46th Annual Meeting of the American College of Veterinary Ophthalmologists, Coeur d'

18 Pages·2015·0.26 MB·English

VeterinaryOphthalmology(2015)18,6,E17–E34 DOI:10.1111/vop.12319
Abstracts: 46th Annual Meeting of the American College of
Veterinary Ophthalmologists, Coeur d’Alene, ID October 7–10, 2015
POSTER ABSTRACTS
ABSTRACT NO.: 001 increasededemaandprogressivevascularizationwereseenonpost-operativeday16.However,
60%ofthexenograftareahadimprovedtransparency3monthsaftersurgery.UsingOCT,
Initial clinical experiences with bilateral partofthexenograftwasthickenedandirregularcomparedtotheperipheralcornea.Using
IVCM,thecentralcorneadisplayedlossoflamellarorganizationwithreplacementbyaran-
phacoemulsification in captive Macaroni (Eudyptes dom,haphazardarrangement.Onhistopathologicalexamination,thexenograftwasinfiltrated
Chrysolophus) and Southern Rockhopper (Eudyptes withinflammatorycells.ThirtypercentofthecorneadisplayedstromaldegenerationandDes-
cemet’s membrane rupture.Conclusions: Clinical and histologic rejection of pig-to-dog
Chrysocome) Penguins penetratingcornealxenograftoccurred,however,itachievedtectonicstabilityand60%of
GHSila,*SMAquino,*GFuller,‡SAllard‡andSJWoodhouse† xenografthadimprovedtransparencyby3monthsaftersurgery.None.
*DepartmentofOphthalmology,BluepearlVeterinaryPartners– ABSTRACT NO.: 004
†
Michigan; DepartmentofAnimalHealthandNutrition,Detroit Intraocular mast cell tumors in dogs
‡
ZoologicalSociety; CenterforZooAnimalWelfare,DetroitZoological BBeckwith-Cohen*andRRDubielzig†
Society *VCAWestLosAngelesAnimalHospital,LosAngeles,CA,USA;†The
Purpose: Todescribesurgicaltechniqueandoutcomesin2southernrockhopperand3 ComparativeOcularPathologyLaboratoryofWisconsin,Schoolof
macaroni penguins undergoing bilateral phacoemulsification.Methods: Avisual penguins
withhypermaturecataractswereidentifiedfromacaptivepopulation.Combinedrod-cone VeterinaryMedicine,UniversityofWisconsin,Madison,WI,USA
standardflashelectroretinographyandocularultrasoundwereperformed.Phacoemulsifica- Purpose: To describe the morphology of intraocular mast cell tumors in dogs.Meth-
tionwasperformedviaa3.2mmincisionusingaone-ortwo-handedtechnique.Incisions ods: TheCOPLOWdatabasewassearchedforintraocularmastcelltumorsindogsand
wereclosedwith9-0vicrylmonofilamentsutureincontinuousorinterruptedpattern.Topi- cats.Histologicalslideswerestainedwithhematoxylin-eosinandgiemsa.Immunohistochem-
claamlapndbisoymstiecmroisccoanptyi-iannfldamrembaotuonrdiestoannodmanettribyiowtiecrsewpeerrefoardmmeidnisatter1ed–2pwosete-kopienrtaetrivveallys.uSnlitti-l iisdternytiwfiaesdpienrfdoormgse,danfodrnCoDin-1tr1a7o.cuRleasrumltass:tcFeolulrtucmasoerssowfeinretraidoecnutliafiremdainstccaetlsl.tOumfothrsewfoeurer
inflammationresolvedandmedicationswerediscontinued.Behavioraldatawascollectedfor tumorsonewasinapatientwithametastaticgrade3mastcelltumorandwaspresumedto
4individualsfor2weekspreandpostsurgery.Results: Aspirationthroughthephaconee- beametastaticlesionandhadamitoticindexof5perhighpowerfield.Threecaseswere
dlewassufficienttoremovethemajorityofthelensmaterialinalleyes.In9outof10eyes presumed to be primary intraocular lesions. The presumed metastatic lesion extended
thelenscapsulewascompletelyluxatedorseverelysubluxatedandremovedviathecorneal throughouttheanteriorandposterioruvea,exfoliatedintotheanteriorandposteriorcham-
incision.Postoperativeuveitiswasminimalinalleyes.Inflammationalongthedorsolateral bersandinvadedthesclera.Thepresumedprimarymasslesionsaffectedpredominantlythe
edgeofthemembrananictitans(MN)developedinalleyes.Defectsofthedorsaledgeof anterioruvea,twoofwhichinfiltratedthechoroid;onewasasolitarylesionintheciliary
theMNdevelopedin6of10eyesduringthefirstpost-operativeweek.OneMNalsodevel- body.Giemsahighlightedmetachromaticgranulesinneoplasticcellsof3/4cases,including
opedafull-thickness5mmcentralhole.MNhealingcorrelatedwithsuturedissolutionand themetastaticlesion,andCD-117labeledpositiveinallcases.Lymphoplasmacyticuveitis
wusaesocfomthpelehteabwitiatthisnpa8cew(epek<s0in.05a)llaenydesf.eeBdeihnagviboerhalavdioarta(psh<ow0e.0d5)apsoigsntifisucragnetryin.cCreoansceluin- worascplirneisceanlteivnidaellncceaseosfagnldauecoosminao.pChiolsnwcleurseiosneesn:inCa3n/4inceasienst.raAolclufloaurrmcaassetschealdlhtuismtoolrosgiacrael
sions: Phacoemulsificationcarriesagoodprognosisinthesespeciesandimprovesactivity rarelesionsindogsandtherearenoexamplesofthesetumorsincats.None.
level.MNdefectsmayhavebeenrelatedtosutureirritationandsmallersuturegaugeor
alternativecornealclosuremethodsshouldbeconsideredforthesespecies.None.
ABSTRACT NO.: 005
ABSTRACT NO.: 002 Normal bacterial conjuctival flora in the American
Use of N-butyl cyanoacrylate adhesive for the treatment Bison (BIson bison)
of erosive lipid keratopathy in two Panamanian Golden GBen-Shlomo,*RJMadison,†DEFligg,‡JMKinyon,‡TFrana‡
†
Frogs (Atelopus zeteki) andSOlsen
SMAquino,*WCShellabarger†andMVasallo‡ *DepartmentofVeterinaryClinicalSciences;†CollegeofVeterinary
*DepartmentofOphthalmology,BluePearlVeterinaryPartners,MI; Medicine,IowaStateUniversity,Ames,IA.UnitedStatesDepartmentof
† ‡ Agriculture,AgriculturalResearchService,NationalAnimalDisease
VeterinaryMedicalCenter,DetroitZoologicSociety; Amphibian ‡
Center,Ames,IA,USA; VeterinaryDiagnosticLaboratory
DepartmentKeeper,DetroitZoologicSociety
Purpose: TodescribethebacterialfloraofthenormalconjunctivaoftheAmericanBison
Purpose: Describetheuseoftissueadhesivefortreatmentoferosivelipidkeratopathyin (Bisonbison).Methods: Conjunctivalswabswerecollectedfrombotheyesof15American
twoPanamianGoldenFrogs(PGF)(Atelopuszeteki).Methods: TwoPGFswerepresented bisons,followingacompleteophthalmicexamination.Theswabswereculturedforaerobic
with bilateral corneal disease. Both had unilateral corneal rupture and contralateral deep andanaerobicbacteria,andfungi.Identificationwasperformedprimarilyusingmatrix-as-
axialstromal loss.Frogswere anesthestizedfor unilateralenucleationof rupturedglobes. sistedlaserdesorptionionizationtime-of-flight(MALDITOF)massspectrometryandsup-
TreatmentwithN-butylcyanoacrylatewaselectedforthecontralateraleyeswhichwerecon- plementedwithconventionalmethods.Results: All15animalswerefoundtobefreeof
sideredatriskforrupture.Asingledropofproparacainewasappliedtopicallytoeyeswith oculardiseasethatmayaffecttheresultsoftheculture.Atotalof11bacterialgenera,and
stromalloss.Weck-Celspongeswereusedtolightlydebrideandthoroughlydrycornealsur- nofungalisolateswerecultured.ThemostfrequentbacteriaisolateswereArthrobacterspp.
