Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry PDF
Preview Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry
g s.or001 2 | http://pubs.ack-1995-0619.fw ApBpiloiccahteiomMnisca asoslf aSEnpledecc tBtrroioosmtperecathryny oI loongiizcaatli on 1b er 11, 2010.1021/ ctobdoi: 2 on O1996 | 35.4y 5, 63.Ma 89.1ate: y D d bon deati ac wnloPubli Do In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. g s.or001 2 | http://pubs.ack-1995-0619.fw 1b er 11, 2010.1021/ ctobdoi: 2 on O1996 | 35.4y 5, 63.Ma 89.1ate: y D d bon deati ac wnloPubli Do In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. ACS SYMPOSIUM SERIES 619 Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry g s.or001 2 | http://pubs.ack-1995-0619.fw A. Peter Snyder, EDITOR 1b er 11, 2010.1021/ DevUe.lSo.p Amremnyt aEnddg eEwnogoinde Rereisnega rCcehn, ter ctobdoi: 2 on O1996 | 35.4y 5, 63.Ma 89.1ate: Developed from a symposium sponsored d by on D by the Division of Analytical Chemistry deati at the 209th National Meeting ac wnloPubli of the American Chemical Society, Do Anaheim, California, April 2-6, 1995 American Chemical Society, Washington, DC 1995 In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. Library of Congress Cataloging-in-Publication Data Biochemical and biotechnological applications of electrospray ionization mass spectrometry / A. Peter Snyder, editor. p. cm.—(ACS symposium series, ISSN 0097-6156; 619) "Developed from a symposium sponsored by the Division of Analytical Chemistry at the 209th National Meeting of the American Chemical Society, Anaheim, California, April 2-6, 1995." Includes bibliographical references and indexes. g s.or001 ISBN 0-8412-3378-0 12 | http://pubs.acbk-1995-0619.fw 3DM.i evI1e.Bi. st iiiSMononmngya odso(2lsefer0 csA,9pu Atenlhecas t:—lrP yote1miat9cen9aera5tl,rl : yCy1 s—9hiAs5e—nCm2a-oCih snteogrinyrm.ge . sr,Is eIICesI.ssI a.e.Al sifAm.2. )m. e rIeIiVocrnaic.ni azS anCet riChoieenhsm—e. micCaicola nSlg oSrcoeiescstieyets.y . . ctober 11, 20doi: 10.1021/ 5QQ7CP4[D 5.141N959'4L2.9.8MM.M5:—3 3 BB1d.64c 1S26p40 e c11t99r99u55m] Analysis, Mass. 2. Chromatography, Liquid. 35.42 on Oy 5, 1996 | DfoNr LLMibr/DarLyC o f Congress 95-503C81I P 63.Ma 89.1ate: This book is printed on acid-free, recycled paper. y D d bon deati Copyright © 1996 ac wnloPubli American Chemical Society Do All Rights Reserved. The appearance of the code at the bottom of the first page of each chapter in this volume indicates the copyright owner's consent that reprographic copies of the chapter may be made for personal or internal use or for the personal or internal use of specific clients. This consent is given on the condition, however, that the copier pay the stated per-copy fee through the Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923, for copying beyond that permitted by Sections 107 or 108 of the U.S. Copyright Law. This consent does not extend to copying or transmission by any means—graphic or electronic—for any other purpose, such as for general distribution, for advertising or promotional purposes, for creating a new collective work, for resale, or for information storage and retrieval systems. The copying fee for each chapter is indicated in the code at the bottom of the first page of the chapter. The citation of trade names and/or names of manufacturers in this publication is not to be construed as an endorsement or as approval by ACS of the commercial products or services referenced herein; nor should the mere reference herein to any drawing, specification, chemical process, or other data be regarded as a license or as a conveyance of any righto r permission to the holder, reader, or any other person or corporation, to manufacture, reproduce, use, or sell any patented invention or copyrighted work that may in any way be related thereto. Registered names, trademarks, etc., used in this publication, even without specific indication thereof, are not to be considered unprotected by law. PRINTED IN THE UNITED STATES OF AMERICA In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. 