Basic Aspects of Receptor Biochemistry: Proceedings of the International Symposium, Vienna, Austria September 10–12, 1982

384 Pages·1983·36.309 MB·English

by  M. Goldstein, K. Jellinger, P. Riederer

Journal of Neural Transmission
Supplementum 18
Basic Aspects of
Receptor Biocheltlistry
Springer-Verlag
Wien GlDhH

Journal of Neural Transmission
Supplementum 18
Basic Aspects of
Receptor Biochentistry
Proceedings of the
International Symposium,Vienna,
September 10-12, 1982
Edited by M.Goldstein,
K. Jellinger, and P. Riederer
Springer-Verlag Wie n GmbH
Prof Dr. Menek Goldstein
Department' of Psychiatry, New York University Medical Center,
New York, N.Y., U.S.A.
Prof Dr. Kurt Jellinger
Ludwig Boltzmann-Institut flir Klinische Neurobiologie und
Neurologische Abteilung, Krankenhaus der Stadt Wien-Lainz, Wien, Austria
Prof. Dr. Peter Riederer
Ludwig Boltzmann-Institut fiir Klinische Neurobiologie
Krankenhaus der Stadt Wien-Lainz, Wien, Austria
This work is subject to copyright.
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© 1983 by Springer-Verlag Wien
Originally published by Springer-Verlag Wien-New York in 1983
Softcover reprint of the hardcover I st edition 1983
With 100 Figures
Library of Congress Cataloging in Publication Data. Main entry under title: Basic aspects
of receptor biochemistry. Oournal of neural transmission. Supplementum, ISSN 0303-6995-; 18)
"Symposium on 'Basic Aspects in Brain Receptor Biochemistry', organized by the Ludwig
Boltzmann-Institute of Clinical Neurobiology, Vienna"-P. 1. Neurotransmitter receptors
Congresses. I. Goldstein, Menek. II. ]ellinger, K (Kurt), 1931-. III. Riederer, P., 1942-.
IV. Ludwig Boltzmann-Institut ftir Klinische Neurobiologie. V. Symposium on "Basic Aspects
in Brain Receptor Biochemistry" (1982 : Vienna, Austria). VI. Series. [DNLM: 1. Receptors,
Sensory-Congresses. WI ]0781A no. 18 ! WL 102.9 B3111982]. QP364.7.B37. 1983. 599.01'88.
83-4762
ISSN 0303-6995
ISBN 978-3-7091-4410-7 ISBN 978-3-7091-4408-4 (eBook)
DOl 10.1007/978-3-7091-4408-4
Preface
In recent years a number of research fields in biology have
focussed on the concept of receptors. The selective binding of small
molecules to specific sites of the cell membrane is being increasingly
recognized as the basic mechanism by which cells communicate with
each other. It has been shown that neurotransmitters, hormones and
drugs must first interact with specific receptors in order to elicit their
biochemical, physiological, and pharmacological effects. The basic
field of "cellular receptors" and the fundamental concepts of recep
tor activity embrace a broad spectrum of biomolecular interactions
which have been the subject of intense investigations. Although con
siderable progress has been made in the understanding of the recep
tor-related phenomena, e.g. the location, sensitivity, and kinetics of
neurotransmitter receptors, little progress has been made in elucidat
ing the structures of receptors and the biochemical and biophysical
components coupled to them. Many basic problems in the function
and dysfunction of receptors and their importance in neurophysiolo
gy, neurochemistry, endocrinology, pharmacology, and in clinical
neurosciences are not yet fully understood.
It was, therefore, the object of a Symposium on "Basic Aspects in
Brain Receptor Biochemistry", organized by the Ludwig Boltzmann
Institute of Clinical Neurobiology, Vienna, as a Satellite Symposium
to the IXth International Congress of Neuropathology in Vienna,
September 1982, to provide an up-to-date overall view of the present
state of knowledge of "receptorology" with particular emphasis on
the nervous system.
Studies of different receptors have progressed at different rates
and along different lines. Some receptor-related mechanisms have
been found to play an important role in behavioural regulation,
aging, and in the pathophysiology and pathogenesis of nervous and
psychiatric disorders, e.g. of extrapyramidal diseases or schizophre
nia, and in the effects and side-effects of drug treatment. However,
since many of the fundamental mechanisms of action of receptors in
the normal and diseased nervous system are still poorly understood,
it may well be an appropriate time for compiling informations on
this special field in order to give some "crossfertilization" of ideas
VI Preface
and approaches from and to neuroanatomy, physiology, biochem
istry, molecular biology, pharmacology, behavioral and clinical
neurosciences. Thus, it was also the aim of the Symposium to seed
such an interdisciplinary interaction and to give new impacts to both
basic research and applied neurosciences. We would like to express
our gratitude to all participants of this Symposium for contributing
their efforts and for presenting the manuscripts so quickly. We are
indebted to Hoffmann-La Roche, Vienna, for supporting the organi
zation of the meeting, and to Springer-Verlag, Vienna, for providing
the rapid publication of this volume and for their appreciation of the
editorial concerns.