fsawcaebs.aAndsinapgplelidedrowpiothfNa-dbaubtbyilncgyamnootaicornylcaotevewriansgptlahceedareoanothfesttriopmoaflalocslseaann,ddreyxtWenedcinkg-C3e–l EanndterAcocicncuetsobspapct.e,rEsspcph.erOicthhiaercbolai,ctSearicachianrcoltuhdriexdsPyrseinugdaome,oJnoansesspiap.,dePnaintrtiofieacasnpsp,.,BSatcailpluhsylocecorcecuuss,sapnpd.,
4mm beyond the affected area.Results: Enucleation sites healed without complication. Mirobacterium keratanolyticum.Conclusions: To the authors’ knowledge, this is the first
Histopathologyofenucleatedeyesconfirmedthediagnosisoflipidkeratopathy.Tissueglue reportofthenormalbacterialconjunctivalfloraintheAmericanBison(Bisonbison).None.
re-applicationwasrequiredat1week.Subsequentretentionwasgoodwithtissuegluestill
adherentat2and4weekspostprocedure.Theaxialcorneasofbothfrogswerethickened
postadhesiveextrusionasassessedbyslitlampbiomicroscopyindicatingimprovedintegrity. ABSTRACT NO.: 006
Nocornealvesselswerenotedbyslitlampbiomicroscopyatanytimeperiodpreorpost Complex maxillary malformation with orbital zygomatic
operatively.Norecurrenceofcorneallesionswasnoted.Serialbodyweightmeasurements
were stable to increased over the 1year follow up period.Conclusions: N-butyl mucocele in a puppy: a case report
ccyoarnnoeaalcrinyltaetgeriitsy.anPGefFfescutirvveivtarleiastmnoenttafffoerctperdobgyreussnivileatleirpaildekneurcalteoaptiaothny.Nwoitnhec.ompromiseof ACirla,*MRondena,†CStefanello,*LAngeloni,*RRomanelli,*
GBarsotti‡andGBertolini*
ABSTRACT NO.: 003 *ClinicaVeterinariaPrivataSanMarco,Padova,Italy;†Laboratorio
‡
Penetrating corneal xenotransplantation of SPF pig d’AnalisiPrivatoSanMarco,Padova,Italy; DipartimentodiScienze
corneas to a dog: a preliminary study Veterinarie,Universit(cid:1)adiPisa,SanPieroaGrado,Italy
JHBae,*JKim,*,†DBJ???,†NSKim*andMSKim* Purpose: Todescribeazygomaticglandmucoceleandconcomitantmaxillarymalforma-
*CollegeofVeterinaryMedicine,ChonbukNationalUniversity;†JiDong ttihoanlminosawdiothg.tAhir7d-meyoenltihd-porldo,trmusailoen,manixdedabgrreaedduadlolygehnaldargaerdecsewnetl-loinngseitnotfhuenriliagthetratlemexpooprha-l
BeomAnimalHospital region.Methods: Physicalandophthalmicexaminations,imaging,surgeryandhistologyof
the lesion were performed.Results: A compressing, painful right retrobulbar mass was
Purpose: ToexaminetheeffectivenessofYorkshirepig(Large-WhiteXLandrace)cornea clinicallysuspectedandconfirmedbyultrasound.Computedtomographyrevealedalarge
forfullthicknesstransplantationtoadog.Methods: Penetratingcornealxenotransplantation multiloculated cystic-like lesion of the right zygomatic gland projecting into the orbital
wasperformedusingSPFYorkshirepigcorneatoadog.Topical2%cyclosporinewasapplied space,displacingtheeyeball.Theipsilateralmolarpartofthemaxillarybonewereunderde-
threetimesdailyfor4weekspostoperatively.Xenograftwasevaluatedbyslitlampmicroscopy, velopedwithmultipleabnormaluneruptedmandibularcrowdedmolarteethinthepremolar
ocular coherence tomography (OCT) and in vivo confocal corneal microscope (IVCM) region. Modified lateral orbitotomy and selective caudal maxillary bone access were per-
3monthsaftersurgery.Therecipienteyewasenucleatedforcornealhistopathologicalstaining formed to provide ventral exposure of the orbit and dental extraction, while minimizing
91daysaftersurgery.Results: Characteristicsofimmunerejectionofthexenograftwith traumatotheglobeandsupportingstructures.Thepseudocystwasremovedandthewall
©2015AmericanCollegeofVeterinaryOphthalmologists
E18 abstracts
sampledforhistology.Sectionswerecharacterizedbyaciniofzygomaticglandembeddedin todetectandcharacterizesuperficial,deep,ulcerative,ornon-ulcerativecorneallesionssec-
athickgranulationtissuedelineatedinaborderbyabundantfibrinandamorphoushyaline ondarytobothorganicandnon-organiccornealforeignbodiesindogs.None.
homogeneous material (salivary secretion), partially infiltrated by macrophages consistent
withsialocele.Clinicalandimagingexaminationssixmonthsaftersurgeryshowednorecur- ABSTRACT NO.: 010
renceofthemucoceleorocularimplications.Conclusions: Wedescribedacomplexcase
ofexophthalmosduetozygomaticmucoceleinapuppywithipsilateralmaxillaryhypoplasia. Corneal-protective effects of tear substitutes containing
Apossiblerelationshipbetweenthesetwoconditionscouldbehypothesizedonthebasisof
advancedimagingfeatures.Earlydiagnosisandmanagementpreventedlaterocularcompli- sodium hyaluronate (SH)/dodecahydrosqualene (DHS)
cations.None. with poly-vinyl alcohol (PVA) on porcine dry eye model
ABSTRACT NO.: 007 and assessment of adverse effects of the substitute in
Establishing normals for and validating the Pentacam- live rabbit eyes
HR(cid:1) for anterior segment tomography in healthy feline THasegawa,*HAmko,*YSakamoto†andSSawa†
eyes *OsakaPrefectureUniversity;†Senju,PharmaceuticalCo.Ltd.
