alleviation of the effects of mungbean yellow mosaic virus in blackgram through foliar application of PDF
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ALLEVIATION OF THE EFFECTS OF MUNGBEAN YELLOW MOSAIC VIRUS IN BLACKGRAM THROUGH FOLIAR APPLICATION OF SALICYLIC ACID, TRIACONTANOL AND MINERAL NUTRIENTS BY K. BASAVARAJ B.Sc. (Ag.) THESIS SUBMITTED TO THE ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN AGRICULTURE (PLANT PATHOLOGY) CHAIRMAN: Dr. J. KRISHNA PRASADJI DEPARTMENT OF PLANT PATHOLOGY AGRICULTURAL COLLEGE, BAPATLA ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY 2012 CERTIFICATE Mr. K. BASAVARAJ has satisfactorily prosecuted the course of research and that thesis entitled “ALLEVIATION OF THE EFFECTS OF MUNGBEAN YELLOW MOSAIC VIRUS INFECTION IN BLACKGRAM THROUGH FOLIAR APPLICATION OF SALICYLIC ACID, TRIACONTANOL AND MINERAL NUTRIENTS” submitted is the result of original research work and is of sufficiently high standard to warrant its presentation to the examination. I also certify that neither the thesis nor its part thereof has been previously submitted by him for a degree of any University. Date: (J. KRISHNA PRASADJI) Chairman Professor and Head Department of Plant Pathology Agricultural College, Bapatla CERTIFICATE This is to certify that the thesis entitled “ALLEVIATION OF THE EFFECTS OF MUNGBEAN YELLOW MOSAIC VIRUS INFECTION IN BLACKGRAM THROUGH FOLIAR APPLICATION OF SALICYLIC ACID, TRIACONTANOL AND MINERAL NUTRIENTS” submitted in partial fulfilment of the requirements for the degree of ‘Master of Science in Agriculture’ of the Acharya N. G. Ranga Agricultural University, Hyderabad is a record of the bonafide original research work carried out by Mr. K. BASAVARAJ under our guidance and supervision. No part of the thesis has been submitted by the student for any other degree or diploma. The published part and all assistance received during the course of the investigations have been duly acknowledged by the author of the thesis. (J. KRISHNA PRASADJI) Chairman of the Advisory Committee Thesis approved by the student’s advisory committee Chairman Dr. J. KRISHNA PRASADJI Professor and Head Department of Plant Pathology Agricultural College, Bapatla Member Dr. P. ANIL KUMAR Senior Scientist (Plant Pathology) Agricultural Research Station Nellore Member Dr. M. ADINARAYANA Senior Scientist (Plant Pathology) Regional Agricultural Research Station Lam Farm, Guntur Member Dr. G. RAMACHANDRA RAO Senior Scientist (Entomology) DAATT Centre Lam Farm, Guntur Date of final viva-voce: DECLARATION I, K. BASAVARAJ hereby declare that the thesis entitled “ALLEVIATION OF THE EFFECTS OF MUNGBEAN YELLOW MOSAIC VIRUS INFECTION IN BLACKGRAM THROUGH FOLIAR APPLICATION OF SALICYLIC ACID, TRIACONTANOL AND MINERAL NUTRIENTS” submitted to the Acharya N.G. Ranga Agricultural University for the degree of Master of Science in Agriculture is the result of original research work done by me. I also declare that no material contained in the thesis has been published earlier in any manner. Place: Bapatla (K. BASAVARAJ) I. D. No. BAM-10-40 Date: LIST OF TABLES Page S.No Title No 4.1 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV incidence during kharif 2011-12 in field 4.2 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV incidence during kharif 2011-12 in pot culture 4.3 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV incidence during rabi 2011-12 in field 4.4 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV incidence during rabi 2011-12 in pot culture 4.5 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV severity (PDI) during kharif 2011-12 in field 4.6 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV severity (PDI) during kharif 2011-12 in pot culture 4.7 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV severity (PDI) during rabi 2011-12 in field 4.8 Effect of salicylic acid, triacontanol and mineral nutrients on MYMV severity (PDI) during rabi 2011-12 in pot culture 4.9 Effect of salicylic acid, triacontanol and mineral nutrients on root length and shoot length during kharif 2011-12 in field 4.10 Effect of salicylic acid, triacontanol and mineral nutrients on root length and shoot length during rabi 2011-12 in field 4.11 Effect of salicylic acid, triacontanol and mineral nutrients on root length (cm) of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.12 Effect of salicylic acid, triacontanol and mineral nutrients on root length (cm) of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.13 Effect of salicylic acid, triacontanol and mineral