1995 Advisory Board ACS Symposium Series Robert J. Alaimo Cynthia A. Maryanoff Procter & Gamble Pharmaceuticals R. W. Johnson Pharmaceutical Research Institute Mark Arnold g University of Iowa Roger A. Minear 12 | http://pubs.acs.orbk-1995-0619.fw001 DUAPfnairziivveneriddr Csa iBmteyna tokBrafe olTr s Reene sneeasrscehe OAUVTnmiain&vtkce TUrea sBnrritbateya mlP lno Lea fN -acICboallhoilrnraaaoammritosopa r aiseiugsn ctober 11, 20doi: 10.1021/ RNMaoavbrayel rREte .Fs eC.a Barcsrhtae Ldllayibo,o nJr ra.t ory NUGonerivotherr gsCietay rW oolfin. M aR iScothbaigteear nUts n iversity 2 on O1996 | ChemEdit Company John R. Shapley 35.4y 5, Margaret A. Cavanaugh University of Illinois 63.Ma National Science Foundation at Urbana-Champaign y 89.1Date: Arthur B. Ellis Douglas A. Smith d bon University of Wisconsin at Madison Concurrent Technologies Corporation deati ac Downlo Publi GUnuivnedrasi tIy. oGf eKoarngsa s LDu. PSoonmt asundaram Madeleine M. Joullie Michael D. Taylor University of Pennsylvania Parke-Davis Pharmaceutical Research Lawrence P. Klemann William C. Walker Nabisco Foods Group DuPont Douglas R. Lloyd Peter Willett The University of Texas at Austin University of Sheffield (England) In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. Foreword THE ACS SYMPOSIUM SERIES was first published in 1974 to provide a mechanism for publishing symposia quickly in book form. The purpose of this series is to publish comprehensive books developed from symposia, which are usually "snapshots in time" of the current research being done on a topic, plus g s.or001 some review material on the topic. For this reason, it is neces 2 | http://pubs.ack-1995-0619.fw pstphaarreopy pe trtoBohsspea eiftdaco r trhteaea ne bae dlpx es acf ylopoumrefdr p ecscodo osbm inueatpm etprn -uebtthbhsaeli sinsises hsd ipevr bodeenvio naieoetsskw, s q eiausdoni c fpd kfuo tlhotyr te uhaa nepscrd oppselr loroesa cpcsrtrioibeiona lntetare..da ncdeStes,osd tm httoeeo 1b er 11, 2010.1021/ proaupnerd oisu tp tehere- rsecvoipewe eodf tphrei ovro ltuom fei.n aIln a acdcedpittiaonnc,e a odrra rfet joefc teioanch. 2 on Octob1996 | doi: TTerhh^eis ) aaounft ohtnhoyrems s oythumespn or seriveuivemiwse, wpthhroeoic reb sepsc aopimse ress u tphaecerc voeirdsdeitidon rgb(s yt)o to htfhe t eho err egbcaoonomizk . 63.35.4May 5, mcaemnedraat-iroenasd yo cf opbyo,t ha ntdh esu bremviietw theres fiannadl ptahpee res dtioto rths,e perdeiptoarrse, y 89.1Date: who cAhse cak rtuhlaet, aolnl lnye coersisgairnya lr erveisseiaorncsh h apvaep ebrese nan mda odrei.g inal re d bon view papers are included in the volumes. Verbatim reproduc adecati tions of previously published papers are not accepted. wnloPubli Do In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. Preface ELECTROSPRAY IONIZATION MASS SPECTROMETRY (ESI-MS) has grown as an analytical technique in exponential proportion over the past 5 or 6 years. During this time, a void has developed in the documenta tion of applications of ESI-MS for the many faceted and significant areas of the bio-related sciences. This book was developed from a series of g 012 | http://pubs.acs.or1/bk-1995-0619.pr001 