AMCleymaet,SRaoandKSFreeman Purpose: Topreparelong-actingtearsubstitutescontainingsodiumhyaluronate(SH)/do-
decahydrosqualene(DHS),thecorneal-protectiveeffectsofthesubstituteswithorwithout
DepartmentofClinicalSciences,CollegeofVeterinaryMedicine,Colorado polyvinyl alcohol (PVA) were assessed on an in vitro enucleated porcine dry eye model
StateUniversity (ivEPDRM)andtheadverseeffectsofthesubstituteofSH/DHSwithPVAwerealsoevalu-
atedonliverabbiteyes.Methods: Thecorneal-protectiveeffectsof0.1%or0.3%SH/
Purpose: Tomeasurecentralcornealthickness(CCT)andanteriorsegmentparametersin 5%DHSwithorwithoutPVAwereevaluatedusingivEPDRMwithmethyleneblue(MB)
healthyfelineeyeswiththePentacam-HR(cid:1),arotatingScheimpflugcamera,andtocompare or lissamine green (LG). Adverse effects of 0.3% SH/5% DHA with 0.1% PVA were
theseCCTvalueswiththoseobtainedwithaspectral-domainopticalcoherencetomography assessedbyophthalmicexaminationsofliverabbiteyesadministeredthedrug.Results: 0.1
(SD-OCT) device, the Optovue(cid:1) iVue.Methods: Repeated CCT measurements were %PVAsupplementationresultedintheincreasedcorneal-protectiveeffectsinthetearsub-
obtainedfrombotheyesof35healthydomesticshorthairedcatsusingSD-OCTandPenta- stitutesofboth0.1%SH/5%DHSand0.3%SH/5%DHS.Therewerenosignificantdif-
cam-HR(cid:1).AdditionalanteriorsegmentparameterswereevaluatedwithPentacam-HR(cid:1).The ferencesinMBandLGstainingbetweencontroleyestreatedcontinuouslywithsalineand
continuousdataonCCTandagewerecomparedbetweenthemodalitiesandbetweeneyes 0.1%SH/5%DHSwithPVAat90minutesofdesiccationonivEPDRM,whereasnosignif-
usinglinearregressionanalysisforassociation.Bland-Altmananalysiswasusedtoevaluate icantdifferenceswerefoundinMBandLGstainingbetweencontroleyesandonestreated
agreement between the imaging modalities for CCT.Results: Mean(cid:1)SD CCT was with0.3%SH/5%DHSwith0.1%PVAat240minutesofdesiccationinthemodel.Mild
589.1(cid:1)42.9lmforODand586.9(cid:1)40.4lmOSusingSD-OCT,withnosignificantdif- conjunctival hyperemia were temporally observed in rabbit eyes administered the 0.1%
ferencebetweeneyes.Mean(cid:1)sdCCTwas604.1(cid:1)61.6lmforODand603.7(cid:1)77.1lm PVA/0.3%SH/5%DHSsolution4timesperdaywith2–3hintervalsfor5days.Conclu-
OSusingPentacam-HR(cid:1),withnosignificantdifferencebetweeneyes.Flattestkeratometric sions: Thesefindingsindicatethatthetearsubstituteof0.3%SH/5%DHSwith0.1%
valuewas43.1(cid:1)71.2diopters(D).Steepestkeratometricvaluewas66.7(cid:1)317.1D.Ante- PVAwouldbeusefulasalong-actingartificialtearfortreatinganimalswithtearfilmabnor-
rior chamber depth was 4.4(cid:1)1.0mm and angle was 36.1(cid:1)19.6(cid:3). Corneal volume was malitiessuchaskeratoconjunctivitissicca.None.
59.5(cid:1)57.4mm3. Astigmatism was 45.3(cid:1)335.8D. There was a significant difference
betweenPentacamandOCTvaluesforCCTwithOCTvaluesonaverage15.9lmlower ABSTRACT NO.: 011
thanPentacamvalues(P=0.02).Therewasmoderateagreementbetweenmodalitiesbased
onBland-Altman.Conclusions: ThePentacamprovidesvaluableanteriorsegmentparame- Canine T4R RHO model lacking 11 cis-retinal is not
tersinfelineeyes.Thereismoderateagreementbetweenmodalities,withCCTSD-OCT
readinganaverageof15.9lmlowerthanPentacam-HR(cid:1).SupportedinpartbytheCenter sensitive to light damage but undergoes earlier and
forCompanionAnimalStudiesatColoradoStateUniversity.None. faster retinal degeneration
ABSTRACT NO.: 008 SIwabe,RSudharsan,GDAguirreandWABeltran
Single dose effect of topical ophthalmic dorzolamide SectionofOphthalmology,DepartmentofClinicalStudies,Schoolof
VeterinaryMedicine,UniversityofPennsylvania,Philadelphia,PA,USA
hydrochloride 2%/timolol maleate 0.5% on intraocular
Purpose: Toexaminedwhethergeneticablationof11-cis-retinalthroughtheRPE65null
pressure in normal dogs genotypepreventsacutelight-inducedphotoreceptorlossinthismodelofclassB1RHO-
EDDolan,IPHerringandYVasilyeva adRP.Methods: Littermatedogswithdistinctgenotypeswereusedinthisstudy,includ-
ing:T4RRHOaffected,double-affectedandnon-affecteddogs.At7wkofage,underanes-
Virginia-MarylandCollegeofVeterinaryMedicine thesiathedogshadtheirlefteyeexposedtoasingledoseofwhitelight(cornealirradiance
of1mW/cm2for1min)withamonocularGanzfeld.Therighteyewasshieldedandused
Purpose: To determine the effect of single dose topical ophthalmic dorzolamide
as control. ONL thickness was measured by non-invasive cSLO/sdOCT imaging before
hydrochloride 2%/timolol maleate 0.5% (DHTM) (Hi-Tech Pharmacal Co., Inc., Ami- (6wkofage)and2wkafterlightexposure(LE)(9wkofage).Results: PriortoLE(6wk
tyville,NY11701)onintraocularpressure(IOP)innormaldogs.Methods: Arandomized, ofage)theONLinbotheyesofdouble-affecteddogwassignificantly(~50%)thinnerthan
masked,placebo-controlledcrossoverstudywasperformedusing12clinicallynormalbeagles
dividedintotwotreatmentgroups(n=6pergroup).12-hbaselineIOPswereestablished tilhaartdoefctrheeasTe4(R~2R0%HO)ianffeOctNedLatnhdicnkonremssalindobgost.hBethtweeeexnp6osaendda9ndwkthoefsahgieeltdheedreewyeassoafsitmhe-
pDrHioTrMtootrreaatmceonntt.roDlosgosluitnioneac(BhlignrkouCponretaccetisv(cid:1)ed, AobnbeotttreaLtambeonrattoinriebs,otAhbebyoetst oPfareki,thIeLr dthoeubelxep-aofsfeedcteedyedoogf.tAheseTve4rReRthHiOnnianfgfecotfedthedoOgN. TLh(e~8O5N%L)wthasickseneenssdufrroinmgtthheatTpe4rRioRdHiOn
60064).IOPsweremeasuredat0,2,4,6,8,10,and12hpost-treatment.Aftera72-hwash- (shielded) and the non-affected (shielded/exposed) retinas decreased by only ~ 7%, and
outperiod,treatmentgroupswerereversed.Results: TreatmentwithDHTMresultedina
waslikely dueto age-related thinning of the ONLassociated witheye growth.Conclu-
significantIOPdecreasecomparedtothecontrolsolutionandbaselinevaluesatalltime
sion: Ourresultsshowthatsignificantlossofphotoreceptorsisalreadypresentasearlyas
pointsbeginning2hafteradministration.Peakeffectwasobserved6haftertreatmentand at6week-olddouble-mutantRHOT4R/+/RPE65-/-,butthatitisnot-sensitivetolight-dam-
continuedthroughoutthestudyperiod.Time0IOPforthecontrolgroupintreatmentper-
age. These findings further support the claim that 11-cis retinal is critical in stabilizing
iod2wasalsosignificantlydecreasedcomparedtobaselineIOP,time0IOPinbothDHTM
mutantT4Rrodopsin,andthatreleaseofthechromophoreuponphoto-activationmaylead
treatmentgroupsandtime0IOPforthecontrolgroupinperiod1.Conclusions: Innor-
toachangeinconformationoftheapoproteinthatdisruptsthestructuralintegrityofdiscal
motensivecanineeyes,topicaladministrationofDHTMeffectivelylowersIOPwithin2h membranes.SupportedbyNIH/NEIEY06855,EY17549,PN2EY018241, R24EY022012,
ofadministration,reachingpeakeffectby6h,withadetectableIOP-loweringeffectlasting
>72h.FurtherstudyiswarrantedtodeterminetotaldurationofactionofDHTMindogs. R24EY023937,P30EY001583,FFB.CR:None.
