Advanced Techniques in Diagnostic Microbiology PDF
Preview Advanced Techniques in Diagnostic Microbiology
Advanced Techniques in Diagnostic Microbiology Yi-Wei Tang Charles W. Stratton Advanced Techniques in Diagnostic Microbiology Yi-WeiTang CharlesW.Stratton MolecularInfectiousDiseaseLaboratory ClinicalMicrobiologyLaboratory VanderbiltUniversityMedicalCenter VanderbiltUniversityMedicalCenter Nashville,TN37232-5310 Nashville,TN37232-5310 USA USA [email protected] [email protected] LibraryofCongressControlNumber:2005935335 ISBN-10:0-387-29741-3 e-ISBN0-387-32892-0 ISBN-13:978-0387-29741-5 Printedonacid-freepaper. (cid:2)C 2006SpringerScience+BusinessMedia,LLC. Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewritten permissionofthepublisher(SpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork, NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.Use in connection with any form of information storage and retrieval, electronic adaptation, computer software,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden. Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,evenifthey arenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyare subjecttoproprietaryrights. PrintedintheUnitedStatesofAmerica. (TB/EB) 9 8 7 6 5 4 3 2 1 springer.com Contributors JaberAslanzadeh AliDanesh DivisionofClinical DepartmentofExperimental Microbiology Therapeutics DepartmentofPathology UniversityHealthNetwork HartfordHospitalandClinical 200ElizabethStreet LaboratoryPartners Toronto,Ontario,CanadaM5G2C4 85SeymourStreet Hartford,CT06102,USA DianeDare ResearchDevelopmentUnit GeorgeBolton ManchesterMetropolitan BDBiosciences University 10975TorreyanaRoad St.Augustine’sLowerChatham SanDiego,CA92121,USA Street Manchester,UKMI55HA MarkJ.Cameron DepartmentofExperimental PhyllisDella-Latta Therapeutics ClinicalMicrobiologyServices UniversityHealthNetwork DepartmentofPathology 200ElizabethStreet ColumbiaUniversityMedical Toronto,Ontario,CanadaM5G Center 2C4 NewYorkPresbyterianHospital 622West168thStreet SheldonCampbell NewYork,NY10032,USA PathologyandLaboratoryMedicine Service VAConnecticut HelenDeng WestHaven,CTand PublicHealthLaboratory DepartmentofLaboratoryMedicine ArkansasDepartmentofHealth YaleUniversitySchoolofMedicine 4815WestMarkhamStreet NewHaven,CT06520-8035,USA LittleRock,AR72205,USA v vi Contributors WonderDrake UniversityofAlabamainHuntsville DepartmentofMedicine 2707ArtieStreet DivisionofInfectiousDiseases Huntsville,AL35805,USA VanderbiltUniversitySchoolof Medicine XiangY.Han 116121stAve.South DepartmentofLaboratoryMedicine Nashville,TN37232-2605,USA TheUniversityofTexasM.D. AndersonCancerCenter DavidErnst 1515HolcombeBlvd. BDBiosciences Houston,TX77030,USA 10975TorreyanaRoad SanDiego,CA92121,USA RandallT.Hayden DepartmentofPathology TaoFeng St.JudeChildren’sResearchHospital MolecularPathologyLaboratory 332N.LauderdaleStreet DepartmentofPathology Memphis,TN38105-2794,USA MountSinaiSchoolofMedicine, Box1122 MimiHealy OneGustaveLevyPlace BacterialBarcodes,Inc. NewYork,NY10029,USA 8080N.StadiumDrive Houston,TX77054,USA PatriceFrancois DepartmentofInternalMedicine IrvinHirshfield GenevaUniversityHospital DepartmentofBiologicalSciences 21rueMicheli-du-Crest St.John’sUniversity Geneva14,1211,Switzerland 8000UtopiaParkway Jamaica,NY11439,USA StacieR.Frye BacterialBarcodes,Inc. TaoHong 8080N.StadiumDrive DepartmentofPathology Houston,TX77054,USA ClinicalMicrobiologyLaboratory HackensackUniversityMedicalCenter AmitabhGaur 30ProspectAve. BDBiosciences Hackensack,NJ07601,USA 10975TorreyanaRoad SanDiego,CA92121,USA YuanHu U.S.FoodandDrugAdministration TerryJ.Gentry NortheastRegionalLaboratory EnvironmentalSciencesDivision 158-15LibertyAvenue OakRidgeNationalLaboratory Jamaica,NY11433,USA 1BethelValleyRoad OakRidge,TN37831,USA KaiManKam JianHan PublicHealthLaboratoryCentre Genaco 382NamCheongStreet,ShekKipMei DepartmentofPathology Kowloon,HongKong Contributors vii DavidJ.Kelvin ElizabethM.Marlowe DepartmentofExperimental Gen-Probe Therapeutics 10210GeneticCenterDrive UniversityHealthNetwork SanDiego,CA92121-4362, 200ElizabethStreet USA Toronto,Ontario,CanadaM5G 2C4 MichaelL.Pendrak NationalInstitutesofHealth AbdullahKilic Bethesda,MD20892,USA DepartmentofMicrobiology GulhaneMilitaryMedical DesmondPersad Academy DepartmentofExperimental Ankara06018,Turkey Therapeutics UniversityHealthNetwork MarieL.Landry 200ElizabethStreet DepartmentofLaboratoryMedicine Toronto,Ontario,CanadaM5G YaleUniversitySchoolofMedicine 2C4 P.O.Box802035 NewHaven,CT06520-8035,USA RaymondP.Podzorski DepartmentofPathology IvyLee WaukeshaMemorialHospital MolecularPathologyLaboratory 725AmericanAve. DepartmentofPathology Waukesha,WI53188,USA MountSinaiSchoolofMedicine, Box1122 ChaoQi OneGustaveLevyPlace