dynamics / SCBBMC — Dynamique cellulaire MINIREVIEWS / MINISYNTHESES Christopher V. Nicchitta, Rachel S. Lerner, Samuel B. Stephens, Rebecca D. Dodd, and Brook Pyhtila Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model David Bouck and Kerry Bloom = The role of centromere-binding factor 3 (CBF3) in spindle stability, cytokinesis, and kinetochore attachment Rongmin Zhao and Walid A. Houry Hsp90: a chaperone for protein folding and gene regulation REVIEWS / SYNTHESES Hassina Darenfed and Craig A. Mandato Wound-induced contractile ring: a model for cytokinesis R. Scott Williams, Nina Bernstein, Megan S. Lee, Melissa L. Rakovszky, Diana Cui, Ruth Green, Michael Weinfeld, and J.N. Mark Glover Structural basis for phosphorylation-dependent signaling in the DNA-damage response Andrew J. Crofts, Haruhiko Washida, Thomas W. Okita, Mio Satoh, Masahiro Ogawa, Toshihiro Kumamaru, and Hikaru Satoh The role of mRNA and protein sorting in seed storage protein synthesis, transport, and deposition Eric D. Brown Conserved P-loop GTPases of unknown function in bacteria: an emerging and vital ensemble in bacterial physiology R. Chris Bleackley A molecular view of cytotoxic T lymphocyte induced killing Paula Estrada de Martin, Peter Novick, and Susan Ferro-Novick The organization, structure, and inheritance of the ER in higher and lower eukaryotes ABSTRACTS / RESUMES D. Bouck and K. Bloom Kinetochore proteins in cytokinesis y.Y.C. Tam, A. Fagarasanu, M. Fagarasanu, and R.A. Rachubinski Pex3p initiates the formation of a preperoxisomal compartment from a subdomain of the ER in Saccharomyces cerevisiae M. Fagarasanu, A. Fagarasanu, Y.Y.C. Tam, J.D. Aitchison, and R.A. Rachubinski Inp!p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae M.J. Young and D.A. Court The yeast mitochondrial DNA polymerase harbours a carboxy! terminal extension essential for respiratory competence D.W. Van de Vosse, R.J. Scott, C.P. Lusk, D.J. Dilworth, J.D. Aitchison, and R.W. Wozniak — Functional links between the nuclear pore complex protein Nup60p and DNA replication R.J. Scott, C.P. Lusk, and R.W. Wozniak The nuclear transport machinery controls the localization of Mad|p and modulates its function in the spindle assembly checkpoint N. Quenneville, C. Chao, and E. Conibear Role of Vps54 in the GARP complex: implications for compartment recognition by a tethering complex N. Park and D.G. Muench = Characterization of exon—-exon junction components in plant cells N. Najand and A.J. Simmonds Deriving a consensus sequence necessary for mRNA localization D. Mason, G.V. Mallo, M.R. Terebiznik, B. Payrastre, B.B. Finlay, J.H. Brumell, and S. Grinstein Alteration of epithelial structure and function associated with PtdIns(4,5)P, degradation by a bacterial phosphatase L.M. Luft, E.K.L. Chan, M.J. Fritzler Characterization of a novel cytoplasmic organelle, GW bodies, as a site of mRNA localization in normal and malignant breast cell lines and tissues R.S. Lerner, M.C. Reedy, and C.V. Nicchitta The role of protein synthesis in the regulation of mRNA partitioning to the endoplasmic reticulum J. Haskins and A.J. Simmonds Screening for cis- and trans-acting factors involved in mRNA localization in later stages of Drosophila development L.A. Hawryluk-Gara, E.K. Shibuya, and R.W. Wozniak Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex H. Goldie and K. Klemmer Changes in structure and function of phosphoenolpyruvate carboxykinase from Escherichia coli when activation by Ca~* is abolished by partial digestion with trypsin A.T. McGuire, H. Dehghani, R.A.B. Keates, $.C. Chafe, and D. Mangroo Cex!p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery that may play a role in unloading of (RNA from the tRNA nuclear export receptor complex at the NPC © 2005 NRC Canada C-6 Biochem. Cell Biol. Voi. 83, 2005 J.B. Dacks, M.C. Field, R.P. Hirt, L. Gedamu, T.M. Embley, and