SupportedbyVirginia-MarylandCollegeofVeterinaryMedicine,DepartmentofSmallAni-
malClinicalSciences.None. ABSTRACT NO.: 012
Morphological characterization of cells attached to
ABSTRACT NO.: 009
anterior capsules obtained using phacoemulsification
In vivo confocal microscopy of corneal foreign bodies
in dogs with mature cataract
in dogs: a case series NKanemaki,*,†MInaniwa,*YIchikawa,†KTerakado*and
AMEndersandECLedbetter ‡
HOchiai
DepartmentofClinicalSciences,CollegeofVeterinaryMedicine,Cornell *VeterinaryTeachingHospital;†DepartmentofVeterinaryMedicine;
University,Ithaca,NY,USA ‡
ResearchInstituteofBiosiences,AzabuUniversity,Japan
Purpose: Tocharacterizetheinvivoconfocalmicroscopicfeaturesofdogswithmacro-
scopicandmicroscopiccornealforeignbodies.Methods: Sevenclinicalcasespresentedto Purpose: Weinvestigatedthemorphologicalstructuresandmineralelementalcompositions
the Cornell University Hospital for Animals (Ithaca, NY) with corneal foreign bodies ofthecellsattachedtoanteriorcapsulesindogswithmaturecataracts.Methods: Forty-two
detected on clinical examination or clinical findings consistent with foreign bodies were anteriorcapsulesfrom42dogswithprimarymaturecataractwereobtainedbyphacoemulsi-
examinedbycornealinvivoconfocalmicroscopy.Thediagnosisandtypeofcornealforeign fication.Withtheapprovalofouruniversity’sanimalresearchcommittee,theanteriorcap-
bodywereconfirmedbyclinicalexaminationorhistopathology.Alldogsreceivedacomplete sules of 5 clinically healthy beagles were removed immediately after the dogs were
ophthalmicexaminationpriortoinvivoconfocalmicroscopythatincludedslit-lampbiomi- euthanizedotherthanforthestudypurposesandusedasnormal,controlcapsules.Anterior
croscopy,indirectophthalmoscopy,SchirmerIteartesting,applanationtonometry,andfluo- capsuleswerestainedwithtoluidineblueandobservedbyphase-contrastmicroscopy.Cells
resceinstain.InvivoconfocalmicroscopywasperformedwithamodifiedHeidelbergRetina attachedtotheanteriorcapsuleswereobservedbyscanningelectronmicroscopy(SEM)with
TomographIIandRostockCorneaModuleusinga639objectiveand400lmfieldlens. Hitachiionicliquidforpreventingcharge-up,andanalyzedusingenergy-dispersiveX-ray
Examinationswereperformedusingonlytopicalanesthesiaandmanualrestraint.Following spectroscopy(EDS).Results: Thecelltypesobservedintheanteriorcapsulesofdogswith
examination,retaineddigitalimageswereanalyzedforpathology.Results: Invivoconfocal maturecataractwereclassifiedascuboidal,spindle,fiber-like,pyknotic,andnecrotic,while
microscopywashelpfulinallcasestoestablishadiagnosisandcharacterizecornealpathol- thoseseeninthecontrolcapsuleswerecuboidalandpyknotic.SEMrevealedthattheinter-
ogy. Analyzed images showed evidence of stromal leukocyte infiltration, anterior stromal cellularspacesofepithelialcellsinmaturecataractswereloosenedmorethanthoseseenin
dendritic cell proliferation, and corneal vascularization. Identified corneal foreign bodies thecontrolcapsules.EDSshowedthepresenceofcalcium-richparticlesattachedtocellson
includedbonefragments,unidentifiedplantmaterials,aseedhull,metallicbulletfragments, the anterior capsule in mature cataracts.Conclusion: Morphological changes of lens
andawoodenthorn.Alldogsmaderecoveredfollowingsurgicaland/ormedicalinterven- epithelialcellsinmaturecataractsmayperhapsbethecauseofthepresenceofcalcium-rich
tion.Conclusions: Invivoconfocalmicroscopyprovidesanexcellentdiagnostictechnique particles,ascalciumisknowntobeelevatedinmostlenseswithcataracts.None
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E34
abstracts E19
ABSTRACT NO.: 013 withvisionlossduetobilateralmaturecorticalcataracts.Theotherthreewere3year-old
Alouattacaraya,orphanstotallyblindduetoanutritionalmaturecataract.Acompleteoph-
Bilateral phacoemulsification followed by an acrylic thalmicexam,electroretinogramandaB-modescanocularultrasonographywasconducted
foldable intraocular lens implantation in a 2.5 year-old onallanimalsandrevealednoevidenceofretinaldiseases.Nolensinstabilityorclinically
importantlens-induceduveitiswasnoted.Cataractsurgerywasplannedandperformedat
Bengal Tigress (Panthera tigris tigris): case report twodifferentpractices.TheAlouattafuscawasperformedbyonesurgeon,oneeyeatatime
JAKleiner,*EWatanabe†andCBettini† adnayd.tIhnetrtahorceuelaArloleunatsteasc(aIrOayLass)bwyearenoitmheprlasnutergdeoanndbontohceoymesplaictatthioenssamdueritnimgethoenptrhoecesdaumree
*VetwebOftalmologiaVeterin(cid:3)aria,Curitiba,Brazil;†ProntodogSmall werenoticed.Theintraocularlenseswerecustommadeandtheirspecificationscalculatedby
AnimalHospital,Maringa–Pr voicsuiolanrounltrtahseonnoexgtradpahyyaaftnedrtchoernperaolcceudruvraetsuarendmneoasmuraejmorencotsm.pRleicsautlitosn:soAbllsearnviemdailnsorengeaiyneeadr
Purpose: This case report intends to describe the preparation, technique and results followedupperiod.Conclusions: Totheauthorsknowledgethisisthefirstreportonpha-
obtained in a bilateral cataract surgery utilizing phacoemulsification and foldable acrylic coemulsificationandIOLsimplantationinhowlermonkeys.Thesespeciesareamongendan-
intraocular lens in Bengal Tigress.Case report: A 2.5year-old intact female Bengal gered ones and considering the successful procedures and visual rehabilitation they were
Tigresswasexaminedduetoseverevisualdeficitsandaftersedationacompleteeyeexam releasedintothewild.Commercialinterest: None.
