Analytical Biochemistry 3rd ed - D. Holme, H. Peck (1998) WW

501 Pages·1998·13.48 MB·English

THIRD EDITION

7AN NAN Ey ey AN
Analytical Biochemistry
Third Edition.

David J. Holme and Hazel Peck

Pear Education,
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Ati Hosp Compendia

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Foneein Seispoe OS
Contents

Preface ote id etn vii
refuse othe cece eon *
Dna wth tis x
Abc baer a rf test qui ai
1 General princpkes of analytical hachemsn ‘
he selection ul vil ala ay 2
he uy ol da ‘
the acta ul mele »
Spectrosc 28
iertiom a rtition wih ater *
Molec absumpmaery “
‘Absorpiemeter desizm o
‘Molectlustuorescece tohriques 3
‘Atrncspostescopy ech ges %6
‘Micnace resonance spectonceny #8
3 Soparation methods oo
rineiptes of spartion teehlques os
‘Matlngs has nn polar %
‘Mash aad an nis nae rl
‘Method had mn see 16
‘Maths Ried ae ie
4 Mlecwsoaltis! methods
Cotey
Soliceliy
omer
canter

0

"

n

Radlolntopes
Nenu of adoastiity

DDeeton and marurement of radioactivity
Bleshomical uze of aopes

‘ntomated methods of analysis
Discrete anlvers

Flow anys,

Robotics

Amananologieal methods
‘General processes uf thr mmuge sponse
“Antgen-anibedyrewlone
‘Snatcaltenigoes~preciatin euctins
‘Agate tcanignes ~inmumcessy

Enzymes
Tre nate of enzyaee

zyme uy eho

Monitoring tetaigars

Tremtoen of smples tor enya us
Sebsoie assy methods

‘vomit sous

mote enzymes

Carbolyteates
Gear sre an ston

(Chemical mbes abet lis

lenge metas hokey
Sepenition na identical cable meres

{Gente saci art pps
Generates

Steal mie
esctome of specFc sin
Seperain of ann ral mises
‘Armin chalet

Protas
tin ute

Cnet aio of.artigan
Separation of pein

plas
Foe aed
Sime iid
Compl inde
Contents vi

Lipid-proten souctrce 421
Sample perpcuton and tang a4
(Qounitave mebode 405
Separation of Lipid mites cs

AS Nowe uci a
[Nutieic si compesten acd steucure 7
molten ad purction nf ee hls wy
Methods uf alee acd males, 456
etre 565
DNA seercng, 68

Aggendie: nner and clawsicaina a erryaes a

Sete parson ares 29

Ines ef
Preface

1 ERE: Pathe HIATON
‘The teuislogyasociaa th bss ts i rapidly changing al
es nsttyinouuat ane carly hei ntennced. Tor,
{Ga escape inane te not Bas new prin af ui
hut oe ail bows atm tosh ae and mate approach. For
omy ple. dar HPLC sean vay Oo ean tat caine vagus
Fert Sie separation a fe ang seta api, dhe hen
ssi betwstr decisis ehowaragephy astm Ered such
Teainiquct 36 esl chrowatetsphy ane mse apsaonet, pris
trv asa wa LC, fem Pol to ac wr sarc chr
wanoaraplie txlniqace. These "ate the at nations ae worlly
tiierpanuston cite es tim: digs oF aowaion al ste we
Tse dosignod fr ato oe

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rigs theinoostnce of uideetacing te eincpie of hs oehnigu a
“king, Unlesthic sca hey wl easy en be abla, opine
tid csetep nes tins, toblehoot oxstngonseané be cident in he
uals of tie eocts Wh isoreasing imporanes being anebutcd tepals
fSruraiee and laboratory aerediwion 1 20tion co dh. fad engl
‘ire dor staf 1 wake fiom a appeciation of nda pene
Hl analysis nr. We teteove makin aplpgis for gan concen
Jig thos inhi thie edition

"Wc have doled ome soctos tha contains dalled acount af ts
siqus thr ae rarsly encountered 'n modeta Ibotuocie. whe retain c=
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sanding ed make the book more "ase finaly. Too ts of sl st que
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“ule ofthe wits We Rave decided act fo inetde pstogzaph of pat-
{uly nena they ote ollea nol particle sabia aa Te
tls ange ao cpidy. We woah! Uke to thank De St Cad ad Dr
ober Shih or revising tei Copies em sue acide apd ixasogicl
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Princes of the subeer thay wll adhe wnt Fa we range of
att he ae ith ting Fra quaication orn are werk ng ina et
fra: oF pers Yom, The asing list atthe end ofeach Cua anges
dion sr for nadoes wh qui mors dst of spec pis.

