Animal Cell Technology: From Vaccines to Genetic Medicine

774 Pages·1997·31.441 MB·English

by  C. Weissmann (auth.), Manuel J. T. Carrondo, Bryan Griffiths, José L. P. Moreira (eds.)

ANIMAL CELL TECHNOLOGY
Animal Cell
Technology
From Vaccines to Genetic Medicine
Edited by
Manuel J. T. Carrondo
/BET,
Oeiras, Portugal
Bryan Griffiths
CAMR,
Porton Down, Wiltshire, U.K.
and
Jose L. P. Moreira
/BET,
Oeiras, Portugal
SPRINGER-SCIENCE+ BUSINESS MEDIA, B.V.
A C.I.P. Catalogue record for this book is available from the Library of Congress
ISBN 978-94-010-6273-2 ISBN 978-94-011-5404-8 (eBook)
DOI 10.1007/978-94-011-5404-8
Printed on acid-free paper
Ali Rights Reserved
© 1997 Springer Science+Business Media Dordrecht
Originally published by Kluwer Academic Publishers in 1997
So:ftcover reprint ofthe hardcover 1st edition 1997
No part of the material protected by this copyright notice may be reproduced or
utilized in any form or by any means, electronic or mechanical,
including photocopying, recording or by any information storage and
retrieval system, without written permission from the copyright owner.
Table of Contents
Sponsors & Trade Exhibitors xvii
KEYNOTE LECTURES
Molecular Biology of Prion Diseases
Weissmann, C. 3
Applications of Animal Cell Technology to Drug Discovery and
Pharmacology
Schleuning, W.-D. 7
Biogenesis of Secretory Proteins and Cell Surface Receptors in Mammalian
Cells: Posttranslational Modifications and Protein Folding within the Rough
Endoplasmic Reticulum
Lodish, H.F. 15
SESSION I -Cells and Vectors for Genetic Medicine
Polymer Encapsulated Xenogeneic Cell Lines: a Novel Approach for Gene
Therapy
Aebischer, P. 29
Gene Therapy and Genetic Vaccination: Two Hallmarks of Genetic Medicine
Dalemans, W. 33
PCR Detection of Murine Retroviruses and Characterization of a New
Xenotropic Retrovirus from Myeloma Cells
Wirth, M.; Heinemeyer, T.; Schumacher, L.; Klingenhoff, A.; Hansen, W.;
Suemeghy, Z.; Huber, G.; Schlapfer, B.S.; Wirth, D.; Hauser, H. 37
Toward a DNA Vaccine against Lyssaviruses
Bahloul, C.; Fouque, F.; Tordo, N.; Jacob, Y.; Perrin, P. 43
Principles of a Scaleable Transient Transfection and Expression Technology
for Mammalian Cells
Jordan, M.; Kohne, C.; Wurm, F.M. 47
Neurological Therapy - Adenovirus Mediated Gene Therapy in Cells of the
Central Nervous System
Sivasubramaniam, S.D.; Fooks, A.R.; Lee, J.; Stacey, G.; Jennings, A.D. 51
Bovine Polyoma Virus Detection by Routine PCR Screening
Proffitt, J.; Jarvis, K.; Martin, C. 57
vi
SESSION II- Animal Cells as Tools for Discovery and Testing
Mammalian Cells in High Throughput Screening
Janzen, W. 65
Metabolism and Toxicological Studies in Immortalised Rat Hepatocyte Cell
Lines
MacDonald, C.; Reid, F.; Anderson, K.; Yin, L.; Hill, E.; Kelly, H.; Grant, M.H. 73
Cell Based Assays for Drug Screening
Keenan, E.; Kruger, H.; Sambi, B.; McLean, J.S. 79
Development of Drug Metabolism Simulator Using Three-Dimensional
