An ultrastructural study on the contractile pinacocyte of a freshwater sponge PDF
Preview An ultrastructural study on the contractile pinacocyte of a freshwater sponge
: i98 MEMOIRS OF THE QUEENSLAND MUSEUM AN ULTRASTRUCTURAL STUDY ON THE had many bundles extendingradially from the central CONTRACTILE PINACOCYTE OF A nuclear zone to the peripheral region of the cell, FRESHWATER SPONGE. Memoirs of the whereas these bundles were not observed in the inner QueenslandMuseum 44: 398. 1999;-Contractilecells pinacocytes. Bundles were easily stained using inspongeswerefirstobservedintheosculardiaphragm NBD-phallacidin. Observations of thin sections of%marine species, Microeiona and Tedania (Bagby, showed these bundles are composed of many thin 1 1). They consistedofwell-differentiated myocytes filamentsofabout4-6nm diameter. These bundles ran having myosin and actin filaments, whereas in the straight in thecontracted state, and were distributed in freshwater sponges, there are no reports ofcontractile thebasalregionofthecell.Thinfilamentsinthebundle apparatus or myocytes.The oscular diaphragms and were clearly decorated with anti-actin gold bodywallsoffreshwaterspongesarecontractiblewhen conjugation. SDS-PAGE analysis of the diaphragm stimulated. Stimulation is effective whether it is revealedaprotein band of45kD. Theseresultssupport osmotic, thermal, electric, etc. According to these the idea that thin filamentsofabout 4-6nm indiameter observations on sponges we hypothesise that in pinacocytes are composed of actin molecules. A pinacocytes in the oscular diaphragm and body wall freshwater sponge, Ephydatia fluviatilis, has no must bearthe contractile apparatus, and we suggested myocytes but has contractile pinacocytes with actin G in a previous report that these cells have a network of bundles. Porifeta, freshwater sponges, contractile filamentous bundles. We subsequently attempted to filaments, uitrastrueture, actin, pinacocytes. structurally identify thecontractile filamentsofthecell usingthefollowingmethods: 1)Observationsunderlight Akira Matsuno (email, nuttswiotalife.shimane-u.ae.jp) & microscopy;2)Observationsunderelectronmieroseop\ Masaaki Kuroda. Department of Biological Sciences, 3) SDS-PAGE; 4) NBD-phallacidin staining; 5) Faculty of Life and Environmental Science. Shimutic Anti-actingoldconjugation.Pinacocytesinthesesponges University, Matsite 690, Japan; Yoshiki Masuda, showed a flat and multiangularshape, measuring about Department of Biology, Kawasaki Medical School, 5um in diameter and 0.1urn thick. Pinacocytes in the KurashikiCity-, Okayama 701-01, Japan; 1June 1998. outer layer ofthe oscular diaphragms and body wall
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