M. Goldstein K.}ellinger P. Riederer
Contents
Hirano, A.: The Normal and Aberrant Development of Synaptic
Structures Between Parallel Fibers and Purkinje Cell Dendritic
Spines . . . . . . . . . . . . . . . . . . . . .. 1
Arluison, A., Martres, Marie-Pascale, Sokoloff, P.: High-Resolu
tion Radioautographic Study of Dopamine Binding Sites in the
Rat Neostriatum Using 3H-Domperidone. . . . . . . .. 9
Pearson, J., Brandeis, L., Goldstein, M., Claudio Cuello, A.:
Neurotransmitter Related Immunocytochemistry of the Human
Central Nervous System . . . . . . . . . . . . . .. 25
Rausch, W.-D., Rossmanith, W., Gruber, J., Riederer, P., Jellin-
ger, K., Weiser, M.: Studies on Neurotransmitter Binding to
Pig Brain Microvessels. . . . . . . . . . . . . . .. 33
Noller, H., Kienzl, Elisabeth, Riederer, P.: Coordination Chemical
Aspects of Receptor Biochemistry. . . . . . . . . . .. 45
Franklin, T.J.: Energetics of Ligand Binding to Receptors 55
Q!lastel, D. M.J., Pennefather, P.: Receptor Blockade and Synaptic
Function . . . . . . . . . . . . . . . . . . . .. 61
Sakmann, B., Hamill, O. P., Bormann, J.: Patch-Clamp Measure
ments of Elementary Chloride Currents Activated by the
Putative Inhibitory Transmitters GABA and Glycine in Mam-
malian Spinal Neurons. . . . . . . . . . . . . . .. 83
Johnson, D.J., Wittenauer, Laura A., Nathan, R. D.: Calmodulin,
Ca2+-Antagonists and Ca2+-Transporters in Nerve and Muscle 97
Kreutzberg, G. W., Reddington, M., Lee, K. S., Schubert, P.:
Adenosine: Transport Function and Interaction with Receptors
in the CNS . . . . . . . . . . . . . . . . . . .. 113
Sulser, F., Manier, D. H., Janowsky, A.J., Okada, F.: Regulation
of Noradrenergic Receptor Systems in Brain that Are Coupled
to Adenylate Cyclase. . . . . . . . . . . . . . . .. 121
Hjorth, A., Carlsson, A., Clark, D., Svensson, K., Wikstrom, H.,
Sanchez, D., Lindberg, P., Hacksell, U., Arvidsson, L.-E.,
Johansson, A., Nilsson, J. L. G.: The Search for Selective
Dopaminergic Autoreceptor Agonists . . . . . . . . .. 131
VIII Contents
Cote, T. E., Frey, E. A., Grewe, C. W., Kebabian, J. W.: Evidence
That the D-2 Dopamine Receptor in the Intermediate Lobe of
the Rat Pituitary Gland Is Associated with an Inhibitory Cuanyl
Nucleotide Component. . . . . . . . . . . . . . . . 139
George, Susan, Binkley, Karen, Seeman, P.: Dopamine Receptor
Sites and States in Human Brain. . . . . . . . . . . . 149
Hyttel, J. Christensen, Anne Vibeke: Biochemical and Pharmaco
logical Differentiation of Neuroleptic Effect on Dopamine D-1
and D-2 Receptors. . . . . . . . . . . . . . . . . . 157
Fuxe, K., Agnati, L. F., Benfenati, F., Celani, M., Zini, I., Zoli, M.,
Mutt, V.: Evidence for the Existence of Receptor-Receptor
Interactions in the Central Nervous System. Studies on the
Regulation of Monoamine Receptors by Neuropeptides. . . 165
Cools, A. R., Oosterloo, S. K.: (3,4-Dihydroxyphenylimino)-2-Imi
dazoline (DPI) and Its Action at Noradrenergic and Dop
aminergic Receptors in the Nucleus Accumbens of Rats: Meso
limbic Catecholamine Receptors and Hyperactivity. . . . . 181
Fluckiger, E.: Interactions of Ergot Compounds with Dopamine
Receptors and Endocrine Functions. . . . . . . . . . . 189
Jenner, P., Kerwin, R., Rupniak, N. M. J., Murugaiah, K.,
Hall, M. D., Fleminger, S., Marsden, C. D.: Long-Term
Adaptive Changes in Striatal Dopamine Function in Response
to Chronic Neuroleptic Intake in Rats. . . . ... . . . . 205
Guidotti, A., Saiani, L., Wise, B. C., Costa, A.: Cotransmitters:
Pharmacological Implications . . . . . . . . . . . . . 213
Herz, A.: Multiple Opiate Receptors and Their Functional Signif-
lcance 227
Schwartz, J. C., de la Baume, Sophie, Vi, C. C., Chaillet, P.,
Marcais-Collado, Helene, Costentin, J.: Peptidases Involved
in the Inactivation of Exogenous and Endogenous Enkephalins 235
J0rgensen, O. S.: D2-Protein and D3-Protein as Markers for Synaptic
Turnover and Concentration. . . . . . . . . . . . . . 245
Goldstein, M., Engel, J., Lieberman, A., Regev, I., Bystritsky, A.,
Mino, S.: Therapeutic Potentials of Centrally Acting Dopamine