nutrients on shoot length (cm) of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.14 Effect of salicylic acid, triacontanol and mineral nutrients on shoot length (cm) of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.15 Effect of salicylic acid, triacontanol and mineral nutrients on number of primary branches per plant and number of pods per plant during kharif 2011-12 in field 4.16 Effect of salicylic acid, triacontanol and mineral nutrients on number of primary branches per plant and number of pods per plant during rabi 2011-12 in field 4.17 Effect of salicylic acid, triacontanol and mineral nutrients on number of primary branches per plant of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.18 Effect of salicylic acid, triacontanol and mineral nutrients on number of primary branches per plant of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.19 Effect of salicylic acid, triacontanol and mineral nutrients on number of pods per plant of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.20 Effect of salicylic acid, triacontanol and mineral nutrients on number of pods per plant of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.21 Effect of salicylic acid, triacontanol and mineral nutrients on number of seeds per pod and seed yield (q/ha) during kharif 2011-12 in field 4.22 Effect of salicylic acid, triacontanol and mineral nutrients on number of seeds per pod and seed yield (q/ha) during rabi 2011-12 in field 4.23 Effect of salicylic acid, triacontanol and mineral nutrients on number of seeds per pod of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.24 Effect of salicylic acid, triacontanol and mineral nutrients on number of seeds per pod of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.25 Effect of salicylic acid, triacontanol and mineral nutrients on seed yield per plant of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.26 Effect of salicylic acid, triacontanol and mineral nutrients on seed yield per plant of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.27 Effect of salicylic acid, triacontanol and mineral nutrients on 100 seed weight (g) and total plant dry matter (g) during kharif 2011-12 in field 4.28 Effect of salicylic acid, triacontanol and mineral nutrients on 100 Seed weight (g) and total plant dry matter (g) during rabi 2011-12 in field 4.29 Effect of salicylic acid, triacontanol and mineral nutrients on 100 seed weight of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.30 Effect of salicylic acid, triacontanol and mineral nutrients on 100 seed weight (g) of MYMV infected and healthy blackgram plants during rabi 2011-12 in pot culture 4.31 Effect of salicylic acid, triacontanol and mineral nutrients on total plant dry matter (g) of MYMV infected and healthy blackgram plants during kharif 2011-12 in pot culture 4.32 Effect of salicylic acid, triacontanol and mineral nutrients total plant dry matter (g) during rabi 2011-12 in pot culture 4.33 Effect of salicylic acid, triacontanol and mineral nutrients on peroxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.34 Effect of salicylic acid, triacontanol and mineral nutrients on peroxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.35 Effect of salicylic acid, triacontanol and mineral nutrients on polyphenol oxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.36 Effect of salicylic acid, triacontanol and mineral nutrients on polyphenol oxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.37 Effect of salicylic acid, triacontanol and mineral nutrients on phenylalanine ammonia lyase activity (η moles of transcinnamic acid/min/g) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.38 Effect of salicylic acid, triacontanol and mineral nutrients on phenylalanine ammonia lyase activity (η moles of transcinnamic acid/min/g) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.39 Effect of salicylic acid, triacontanol and mineral nutrients on total phenols (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.40 Effect of salicylic acid, triacontanol and mineral nutrients on total phenols (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.41 Effect of salicylic acid, triacontanol and mineral nutrients on soluble proteins (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.42 Effect of salicylic acid, triacontanol and mineral nutrients on soluble proteins (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.43 Effect of salicylic acid, triacontanol and mineral nutrients on total chlorophyll (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.44 