Atlas of Human Reproduction: By Scanning Electron Microscopy

336 Pages·1982·26.27 MB·English

by  P. Kenemans

Published in the UK and Europe by
MTP Press Limited
Falcon House
Lancaster, England
British Library Cataloguing in Publication Data
Atlas of human reproduction
1. Human reproduction-Congresses
I. Hafez, E.S.E. 11. Kenemans, P.
612'.6 QP251
Published in the USA by
MTP Press
A division of Kluwer Boston Ine
190 Old Derby Street
Hingharn, MA 02043, USA
Library of Congress Cataloging in Publication Data
Main entry under title:
Atlas of human reproduction by scanning electron
microseopy.
Bibliography: p.
Includes index.
1. Generative organs-Atlases. 2. Human
reproduction-Atlases. 3. Scanning electron
microseope. I. Hafez, E.S.E., 1922-
11. Kenemans, P.
QM557.A84 1982 611'.6'0222 82-14003
ISBN 978-94-011-8142-6 ISBN 978-94-011-8140-2 (eBook)
DOI 10.1007/978-94-011-8140-2
Copyright © 1982 MTP Press Limited
Softcover reprint of the hardcover 1st edition 1982
All rights reserved. No part of this publication may be
reproduced, stored in a retrieval system, or transmitted in any
form or by any means, electronie, meehanical, photocopying,
recording or otherwise, without prior permtssion from the
publishers.
Typeset by Peter Whatley/Anneset, Trowbridge and
Weston-super-Mare
CONTENTS
Contributors VII
Foreword Tom Eskes Xl
Preface X111
Selected general references XV
1 Specimen preparation techniques P. Kenemans, V. P. Colling and U. Brunk 1
2 Tissue organization and reproduction E. S. E. Hafez and P. Kenemans 7
I. GYNECOLOGY
3 The vagina (normal) E. S. E. Hafez and P. Kenemans 15
4 The vagina (pathology) R. W. de Haan, W. N. P. Willemsen,
C. P. Vooys, E. S. E. Hafez and
P. Kenemans 29
5 The Bartholin gland M. Murikami,J. Abe and T. Nishida 37
6 The cervix P. Kenemans,J. Davina, R. W. de Haan
and E. S. E. Hafez 45
7 Cervical mucus B. Daunter and P. Lutjen 55
8 Postovulatory endometrium P. Sundstrom and B. o. Nilsson 61
9 Endometrial tumors F. Stenback, M. Oshima, K. Ida,
H. Okamura and A. Kauppila 71
10 Response of postmenopausal endometrium W. H. Wilborn, C. E. Flawers"fr.And
to hormonal therapy .-R. M. Hyde 95
11 Effects of IUDs on the endometrium H. H. El-Badrawi and E. S. E. Hafez 101
12 Uterine cervical and endometrial cells S. Nozawa, l. Ishiwata, 5. Tlfguchi,
in vitro: can reserve cells grow in vitro? S. Tsukahara, S. Kurihara and H. Okumura 111
13 The fallopian tube in infertility C. Vasquez, I. A. Brosens, S. Cordts,
W. Boeckx and R. L. M. Winston 119
14 Fetal ovary S. Makabe and P. M. Motta 129
15 The ovary and ovulation S. Makabe, E. S. E. Hafez and P. M. Motta 135
16 Ovarian tumors F. Stenback, S. Makabe, C. Omura,
A. Iwaki and E. S. E. Hafez 145
17 The mammary gland E. Spring-Mills, M. O. Brophy and
P.J.Numann 159
v
vi
CONTENTS
II. ANDROLOGY
18 The seminal vesicle E. Spring-Mills and M. Bush 169
19 The vas deferens and seminal coagulum L.J. D. Zaneveld, P. F. Tauber
and E. E. Brueschke 173
20 The vas deferens in man and monkey; M. Murakami, A. Sugita and
spermiophagy in its ampulla M.Hamasaki 187
21 Spermatozoa B. Baccetti, E. S. E. Hafez and K. G. Gould 197
22 Spermophagy J. K. Koehler, R. E. Berger, D. Smith and
L.E.Karp 213
23 Sperm cell-cervical mucus interaction F. C. Chretien 219
III. CONCEPTUS
24 Interaction between spermatozoa and ovum
in vitro . P. Sundstrom 225
25 The normal placenta H. P. van Geijn, M. Castellucci,
P. Kaufmann and P. Kenemans 231
26 The pathological placenta P. Kenemans, H. P. van Gei;n and
E. S. E. Hafez 246
27 Amniotic fluid cells and placental membranes o. Tyden 255
28 Human embryo and fetus R. E. Waterman 261
29 Hydatidiform mole U. M. Spornitz and E. S. E. Hafez 275
IV. SPECIAL TECHNIQUES
30 X-ray microanalysis G. M. Roomans 287
31 Cell sudace markers and labeling techniques R.S.Molday 297
32 Animal models for SEM studies on
cervical carcinogenesis C.A. Rubio 305
V.EPILOGUE
33 Clinical application of SEM to human T. K. A. B. Eskes, E. S. E. Hafez and
reproduction P.Kenemans 313
34 Diagnostic applications to oncology G. M. HodgesandP. Kenemans 325
35 SEM technology, parameters and
interpretations E. S. E. Hafez and P. Kenemans 339
Subject index
Contributors
Abe,J. Brunk, U.