and az-Adrenoreceptor Agonists . . . . . . . . . . . . 257
Cross, A.J., Crow, T.J., Ferrier, I. N.,Johnstone, E. C., McCrea
die, R. M., Owen, F., Owens, D. G. C., Poulter, M.: Dopamine
Receptor Changes in Schizophrenia in Relation to the Disease
Process and Movement Disorder. . . . . . . . . . . . 265
Reynolds, G. P., Rossor, M. N., Iversen, L. L.: Preliminary Studies
of Human Cortical 5-HT z Receptors and Their Involvement in
Schizophrenia and Neuroleptic Drug Action . . . . . . . 273
Contents IX
Rinne, U. K., Rinne, J. 0., Rinne, J. K., Laakso, K., Laihinen, A.,
Lonnberg, P.: Brain Receptor Changes in Parkinson's Disease
in Relation to the Disease Process and Treatment . . . . . 279
Wesemann, W., Weiner, N., Rotsch, M., Schulz, E.: Serotonin
Binding in Rat Brain: Circadian Rhythm and Effect of Sleep
Deprivation . . . . . . . . . . . . . . . . . . . . 287
Riederer, P., Kienzl, E., Jellinger, K., Noller, H., Kleinberger, G.:
General Properties of 14C-L-Valine-Binding to Human Brain
Tissue . . . . . . . . . . . . . . . . . . . . . . 295
Fillion, G., Beaudoin, Dominique, Fillion, Maria-Paule, Rous
selle,J.-C., Robaut, Christine, Netter, Y.: 5-Hydroxytryptamine
Receptors in N eurones and Glia . . . . . . . . . . . . 307
Zivkovic, B., Scatton, B., Worms, P., Lloyd, K. G., Bartholini, G.:
Pharmacological and Therapeutic Actions of GABA Receptor
Agonists. . . . . . . . . . . . . . . . . . . . . . 319
McGeer, P. L., McGeer, Edith G., Nagai, T., Wong, P.-T.: A Possible
Relationship Between Folic Acid Neurotoxicity and Cholinergic
Receptors in the Pyriform Cortex and Amygdala. . . . . . 327
Sieghart, W.: Association of Proteins Irreversibly Labeled by
3H-Flunitrazepam with Different Benzodiazepine Receptors . 345
Trautmann, A.: Tubocurarine, a Partial Agonist for Cholinergic
Receptors . . . . . . . . . . . . . . . . . . . . . 353
Witzemann, V.: Differentiation-Dependent Changes of the Nico-
tinic Acetylcholine Receptor and Other Synapse-Associated
Proteins. . . . . . . . . . . . .. . . . . . . . . 363
Deguchi, T., Ohsako, S., Nakane, M., Ichikawa, M., Yoshioka, M.:
Cellular Mechanism Involved in the Synthesis of Cyclic GMP
in Nervous Tissues . . . . 369
Goldstein, M.: Closing Remarks. 379
Subject Index. . . . . . . . 381
J. Neural Transmission, SUpp!. 18, 1-8 (1983)
The Normal and Aberrant Development of Synaptic
Structures Between Parallel Fibers and
Purkinje Cell Dendritic Spines
A.Hirano
Division of Neuropathology, Department of Pathology, Montefiore Hospital and
Medical Center, Albert Einstein College of Medicine, Bronx, N. Y., U.S.A.
With 4 Figures
Summary
Under normal circumstances the dendritic spines of the cerebellar
Purkinje cell differentiate in association with the parallel fibers formed by
the descending granule cells. In the adult these elements form the most
frequent synapse of the cerebellar cortex. In certain conditions, however, the
granule cells are destroyed before they form the parallel fibers. Nevertheless,
unattached dendritic spines are found which are complete with submem
branous densities. Their cytochemical reactions and their morphology in
both thin section and after freeze fracture are indistinguishable from normal
spines except for the fact that they are unattached to any presynaptic ele
ments. Examples of the formation of unattached presynaptic endings have
also been observed. We conclude, therefore, that at least in some instances,
pre-and postsynaptic terminals may form without benefit of the direct one
to-one influence of their synaptic mates.
The architecture of the cerebellum is better understood than
most other areas of the central nervous system (Palay and Chan
Palay, 1974). It therefore seemed a good choice for the study of
synaptic development, especially for the interaction of granule cells
and Purkinje cells which are the major neurons of the cerebellar
cortex.
Early in development, the immature granule cells occupy the ex
ternal germinal layer while the Purkinje cells are confined to a deeper
level. As the granule cells descend into the molecular layer the
1 Journal of Neural Transmission, Suppl.18