Effect of salicylic acid, triacontanol and mineral nutrients on total chlorophyll (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.45 Effect of salicylic acid, triacontanol and mineral nutrients on total sugars (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.46 Effect of salicylic acid, triacontanol and mineral nutrients on total sugars (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 LIST OF FIGURES Page S.No Title No 3.1 Lay out of field experiment on alleviation of effects of mungbean yellow mosaic virus in blackgram through foliar application of SA, tria and mineral nutrients 4.1 MYMV incidence (%) in the best treatment (tria) and unsprayed check during kharif 2011-12 field 4.2 MYMV incidence (%) in the best treatment (tria) and unsprayed check during kharif 2011-12 in pot culture 4.3 MYMV incidence (%) in the best treatment (tria) and unsprayed check during rabi 2011-12 in field 4.4 MYMV incidence (%) in the best treatment (tria) and unsprayed check during rabi 2011-12 in pot culture 4.5 MYMV severity (PDI) in the best treatment (tria) and unsprayed check during kharif 2011-12 in field 4.6 MYMV severity (PDI) in the best treatment (tria) and unsprayed check during kharif 2011-12 in pot culture 4.7 MYMV severity (PDI) in the best treatment (tria) and unsprayed check during rabi 2011-12 in field 4.8 MYMV severity (PDI) in the best treatment (tria) and unsprayed check during rabi 2011-12 in pot culture 4.9 Effect of salicylic acid, triacontanol and mineral nutrients on root length and shoot length during kharif 2011-12 in field 4.10 Effect of salicylic acid, triacontanol and mineral nutrients on root length and shoot length during rabi 2011-12 in field 4.11 Effect of salicylic acid, triacontanol and mineral nutrients on number of primary branches per plant, number of pods per plant and number of seeds per pod during kharif 2011-12 in field 4.12 Effect of salicylic acid, triacontanol and mineral nutrients on number of primary branches per plant, number of pods per plant and number of seeds per pod during rabi 2011-12 in field 4.13 Effect of salicylic acid, triacontanol and mineral nutrients on seed yield (q/ha), 100 seed weight (g) and total plant dry matter (g) during kharif 2011-12 in field 4.14 Effect of salicylic acid, triacontanol and mineral nutrients on seed yield (q/ha), 100 Seed weight (g) and total plant dry matter (g) and during rabi 2011-12 in field 4.15 Effect of salicylic acid, triacontanol and mineral nutrients on peroxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.16 Effect of salicylic acid, triacontanol and mineral nutrients on peroxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.17 Effect of salicylic acid, triacontanol and mineral nutrients on polyphenol oxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.18 Effect of salicylic acid, triacontanol and mineral nutrients on polyphenol oxidase activity (∆ Abs/min/g) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.19 Effect of salicylic acid, triacontanol and mineral nutrients on phenylalanine ammonia lyase activity (η moles of transcinnamic acid/min/g) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.20 Effect of salicylic acid, triacontanol and mineral nutrients on phenylalanine ammonia lyase activity (η moles of transcinnamic acid/min/g) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.21 Effect of salicylic acid, triacontanol and mineral nutrients on total phenols (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.22 Effect of salicylic acid, triacontanol and mineral nutrients on total phenols (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.23 Effect of salicylic acid, triacontanol and mineral nutrients on soluble protein (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.24 Effect of salicylic acid, triacontanol and mineral nutrients on soluble protein (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.25 Effect of salicylic acid, triacontanol and mineral nutrients on total chlorophyll (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.26 Effect of salicylic acid, triacontanol and mineral nutrients on total chlorophyll (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12 4.27 Effect of salicylic acid, triacontanol and mineral nutrients on total sugars (mg/100 mg) in MYMV infected and healthy blackgram plants during kharif 2011-12 4.28 Effect of salicylic acid, triacontanol and mineral nutrients on total sugars (mg/100 mg) in MYMV infected and healthy blackgram plants during rabi 2011-12
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