Culture of Hepatocytes of Rats and Dogs
Funatsu, K.; Nakazawa, K.; Mizumoto, H.; Takenaka, K.; Matsushita, T. 85
Immobilization of Primary Brain Cells in Porous Microcarriers for on Line
NMR Spectroscopy
Alves, P.M.; Carrondo, M.J.T.; Santos, H. 91
Application of Primary Hepatocyte Sandwich Cultures for Predictive Studies
of Drug Metabolism and Precarcinogen Activation
Bader, A; Fahrig, R.; Steinkamp, A; Fruhauf, N.; Crome, 0.; Zech, K.;
Esselmann, H.; Gonschior, A K.; Sewing, K. Fr. 99
Increased Cytotoxic Sensibility of Cultured Fathead Minnow Fish Cells by
Simultaneous Treatment with Sodium Dodecyl Sulfate and Buthionine
Sulfoximine
Dierickx, P.J.; Govers, L. 103
Immortalization and Characterization of Porcine Brain Microcapillary
Endothelial Cells
Teifel, M.; Friedl, P. 109
Three-Dimensional Cell Culture Systems Stabilise the Differentiation of
Hepatocyte Cell Lines
Gon<;alves, L.M.D.; Lopez, T.; MacDonald, C.; Grant, M.H.; Carrondo, M.J.T. 115
In-Vitro Toxicological Effects of Pluronic F -68
Handa-Corrigan, A; Weeratunge, N.P.; Braybrook, J.H.; Mackay, G.A;
Chescoe, D. 121
Genotoxicity of Bacterial Ribonuclease in Mammalian Cells after its
Application In Vitro and In Vivo.
Ivanchenko, O.B.; Karamova, N.S.; Ilinskaya, O.N. 127
Use of Cell-Based Assays for In Vitro Studies of Drugs
Riva, S.; Brunati, C.; Pollini, W.; Quarta, C.; Nolli, M.L. 133
vii
Evaluation of Cytotoxicity with Fish Cell Lines: a Case Study for
a.-Oiigothiophenes (1-5 Rings)
Lopes, A.; Gon~alves, L.M.D.; Carrondo, M.J.T. 139
The Effect of Cell Culture System on the Phenotypic Stability in Primary
Hepatocyte Cells
Lopez, T.; Carrondo, M.J.T.; Gon~alves, L.M.D. 145
SESSION Ill - Animal Cell Vaccines - Present and Future
New Emerging Viruses: Potential Risk of Contamination of Biological
Products
Saluzzo, J.F. 153
Development and Production of New Live Measles Vaccine Based on Human
Embryo Lung Diploid Cell Culture L-68
Nechaeva, E.A.; Kolokoltsova, T.D.; Jurchenko, N.D.; Kits, LV.; Jilina, N.;
Sandakhchiev, L.S. 159
Development of Influenza Subunit Vaccine Produced Using Mammalian Cell
Culture Technology
Brands, R.; van Scharrenburg, G.J.M.; Palache, A.M. 165
Monitoring and Control of Large-Scale Vaccine Production via Dynamic
Oxygen Uptake Rate Measurement
Preissmann, A.; Werner, R.G.; Noe, W. 169
TM
Reactor Development for the Hepatitis A Vaccine VAQTA
Aunins, J.; Bibila, T.A.; Gatchalian, S.; Hunt, G. R.; Junker, B.; Lewis, J.A.;
Licari, P.; Ramasubramanyan, K.; Ranucci, C.S.; Seamans, T.C.; Seifert, D.B.;
Zhou, W.; Waterbury, J.; Buckland, B.C. 175
Production of Live Attenuated Varicella-Zoster Virus in Human Embryonic
Lung Cells Using Microcarrier
Chun, B. H.; Woo, G. J.; Cheung, H.-S.; Moon, H.M. 185
Production of Lyssav irus Proteins in the Baculovirus Expression System and
their Use as Potential Vaccine
Drings, A.; Chambert, B.; Jallet, C.; Tordo, N.; Perrin, P.; Jacob, Y. 191