ifsmccoiyaargmulls ilctittpse aistcocluuohs, dielnaelseein q c twvouhtiiifraeot o htncon o atfmnhod Etdeefe Sn rmvaIetea-sppMrslpoe,y rldSi iamsc. i raammytpi popualnlnnei-coa dasllaryotimitgoviicnepcansalle.,l, , pbimInritoto irccrbohreldpoeeubmrmecios-itcseiloooanlglntv,si ica bnanaiglo d, fl oo aaiogcnpanipdclira z oppla,ahot cibaiohnirnoetmts efa aocnochraen nult yohtati ei 1, 2102 ESI-MS is a technique that can provide significant information to 2 on October 11996 | doi: 10. soddorriscgueiaegant sytiesh-rsmee rfl aredpvoeamytn e ftcti,ht eioelbd nihso .ol aosIngtn,idc os adhrllgeoytaro ntrx,i eisftlmihactea,e tdaibon onpdo;rk oesinbm vlsemiamryoussnn mo"inlUeo ngsthtyea e;lm hpceioog!mih n-tLmpsor eooorfkcfi i lavewil e hhwsaue;tcm tayoanornd;u 35.4y 5, get when you combine an amazingly simple and user-friendly sample 3.Ma introduction system with an easy-to-operate yet very sophisticated piece 6 89.1ate: of analysis hardware. (Yes, unbelievably, there are relatively easy-to- y D operate, state-of-the-art, mass spectrometers in the commercial domain.) d bon See for yourself how seemingly complex biological samples can be han deati ac dled with ease." wnloPubli Thus one may ask how can meaningful, even ground-breaking, infor o D mation be obtained in an area as large as bio-related science with a probe that sounds too good to be true from an operator or technician's point of view. The answer is twofold. This book testifies to the fact that ESI faithfully transfers the sample, including noncovalently bound analytes, to a very powerful, but straightforward in design, MS analyzer system. A wealth of data and information can be obtained, even with as little as picomoles-femtomoles of sample, that quite often cannot be realized by other analytical systems. These two characteristics make for an ideal partnership in the exploration of biologically related phenomena and sub stances, the latter of which can have from very low to very high molecular weights in the hundreds of thousands to millions of daltons. In this book, scientists from various avenues of scientific life provide their insights on the interpretation of ESI-MS investigations. Thus, the power of this book is in the planning and methods used to resolve the xi In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. data from investigations of various biological processes into useful infor mation. This concept, presented by an interdisciplinary group of scien tists, should appeal to beginners and seasoned practitioners. Such issues as "What can I expect and what shall I look out for in my experiments" are addressed throughout the book. This makes for very useful reading by any account. Acknowledgments The seed of this project was planted by Richard R. Smardzewski. I listened to his ideas, and the result is the book you are holding in your g hands—an accounting of the power of ESI-MS challenged with one of the 012 | http://pubs.acs.or1/bk-1995-0619.pr001 mAmtBhonooeugilserIretc e lueeltclxtli,eomt pismB,r.e erTp silaiseimnn xm o Ttryehe.n vyCsti ieiRhntwiac.eeii ntsr,B g eSat htkateahepptrpeh , r eMGenmce oiaraHtanthil.uoed Csrn cW h rNatipon.a t ,Bst tu Sehrcwceeko a enpsrhcta,aia neiIsnna Ketnitds .ot A C sh. ho weBforfhwleearoidin:r h :,c uoJbrRnayimtuo,r ltieohbMsgu iaHHtcrea.k.d l 1, 2102 J. Cole, Catherine A. Costello, Robert J. Cotter, Pamela F. Crain, Jack B. 2 on October 11996 | doi: 10. DMCGouoicunuhgndaliaafefrsl,z iJ,DA .S e.H taeGanvynaeengese , R,M JMa. .cH Dakaru iDgelei .kn EH,, .HGe Gneeirrromaanclda,e n ,R C Mvo.an iDnach liHdDa eaAolln b. LaeHet.oki tG,,e sKGr,o