W.F. Doolittle Reconstructing the secretory system of the last common eukaryotic ancestor ; S. Cohen, A. Behzad, J. Carroil, and N. Panté Nuclear import of parvovirus mice minute virus R.W. Ching, G. Dellaire, C.H. Eskiw, and D.P. Bazett-Jones Nuclear fission and fusion: PML nuclear body dynamics in mammalian cells F.J. Vizeacoumar, W.N. Vreden, J.D. Aitchison, and R.A. Rachubinski The Pex|9p-binding regions of peroxins Pex30p and Pex32p are required for their peroxisomal localization but are separate from their peroxisomal targeting signals J.D Chapman, M.H. Simonian, E. Betgovargez, and J.S. Hobbs Applying a divide and conquer strategy to biomarker discovery C.M. Dobson and R.A. Rachubinski What proteins impact Sup35 foci and filament formation? A. Fagarasanu, M. Fagarasanu, G. Eitzen, J.D. Aitchison, and R.A. Rachubinski Inp2p is a peroxisomal protein required for the Myo2p-driven transport of peroxisomes during cell division in Saccharomyces cerevisiae J.S. Williams and M.C. Schultz Harnessing a global regulator of chromatin structure for gene-specific control of transcription B.R. Strub, M. Steiner-Mosonyi, and D. Mangroo Utp8p is required for the transport of tRNAs from the nucleolus to nuclear export receptors in Saccharomyces cerevisiae V.M.I. Provencher and L.M. Schang Protein—protein phosphorylation is required for recruitment of ICP4 to nuclear replication compartment D. Pinchev, J. Taylor, D. Vasquez, J.L. Howell, and R. Truant Ataxin-3, a nucleocytoplasmic shuttling protease: implications for Machado—Joseph disease T. Makhnevych, J.D. Aitchison, and R.W. Wozniak Kap!2!p regulations the SUMOylation of the septin Cdc3p C.P. Lusk, D. Waller, T. Makhnevych, D. Thomas, M. Whiteway, and R.W. Wozniak NupS53p is a target of two mitotic kinases, CdkIp and Hrr25p L. Li and J.R. Davie The role of Sp! and Sp3 in the regulation of estrogen-responsive genes in breast cancer cell lines C. Gregg, T. Guo, T. Boukh-Viner, A. Alexandrian, F. Banu, A. Cerracchio, S. Haile, L. Makoroka, S. Milijevic, C. Sison, J. Soiomon, V. Wong, J.-M. Nicaud, R.A. Rachubinski, A.M. English, and V.I. Titorenko Molecular mechanism for the coordination of organelle growth and division J. Goetz, H. Genty, A. Raz, R. Sauvé, and L.R. Nabi Regulation of smooth ER — mitochondria association in normal and transformed cells by cytosolic free calcium E. Cordat, S. Kittanakom, P.-T. Yenchitsomanus, J. Li, K. Du, G.L. Lukacs, and R.A.F. Reithmeier Trafficking defects of dominant and recessive distal renal tubular acidosis mutants of the kidney anion exchanger | S.D.X. Chuong, N.-I. Park, M.C. Freeman, R.T. Mullen, and D.G. Muench Protein trafficking to the peroxisome: a role for mRNA~cytoskeleton interactions? F. Brandizzi, S. Hanton, L. Renna, L. Bortolotti, and G. Stefano Dynamics of the endoplasmic reticulum and Golgi apparatus in live plant cells D.C. Bay, J.D. O'Neil, and D.A. Court Mitochondrial porins on a cholesterol-free diet: do sterols influence tertiary interactions in detergent-solubilized porins? A.M. Anderson, R.J. Scott, D.J. Dilworth, J.D. Aitchison, and R.W. Wozniak Identification of a novel microtubule related function for the B-karyopherin Kap123p R. Zhao, M. Davey, Y.-C. Hsu, P. Kaplanek, A. Tong, A.B. Parsons, N. Krogan, G. Cagney, D. Mai, J. Greenblatt, C. Boone, A. Emili, and W.A. Houry Navigating the chaperone network: an integrative map of physical, genetic, and chemical—genetic interactions mediated by the yeast Hsp90 chaperone system R.H. Singer, Y. Shav-Tal, X. Darzacq, S. Huettelmaier, A. Rodriguez, W. Gu, and S. Shenoy Observing RNA synthesis, movement, and localization in living cells T.W. Okita ER retention or export: the role of RNA localization Christopher Nicchitta Localized mRNA translation at the endoplasmic reticulum: the unfolded protein response reveals new mechanisms