Table Of Content

AUG BLDG 10, 10CENTER DR. 8ETHESDA, MD 20892-1150 NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES ANNUAL REPORTS DIVISION OF INTRAMURAL RESEARCH October 1, 1990 to September 30, 1991 ?/ TABLE OF CONTENTS PREFACE Dr. Phillip Gorden, Director I DIVISION OF INTRAMURAL RESEARCH Dr. Allen Spiegel, Director Dr. Edward Steers, Deputy Director PROJECT REPORTS MATHEMATICAL RESEARCH BRANCH Summary 1 Mathematical formulations and analysis relevant to experimental neurophysiology 9 Mathematical description of substrate transport in capillary-tissue structures 10 Mathematical description of cellular neuroelectric signal transmission 11 Electrical and chemical oscillations in coupled eel1 systems 12 LABORATORY OF CELLULAR AND DEVELOPMENT BIOLOGY Summary 13 Protein nucleic acid interactions: chromatin structure and function 22 Study of ribonuclease and its inhibitor from bacillus amyloliquefaciens 23 Studies of folic acid (dihydrofolate reductase) 24 Synthesis and transport of lipoprotein and hepatic lipases in tissues and cells 25 Ultrastructural immunocytochemistry of lipid metabolism in cultured cells and tissues 26 Large-scale processing of biological material 27 Regulation of developmental gene expression 28 Regulation of adipocyte metabolism 29 Control of gene expression in early mammalian development 30 Chromatin structure in regulation of mammalian gene expression 31 § II LABORATORY OF BIOCHEMISTRY AND METABOLISM Summary 32 The role of the carbohydrate moiety of glycoprotein in cellular activity 39 Enzymatic basis of detoxication 40 Polysaccharides in morphogenesis 41 Thermodynamic and kinetic studies of protein structure and enzymic mechanisms 42 The role of the nuclear envelope in intracellular protein sorting 43 Tissue specific and hormone regulated gene expression 44 Inherited Disorders of Lysosomal Function 45 Electrochemical ion gradients as a mechanism of cellular message transmission 46 Cell regulation by Hormones, Growth Factors, Autoantibodies, and Oncogenes 47 Endocytosis, secretion and compartmentalization in mutant CHO eelIs 48 The role of intracellular traffic in HIV infection 49 Cell specific activity of elements within the HIV- LTR 50 Direct measurement of forces between membranes or macromolecules 51 Physics of ionic channels and other proteins with aqueous cavities 52 Structure and physical properties of DNA and DNA - protein complexes 53 Histamine release on a hydration of Granule matrices 54 Cell-cell fusion due to influenza hemagglutinin 55 LABORATORY OF CHEMISTRY Summary 56 Reactions and immunochemistry of carbohydrates 57 Analogues of thyrotropin - releasing hormone 58 Stereopopulation control in drug delivery and enzyme simulation 59 Chemistry of bioimidazoles 60 Halogenated biogenic amines in biochemistry and pharmacology 61 Significance of ligand tautomerism in biorecognition 62 Functionalized cogeners of bioactive compounds 63 Prosthetic groups for radiolabeling of functionalized drugs and peptides 64 Development of drugs acting at adenosine receptors 65 Aldose reductase inhibitors 66 Novel amino acids for conformational and stereochemical constraints in peptides 67 Florinated analogue of bioactive peptides 68