Department of Anatomy, Institute of Pathology,
Kurume University School of Medicine, University of Linkoping,
Kurume, Linkoping,
Japan Sweden
Brueschke, E. E.
Baccetti, B.
Department of Family Practice,
Universita di Siena,
Rush Medical School,
Istituto de Zoologia,
Via Mattioli 4, Chicago,
53100 Siena, Illinois,
Italy USA
Bush,M.
Berger, R. E.
Department of Anatomy and Urology,
Department of Biological Structure,
State University of New York,
Urology and Obstetrics/Gynecology,
Upstate Medical Center,
University of Washington,
Syracuse,
School of Medicine,
New York 13210,
Seattle,
USA
Washington 98195,
USA
Castellucci, M.
Boeckx, w. Abt. Elektronenmikroskopie,
The Unit for the Study of Human Reproduction, der Medizinischen Hochschule,
Catholic University, Karl-Wiechert-Allee 9,
Leuven, 3000 Hannover 61,
Belgium West Germany
Chretien, F. C.
Boyde, A.
Travaux Pratiques de Biologie Animale du PCEM,
Anatomy Department,
12, rue Cuvier,
University College of London,
75005,
Gower Street,
Paris,
London WCIE 6BT,
France
United Kingdom
o. Collins, V. P.
Brophy,
Institute of Tumor Pathology,
Departments of Anatomy and Urology,
Karolinska Institute,
State University of New York,
Stockholm,
Upstate Medical Center,
Sweden
Syracuse,
New York 13210,
Daunter, B.
USA
Department of Obstetrics/Gynecology
Brosens, I. A. University of Queensland, '
The Unit for Study of Human Reproduction, Clinical Sciences Building,
Catholic University, Royal Brisbane Hospital,
Leuven, Q 4029,
Belgium Australia
Vll
viii CONTRIBUTORS
Davina,H. Hamasaki, M.
Institute for Submicroscopic Morphology, Department of Anatomy,
University of Nijmegen Medical School, Kurume University School of Medicine,
6525 GA Nijmegen, Kurume,
The Netherlands Japan
Hodde, K. C.
EI-Badrawi, H. H.
Laboratory for Surgical Research,
24 Falah St,
Wilhelmina Gasthuis University of Amsterdam,
Madinet EI-Mohandsin,
Amsterdam,
Giza, Cairo,
The Netherlands
Egypt
Hodges, G. M.
Eskes, T. K. A. B.
. Imperial Cancer Research Fund,
Department of Gynecology/Obstetrics,
PO Box 123,
University of Nijmegen,
Lincolns Inn Field,
PO Box 9101,
London WC2A 3PX,
6500 HB Nijmegen,
United Kingdom
The Netherlands
Hyde,B. M.
Flowers, C. E. Jr Department of Anatomy,
Department of Obstetrics/Gynecology, University of South Alabama,
University of Alabama, Mobile,
Birmingham, Alabama 36608,
Alabama 36688, USA
USA
Ida, K.
van Geijn, H. P.
