asian-pacific aquaculture 2017

405 Pages·2017·56.51 MB·English

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ASIAN-PACIFIC AQUACULTURE 2017
Transforming For Market Needs
Putra World Trade Centre
July 24-27, 2017 • Kuala Lumpur, Malaysia
Hosted by: Department of Fisheries, Ministry of Agriculture & Agro-Based Industry, Malaysia
Gold Sponsor President Reception Sponsor Session Sponsors
WAS Premier Sponsors
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WELCOME TO ASIAN-PACIFIC AQUACULTURE 2017
Selamat Datang and Welcome
On behalf of the Department of Fisheries, Malaysia (DOF)
and the Steering Committee, it is my pleasure to welcome all
participants and industry professionals to Kuala Lumpur and to
the Asian Pacific Aquaculture 2017. This is the second time that
DOF is hosting this leading Asian-Pacific aquaculture industry
event in Kuala Lumpur. The theme of this year’s APA 2017
is “Transforming for Market Needs.” This is the way forward
for the region’s industry; market-driven production of fish and
marine shrimp and in a sustainable manner.
We are very proud that the Asian Pacific Chapter of the World Aquaculture Society has again
chosen Malaysia for this annual event. We hosted APA in 2009 and since then, there have been rapid
developments and changes in Malaysia’s aquaculture industry. Many involved in fish and shrimp
farming will realize that the recent years have been challenging times, in particular with diseases.
Together at this conference, I envisage that there will be active knowledge sharing to help all of us
overcome these challenges. DOF together with Malaysian stakeholders look forward to an exciting
exchange of knowledge and information among local and foreign participants. Of particular interest to
local farmers, is the Malaysian Farmers Day where we will discuss the transformation of its aquaculture
towards 2020.
DOF and the Ministry of Agriculture and Agro Based Industry have developed a transformation
program for some segments of industry in Malaysia. We will require advancement of technologies and
technical expertise. We welcome interactions at the conference and trade show to help us achieve our
targets.
I wish you a successful conference and trade show. However, while you focus on the conference, I urge
our foreign participants to also explore the hospitality, culture and beauty of Malaysia.
Thank you
DATUK HAJI ISMAIL BIN ABU HASSAN
Director General, Department Of Fisheries Malaysia
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TABLE OF CONTENTS
W .......................................................................2
elcome
A -P A 2017 A ...............5
siAn Acific quAculture bstrActs
A .................................................................403
ddendum
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ABSTRACTS
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MODERN INDUSTRIAL GROUPER FARMING IN LANGKAWI, MALAYSIA
Mohd Addin Aazif
Chief Operation Officer
Fishance Berhad, Langkawi, Malaysia
No.31, 1st Floor, Baron Bandar Baru
Persiaran Putra, 07000, Kuah, Langkawi
Kedah Darul Aman
[email protected]
Fishance Berhad is a newly-established aquaculture business specializing in indoor hatchery and cage system fish farming.
Based in Langkawi, Malaysia, the pristine waters surrounding the group of islands is ecologically suitable for farming of
high value species like hybrid groupers (tiger grouper, Epinephelus fuscoguttatus x giant grouper, Epinephelus lanceolatus),
cantik grouper (camouflage grouper, Epinephelus polyphekadion x tiger grouper, Epinephelus fuscoguttatus), orange
spotted grouper, Epinephelus coioides and other aquaculture commercially-potential species.
The groupers are the major cultured species as their advantages of hybrid vigor which improved the species by strengthening
the progeny, generating healthier and high resistance species as well as better yield, faster growth, high prices, improved
quality, hardy and stronger breed that contributes to superior economic profitability. Fishance Berhad’s commitment to
the modern grouper farming is by constructing 10,000 conventional cages and HDPE round cages which will be located
in Langkawi’s natural, clean and strategic waters. Grouper seed production will be based on a world-class marine fish
hatchery in Tuba Island which consists of brood stock rearing facilities, live feed culture area, larviculture and grow out
tanks, HDPE liner earth ponds complete with an advance diagnostic disease laboratory. Grouper seed production also
guarantees sustainability, either for our own use or for commercialization.
The participation of Fishance Berhad in the modern farming will definitely create a new dynamic dimension on the
country’s aquaculture industry.
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THE EFFECT OF FEEDING PRIMEZEAT® ON HEALTH STATUS OF Penaeus monodon
AND PERFORMANCE OF Macrobrachium rosenbergii BROODSTOCK
Mohammed Suhaimee A. Manaf*, Rosnani Yaakub, Nazariah Nazri and Nur Awatif Othman
Fisheries Research Institute Pulau Sayak
08500 Kota Kuala Muda, Kedah, Malaysia
[email protected]
At present, most of shrimp (Penaeus sp.) and prawn (Macrobrachium rosenbergii) hatchery operators still rely on fresh food
such as squid, fish, shellfish and polychaete as feed for broodstock. However, there are risks of disease transmission such
as parasites, bacteria and viruses from fresh food. Although imported shrimp broodstock maturation feeds are available,
yet costly and not easy to use. Therefore, an innovative disease-free moist feed for shrimp broodstock called PrimEZeat®
was developed. PrimEZeat® is available in frozen form and is very easy to use. There were two feeding trials conducted to
evaluate the effect of feeding PrimEZeat® and fresh feed on the health and performance of shrimp (P. monodon) and prawn
(M. rosenbergii) broodstock.
