Annual report : National Institute of Diabetes and Digestive and Kidney Diseases

410 Pages·1993·7.7 MB·English

by  National Institute of Diabetes and Digestive and Kidney Diseases (U.S.)

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NATIONAL INSTITUTE OF
DIABETES AND DIGESTIVE
AND KIDNEY DISEASES
ANNUAL REPORTS
DIVISION OF INTRAMURAL
RESEARCH
October 1, 1992 to September 30, 1993
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MAR 9
BETHFSDA. MO 20P92
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TABLE OF CONTENTS
PREFACE
Dr. Phillip Gorden, Director, NIDDK
DIVISION OF INTRAMURAL RESEARCH
Dr. Allen M. Spiegel, Director
Dr. Edward Steers, Deputy Director
PROJECT REPORTS
MATHEMATICAL RESEARCH BRANCH
Summary 1
Mathematical formulations and analysis relevant to
experimental neurophysiology 10
Mathematical description of substrate transport in
capillary-tissue structures 11
Mathematical description of cellular neuroelectric
signal transmission 12
Electrical and chemical oscillations in coupled
cell systems 13
LABORATORY OF CELLULAR AND DEVELOPMENT BIOLOGY
Summary 14
Protein nucleic acid interactions: chromatin
structure and function 32
Study of ribonuclease and its inhibitor from
bacillus amyloliquefaciens 33
Studies of folic acid (dihydrofolate reductase)
and Vitamin A (Beta-Carotene) 34
Synthesis and transport of lipoprotein and hepatic
lipases in tissues and cells 35
Ultrastructural immunocytochemistry of lipid
metabolism in cells and tissue 35
Large-scale production and purification of
compounds with Biological Activity 37
Regulation of developmental gene expression 38
Regulation of Adiocyte Metabolism 39
Control of gene expression in mammalian
development 40
Chromatin structure in regulation of mammalian gene
expression 41
Mechanism of Polyprotein Processing in
Retroviruses 42

LABORATORY OF BIOCHEMISTRY AND METABOLISM
Summary 43
The role of the carbohydrate moiety of glycoproteins
in cellular activity 51
Enzymatic basis of detoxication 52
Polysaccharides in morphogenesis 53
Thermodynamic and kinetic studies of protein
structure and enzymic mechanisms 54
The role of the nuclear envelope in intracellular
protein sorting 55
Tissue specific and hormone regulated gene
expression 56
Inherited Disorders of Lysosomal Function 57
Electrochemical Ion Gradients as a mechanism
of Cellular Message Transmission 58
Cell Regulation by Hormones, Growth Factors,
Autoantibodies, and Oncogenes 59
Endocytosis, Secretion and Compartmentalization
in Mmutant CHO Cells 60
The Role of Intracellular Traffic in HIV
-
Infection 61
Direct Measurement of Forces between Membranes
or Macromolecules 62
Physics of Ionic Channels and other Proteins
with Aqueous Cavities 63
Structure and Physical Properties of DNA and
DNA-Protein Complexes 64
LABORATORY OF CELL BIOLOGY AND GENETICS
Summary 65
Mechanisms of hormone and transmitter secretion 82
Vitamin C: Biochemisty, Molecular Biology and
Human Requirements 83
LABORATORY OF BIOCHEMICAL PHARMACOLOGY
Summary 84
Biochemistry of sulfur-containing compounds 95
The permeability barriers of gram-negatives and
mycobacteria to antibiotics and chemotherapeutics .... 96
Mammalian transposons 97
Bacteriophage T4 gene expression 98
Chemistry and Function of Microtubules 99
Structure and Function of the Tryptophan Synthase
Multienzyme Complex 100
Noncovalent intermolecular interactions
in Biochemistry 101
Enzymatic Mechanisms of DNA Replication:
The Bacteriophage T4 System 102