aoFsttseht,fn lreSgieeo-nYdh r iAnJa.. b HHF Hesaiiraesrthbnisi,eb oTrin-,., 35.4y 5, William Hutchens, Constance M. John, Lawrence S. Kaminsky, Donald 3.Ma V. Kenny, Jeffrey P. Kiplinger, Steven E. Klohr, Michael D. Knierman, 6 89.1ate: Wilson B. Knight, Roger A. Laine, S. Randolph Long, Joseph A. Loo, y D James A. McCloskey, Juan Fernandez de la Mora, Melanie M. C. G. d bon Peters, James C. Richards, Kenneth L. Rinehart, Patrick A. Schindler, deati ac Jeffrey Shabanowitz, Jhobe Steadman, Justin G. Stroh, Andy J. Tomlin- wnloPubli son, Frantisek Turecek, Paul Vouros, Eric Watson, Craig M. Whitehouse, o D and Ronald N. Zuckermann. A tremendous amount of clerical and administrative assistance and organizational concepts were provided by Linda G. Jarvis, and I thank her for her accomplishments. Also, I recog nize Barbara E. Pralle for her organizational planning and support of this volume at the American Chemical Society. A. PETER SNYDER U.S. Army Edgewood Research, Development and Engineering Center Aberdeen Proving Ground, MD 21010-5423 August 22, 1995 xii In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. Chapter 1 Electrospray: A Popular Ionization Technique for Mass Spectrometry A. Peter Snyder U.S. Army Edgewood Research, Development and Engineering Center, Aberdeen Proving Ground, MD 21010-5423 g 12 | http://pubs.acs.orbk-1995-0619.ch001 ttAPThrrr yecoe oncy(medMeeaspd rSwaisn)re 1 girsi9ssoe 7 no er9f seo -ttva1hfi be9mewl 9iAase5hjmode wr dee irs rnaiacem na acdnp o l qcnSeouso miuaconlintpeteitaiztdyraa i tstwfiioovoinretnh s M at nrenaoecdsshtepsn deqSicq upftuoae etclriostt rta hofitooimevrn ete imozt rtafaayatslis o( shnAn isuopS mmneMc.be tSterhor)To m fhadoneser. d ctober 11, 20doi: 10.1021/ pni1oue9nr9mic5zeba nettAirao gSnaeMs ( EowSfS e Ilcpl)o anaapfnsee drrpes ein trwcsce eidtsnh etar esigvxeeacle tecioevtdfee esdE dS ai orIt nehp ianzataop tteeiorodfsn pi atnrele clp shaeinonrinttqieciuzduael satai.ro t, n Etah lneetced tc1 rth9hon9seip4q tr uoaaetnyasd l 35.42 on Oy 5, 1996 | cwinohcnelturedeni tnasng oa tflhy tethi cuea bbli,qo bouikiotol oaugnsid cea loelvc, etbrriovointee wciohsnn ioozfalo ttgihoiecna smlc, iebetnihotocifdhic.e mfDiieeclsdaclsr, iaepnrtievo inprorsne osmefn ethtnee d 63.Ma tal, immunological, microbiological and pharmaceutical applications of 89.1ate: ESI-tandem mass spectrometry occupy central roles. The chapters are ded by ation D tghreou inpteedrn aarlo aunndd stuhref afcoell omwoilnecgu lteosp iacns:d mtuatocrroiaml oalnecdu mleesc ohfa nbiascmtesr ioafl cEeSllIs,, wnloaPublic ndorung-cso, vadlreungt bmioemtaobloelcituelse, amssoacriinateio nto xainnsd, inmtearna-cmtioandse, ennuvcilreoinc maecnidtas,l o D contaminants, enzyme active sites, immunological processes, protein identification with database analysis, recombinant and post-translational protein investigations, and complementary protein structure and function information from ESI and matrix-assisted laser desorption/ionization (MALDI). If only Malcolm Dole could have known. That is, the great potential of a relatively simple technique known as electrospray ionization (ESI) was just that for more than a decade. But that is what the stuff of science is about. We only need to look in our own backyard for examples. Witness the length of time that it took to advance the This chapter not subject to U.S. copyright Published 1996 American Chemical Society In Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry; Snyder, A.; ACS Symposium Series; American Chemical Society: Washington, DC, 1996.
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