Antiviral Methods and Protocols (Methods in Molecular Medicine)

873 Pages·1999·3.55 MB·English

by  Derek Kinchington

Antiviral Methods and Protocols Methods in
title:
Molecular Medicine ; 24
author: Kinchington, Derek.
publisher: Humana Press
isbn10 | asin: 0896035611
print isbn13: 9780896035614
ebook isbn13: 9780585235684
language: English
Antiviral agents--Laboratory manuals,
Hepatitis, Viral, Human--drug therapy,
subject Antiviral Agents--therapeutic use, DNA
Virus Infections--drug therapy, RNA Virus
Infections--drug therapy.
publication date: 2000
lcc: RM411.A5755 2000eb
616.9/25061
616.9/25061
ddc:
Antiviral agents--Laboratory manuals,
Hepatitis, Viral, Human--drug therapy,
subject: Antiviral Agents--therapeutic use, DNA
Virus Infections--drug therapy, RNA Virus
Infections--drug therapy.
Page i
Antiviral Methods and Protocols
Page ii
METHODS IN MOLECULAR MEDICINE
John M. Walker, S E
ERIES DITOR
42. Vaccine Adjuvants: Preparation Methods and Research
Protocols, edited by Derek T. O'Hagan, 2000
41. Celiac Disease: Methods and Protocols, edited by Michael N.
Marsh, 2000
40. Diagnostic and Therapeutic Antibodies, edited by Andrew J. T.
George and Catherine E. Urch, 2000
39. Ovarian Cancer: Methods and Protocols, edited by John M. S.
Bartlett, 2000
38. Aging Methods and Protocols, edited by Yvonne A. Barnett and
Christopher P. Barnett, 2000
37. Electrically Mediated Delivery of Molecules to Cells, edited by
Mark J. Jaroszeski, Richard Heller, and Richard Gilbert, 2000
36. Septic Shock Methods and Protocols, edited by Thomas J. Evans,
2000
35. Gene Therapy of Cancer: Methods and Protocols, edited by
Wolfgang Walther and Ulrike Stein, 2000
34. Rotavirus Methods and Protocols, edited by James Gray and
Ulrich Desselberger, 2000
33. Cytomegalovirus Protocols, edited by John Sinclair, 2000
32. Alzheimer's Disease: Methods and Protocols, edited by Nigel M.
Hooper, 1999
31. Hemostasis and Thrombosis Protocols: Methods in Molecular
Medicine, edited by David J. Perry and K. John Pasi, 1999
30. Vascular Disease: Molecular Biology and Gene Therapy
Protocols, edited by Andrew H. Baker, 1999
29. DNA Vaccines: Methods and Protocols, edited by Douglas B.
Lowrie and Robert Whalen, 1999
28. Cytotoxic Drug Resistance Mechanisms, edited by Robert Brown
and Uta Böger-Brown, 1999
27. Clinical Applications of Capillary Electrophoresis, edited by
Stephen M. Palfrey, 1999
26. Quantitative PCR Protocols, edited by Bernd Kochanowski and
Udo Reischl, 1999
25. Drug Targeting, edited by G. E. Francis and Cristina Delgado,
1999
24. Antiviral Methods and Protocols, edited by Derek Kinchington
and Raymond F. Schinazi, 2000
23. Peptidomimetics Protocols, edited by Wieslaw M. Kazmierski,
1999
22. Neurodegeneration Methods and Protocols, edited by Jean Harry
and Hugh A. Tilson, 1999
21. Adenovirus Methods and Protocols, edited by William S. M. Wold,
1998
20. Sexually Transmitted Diseases: Methods and Protocols, edited by
Rosanna Peeling and P. Frederick Sparling, 1999
19. Hepatitis C Protocols, edited by Johnson Yiu-Nam Lau, 1998
18. Tissue Engineering, edited by Jeffrey R. Morgan and Martin L.
Yarmush, 1999
17. HIV Protocols, edited by Nelson Michael and Jerome H. Kim,
1999
16. Clinical Applications of PCR, edited by Y. M. Dennis Lo, 1998
15. Molecular Bacteriology: Protocols and Clinical Applications,
edited by Neil Woodford and Alan Johnson, 1998
14. Tumor Marker Protocols, edited by Margaret Hanausek and
Zbigniew Walaszek, 1998
13. Molecular Diagnosis of Infectious Diseases, edited by Udo
Reischl, 1998
12. Diagnostic Virology Protocols, edited by John R. Stephenson and
Alan Warnes, 1998
11. Therapeutic Application of Ribozymes, edited by Kevin J.
Scanlon, 1998
10. Herpes Simplex Virus Protocols, edited by S. Moira Brown and
Alasdair MacLean, 1998
9. Lectin Methods and Protocols, edited by Jonathan M. Rhodes and
Jeremy D. Milton, 1998
8. Helicobacter pylori Protocols, edited by Christopher L. Clayton