Development of Production Technology of Live Measles Vaccine for Peroral
Administration
Nechaeva, E.A.; Varaksin, N.A.; Ryabicheva, T.A.; Kolokoltsova, T.D.; Ignatyev,
I.M.; Agafonov, A.P.; Kolokoltsov, A.A.; Yurchenko, N.D.; Sandakhchiev, L.S. 197
viii
SESSION IV - Animal Cell Process Engineering
On-Line Monitoring for Consistent and Optimal Production of Biologicals
from Mammalian Cell Cultures
Ozturk, S.S.; Blackie, J.; Wu, P.; Taticek, R.; Konstantinov, K.; Matanguihan, C.;
Tsai, Y.; Yoon, S.; Thrift, J.; Naveh, D. 205
On the Use of Electric Fields to Prevent Membrane Fouling of Process
Integrated Cross Flow Filtration at Hybridoma Cell Cultures
Zumbusch, P.V.; Portner, R.; Brun, B.; Brunner, G.; Miirkl, H. 215
Simplified Screening of Monoclonal Antibodies for Immunoaffinity
Chromatography -A Case Study on Anti-hiL-2-Antibodies
Vogel, J.H.; Randolph, 1.; Kroner, K.-H. 221
Integrative Antibody Purification from Whole Broth by Fluidized Bed
Adsorption
Thommes, J.; Bader, A.; Karau, A.; Kula, M.R. 227
Large-Scale Production of Humanized Monoclonal Antibody Expressed in a
GS-NSO Cell Line
Ray, N.G.; Rivera, R.; Gupta, R.; Mueller, D. 235
2-D PAGE Examination of the Proteins Extracted from High Density
Perfused HeLa Cell Cultures Controlled by an On-Line Capacitance-Based
Biomass Probe
Degouys, V.; Gatot, C.; Sonderhoff, S.; Trampler, F.; Miller, A.O.A. 243
Experimental and Theoretical Analysis of Cell Separations by Ultrasonic
Forces
Woodside, S.M.; Bowen, B.D.; Piret, J.M. 251
Factors Affecting the Production and Quality of IgA Antibodies Active
Against V. Cholerae
Marison, 1.; Stoll, T.S.; Uillau, E.; von Stockar, U. 257
Ceramic Hydroxyapatite: a New Tool for Separation and Analysis of IGA
Monoclonal Antibodies
Lullau, E.; Marison, I.W.; von Stockar, U. 265
"Bionebulisation": an Alternative to Dounce Homogenization for the
Preparation of Mammalian Cells Nuclei in Bulk
Degouys, V.; Montoisy, C.; Wanderpepen, M.P.; Togasaki, R.K.; Surzycki, S.J.;
Segault, V.; Miller, A.O.A. 271
Epitope Determination for Antibodies Raised Against Recombinant Human
Interferon-Gamma
Hooker, A.D.; Green, N.H.; James, D.C.; Strange, P.G.; Baines, A.J.; Bull, A.T.;
Jenkins, N. 277
ix
A New Spinfilter Design
Fraune, E.; Meichsner, S.; Kamal, M.N. 283
On-Line Biomass Monitoring of Immobilized Hybridoma Cells by
Dielectrical Measurements
Noll, T.; Biselli, M.; Wandrey, C. 289
Evaluation of the Proteolytic Activity Present in CHO Cell Culture
Supernatants
Tans, C.; Wattiaux-DeConinck, S.; Gonze, M.M.; Fabry, L. 295
CeDEX: Automated Cell Density Determination
Gudermann, F.; Ziemeck, P.; Lehmann, J. 301
Design and Performance of a Perfusion Based Packed Bed Bioreactor for the
Production of Secreted Protein
Kadouri, A.; Wang, G.; Zhang, W.; Jacklin, C.; Freedman, D. 307
Low-and High-Producing Animal Cell Cultures in Perfused, Packed Beds of
Non-Woven Fabric
Treat, J.C.; Sandelin, K.K.; Beiderman, B.C.; Kirschner, R.J. 313
