AP Biology ® investigAtive lABs: An Inquiry-Based Approach ( )2 o − e x2 = ∑ e Teacher Manual AP Biology Investigative Labs: An Inquiry-Based Approach The College Board New York, NY Teacher Manual ■ ABouT The College BoArd The College Board is a mission-driven not-for-profit organization that connects students to college success and opportunity. Founded in 1900, the College Board was created to expand access to higher education. Today, the membership association is made up of over 6,000 of the world’s leading educational institutions and is dedicated to promoting excellence and equity in education. Each year, the College Board helps more than seven million students prepare for a successful transition to college through programs and services in college readiness and college success—including the SAT® and the Advanced Placement Program®. The organization also serves the education community through research and advocacy on behalf of students, educators and schools. For further information, visit www.collegeboard.org. ■ AP® equity and Access Policy The College Board strongly encourages educators to make equitable access a guiding principle for their AP programs by giving all willing and academically prepared students the opportunity to participate in AP. We encourage the elimination of barriers that restrict access to AP for students from ethnic, racial and socioeconomic groups that have been traditionally underserved. Schools should make every effort to ensure their AP classes reflect the diversity of their student population. The College Board also believes that all students should have access to academically challenging course work before they enroll in AP classes, which can prepare them for AP success. It is only through a commitment to equitable preparation and access that true equity and excellence can be achieved. The College Board acknowledges all the third party content that has been included in these materials and respects the Intellectual Property rights of others. If we have incorrectly attributed a source or overlooked a publisher, please contact us. Page S121: Reprinted with permission from Micklos and Freyer, DNA Science 2e, © 2003 Cold Spring Harbor Laboratory Press. Page A26: Chart from “The Inquiry Matrix” by Julie Grady, from The Science Teacher, November 2010. ©National Science Teachers Association. Used by permission of NSTA and the author. FAST PLANTS® is a registered trademark of Wisconsin Alumni Research Foundation. Editorial inquiries concerning this book should be directed to The College Board, 45 Columbus Avenue, New York, New York 10023-6992. Second Printing © 2012 The College Board. College Board, Advanced Placement Program, AP, AP Central, and the acorn logo are registered trademarks of the College Board. All other products and services may be trademarks of their respective owners. (Visit the College Board on the Web: www.collegeboard.org.) 2 3 4 5 Dedication On January 4, 2011, after a long, dignified, and courageous battle with pancreatic cancer, the AP Biology community lost a great educator, mentor, and friend, Kim Foglia. Her dedication to helping teachers and students and sharing activities and labs was unparalleled. She was a major supporter of the redesign of the AP Biology course and made significant contributions to the new lab manual. In honor of her vision, dedication, authorship, and contributions, the College Board would like to dedicate AP Biology Investigative Labs: An Inquiry-Based Approach to Kim Foglia. Dedication iii This page is intentionally left blank. Contents Acknowledgments.....................................................................................................................vii ■ For the Teacher Preface..........................................................................................................................................ix Introduction...............................................................................................................................T1 Chapter 1:.How.to.Use.This.Lab.Manual..............................................................................T3 Chapter 2:.The.Labs.at.a.Glance...........................................................................................T11 Chapter 3:.Creating.Student-Directed,.Inquiry-Based.Lab.Investigations.....................T17 Chapter 4:.Managing.the.Investigative.Experience............................................................T23 Chapter 5:.Teaching.Quantitative.Skills.in.AP®.Biology...................................................T27 Chapter 6:.Written,.Verbal,.and.Graphic.Communication...............................................T39 Chapter 7:.Making.AP.Biology.Inclusive.for.All.Learners................................................T43 ■ For the Student Introduction................................................................................................................................S1. Chapter.1:.What.Is.Inquiry?......................................................................................................S3. Chapter.2:.Written,.Verbal,.and.Graphic.Information..........................................................S5. Chapter.3:.Quantitative.Reasoning.in.AP.Biology................................................................S9 ■ Big Idea 1: Evolution Introduction.............................................................................................................................T47 Investigation 1:.Artificial.Selection,.Teacher.Version.......................................................T49 Investigation 1:.Artificial.Selection,.Student.Version........................................................S17 Investigation 2:.Mathematical.Modeling:.Hardy-Weinberg,.Teacher.Version..............T59 Investigation 2:.Mathematical.Modeling:.Hardy-Weinberg,.Student.Version...............S25 Investigation 3:.Comparing.DNA.Sequences.to.Understand.Evolutionary.Relationships. with.BLAST,.Teacher.Version................................................................................................T69 Investigation 3:.Comparing.DNA.Sequences.to.Understand.Evolutionary.Relationships. with.BLAST,.Student.Version................................................................................................S41 ■ Big Idea 2: Cellular Processes: Energy and Communication Introduction.............................................................................................................................T79 Investigation 4:.Diffusion.and.Osmosis,.Teacher.Version................................................T81 Investigation 4:.Diffusion.and.Osmosis,.Student.Version................................................S51 Contents v Investigation 5:.Photosynthesis,.Teacher.Version..............................................................T95 Investigation 5:.Photosynthesis,.Student.Version..............................................................S61 Investigation 6:.Cellular.Respiration,.Teacher.Version...................................................T107 Investigation 6:.Cellular.Respiration,.Student.Version.....................................................S71 ■ Big Idea 3: Genetics and Information Transfer Introduction...........................................................................................................................T121 Investigation 7:.Cell.Division:.Mitosis.and.Meiosis,.Teacher.Version..........................T123 Investigation 7:.Cell.Division:.Mitosis.and.Meiosis,.Student.Version............................S83 Investigation 8:.Biotechnology:.Bacterial.Transformation,.Teacher.Version..............T143 Investigation 8:.Biotechnology:.Bacterial.Transformation,.Student.Version.................S97 Investigation 9:.Biotechnology:.Restriction.Enzyme.Analysis.of.DNA,. Teacher.Version.....................................................................................................................T161 Investigation 9:.Biotechnology:.Restriction.Enzyme.Analysis.of.DNA,. Student.Version......................................................................................................................S111 ■ Big Idea 4: Interactions Introduction...........................................................................................................................T175 Investigation 10:.Energy.Dynamics,.Teacher.Version....................................................T177 Investigation 10:.Energy.Dynamics,.Student.Version.....................................................S125 Investigation 11:.Transpiration,.Teacher.Version............................................................T189 Investigation 11:.Transpiration,.Student.Version............................................................S135 Investigation 12:.Fruit.Fly.Behavior,.Teacher.Version....................................................T203 Investigation 12:.Fruit.Fly.Behavior,.Student.Version....................................................S145 Investigation 13:.Enzyme.Activity,.Teacher.Version.......................................................T215 Investigation 13:.Enzyme.Activity,.Student.Version.......................................................S153 Appendix A:.AP.Biology.Equations.and.Formulas..............................................................A1 Appendix B:.Constructing.Line.Graphs................................................................................A3 Appendix C:.AP.Biology.Concepts.at.a.Glance.................................................................A19 Appendix D:.Matrix.for.Assessing.and.Planning.Scientific.Inquiry.