revealedabilateralmaturecataract.Ultrasound,electroretinography,tonometryandcom-
plete blood work were performed before the surgeryand were withinthe normallimits. ABSTRACT NO.: 017
Bimanualphacoemulsificationtechniquewasutilizedandbotheyesoperatedatsameproce-
dure.Intraocularcustommadelensesspecificationswere:powerof30D,overalllengthof Noncontact specular microscopy following the use of
21mm,12mmbiconvexopticalzoneandhapticangulationof00.Comments: Anterior intracameral ascorbic acid during phacoemulsification
capsulewasveryelasticandthinmakingthecapsulorhexiseasier.Centralpartofthelens
wasveryhardandtookalongtimeforfragmentationandaspiration.Intraocularlenswere in dogs with senile cataract
iannjteicbtieodticisntaondthaentcia-ipnsflualamrmbaatgoruiessi.ngNoa Efollidzainbgethfoarncecposl.laProwsta-sopuetirlaiztievdebdercuagussecothnesisatnedimoafl IRMPadua,*TBLima,*GPValdetaro,*RMCrivelaro,*
wastrainingbeforetheproceduretogetusedtoeyedrops.Noimportantinflammationwas KKKobashigawa,*CPBalthazar,*PESSilva,*MAldrovani*and
observed after the procedure and the patient was totally visual 3days after the proce- JLLaus*
dure.Commercialinterest: None.
OphthalmologyUnit,DepartmentofMedicineandSurgery,Facultyof
ABSTRACT NO.: 014 AgrarianandVeterinarySciences,UNESPJaboticabal,SP,Brazil
Hurricane keratopathy in an 11 year-old Shih-Tzu: case Purpose: Evaluate the antioxidant role of intracameral ascorbic acid on the corneal
endotheliumofdogssubmittedtophacoemulsification.Methods: Theeyesof10dogswith
report bilateralsenilecataractswereused.Sterileascorbicacidwasaddedtotheirrigationsolution
JAKleiner used during phacoemulsification to make a final ascorbic acid concentration of 0.001M.
VetwebOftalmologiaVeterin(cid:3)aria.Curitiba–Brazil. (E%nd)oatnhdelitahlecceollefdfiecniesnittyo(fcevlalsr/imatmio2n)(a%nd)ianrecael(lmsimze2),wceorerneevaalluthatieckdnbesysn(omnmco),nthaecxtasgpoencaulliatyr
Purpose: Thiscasereportintendstodescribeararecaseofhurricanekeratopathyinan microscopy(TopconSP3000P).Evaluationswereconductedpriortothesurgicalprocedure
1ecatthoirx1atpeailmyific1tecair2ainalarteuh-lsdomtteldeddaraaoturyiisecSd.thstuhRoilehpecs-lceceuThrovsezertcnuahrker.eatatwiCofitcawacrusesialaeadsaltromttrnopeeeetaanapri6lnstolyrpdpitnrarh:eeyetssshaccAlelrrenaiidbbtreieegeraddhlneatdwfnvoeedaranyseh3c.teyloaoAwepluaieincrdceo-gaikonmlslpedapsrwn,soleteciotsrebepefsiaosetpythywerieecedasses,ccxofrcaaeiroubmmnnteeoasdarill.edoevwegAeroaSfaeutshldeesirpdhnsr-aoeeaTrsr4uemszunmuwaptlee.aawreBnnfikaddy-ss d1(6winM80oict4t0rh(h3eM)e,al(cid:1)ns5iieaom)dl2amc5snsei1eigdlg.dln1ni9diaifi0fiaetcetnc(alasMyMninttati63elf)y,dts.ei1waDrff8tee(i6fMrrMf1eeen15r(cid:1)2ce)1e(na50s2cn812ed1b9s(cid:1).e.0wo9twn2e(cid:1)ar2etpee28onM.c92sot.44oPan,2tpsOi)e1MdDr8cea20orst9e,imvd2ce(cid:1)po2samd9ir1g4aep5nyda(cid:1)0isfir.we6(cP2diat7aOnh0twtD.MM0witshh)a05te7(nMaM4n(7pM0d13,<.215(1)p80,9(cid:1)5.8>10074560(cid:1).5(cid:1).(0M.R85224e)373.;s)93u,p.C.1l22te<8sala:ltt0(M.MMaE0r45ne26))a-,.,,
oofftthheecinoirtniaelaparnedseantsalitgiohntflaucolroecskcweiinseuvpotartkeexi(dwenhtoirfilepdaattnedrna)dwiaagsnnoostiiscoedfhounrrtihceanceenkterraaltoppaar-t Rfeeregnarcdeinwgascovrenreifialedthbicektnweesesn,hPeOxaDgosn(aplit>y0a.n0d5)t.hCeoconecflfiucsiieonntso:fNvaoriastiigonnifiincacnetlllossizsee,sninodceifl-l
thy made.Comments: Hurricane keratopathy is the name given to the whorl pattern, density,cornealthickness,hexagonalityandcoefficientofvariationwereobserved,withthe
highlightedwithfluorescein,seeninsituationswherecornealepithelialcellturnoverisexag- additionofintracameral0.001Mascorbicacidintheirrigationsolutionusedduringpha-
gerated.Inhumansitdiffersfromcorneaverticillatainthatthepatternishighlightedbyflu- coemulsification.AnincreaseincellareawasonlyverifiedforM5.SupportedbytheCNPq
oresceinuptakeanddoesnotrepresentthedepositionofanysubstance,andthecausesare (Proc.300833/2010-5)andtheFAPESP(Proc.2011/20041-8).None.