Department of Obstetrics/Gynecology,
Department of Obstetrics/Gynecology,
Shiga University of Medical Sciences,
University of Amsterdam,
Shiga,
Free University,
Japan
Amsterdam,
The Netherlands
Ishiwata, I.
Department of Obstetrics/Gynecology,
Gordts, S.
School of Medicine,
The Unit for the Study of Human Reproduction,
Keio University,
Catholic University,
35 Shinanomachi,
Leuven,
Shinjukuru,
Belgium
Tokyo 160,
Japan
Gould,K. G.
Division of Reproductive Biology, Iwaki,A.
Yerkes Regional Primate Research Center, Department of Obstetrics/Gynecology,
Emory University, T oho University,
Atlanta, School of Medicine,
Georgia 30322, Tokyo 143,
USA Japan
Karp, L. E.
Haan, R. W. de
Department of Biological Structure,
Department of Obstetrics/Gynecology,
Urology and Obstetrics/Gynecology,
University of Nijmegen,
University vfWashington,
Nijmegen,
School of Medicine,
The Netherlands
Seattle,
Washington 98195,
Hafez, E. S. E. USA
Department of Gynecology/Obstetrics,
School of Medicine, Kaufmann, P.
Wayne State University, Department of Anatomy,
550 East Canfield, University of Hamburg,
Detroit, School of Medicine,
Michigan 48201, Hamburg,
USA West Germany
CONTRIBUTORS IX
Kauppila, A. Murakami, M.
Department of Obstetrics/Gynecology, Department of Anatomy,
University of Oulu, Kurume University School of Medicine,
Kajaanintie 52 D, Kurume,
SF-90220 Oulu 22, Japan
Finland
Nilsson, B. O.
Kenemans, P. Reproduction Research Unit,
Department of Gynecology/Obstetrics, Biomedical Center,
University of Nijmegen, PO Box 571,
PO Box 9101, 75123 Uppsala,
6500 BH Nijmegen, Sweden
The Netherlands
Nishida, T.
Department of Anatomy,
Koehler, J. K. Kurume University School of Medicine,
Department of Biological Structure,
Kurume,
University of Washington School of Medicine,
Japan
Seattle,
Washington 98195, Nozawa, S.
USA Department of Obstetrics/Gynecology,
School of Medicine,
Kurihara, S. Keio University,
Department of Obstetrics/Gynecology, 35 Shinanomachi,
School of Medicine, Shinjukuru,
Keio University, Tokyo 160,
35, Shinanomachi, Japan
Shinjukuru,
Tokyo 160, Numann, P. J.
Japan Department of Anatomy and Urology,
State University of New York,
Upstate Medical Center,
Lutjen, P. J.
766 Irving Avenue,
Department of Obstetrics/Gynecology,
Syracuse,
University of Queensland,
New York 13210,
Clinical Sciences Building,
USA
Royal Brisbane Hospital,
Herston 4029,
Okamura,H.
Queensland,
Department of Virology and Rickettsiology,
Australia
National Institute of Health,
Kamiosaki,
Makabe, S. Shinagawaku,
Department of Obstetrics/Gynecology, Tokyo 141,
Toho University School of Medicine, Japan
11-1 Omoriniski 6 Chome,
IKA-TU, Omura,G.
Tokyo 143, Department of Obstetrics/Gynecology,
Japan Tokyo University School of Medicine,
Tokyo 143,
Japan 606
Molday, R. S.
Department of Biochemistry, Oshima,M.
University of British Columbia, Department of Obstetrics/Gynecology,
2075 Westbrook Mall, Shiga University of Medical Sciences,
Vancouver, T uskinowa-Cho-Seta,
British Columbia, Otsu-City,
Canada V6T 1W5 Shiga-Pref.,
Japan 520-521
Motta, P. M. Roomans, G. M.
Instituto di Anatomia Umana Normale, Wenner-Gren Institute,
Universita di Roma, University of Stockholm,
Viale Regina Elena, Norrtullsgatan 16,
289-00161, Rome, S-11345 Stockholm,
Italy Sweden
x CONTRIBUTORS
Rubio, C. A. Tsukahara, S.