(MAPSI)..............A25 vi Contents Acknowledgments The College Board would like to acknowledge the following individuals and committees for their commitment and dedication toward the completion of this project: ■ Lab authors and Major Contributors • Stacy Baker, Convent of the Sacred Heart, New York, NY • Spencer Benson, University of Maryland, College Park, MD • Elizabeth Cowles, Eastern Connecticut State University, Willimantic, CT • Arthur Eisenkraft, University of Massachusetts, Boston, MA • Kim Foglia, Division Avenue Senior High School, Levittown, New York • A. Daniel Johnson, Wake Forest University, Winston-Salem, NC • John Jungck, Beloit College, Beloit, WI • John Jack Kay, Iolani High School, Honolulu, HI • Sue Offner, Lexington High School, Lexington, MA • Peggy O’Neill Skinner, The Bush School, Seattle, WA • Sharon Radford, The Paideia School, Atlanta, GA • Gordon Uno, University of Oklahoma, Norman, OK • Brad Williamson, University of Kansas, Lawrence, KS • Julianne Zedalis, The Bishop’s School, La Jolla, CA ■ aP Lab ManuaL Vision teaM In 2010, the College Board convened a group of subject matter and laboratory investigation experts to provide a model of excellence for what the investigative labs should be in AP science courses. These individuals worked diligently to create a vision for exemplary AP science labs that would serve to assist teachers in facilitating inquiry- based and student-directed investigative work. This vision also serves as the input for professional development and resource materials that will support the new course and lab investigations. • Arthur Eisenkraft, University of Massachusetts, Boston, MA • Eugenia Etkina, Rutgers University, Piscataway, NJ • Nivedita Ganguly, University of Tennessee, Knoxville, TN • Dolores Gende, Parish Episcopal School, Dallas, TX • Peggy O’Neill Skinner, The Bush School, Seattle, WA • Susan Singer, Carleton College, Northfield, MN • Angelica Stacy, University of California, Berkeley, CA • Peter Strom, Rutgers University, New Brunswick, NJ Acknowledgments vii • Gordon Uno, University of Oklahoma, Norman, OK • Dave Yaron, Carnegie Mellon University, Pittsburgh, PA ■ other Contributors • AP Biology Commission • AP Biology Consultants • AP Biology Curriculum Development and Assessment Committee • AP Biology Development Committee • AP Biology Focus Group Participants • AP Biology Lab Manual Reviewers • AP Biology Lab Pilot Teachers • AP Biology Readers • Authors and Contributors of the 1990 AP Biology Lab Manual (and subsequent editions) • BioPhase Committee • College Board Science Academic Advisory Committee viii Acknowledgments Preface ■ The AP® Biology CurriCulum FrAmework The revised Advanced Placement® Biology course shifts from a traditional teacher- directed “content coverage” model of instruction to one that focuses on helping students gain enduring understandings of biological concepts and the scientific evidence that supports them. This approach enables students to spend more time understanding biological concepts while developing reasoning skills essential to the science practices used throughout their study of biology. A practice is a way to coordinate knowledge and skills in order to accomplish a goal or task. The science practices, as noted in the AP Biology Curriculum Framework, enable students to establish lines of evidence, and use them to develop and refine testable explanations and predictions of natural phenomena. Because content, inquiry, and reasoning are equally important in AP Biology, each learning objective combines content with inquiry and reasoning skills described in the science practices. The key concepts and related content that define the revised AP Biology course and exam are organized around four underlying principles called the big ideas, which are as follows: Big Idea 1: The process of evolution drives the diversity and unity of life. Big Idea 2: Biological systems utilize free energy and molecular building blocks to grow, to reproduce, and to maintain dynamic homeostasis. Big Idea 3: Living systems store, retrieve, transmit, and respond to information essential to life processes. Big Idea 4: Biological systems interact, and these systems and their interactions possess complex properties. These four big ideas will be referred to as evolution, cellular processes: energy and communication, genetics and information transfer, and interactions, respectively, for the sake of brevity. The big ideas encompass the core scientific principles, theories, and processes governing organisms and biological systems. For each big idea, enduring understandings incorporate the core concepts that students should retain from the learning experience. A more student-directed, inquiry-based lab experience supports the AP Biology course revision and curricular requirements by providing opportunities for students to design plans for experiments, data collection, application of mathematical routines, and refinement of testable explanations and predictions. Such a lab experience reinforces the revised curriculum’s focus on quantitative skills. The suite of laboratory investigations within the new lab manual supports the recommendation by the National Science Foundation (NSF) that science teachers build into their curriculum opportunities for students to develop skills in communication, teamwork, critical thinking, and commitment to lifelong learning (Waterman 2008, NSF 1996). These labs will allow teachers to develop and use investigations they design based on their own experiences as an investigator and teacher. The manual’s engaging, inquiry- Preface ix
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