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Basic Methods in Antibody Production and Characterization - G. Howard, D. Bethell (CRC, 2001) WW PDF

286 Pages·2001·5.4 MB·English
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Antibody Production and Characterization BASIC METHODS IN 9445_frame_FM Page 2 Saturday, August 5, 2000 6:26 PM Edited by Gary C. Howard Castro Valley, CA Delia R. Bethell BioSeparations, Inc. Tucson, AZ Antibody Production and Characterization BASIC METHODS IN Boca Raton London New York Washington, D.C. CRC Press This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use. Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, microfilming, and recording, or by any information storage or retrieval system, without prior permission in writing from the publisher. All rights reserved. Authorization to photocopy items for internal or personal use, or the personal or internal use of specific clients, may be granted by CRC Press LLC, provided that $.50 per page photocopied is paid directly to Copyright Clearance Center, 222 Rosewood Drive, Danvers, MA 01923 USA. The fee code for users of the Transactional Reporting Service is ISBN 0-8493-9445- 7/01/$0.00+$.50. The fee is subject to change without notice. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. The consent of CRC Press LLC does not extend to copying for general distribution, for promotion, for creating new works, or for resale. Specific permission must be obtained in writing from CRC Press LLC for such copying. Direct all inquiries to CRC Press LLC, 2000 N.W. Corporate Blvd., Boca Raton, Florida 33431. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation, without intent to infringe. © 2001 by CRC Press LLC No claim to original U.S. Government works International Standard Book Number 0-8493-9445-7 Library of Congress Card Number 00-033729 Printed in the United States of America 1 2 3 4 5 6 7 8 9 0 Printed on acid-free paper Library of Congress Cataloging-in-Publication Data Basic methods in antibody production and characterization / edited by Gary C. Howard and Delia R. Bethell p. cm. Includes bibliographical references and index. ISBN 0-8493-9445-7 (alk. paper) 1. Immunoglobulins—Laboratory manuals. I. Howard, Gary C. II. Bethell, Delia R. QR186.7 .B37 2000 616.07′98—dc21 00-033729 CIP 9445_frame_FM Page 4 Saturday, August 5, 2000 6:26 PM Preface Antibodies have become indispensable tools for biomedical research. Their extraor- dinary specificity of binding is matched by their ease of use and adaptability to many experimental and now even some therapeutic applications. Basic Methods in Antibody Production and Characterization is a cookbook of methods for the production and use of antibodies. We have attempted to cover all aspects of poloyclonal and monoclonal antibodies. Many of the procedures are well established. Others (e.g., phage display) are quite new. Although we have sought to present this material in a simple step-by-step manner, no methods book can cover every experimental protocol. Each contributor has provided introductory material to give a basic understanding of the methods and references to other applications of his or her chapter’s particular technology. The methods presented are more or less in chronological order. The most impor- tant element in making antibodies is the ultimate purpose of the experiments. Time, experience, expense, and the scientific questions to be answered will direct the choice of methods for antibody production. We want to thank each of the contributors to this volume. Without their profes- sional knowledge and enthusiastic efforts, the book would not have been possible. We also thank Yale Altman for his encouragement, guidance, and unflagging support throughout the project. 9445_frame_FM Page 5 Saturday, August 5, 2000 6:26 PM 9445_frame_FM Page 6 Saturday, August 5, 2000 6:26 PM The Editors Gary C. Howard, Ph.D., received a B.A. in zoology in 1971 and an M.S. in biology from West Virginia University, Morgantown, and a Ph.D. in biological sciences from Carnegie Mellon University, Pittsburgh, PA. After fellowships at Harvard and Johns Hopkins, Dr. Howard spent 10 years with biotech companies. He edited Methods in Nonradioactive Detection (Appleton & Lange, 1993) and, with W. E. Brown, is currently preparing a volume on the practical aspects of advanced methods in protein chemistry for CRC Press. Dr. Howard is a freelance editor and writer and a senior scientific editor for the J. David Gladstone Institutes, a private biomedical research institution affiliated with the University of California, San Francisco. Delia R. Bethell, Ph.D., received a B.S. in biology from Newcomb College, Tulane University, New Orleans, LA, in 1968, an M.S. in biology from University of Denver, Denver, CO, and a Ph.D. in physiology at Hershey Medical Center, Pennsylvania State University. Dr. Bethell has worked with the production of monoclonal anti- bodies and their use in the area of in vitro diagnostics for a number of years. She has served on the Executive Board of the Society for In Vitro Biology for the past 10 years, holding the office of President from 1998 to 2000. Dr. Bethell is currently director of research with BioSeparations, Inc., in Tuscon, AZ. 9445_frame_FM Page 7 Saturday, August 5, 2000 6:26 PM 9445_frame_FM Page 8 Saturday, August 5, 2000 6:26 PM Contributors Peter Amersdorfer, Ph.D. Phylos 300 Putnam Avenue Cambridge, MA 02139

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