Department of Pathology, Department of Obstetrics/Gynecology,
Karolinska Sjukhuset, School of Medicine,
S-10401, Stockholm 60, Keio University,
Sweden Tokyo 160,
Japan
Smith, D.
Departments of Biological Structure, Tyden, O.
and Obstetrics/Gynecology, Department of Obstetrics/Gynecology and Anatomy,
University of Washington School of Medicine, University of Uppsala,
Seattle, 5075014 Uppsala,
Washington 98195, Sweden
USA
Vasquez, G.
The Unit for the Study of Human Reproduction,
Spomitz, U. M.
Catholic University,
Anatomisches Institut
Leuven,
der Universitiit Basel,
Belgium
Pestalozzistrasse 20,
CH 4056 Basel,
Switzerland Vooys,G. P.
Department of Pathology,
University of Nijmegen,
Spring-Mills, E.
6525 GA,
Departments of Anatomy and Urology,
Nijmegen,
State University of New York,
The Netherlands
Upstate Medical Center,
766 Irving Avenue,
Syracuse, Waterman, R. E.
New York 13210, Department of Anatomy,
USA School of Medicine,
University of New Mexico,
Albuquerque,
Stenback, F.
New Mexico 87131,
Department of Pathology,
USA
University of Oulu,
Kajaaintie 52D SF 90220,
Oulu 22, Wilborn, W. H.
Finland Department of Anatomy,
University of South Alabama,
Mobile,
Sugita, A.
Alabama 36688,
Department of Anatomy,
USA
Kurume University School of Medicine,
Kurume,
Willemsen, W. N. P.
Japan
Department of Gynecology/Obstetrics,
University Hospital,
Sundstrom, P. Sint Radboud,
Department of Obstetrics/Gynecology, PO Box 9101,
Malmo Allmanna Sjukhus, 6500 BH Nijmegen,
S-214 01 Malmo, The Netherlands
Sweden
Winston, R. L. M.
Taguchi, S. Hammersmith Hospital,
Department of Obstetrics/Gynecology, London,
School of Medicine, England
Keio University,
Tokyo 160, Zaneveld, L. J. D.
Japan
Department of Physiology, Biophysics and Obstetrics/
Gynecology ,
Tauber, P. F. College of Medicine,
Department of Obstetrics/Gynecology, University of Illinois At the Medical Center,
University of Essen, Chicago,
Essen, Illinois 60680,
West Germany USA
Foreword
The suggestion of Max Knoll that an electron fascinated by the numerous SEM photographs, the
microscope could be developed using a fine scanning wealth of information and the enthusiasm of the
beam of electrons on a specimen surface and recording researchers covering a variety of disciplines. All aspects
the emitted current as a function of the position of the of the female and male genital tract have been covered,
beam was launched in 1935. Since then several culminating in the prizewinning award showing the in
investigators and clinicians have used this concept to vitro fertilized human egg.
develop techniques now known as scanning electron In clinical diagnostics SEM also proved to be a
microscopy (SEM) and scanning transmission electron valuable complementary technique, shedding new light
microscopy (STEM). The choice to study the female on oncology, the pathogenesis of tubal disease and the
reproductive organs was a logical one because cells and maturation process of the placenta. Future research has
tissue samples can be sampled relatively easily; still to be accomplished; e.g. quantification of SEM
furthermore, these cells and organs are influenced photographs for meaningful and sound biological,
continuously by the cyclic production of hormones. scientific and statistical evaluation in diagnostic
This atlas demonstrates the state of the art in 1983. gynecology, obstetrics, andrology and oncology.
Having such predecessors as Mammalian Re If this atlas does encourage the investigators in the
production and The Human Female Reproductive field and their offspring to take up this challenge, the
Tract one can judge the progress made in techniques numerous people who used their 'electrons' to make the
and their application. The basis for this research was Nijmegen SEM Symposium so successful, and this atlas
laid during an international SEM Symposium, 'Human possible, will be completely satisfied.
Reproduction in Three Dimensions', held in Nijmegen,
The Netherlands, in September 1981. As one of the
organizers, and especially not a morphologist, I was Nijmegen, October 1982 TOM ESKES
Xl