for the compartmentalization of protein synthesis Peter S. McPherson Molecular mechanisms in clathrin-mediated endocytosis revealed through quantitative subcellular proteomics Trey Ideker Complex biological queries on the protein interaction network Robert H. Singer, Yaron Shavy-Tal, Xavier Darzacq, Stefan Huettelmaier, Alex Rodriguez, Wei Gu, and Shailesh Shenoy Observing RNA synthesis, movement, and localization in living cells F. Neumann, M. Gartenberg, A. Taddei, F. Hediger, K. Bystricky, T. Laroche, G. van Houwe, M. Blaszezyk, and S.M. Gasser Chromosome looping, telomere anchoring, and the functional impact of yeast chromatin dynamics © 2005 NRC Canada Contents / Sommaire Cayetana Schluter, Jochen Brumm, Jenny Bryan, and Elizabeth Conibear Global discovery of Golgi-endosomal transport complexes in yeast Jon B. Carmichael, Cezar Stoica, Henry Parker, !. Michael McCaffery, and Tom C. Hobman Dynamic localization of RNA interference effector proteins in Schizosaccharomyces pombe Craig A. Mandato Analysis of cytokinesis in cells that do not divide Jennifer J. Smith and John D. Aitchison Systems approach to deciphering the transcriptional regulatory network controlling peroxisome induction in yeast Gerrit van Meer, Nanette Kalin, Quirine Lisman, and Joost Holthuis Lipids on the move Morgan Fullerton, Laura Wagner, Zongfei Yuan, and Marica Bakovic Impaired trafficking of human CDw92/CTLI at the plasma membrane and inhibition of choline transport during monocytic cell differentiation to macrophages Mithilesh Mishra, Jim Karagiannis, Susanne Trautmann, Hongyan Wang, Mayalagu Sevugan, Volker Wachtler, Pritpal Singh, Dannel McCollum, and Mohan K. Balasubramanian Physiological role for a cytokinesis checkpoint in fission yeast M.E.K. Olsten, D.A. Canton, and D.W. Litchfield Regulation of cell morphology and the actin cytoskeleton by CKIP-1 Dmitri Satsoura, Brian Leber, David W. Andrews, and Cécile Fradin Bax oligomerization in solution and at the membrane — a fluorescence correlation spectroscopy approach James Uniacke and William Zerges Localization patterns of chloroplast mRNAs using FISH Yinan Zhang and David B. Williams The function of ERp57 in folding and assembly of MHC class | molecules Sabina Isgandarova and Gary Eitzen Mechanism of actin remodeling in association with membrane fusion Lynden Jones and Gary Eitzen Phosphorylation-mediated regulation of membrane fusion M.J. Kean, M.A. Tayeb, Q. Yi, C. Cha, and M.G. Coppolino The role of SNARE-mediated membrane trafficking in cell motility Troy Mitchell, Dalia Abdel-Latif, Gary Eitzen, and Paige Lacy The role of Rac2 in primary granule secretion from human neutrophils Johnny M. Tkach and John R. Glover Hsp!04p nucleocytoplasmic trafficking depends on active nuclear import and nuclear retention systems Troy Mitchell, Kelly Tedrick, Sabina Isgandarova, Lynden Jones, and Gary Eitzen GTPase signaling to membrane fusion: regulation of Rholp activation by the vacuolar membrane microenvironment F. Alber, B.T. Chait, D. Devos, S. Dokudovskaya, O. Karni, J. Kipper, M.P. Rout, A. Sali, C. Strambio-de-Castillia, A. Suprapto, L.M. Veenhoff, R. Williams, and W. Zhang = Structure and mechanism of the nuclear pore complex Jianrun Xia, Randy Atwal, Sanjay Anandaram, Deborah Pinchevy, Jill Taylor, and Ray Truant From homology to function: bringing the Huntington's disease protein and the karyopherins closer to the same family R.S. Atwal, D. Pinchev, S. Anandaram, A. Rezvanpour, and R. Truant Huntingtin contains a membrane-targeting signal that can affect huntingtin nuclear entry Franco J. Vizeacoumar, Wanda N. Vreden, Gary Eitzen, John D. Aitchison, and Richard A. Rachubinski The dynamin-like protein Vps|p of the yeast Saccharomyces cerevisiae associates with peroxisomes in a Pex!9p-dependent manner 2005 NRC Canada

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