cornealgraftsandchronicepithelialbreakdownandhealing.Thehypothesisforthepat-
ternedobservedisprobablytheresponseofcornealepithelialcellstoocularelectromagnetic ABSTRACT NO.: 018
field.Commercialinterest: None. Immunophenotypic patterns, chromatin functional
ABSTRACT NO.: 015 states and amounts of DNA from limbal epithelial cells
Trans-scleral diffusion and comparative ex-vivo grown on synthetic versus human amniotic membrane
permeability of dexamethasone in equine, porcine MAldrovani,*KKKobashigawa,*GPValdetaro,*IRMPadua,*
and rabbit sclerae PCCristovam,†JAPGomes†andJLLaus*
PPKo,*JMoreno,†RBrito,†MVargas-Doughery,†RMeredith* *OphthalmologyUnit,DepartmentofMedicineandSurgery,Facultyof
† AgrarianandVeterinarySciences,UNESPJaboticabal,SP,Brazil;
a*nEdyeRCAarCeaFrovralAhnoimals;†3TOphthalmics P†Oauculola,rUSNuIrFfaEcSePAdSv~aaonPceadulCo,enStPer,B(CraAzSilO),FederalUniversityofS~ao
Purpose: Tocharacterizeexvivotransscleraldiffusionofdexamethasonesodiumphosphate
(DEX,Upjohn,Kalamazoo,USA)andcompareitsdiffusionandpermeabilityprofileacross Purpose: Amniotic membrane (AM) has been used for expanding limbal epithelial cells
equine,porcineandrabbitsclerae.Methods: Thefreshscleraewereharvestedandsam- (LECs).Thereare,however,certainconcernswithAM,includingitsstructuralheterogene-
plesweremountedbetweenthedonorandreceptorcompartmentsontothecurvedsurface ityandmethodofmanipulation.Tocircumventtheseconcerns,syntheticmembraneshave
ofFranzcellsandmaintainedat37°C.Solutionscontaining10and100mg/mLofDEX beenproposedasreplacementsforAM.Evidencesuggeststhatmembranesfromchitosan
weredispensedintothedonorcompartments.Sampleswerecollectedfromthereceptorsites (Chi),alginate(Alg)andfucoidan(Fuc)arecandidatesforlimbaltherapy.Thisstudyevalu-
attimeintervals.Thescleraeandresidualsolutionsinthedonorchamberswerecollectedat atedimmunophenotypicpatterns,chromatinfunctional statesandamountsofDNAfrom
tchheroemnadtoogfraepahcyh.rTuhne.flTuhxe,pceorlmlecetaebdilistaymcpoleefsficwieenret(aPnaaplpyz)eadndbyefafechtiigvehdpieffrufosiromnancoceeffilicqiuenidt LbrEanCessgwroerwenfaobnricmateemdbbrayneionfriocmanAdlgt-hCerhmi-aFlucretaincudlaAtiMon..MCerythooprdess:ervCehdi-(A(cid:3)lg8-0F°uCc)minetamc-t
were calculated. Permeability parameters were compared with t-test statistical analysis human AM was obtained from the tissue bank of the CASO-UNIFESP. LECs were
(p<0.05).Results: DEXhaddiffusedthroughallspeciessclerae.Rabbitdemonstratedthe obtainedfromrabbitlimbalexplantsandthenculturedonAlgi-Chi-FucmembraneorAM
highestpercentpermeabilityandrecovery.Itappearedthatincreasingtheconcentrationby for14days.Differentiatedandprogenitorimmunophenotypicpatternsofthecorneawere
oneorderofmagnitudeincreasedthepercentrecoveryinequine,whichmayindicatethat studiedwithanti-k3/k67andanti-p63antibodies.TheFeulgenreactionwasusedtodetect
tThheesedsipfefecrieenscmeaoyfbepneercfietnftrormecohvigerhyerbceotwnceeenntrtawtioonctooncreenactrhatmioanxsimwuams sncelgelriaglibpleer.mCeoabnicliltuy-. csohfrtowmaraetinfo/DrNimAa.geThanealsyasmisp(leImsawgeereJ(cid:1)e,vNaluIHate).dOupntdicearladnenospittyic(aOlDm)icarnosdcoinpteegcroautepdledopwticitahl
sions: Ahydrophilicsteroidanti-inflammatorydrugmodelwasabletopermeatethescle- density(IOD)relatedtothefunctionalstatesofchromatinandtheamountsofDNAwere
rprtANhaoeseoeurstinttsaeiretinean.anaisntttmhgaSneetenocatsrletteohletefraecnrrtdyeaerttdiiuonvgfeaslDpddedirefciuffsiuegeenssaisdsoeeeinsnlfioaivncerthrrvoyHiesstmseeaertelxhittnphehaeorsrAdycimfleoffaoerpianrrh.sttt.ThruaahInnltimdsslesoasrutuloiggAogggnwyeea.sasltrtSesdrudeapsNepapaoonorr.cstsheWiindbatl8enbe1rdysXuptsdWeheecevioHeeOfsl-otf1rpfid5amci-nfe1fsee-son0rcftel0ent3frhoc0aeerl. Lp((eqp2nluEe5ca<s.Cen1sgt8s0iri.fin0o(cid:1)ce0wudt0h1ln.t12euR)o..r0eenex8MdspuArevlMeotasssnn.s:i(o62IAnOL.32l.EgD5o0i%fC6-Cksv(cid:1)3ahvc/lsiuu1k-.eFl613ts7.uu.15crw2(e%pdemr,o=eoenpn0mh=.2AbAig1r0Mlhag)..ne0-,rCE1e)pxh.fpoaiT-<nrrFedh0suces.eci00loml0.msn50e4e1gaom)nrf(cid:1).obpOCwr60anoD3.n0ne1wocvnlaaaufsnlousAdtriewolsgAnociwM-s-eCf:elolrshsledMhi-go0Fgerw.ru5moee7cwabdtnr(cid:1)meanrneo0oeimn.nd0b1ifsfrraAfaofemnMomrer--
Alg-Chi-FucisaviablesubstrateforexpandingLECs.Thecellsgrownonthissubstrate
presentedmorecondensedchromatinandDNAthancellsexpandedonAM.Supportedby
ABSTRACT NO.: 016
theCNPq(Proc.300833/2010-5and467289/2014-0),andtheFAPESP(Proc.2012/17308-
Bilateral phacoemulsification followed by custom made 5and2013/01494-7).None.
intraocular lenses implant in howler monkeys (Alouatta
fusca and Alouatta caraya): case report
AKuner,*JAKleiner,†REFernandes*andRNudellman*
*VetService/Veteyes,RiodeJaneiro;†VetwebOftalmologiaVeterin(cid:3)aria,
Curitiba,Brazil
Purpose: Thiscasereportintendstodocumentcataractsurgeriesperformedin4howler
monkeys(AlouattafuscaandAlouattacaraya).Methods: Phacoemulsificationwasperformed
on4animals.Onepatientwasa4montholdAlouattafusca,male,orphan,rescuedindividual
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E34
E20 abstracts
ABSTRACT NO.: 019 coveredbyvessel,thevalueswere17.73%forgroup1,39.14%forgroup2,42.75%for
group3and30.66%forgroup4.Thereweredifferencesbetweengroup1andgroup3
Effects of the intracameral injection of preservative free (p<0.05).Conclusions: Allalkaliburnprotocolsexceptgroup1,inducedangiogenesisin
1% lidocaine, in the eyes of rabbits with experimental 100% of samples. Kinetics of induction and angiogenic growth seem to differ between
groups. Supported by the CAPES, the CNPq (Proc.300833/2010-5), and the FAPESP
uveitis (Proc.2009/51773-4).None.