David Hote
Heal eck

Apel 1997
Preface

BV IDR srcosn somes
Siace te pubicanon of the Get elikan ww 19K, several specivis books
which over a inge of spec tehzighes 1m ee Beem published
severe aby 1 els appre temp a ical et
fvalgeoler il emg Landaa he fe ein ts Buh
ealy pre use i fo repent, Cathe pelo belive J tis Se
‘al eto remains unchange

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dal Netto deta of spec pasting are ma ed,
Thy exami, DNA frgersnung ire appa, we ne inl es
‘at hook whi ase steps napa he arr al
The eft el: chapter Thee Intl lee tae ally enelesie
I ae th rape refeancl esa Hi 1 be inagprapite f The
Iolo ptr een,

‘We ive eo many asa pnt lene het
tin in ge of abated Inwavic taa such a pom mils,
biossnaaleys,apechonvical, nical iochonisey.aefeclar hays. te
ar vn capstan al canny allege vie aries ee
reinfored Ar itUal pepo wf itn, 4 one Ft lente mange Of
nusee hat ince the analy Gal aepaie of Kichetviay. We ne Bene
‘Elighted that thie setback edition It wen? stills which ill aw sHTage
‘wider coon for sudene wie,

Hart Posie
David Holme
‘Shep Hatin aivrsty

uy 1992
Preface

TO THE FIRST EDITION
The nil sinus for srg his hak ame cat of Acai eperionced
in crnmending gle sie stbo9e for sal en erie i wh
the anslyncal aspece of Hochemésuy were major component AONE
her are oun beaks on alia chemishy in greta an clini homiy
in parenls, rary anit he Biochrical aspects of maleic such as carynah
spy aud immwaalogy wile oes da sot cove the baie siete of the
Jeo The nyesive ay to rg he noe Suk he te while
ansder oe exsetl to the subj af anal ochens.

Inthe ieductory section to cae’ chapter, tow is bit exphnation of
‘he scents bare ofthe ep and this fllewod by adiseusson of he ana-
Iya method which an releval, While titan hat ith. be
buon of reap, tettcal dec dor mic uf the wean eb se
hen, This il tp (bos ender wit an pecicalexperieae a apprevite
fhe sepeimvelyd indie anelyen sie cee same tine giving nce etal
forthe nethed wo Ne descloped in peace. Ts ialnded Ba hoa wil
oer ifort eta a te ele 9 leigh ein
Uterine which ele be appre or v panicle olga poblen
sana be ablote develop lid and reliable uli] eho

‘The tice covered inthis hos falco ee tain genupe, Ameya
techniguot sich a apactonpy, shiomatngaphy. ee. ae play wap
taal in analytic 5 Taina ancy general I
Sewage of ace tchnigne ae expen malt cup a apps me
‘iscasid. Tote exe cheer om enzcs, smibeies and raiswopes, ub-
stances hich it maybe necestary to dete and measure tar which alsa cap
every nea in a vaso af anapticl methods. Hove agai. the hate Bony
is eels he‘ore kesung ieie agian on analytical ober.
vay tre ae fone ebapters wc eplan he hal atime el
ds of analysis of the expr isoups of blseyuly iespentan cements,
samc. cartohydraes, anno sds, proteins avd lips. Whe its apc
hat the range of compounds in this inl section opuld he consgerably
lend hs Ian deliertely evict th arp tl we Conde
fr ohwot particule Heche! napurtince

‘A toe end of ech cape, several books este for Loter vn
on tho aubjet ta cs sogpested three folsoing 200k weil be wable
fr Furbo reading on the opi ef bohemian acids, cabotya
pie na,