DepartmentofPathology NewYork,NY10029,USA NorthwesternUniversityMemorial Hospital HaijingLi 251EastHuronStreet DivisionofInfectiousDiseases Chicago,IL60611-2908,USA VanderbiltUniversitySchoolof Medicine XuanQin 116121stAve.South DepartmentofLaboratoriesand Nashville,TN37232-2605,USA Pathology AngusC.T.Lo Children’sHospitalandRegional PublicHealthLaboratoryServices MedicalCenter Branch UniversityofWashingtonSchoolof CentreforHealthProtection Medicine DepartmentofHealth 4800SandPointWay,N.E. Kowloon,HongKong Seattle,WA98105,USA MichaelLoeffelholz DietherRecktenwald ViroMedLaboratories/LabCorp BDBiosciences 6101BlueCircleDrive 2350QumeDrive Minnetonka,MN55343,USA SanJose,CA95131,USA viii Contributors JacquesSchrenzel DonnaM.Wolk DepartmentofInternalMedicine DepartmentofClinicalPathologyand ClinicalMicrobiologyLaboratory Medicine GenevaUniversityHospital UniversityofArizona 21rueMicheli-du-Crest DepartmentofPathologyand Geneva14,1211,Switzerland LaboratoryMedicine SouthArizonaVAHealthCareSystem SusanSefers 3601South6thAve. MolecularInfectiousDiseases Tucson,AZ85723,USA Laboratory DepartmentsofPathology FannWu VanderbiltUniversitySchoolof ClinicalMicrobiologyServices Medicine DepartmentofPathology 116121stAvenueSouth ColumbiaUniversityMedicalCenter Nashville,TN37232-5310,USA NewYorkPresbyterianHospital 622West168thStreet CharlesW.Stratton NewYork,NY10032,USA DepartmentsofPathologyand Medicine JosephineWu VanderbiltUniversitySchoolof MolecularPathologyLaboratory Medicine DepartmentofPathology 116121stAvenueSouth MountSinaiSchoolofMedicine,Box Nashville,TN37232-5310,USA 1122 OneGustaveLevyPlace Yi-WeiTang NewYork,NY10029,USA DepartmentsofMedicineand Pathology S.SteveYan VanderbiltUniversitySchoolof FoodandDrugAdministration Medicine Rockville,MD20855,USA 116121stAvenueSouth Nashville,TN37232-5310,USA FeiYe MolecularPathologyLaboratory SiheWang DepartmentofPathology NorthwesternUniversity MountSinaiSchoolofMedicine,Box TheFeinbergSchoolofMedicine 1122 Children’sMemorialHospital OneGustaveLevyPlace 2300Children’sPlaza NewYork,NY10029,USA Chicago,IL60614,USA BingjiaoYin YunF.(Wayne)Wang MolecularPathologyLaboratory DepartmentofPathology DepartmentofPathology EmoryUniversitySchoolofMedicine MountSinaiSchoolofMedicine, GradyMemorialHospital Box1122 80JesseHillDr.,S.E. OneGustaveLevyPlace Atlanta,GA30303,USA NewYork,NY10029,USA Contributors ix DavidZhang Children’sMemorialHospital MolecularPathologyLaboratory 2300Children’sPlaza DepartmentofPathology Chicago,IL60614,USA MountSinaiSchoolofMedicine, Box1122 JizhongZhou OneGustaveLevyPlace EnvironmentalSciences NewYork,NY10029,USA Division OakRidgeNational XiaotianZheng Laboratory NorthwesternUniversity 1BethelValleyRoad TheFeinbergSchoolofMedicine OakRidge,TN37831,USA Preface Clinical microbiologists are engaged in the field of diagnostic microbiology to determinewhetherpathogenicmicroorganismsarepresentinclinicalspecimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. Duringthepasttwodecades,technicaladvancesinthefieldofdiagnosticmicro- biology have made constant and enormous progress in various areas, including bacteriology,mycology,mycobacteriology,parasitology,andvirology.Thediag- nostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecu- lar aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendlysoftwarehavesignificantlybroadenedthediagnosticarsenalforthe clinical microbiologist. The conventional diagnostic model for clinical microbi- ologyhasbeenlabor-intensiveandfrequentlyrequireddaystoweeksbeforetest resultswereavailable.Moreover,duetothecomplexityandlengthofsuchtesting, thisservicewasusuallydirectedatthehospitalizedpatientpopulation.Thephys- icalstructureoflaboratories,staffingpatterns,workflow,andturn-aroundtimeall havebeeninfluencedprofoundlybythesetechnicaladvances.Suchchangeswill undoubtedlycontinueandleadthefieldofdiagnosticmicrobiologyinevitablyto atrulymoderndiscipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diag- nosis of infectious diseases in the routine clinical microbiology laboratory. The bookisdividedintotwoparts.Thefirstpart,“Techniques,”coverstheprinciples andcharacteristicsoftechniquesrangingfromrapidantigentesting,toadvanced antibodydetection,toinvitronucleicacidamplificationtechniques,tonucleicacid microarrayandmassspectrometry.Sufficientspaceisassignedtocoverdifferent nucleicacidamplificationformatsthatarecurrentlybeingusedwidelyinthediag- nosticmicrobiologyfield.Withineachtechnique,examplesaregivenregardingits applicationinthediagnosticfield.Commercialproductinformation,ifavailable, isintroducedwithcommentaryineachchapter.Ifseveraltestformatsareavailable foratechnique,objectivecomparisonsaregiventoillustratethecontrastsoftheir xi
The list of books you might like