AAFBarrosSobrinho,*TBLima,*KKKobashigawa,*RRenzo,* ABSTRACT NO.: 022
*IROMphtPhaadlmuoal,o*gyEUGnFitB,iDteelpia,†rtMmeAntldorfoVvaetneir*inaanrdyCJLlinLicaaulsM*edicineand Florida keratopathy in a red-bellied woodpecker –
Surgery,FacultyofAgrarianandVeterinarySciences,UNESPJaboticabal, a case report
SP,Brazil; †AnaethesiologyUnit,DepartmentofVeterinaryClinical ALazerson,*RLSwinger,*RRDubielzig,†LBTeixeira,†
‡ ‡
MedicineandSurgery,FacultyofAgrarianandVeterinarySciences, ALGardner andRMSchneider
UNESPJaboticabal,SP,Brazil *AnimalEyeGuys,FortLauderdale,FL,USA;†UniversityofWisconsin,
‡
Purpose: Toevaluatetheeffectsof1%lidocaineinpupildiameterandcornealendothelial SchoolofVeterinaryMedicine,Madison,WI,USA; SouthFlorida
parametersofrabbiteyeswithuveitis.Methods: Uveitiswasinducedbyparacentesisofthe WildlifeCenter,FortLauderdale,FL,USA
anteriorchamberinthelefteyeoftwentyrabbits.Twelvehourslatertenrabbitsreceived
0.2mLofpreservative-free1%lidocainethroughthesamemethod(treatedgroup–TG);the Purpose: TodescribeacaseofFloridakeratopathyinared-belliedwoodpecker(Melanerpes
othersreceived0.9%NaCl(nontreatedgroup–NTG).Thepupildiameter(PD)wasevalu- carolinus)andcomparethehistologicfindingswithothercasessubmittedtotheComparative
atedbypachymetryfor60min.Measurementsweremadeatoneminuteintervalsinthefirst Ocular Pathology Laboratory of Wisconsin (COPLOW).Methods: A wild red-bellied
tenminutes,fiveminutesfromthetenthtothethirtiethminuteandtenminutesfromthe woodpeckerwasbroughttotheSouthFloridaWildlifeCenterforaninjuredwing.Onoph-
thirtiethtothesixtiethminute.Endothelialcelldensity,coefficientofvariationandcorneal thalmicexaminationthelefteyehadmultiple,<2mm,round,focalareasofwhite,stromal,
thicknesswereevaluatedbynon-contactspecularmicroscopybeforeparacentesis(M0),12h cornealopacificationwithadensecenterandalessdefinedhalo,consistentwithFloridaker-
afterit(M1)andelapsed1h(M2),12h(M3)and24(M4)ofintracameralinjection.Re- atopathy.Nootherocularabnormalitieswerenoted.Theanimalwaseuthanizedforreasons
sults: NodifferenceswerefoundinPDbetweentheTGandtheNTG(p>0.05).The unrelatedtotheeye.Theeyeswereplacedin10%bufferedformalinandprocessedrou-
parametersinspecularmicroscopy,exceptcornealthicknessdidnotdifferbetweengroups. tinely.Theleftglobewassectionedinahorizontalplaneandbothhalvesweresubmittedfor
IntheTG,themeanvalueofcornealthicknessinM3(0.40(cid:1)0.03mm)wasdifferentfrom histology.Fivestepsectionsweremade,sampling10differentareasofthecorneawhich
M0(0.36(cid:1)0.01mm;p<0.05).IntheNTGaveragevaluesofcornealthicknessonM2 werestainedwithhematoxylinandeosin.Therightglobewasprocessedsimilarlyforcom-
(0.42(cid:1)0.07mm)andM3(0.41(cid:1)0.05mm)didnotdiffer(p>0.05),butdidsoinrelation parison.14casesofFloridakeratopathywereidentifiedintheCOPLOWcollection,com-
toM0(0.36(cid:1)0.01mm)andM4 (0.36(cid:1)0.03mm)(p<0.05).Conclusions: Whilethe prised of 8 dogs, 5 cats and 1 gray fox, and evaluated for comparison.Results: The
intracameralinjectionof1%lidocainedidnotmodifyPDandendothelialparameters,tran- corneal epithelium,stroma and endotheliumfrom both eyes were normal on histological
sientchangesoccurredin cornealthickness.SupportedbytheCAPES,theCNPq(Proc. examination.Nohistologicpathologywasfoundthatcouldexplainthecharacteristiccorneal
300833/2010-5and133228/2012-6),andtheFAPESP(Proc.2009/51773-4).None. opacities.ThisfindingwasconsistentwithallarchivedCOPLOWcasesofFloridakeratopa-
thy.Conclusion:Floridakeratopathyaffectsthered-belliedwoodpeckerandnoexplanatory
histopathologywasfound.None.
ABSTRACT NO.: 020
Intravitreal device for slow release of drugs to control ABSTRACT NO.: 023
inflammation during phacoemulsification Retrospective evaluation of feline granulomatous
TBLima,*IRMPadua,*KKKobashigawa,*MAldrovani,*FDY conjunctivitis
Claros-Chacaltana,*PESantos,*LCVieira,†AAFBarrosSobrinho,* ALazerson,*RRDubielzig,†LBTeixeira†andRLSwinger*
ASilvaCunha†andJLLaus* *AnimalEyeGuys,FortLauderdale,FL,USA;†UniversityofWisconsin
*OphthalmologyUnit,DepartmentofMedicineandSurgery,Facultyof SchoolofVeterinaryMedicine,Madison,WI,USA
AgrarianandVeterinarySciences,UNESPJaboticabal,SP,Brazil;
† Purpose: Tocharacterizetheclinicalappearanceandhistologicalfindingsoffelinegranu-
LaboratoryofPharmaceuticalTechnology,FacultyofPharmacyofthe lomatousconjunctivitis.Methods: Casesoffelineconjunctivitiswithhistiocytesasthepri-
FederalUniversityofMinasGerais,UFMGBeloHorizonte,MG,Brazil mary cell type and without a lipogranulomatous component were identified from the
ComparativeOcularPathologyLaboratoryofWisconsinrecordsbetween1993and2015.
Purpose: Toevaluatetheefficacyofaprototypebiodegradable(poly-lacticacidcopolymer Patientsignalment,history,treatment,andclinicaloutcomewereobtainedwhenpossible.
co-glycolic acid 50:50) slow-release device, containing dexamethasone, in the control of AllsampleswerestainedwithH&E,acidfast,GMSfungal,andgramstains.Results: 10
intraocularinflammationindogssubmittedtophacoemulsification.Methods: Fortyeyesof cases(6female,3male,1unspecified)werelocatedfrom9500felinesubmissions.3cats
20dogswithbilateralsenilecataractwerestudiedbydividingthemintotwogroups:CG,con- weremixedbreedand7werepurebreed.Averageagewas8.5 (cid:1)5.2years.Lesionlocation
trol;andTG,treatmentgroup.Allpatientswerepreparedforsurgeryaccordingtoestablished was bulbar conjunctiva (2), third eyelid (TEL) (6), or bulbar conjunctiva and TEL (2).
protocols. Immediately before initiating the interventions, the device was applied to TG LesionswereOD(5),OS(2)andOU(3).Thediseasepresentedclinicallyasadistinct,non-
patientsinthevitreous,viaparsplana.Postoperatively,therapeuticmanagementvariedbetween pigmented,raised,nodule(6),diffuseenlargementoftheTEL(1)orunspecifiedconjunctivi-
thegroups.CGpatientswereadministeredlocalandsystemicsteroids,antibioticsandcyclo- tis(3).Histologically,thesamplesconsistofsolidsheetsofgranulomatousinfiltratecom-
plegicdrops,asperthenorm.IntheTG,corticosteroideyedropsandsystemicdrugswerenot posed of bland epithelioid cells, often with distinct, vacuolated cytoplasm and occasional
administered.Patientswereevaluatedbybiomicroscopywithslitlamp,ultrasound,applanation multinucleatedgiantcells.Noinfectiousorganismswereseen.8caseshadconcurrentlym-
tonometryandlaserflaremetry,beforetheprocedureandatdifferenttimesaftertheprocedure. phoplasmacyticinflammation.1caseformednoduleswithnecroticandsuppurativecores,1
Differenceswereconsideredsignificantwhenp≤0.05.Results: Theoccurrenceofuveitis casehadneutrophilsinthesmallbloodvessels,and1casehadrareeosinophils.Followup
signswashigherinTG.Onultrasonography,therewasaslightreductionofthedeviceat wasunavailableformostcases.Twocaseshadnorecurrenceat8monthsand7yearspost
30days,andremainedthesameat60dayspostoperatively.Theintraocularpressure(IOP)was excision, respectively.Conclusions: Feline granulomatous conjunctivitis is a rare, idio-
higherintheTGrelativetotheCGandtheprocedureimmediatelyreducedtolowervaluesin pathicdisorder.Furtherstudyisneededtodeterminecauseandidealtherapy.None.