Temperature -a Factor Influencing Cell Behaviour
Kretzmer, G.; Rossler, B.; Hiiners, M.; Lubben, H.; Reich, T. 319
Direct Capture of Monoclonal Antibodies Using High Capacity Membrane
Ion Exchangers in Pilot Scale
Luetkemeyer, D.; Ameskamp, N.; Tebbe, H.; Bracht, K.; Lehmann, J. 325
Influence of Mass Culture Operating Conditions on the Apoptosis of OKT3
Hybridoma Cells
Pauly, H.; Franck, P.; Nabet, P.; Cherlet, M.; Marc, A. 331
Increased Production of Recombinant hiGFBP-1 in Self-Fused Chinese
Hamster Ovary (CHO) Cells
Dyring, C. 337
New Process for Cell Detachment: Use of Heparin
Merten, 0.-W.; Dante, J.; Noguiez-Hellin, P; Laune, S.; Klatzmann, D.;
Salzmann, J.-L. 343
Development of a High Sensitivity Retrovirus Specific Reverse Transcriptase
Assay
Chang, A.; Wisher, M.; Bird, R.; Ostrove, J.M. 349
Kinetics of Glucose Metabolism and Utilization of Lactate in Mammalian
Cell Cultures
Ozturk, S.S.; Jorjani, P.; Taticek, R.; Lowe, B.; Shackleford, S.; Ladehoff-Guiles,
D.; Thrift, J.; Blackie, J.; Naveh, D. 355
X
"Nutrient-Split" Feeding Strategy for Dialysis Cultures of Immobilized
Hybridoma and Transfectoma Cells
Ludemann, 1.; Portner, R.; Neumann, R.; Mli.rkl, H. 361
Immobilization can Improve the Culture Stability of Non-Anchorage-
Dependent Animal Cells in Serum-Free Media
Ludemann, 1.; Portner, R.; Schaefer, C.; Schick, K.; Snirnkova, K.; Reher, K.;
Franek, F.; Neumaier, M.; Mli.rkl, H. 367
Influence of Pluronic F -68 on the Ultrafiltration of Cell Culture Supernatants
Schulz, C.; Vogel, J.H.; Scharfenberg, K. 373
Dynamic Membrane Filtration in Mammalian Cell Culture Harvest
Kempken, R.; Rechtsteiner, H.; Schafer, J.; Katz, U.; Dick, 0.; Weidemeier, R.;
Sellick, I. 379
Culture of Human Intestinal Cells in Simulated Microgravity
Latigo, J.R.; Battle, T.P.; Hall, G.; Cook, R.; Freemantle, P.; Stacey, G.N. 385
Removal of Animal Cells and Cell Debris by Continuous Two-Stage
Centrifugation
Tebbe, H.; Gudermann, F.; Luetkemeyer, D.; Lehmann, J. 391
Large Scale Bubble Bed Aeration for Animal Cell Cultures
Trocha, M.; von Rohr, R. 399
Contribution to the Understanding of Retention of Animal Cells in
Spinfilters
Trocha, M.; von Rohr, R.; Siimeghy, Z. 405
Rotating Disk Microfiltration of Cell Culture Broth -Effect of Shear Force
and Cell Stability on Filtration Performance
Vogel, J.H.; Kroner, K.H. 411
Process Control of Antithrombin III Production by Near-Infrared
Spectroscopy
Harthun, S.; Matischak, K.; Friedl, P. 417
Selection of an Immobilization Method for a Perfusion Bioreactor with
Hybridoma Cells
Tint6, A.; Vila, P.; Casas, C.; GOdia, F. 423
On-Line Monitoring of Glutamine and Ammonium in Mammalian-Cell
Cultures
Jorgensen, E.B.; Casablancas, A.; Gonzalez, G.; GOdia, F. 429
Study for the Production of a Recombinant Monoclonal Antibody in Stirred
Tank
Boggiano, T.; Fernandez, A.; Hermida, C.; Villan, J.; Ojto, E.; Hidalgo, Y.; Arias,
M.; Gomez, J. 435