Asthma M E T H O D S I N M O L E C U L A R M E D I C I N ETM John M. Walker, SERIES EDITOR 19. Hepatitis C Protocols, edited by Johnson Yiu-Nam Lau, 1998 18. Tissue Engineering, edited by Jeffrey R. Morgan and Martin L. Yarmush, 1998 17. HIV Protocols, edited by Nelson Michael and Jerome H. Kim, 1998 16. Clinical Applications of PCR, edited by Y. M. Dennis Lo, 1998 15. Molecular Bacteriology: Protocols and Clinical Applications, edited by Neil Woodford and Alan Johnson, 1998 14. Tumor Marker Protocols, edited by Margaret Hanausek and Zbigniew Walaszek, 1998 13. Molecular Diagnosis of Infectious Diseases, edited by Udo Reischl, 1998 12. Diagnostic Virology Protocols, edited by John R. Stephenson and Alan Warnes, 1998 11. Therapeutic Application of Ribozymes, edited by Kevin J. Scanlon, 1998 10. Herpes Simplex Virus Protocols, edited by S. Moira Brown and Alasdair MacLean, 1998 9. Lectin Methods and Protocols, edited by Jonathan M. Rhodes and Jeremy D. Milton, 1998 8. Helicobacter pylori Protocols, edited by Christopher L. Clayton and Harry L. T. Mobley, 1997 7. Gene Therapy Protocols, edited by Paul D. Robbins, 1997 6. Molecular Diagnosis of Cancer, edited by Finbarr Cotter, 1996 5. Molecular Diagnosis of Genetic Diseases, edited by Rob Elles, 1996 4. Vaccine Protocols, edited by Andrew Robinson, Graham H. Farrar, and Christopher N. Wiblin, 1996 3. Prion Diseases, edited by Harry F. Baker and Rosalind M. Ridley, 1996 2. Human Cell Culture Protocols, edited by Gareth E. Jones, 1996 1. Antisense Therapeutics, edited by Sudhir Agrawal, 1996 M E T H O D S I N M O L E C U L A R M E D I C I N ETM Asthma Mechanisms and Protocols Edited by K. Fan Chung and Ian Adcock Imperial College School of Medicine, London, UK Humana Press Totowa, New Jersey © 2000 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular Medicine™is a trademark of The Humana Press Inc. All authored papers, comments, opinions, conclusions, or recommendations are those of the author(s), and do not necessarily reflect the views of the publisher. This publication is printed on acid-free paper. ∞ ANSI Z39.48-1984 (American Standards Institute) Permanence of Paper for Printed Library Materials. Cover illustration: Cover design by Patricia F. Cleary. For additional copies, pricing for bulk purchases, and/or information about other Humana titles, contact Humana at the above address or at any of the following numbers: Tel.: 973-256-1699; Fax: 973-256-8341; E-mail: [email protected]; Website: http://humanapress.com Photocopy Authorization Policy: Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Humana Press Inc., provided that the base fee of US $10.00 per copy, plus US $00.25 per page, is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923. For those organizations that have been granted a photocopy license from the CCC, a separate system of payment has been arranged and is acceptable to Humana Press Inc. The fee code for users of the Transactional Reporting Service is: [0-89603-626-X/00 $10.00 + $00.25]. Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1 Library of Congress Cataloging in Publication Data Main entry under title: Methods in molecular medicine™.