In Trial-1, a total of 30 pairs of wild tiger shrimp (P. monodon) broodstock with an average weight of 64.3 ± 15.2g (male)
and 103.9 ± 13.4g (female) were stocked in six, 5MT tanks at 5 pairs/tank with the sex ratio ( ♂: ♀) of 1:1. The initial qPCR
screening found that 50% of samples were infected with IHHNV, 80% with WSSV, and 60% with AHPND. Three tanks
were assigned with fed fresh squid and commercial pellets (D1) compared with another three tanks with PrimEZeat® and
commercial pellets. Wilcoxon comparative analysis of virus presence indicated that all tiger shrimp broodstock were found
to be free from virus (Z = -2,558, p = 0.011) on Week-24 compared to Week-1. While Independent Samples T-Test analysis
on the survival rate (mean ± SD%) showed no significant difference (P>0.05) between tiger shrimp broodstock fed D1
(60.0 ± 15.5%) and PrimEZeat® (50.0 ± 17.9%).
In Trial-2, a total of 120 female prawn broodstock (average weight = 28.1 ± 5.8g) stocked with 40 male prawn broodstock
(average weight = 39.6 ± 10.1g) in four 10MT tanks with 1: 3 ratio (♂: ♀) per tank. Fresh squid, cockle meat and commercial
pellets (D1) and PrimEZeat® with commercial pellets were fed to prawn broodstock at a rate of 7.5%/day. The t-test analysis
on the performance of prawn broodstock for two months (TABLE 1) showed no significant difference (P>0.05) between
prawn broodstock fed with D1 and PrimEZeat® in terms of final weight (g), survival rate (%), egg weight (g), number of
naupli/broodstock, number of naupli/g broodstock and hatching rate (%).
These trials proved that PrimEZeat® had no negative impact on the health, survival rate of tiger shrimp broodstock and
the performance of prawn broodstock. Consider the risk of disease, food quality and operating costs suggest that prawn
or shrimp hatchery operators may change from using fresh feed to a disease-free formulated moist pellet PrimEZeat® as
broodstock feed.
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HISTOLOGICAL STUDIES OF THE OOGENESIS AND MATURATION OF THE PORTUNID
CRAB Portunus pelagicus (L.) IN EGYPTIAN WATERS
F. A. AbdelRazek*; M. Ismaiel; A. Khafagy; J. Sorour; A. Attia
National institute of Oceanography and Aquaculture, Invertebrate Laboratory, Alexandria, Egypt
Email address: [email protected]
Abstract
This study is concerned with understanding the process of reproduction and the changes happening in the ovary of Portunus
pelagicus during maturation, which would be useful for its broodstock development for hatchery purposes. Results showed
that, the ovarian development stages of P. pelagicus females were divided into five stages: immature; early maturing;
maturing; ripe and spent.
Introduction
Marine blue swimmer crab Portunus pelagicus is considered the most economical established invasive alien crab species
in Egypt, The studies on the structural details of reproductive stages form the base knowledge to perceive the process of its
reproduction, which in turn is the primary step toward developing the hatchery technology of P. pelagicus in Egypt.
Material and Methods:
Live female specimens of P. pelagicus collected
from Egypt, Alexandria Mediterranean coasts
during March 2015-February 2016, and dissected
in order to determine the gonad development stages
according to Ravi et al. (2013). Gonad lobes were
fixed in Davidson solution, then preserved into
the 70% alcohol solution, after that dehydrated
and moved into cedar oil, embedded in paraffin.
Sectioning was at 5 μm, then mounted, and stained
with H&E, then examined with Olympus CX 31
light microscope.
Results and Discussion:
The ovarian maturation stages during one
reproductive cycle have been classified into five
stages included A- Immature stage: Ovary
comprised of three different kinds of cells; Og,
Oc I and Oc II. Og mean cell diameter were about
11 µm, they are localized in at the center of each
follicle moved toward the periphery of the ovary as
they grow. Oc I mean cell diameter were about 39
µm. their cytoplasm appeared with highly distinct
blue color, while Oc II mean cell diameter were
about 57 µm. The cytoplasm was blue and had
eosinophilic yolk granules. Supporting follicular cells are present adjacent to Oc I & II (Fig.1.A). B- Early maturing
stage: ovaries characterized by the appearance of Oc III. Which had a mean cell diameter about 92 µm. The cyoplasm was
slightly eosinophilic. The follicular cells were also found indicating a special microenvironment for them, (Fig.1.B).C-
Maturing stage: Oc III were predominant, as they mature to form Oc IV. The developing cells from the germinal zone
invaded and formed extension of germinal zone called “germinal nests”, this was also observed in the study of Ravi et al.
