Advances in Immunoassay Technology PDF
Preview Advances in Immunoassay Technology
ADVANCES IN IMMUNOASSAY TECHNOLOGY Edited by Norman H. L. Chiu and Theodore K. Christopoulos Advances in Immunoassay Technology Edited by Norman H. L. Chiu and Theodore K. Christopoulos Published by InTech Janeza Trdine 9, 51000 Rijeka, Croatia Copyright © 2012 InTech All chapters are Open Access distributed under the Creative Commons Attribution 3.0 license, which allows users to download, copy and build upon published articles even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech, authors have the right to republish it, in whole or part, in any publication of which they are the author, and to make other personal use of the work. Any republication, referencing or personal use of the work must explicitly identify the original source. As for readers, this license allows users to download, copy and build upon published chapters even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials, instructions, methods or ideas contained in the book. Publishing Process Manager Maja Bozicevic Technical Editor Teodora Smiljanic Cover Designer InTech Design Team First published March, 2012 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from [email protected] Advances in Immunoassay Technology, Edited by Norman H. L. Chiu and Theodore K. Christopoulos p. cm. ISBN 978-953-51-0440-7 Contents Preface IX Part 1 New Materials and Assay Interference 1 Chapter 1 Recombinant Antibodies and Non-Antibody Scaffolds for Immunoassays 3 Bhupal Ban and Diane A. Blake Chapter 2 Polyacrylonitrile Fiber as Matrix for Immunodiagnostics 23 Swati Jain, Sruti Chattopadhyay, Richa Jackeray, Zainul Abid and Harpal Singh Chapter 3 Interferences in Immunoassays 45 Johan Schiettecatte, Ellen Anckaert and Johan Smitz Part 2 Label-Free Technologies 63 Chapter 4 Fundamentals and Applications of Immunosensors 65 Carlos Moina and Gabriel Ybarra Chapter 5 Capabilities of Piezoelectric Immunosensors for Detecting Infections and for Early Clinical Diagnostics 81 Tatyana Ermolaeva and Elena Kalmykova Chapter 6 Label-Free Detection of Botulinum Neurotoxins Using a Surface Plasmon Resonance Biosensor 109 Hung Tran and Chun-Qiang Liu Chapter 7 Immunoassays Using Artificial Nanopores 125 Paolo Actis, Boaz Vilozny and Nader Pourmand Part 3 Multiplexing Technologies 141 Chapter 8 Multiplexed Immunoassays 143 Weiming Zheng and Lin He VI Contents Chapter 9 Multiplexed Bead Immunoassays: Advantages and Limitations in Pediatrics 165 Emma Burgos-Ramos, Gabriel Ángel Martos-Moreno, Jesús Argente, Vicente Barrios Preface Over the past decade, the development and applications of immunoassays have continued to grow exponentially. This book focuses on some of the latest advances in immunoassay technology, which include new materials and methods. The book contains nine invited chapters that are divided into three sections. In the first section, the basics for producing recombinant antibodies, the use of polyacrylonitrile fibre as a solid surface, and the nature of interference in immunoassays are summarized. The second section begins with a chapter on the basic concepts of different types of immunosensors, some of which allow label-free detection of specific analytes. This is followed by chapters on piezoelectric immunosensors and surface plasmon resonance biosensors. A chapter on using nanopores as a label-free biosensing platform and its potential for immunosensing is also included in the second section. The third section starts with a chapter that describes different platforms for carrying out multiplexed immunoassays. This is followed by a chapter on the advantages and limitations of multiplexed bead immunoassays. The Editors express their thanks and appreciation to the authors for their contributions to this book project. Moreover, they are thankful to the Editorial Office at InTech for their support. They are also grateful to the love and support from their families, and acknowledge the assistance from their co-workers. Last but not least, they wish to thank all their former teachers and mentors for sharing their knowledge and experience with them. Dr. Norman H. L. Chiu Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, USA Dr. Theodore K. Christopoulos Department of Chemistry, University of Patras, Greece
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