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Chemistry 52 52 Volume Volume Presenting the latest developments in the field for more than four decades, the Advances in Chromatography series is relied on by scientists and researchers for the most up-to-date information on a wide range of chromatographic methods and applications. Volume 52 continues this tradition with contributions by established, well-known chemists, offering cutting-edge reviews of chromatographic methods with applications in the life sciences. Featured topics include • The history, development, and theory behind aerosol-based detectors • Protein and peptide biomarkers that are chromatographic endpoints measured in biological fluids and tissues, such as serum, urine, and tissue biopsies • Multisegment linear gradient optimization strategy in reversed-phase and hydrophilic interaction chromatographic systems • Enantioselective gas chromatography performed using three distinct chiral stationary phases based on hydrogen bonding, metal ion complexation, and inclusion • Analysis of the dynamic phenomena of liquid chromatographic systems with six typical reactions in the mobile phase Providing a clear presentation of topics and vivid illustrations for which this series has become known, the latest volume makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill. 52 EDITORS K22334 Eli Grushka • Nelu Grinberg 52 Volume EDITORS Eli Grushka • Nelu Grinberg Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Group, an informa business CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2014 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Version Date: 20131212 International Standard Book Number-13: 978-1-4822-2352-1 (eBook - PDF) This book contains information obtained from authentic and highly regarded sources. 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Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com Table of Contents Contributors .............................................................................................................vii Chapter 1 Advances in Aerosol-Based Detectors .................................................1 Ryan D. Cohen and Yong Liu Chapter 2 Integration of Analytical Techniques for Protein Biomarker Assays ..................................................................55 Jennifer L. Colangelo Chapter 3 Multilinear Gradient Elution Optimization in Liquid Chromatography .................................................................................79 P. Nikitas, A. Pappa-Louisi, and Ch. Zisi Chapter 4 Analytical Separation of Enantiomers by Gas Chromatography on Chiral Stationary Phases .............................................................117 Volker Schurig and Markus Juza Chapter 5 Analysis of Dynamic Phenomena in Liquid Chromatographic Systems with Reactions in the Mobile Phase ...................................169 Lei Ling and Nien-Hwa Linda Wang v Contributors Ryan D. Cohen A. Pappa-Louisi Analytical Chemistry Department Laboratory of Physical Chemistry Merck Research Laboratories Department of Chemistry Rahway, New Jersey, USA Aristotle University of Thessaloniki Thessaloniki, Greece Jennifer L. Colangelo Drug Safety Volker Schurig Pfizer Worldwide Research Institute of Organic Chemistry and Development University of Tübingen Groton, Connecticut, USA Tübingen, Germany Markus Juza Institute of Organic Chemistry Nien-Hwa Linda Wang University of Tübingen School of Chemical Engineering Tübingen, Germany Purdue University West Lafayette, Indiana, USA Lei Ling School of Chemical Engineering Purdue University Ch. Zisi West Lafayette, Indiana, USA Laboratory of Physical Chemistry Department of Chemistry Yong Liu Aristotle University of Thessaloniki Analytical Chemistry Department Thessaloniki, Greece Merck Research Laboratories Rahway, New Jersey, USA P. Nikitas Laboratory of Physical Chemistry Department of Chemistry Aristotle University of Thessaloniki Thessaloniki, Greece vii