(2013), (Fig.1.C). D- Ripe stage: Ovary was filled with Oc IV (Fig.1.D), which had a mean cell size of 156 µm, where size
increased as yolk was developed into the cytoplasm, these results are in agreement with the work of Islam et al. (2010) of
the mud crab Scylla paramamosain. The cytoplasm was highly eosinophilic and granular. Lumen and germinal zone were
completely absent. E- Spent stage: “spawning” stage, also termed as ‘resting’ as it was reported by (Fondo et al., 2010),
after which most of the completely mature and viable eggs have been released from the ovary. At this stage, the ovary
had a large number of spent⁄resorbing oocytes. Visible degeneration of the oocytes occurs, then oogenesis is reinitiated
immediately, (Fig.1.E).
(Continued on next page)
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References:
1-Fondo, E.N., Kimani, E.N. and Odongo, D.O., 2010. The status of mangrove mud crab fishery in Kenya, East Africa.
International Journal of Fisheries and Aquaculture, 2(3), pp.079-086.
2-Islam, M.S., Kodama, K. and Kurokora, H. 2010. Ovarian development of the mud crab Scylla paramamosain in a
tropical mangrove swamps, Thailand. Journal of Scientific Research, 2(2), pp.380-389.
3-Ravi, R., Manisseri, M.K., and Sanil, N.K., 2013. Ovarian maturation and oogenesis in the blue swimmer crab, Portunus
pelagicus (Decapoda: Portunidae). Acta Zoologica, 94(3), pp. 291-299.
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COMPARATIVE HISTOMORPHOLOGY OF DIGESTIVE SYSTEM AND ENZYMES IN
Johnius belangerii
Rahim Abdi*, Saeed Hasanzadeh, Mohammadali Salarialiabadi, Abdulali Movahedinia, Zahra Basir
*Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science
and Technology
E. Mail Address: [email protected]
Johnius belangerii have high economic value in the Persian Gulf. In this research the differences in histological and
enzymatic of digestive tract in Johnius belangerii as a carnivore diet was studied. For this purpose, 10 fish were collected
from the beach of Persian Gulf. Then, tissue samples from first and end of the esophagus , stomach and intestine along
with middle of intestine were carried out. Also for enzyme samples the first and end of the stomach and intestine along
with middle of the intestine were removed from the gastrointestinal tract. Rutine procedure of tissue preparation including
fixation, dehydration with ethanol, clearation with xylene, blocking with paraffin and cutting with microtome as a thickness
of 5 microns were done. After clearing of slides, studied by light microscope. Results showed the length of intestinal villi in
the first part of the intestine was more than other parts (p≤0.05). Also intestinal mucosal cells increased from the primary
to the end. There was no significant difference in histological observation. In enzyme samples the activity of the amylase
that breaks down carbohydrates in food and alkaline phosphatase activity was high in other species more than Johnius
belangerii (p≤0.05). Also lipase activity that breaks down lipids in Johnius belangerii was higher than from species that
reported by some researchers (p≤0.05).
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EFFECT OF MICROALGAE-ASSOCIATED BACTERIA ON THE GROWTH OF Botryococcus
braunii Kϋtzing 1849
Muhamad A.R. Ridzuan1, *, Fatimah Md. Yusoff1, 2, Yam Sim Khaw1, and Fatin M.I. Natrah1, 2
1Laboratory of Marine Biotechnology
Institute of Bioscience
Universiti Putra Malaysia
43400 UPM Serdang
Selangor, Malaysia
2Department of Aquaculture
Faculty of Agriculture
Universiti Putra Malaysia
43400 UPM Serdang
Selangor, Malaysia
[email protected]
Microalgae have high commercial value in addition to their crucial role in providing energy in the aquatic food chain.
They are valuable sources of various compounds such as antioxidants, fatty acids and amino acids that can be utilized in
food, nutraceutical, pharmaceutical, and aquaculture industries. Among many microalgae species, Botryococcus braunii
is well known for its high lipid content and its potential application in renewable energy industry. However, its slow
growth rate is a major hindrance to be utilized commercially. This study aimed to examine the bacterial community and
its interaction with Botryococcus braunii in influencing the growth of latter. The Botryococcus braunii (UPMC-A0021)
was isolated and purified from Putrajaya Lake, Malaysia, and its identity was confirmed based on its morphology (light
and scanning electron microscope) and DNA sequences (PCR method). Symbiotic bacteria were isolated and identified
from the microalga-growing culture. Each isolated bacteria was added into an axenic microalgae culture respectively to
examine the effect of bacteria on the microalga growth. Nine different heterotrophic bacteria were living symbiotically
with B. braunii. These bacteria were classified into four distinct phyla, Actinobacteria, α-proteobacteria, β-proteobacteria
and γ-proteobacteria. Five isolated bacteria particularly Rhizobium sp. demonstrated synergistic relationship with B.
braunii. The growth pattern of B. braunii with or without bacteria was not significantly different for 25 days. However, B.
braunii culture with the inoculation of Rhizobium sp. showed significantly higher (p <0.05) biomass compared to the other
bacterial inoculation. This study illustrated that the symbiotic bacteria could be utilized as inoculants for microalga culture
to optimize the growth and biomass production of B. braunii.