and Harry L. T. Mobley, 1997
7. Gene Therapy Protocols, edited by Paul D. Robbins, 1997
6. Molecular Diagnosis of Cancer, edited by Finbarr Cotter, 1996
5. Molecular Diagnosis of Genetic Diseases, edited by Rob Elles,
1996
Page iii
METHODS IN MOLECULAR MEDICINE
Antiviral Methods and Protocols
Edited by
Derek Kinchington
St. Bartholomew's and the Royal London School of Medicine
and Dentistry, London, UK
and
Raymond F. Schinazi
Emory University, Decatur, GA
Humana Press
Totowa, New Jersey
© 2000 Humana Press Inc.
999 Riverview Drive, Suite 208
Totowa, New Jersey 07512
All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or
transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise without written permission from the Publisher. Methods in Molecular
Medicine is a trademark of The Humana Press Inc.
All authored papers, comments, opinions, conclusions, or recommendations are those of the
author(s), and do not necessarily reflect the views of the publisher.
This publication is printed on acid-free paper.
ANSI Z39.48-1984 (American Standards Institute)
Permanence of Paper for Printed Library Materials.
Cover illustration: Figure 1 from Chapter 11, "Antiviral Screening Assays for Human
Herpesviruses 6 and 7," by Jodi B. Black and Philip E. Pellett.
Cover design by Patricia F. Cleary.
For additional copies, pricing for bulk purchases, and/or information about other Humana
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Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1
Library of Congress Cataloging in Publication Data
Main entry under title:
Methods in molecular medicine.
Antiviral methods and protocols/edited by Derek Kinchington and Raymond F. Schinazi.
p. cm.(methods in molecular medicine; vol. 24)
Includes index.
ISBN 0-89603-561-1 (alk. paper)
1. Antiviral agentsLaboratory manuals. I. Kinchington, Derek. II. Schinazi, Raymond F. III.
Series: Methods in molecular medicine; 24.
[DNLM: 1. Hepatitis, Viral, Humandrug therapy. 2. Antiviral Agentstherapeutic use. 3. DNA
Virus Infectionsdrug therapy. 4. RNA Virus Infectionsdrug therapy. WC 536 A633 1999]
RM411.A575 2000
616.9'25061dc21
DNLM/DLC
for Library of Congress 99-25099
CIP
Page v
PREFACE
This latest addition to the Methods in Molecular Medicine series,
Antiviral Methods and Protocols, is opportune because there is an
increasing interest in discovering compounds that are effective against
both chronic and acute viral infections. A number of the methods
described in the volume are unpublished and their inclusion indicates
the speed at which this field is moving. This volume is not a review
but each chapter contains methods validated by the experts who have
spent time in developing the protocols.
The hallmark of this series is the comprehensive way in which the
methods are described, which includes a list of all the reagents needed
for each protocol. Of importance is the section on tips and pitfalls that
the authors have discovered while developing their protocols. The
manual itself is designed to be used by researchers in universities and
industry who are familiar with a range of biological techniques but
who want to set up quickly a novel assay system. We encourage a
dialog between readers and authors, which may also result in useful
collaborations.
The first chapter gives an overview of the change in methodologies
that have been used for discovering active compounds. Safety
considerations have also been reviewed because there are some
significant differences in containment requirements between the
United States and the United Kingdom, for example. Routine quality
testing of cell cultures has also been highlighted because there are
many stories of results being invalidated when target cells have been
contaminated by microorganisms, which, even at low levels, may
significantly alter antiviral endpoints.
For each virus group the chapters that describe the assays start with