subsequenttimes.Therewas,inthegroup,theneedforsupplementaryanti-inflammatoryster-
oidtherapysitein35%ofcasesafterthefirstweekandby50%afterthethirdweekpostopera- ABSTRACT NO.: 024
tively. These showed flare higher than the control group, for 60days of
evaluation.Conclusions: Dexamethasoneslow-releasedevicesareemergingasanoptionfor Manual corneal thickness measurements of healthy
controllinginflammation.However,inthisstudy,theyofferednoadditionalbenefits.The
results obtained are preliminary and thus require further investigation. Supported by the caprine and camelid eyes using a portable spectral-
CAPES,theCNPq(Proc.300833/2010-5),andtheFAPESP(Proc.2009/51773-4).None.
domain optical coherence tomography (SD-OCT)
ABSTRACT NO.: 021 device
Angiogenesis models induced by alkali burns in a AJLoPinto,*CGPirie,*DBedenice*andSLAyres†
murine model *DepartmentofClinicalSciences,TuftsCummingsSchoolofVeterinary
†
FDYClaros-Chacaltana,*MAldrovani,*KKKobashigawa,*IRM Medicine(TCSVM); DepartmentofBiomedicalSciences,TCSVM
Padua,*GPValdetaro*andJLLaus* Purpose: ToestablishnormalcornealthicknessmeasurementsusingaportableSD-OCT
*OphthalmologyUnit,DepartmentofVeterinaryClinicalMedicineand dcaesviwceitihnohuetaoltchuylacrapdrisineaesaenwdecraemeevlaidlueayteeds.fMoretthhiosdstsu:dyC.lAinlilcaanlliymhaelasltwheyreadsueldtagtoedatsanadndgaelnptale-
Surgery,FacultyofAgrarianandVeterinarySciences,UNESPJaboticabal, manualrestraintwasusedtoensureproperpositioning.Cornealpachymetrywasperformedon
SP,Brazil etoyceoslowfaesacuhtialinziemdaulsiinngtritphleicOatpetboyvuae(cid:1)siniVglueeoSpDer-aOtoCrT(C(GOPp)t.oAvu6emInmc.cForreneemalonpat,chCyAm).etArlylpcroor--
Purpose: Evaluatetheeffectivenessofdifferentprotocolsofalkaliburnininducingcorneal nealmeasurementswereobtainedmanuallybyoneinvestigator(AJL)usingtheintegrated
angiogenesis in rats.Methods: Twenty four adult Wistar rats were stratified into four caliperfunction.Theepithelialthickness(ET),stromalthickness(ST),Descemet’smembrane
groups.Lefteyecorneaswerecauterizedbypressingafilterpaper(3mm)embeddedwith thickness(DT),andtotalcornealthickness(TCT)weremeasuredtwicefromeachimage.All
silverandpotassiumnitratesolution(3:1).Cauterizationtimewas10sforgroupIand20s recordedmeasurementswereanalyzedtodetermineintra-userreliabilityusingamixedeffects
forgroupII.Followingremovalofthefilterpapers,thecorneaswerewashedwithRinger model.Results: Eleven female goats with a mean ((cid:1)standard deviation; SD) age of
lactatesolution.CorneasofgroupIIIandgroupIValsowerecauterizedfor20sand10s, 6.4(cid:1)2.1yearswereexamined.MeanET,ST,DT,andTCT((cid:1)SD)was96.1(cid:1)5.0lm,
butthefilterpaperswerenotremovedbeforewashing.Anteriorsegmentphotosweretaken 486.0(cid:1)10.3lm,36.8(cid:1)4.8lm,and616.9(cid:1)7.1lm,respectively.Elevenalpacas(4male,7
usingaTRC-50DXretinacamera(TopconMedicalSystems,Inc.),followedby2,4,6,8, female)withameanage((cid:1)SD)of6.5(cid:1)3.6yearswereexamined.MeanET,ST,DT,and
11,13and15daysofcauterization.Thepercentageofcornealareacoveredbyvesselswas TCT was 147.4(cid:1)5.7lm, 446.1(cid:1)7.4lm, 44.5(cid:1)5.0lm, and 634.8(cid:1)6.2lm, respec-
estimatedafter15days,usingimageanalysissoftware(ImageJ(cid:1),NIH).Results: Twodays tively.Theintraclasscorrelationsforalllayersrangedfrom0.26to0.75.Conclusion: The
aftercauterization,33.3%ofcorneasingroupI,66.6%ingroupII,83,3%ingroupIIIand SD-OCTdeviceprovidesmanualcornealthicknessmeasurementswithclinicallyacceptable
100%ingroupIVshowedvascularization.Inthefollowingperiods,weobservedanincrease intra-operatorreliabilityinhealthycaprineandcamelideyesforET,ST,andTCTmeasure-
of corneal area covered by vessels in group 1, 2 and 3 (no difference between them, ments.Fundingsource:ThisstudywaspartiallyfundedbytheCummingsSchoolofVeterinary
p>0.05).Onthe15thdayofassessment,thecumulativeratesofcornealangiogenesiswere MedicineatTuftsUniversityCompanionAnimalFund.Disclosure:AJLoPinto(N),CGPirie
66.6%forgroup1and100%fortheothergroups.Regardingthepercentageofcornealarea (N),DBedenice(N),SLAyres(N).
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E34
abstracts E21
ABSTRACT NO.: 025 ABSTRACT NO.: 028
Intraocular pressure in dogs subjected to Intraocular pressure in Asian elephants by rebound
(cid:1)
pneumoperitoneum or to the Trendelenburg position tonometry (TonoVet )
KMCardoso,*LTNishimura,*DOGarcia,*MRDomiciano,* BCMartins,*RDWhitley,*PVillages*andRIsaza*
DPaulino-Jr,*EMattosJr,*PPMTeixeira,*MAMSilva,*AT *DepartmentofSmallandLargeAnimalClinicalSciences,Collegeof
Jorge,*FGGDias,*FVMamede†andCSHonsho* VeterinaryMedicine,UniversityofFlorida,Gainesville,FL,USA
*FrancaUniversity–UNIFRAN,Franca-SP,Brazil;†VeterinaryClinics Purpose: This study aimsto estimate the intraocular pressure (IOP) in Asian elephants
Oftalmovet–Ribeir~aoPreto-SP,Brazil (Elephasmaximus).Methods: Reboundtonometry(TonoVet(cid:1))wasperformedinbotheyes
ofeightAsianelephants.TheanimalswerehousedattheRinglingBros.andBarnum&Bai-
1P0urmpmosHe:gaTndhe10a(cid:3)imTroefndtheliesnsbtuurdgypwosaistiotoniansgs,eossntihnetrainocfluuleanrcpereosfsuCreO(2IOpPn)e.uMmoeptheroidtosn:euEmighotf lienyterCaecntitoenrafonrdEwleerpehmanatnCuaolnlyserrevsatrtiaoinnedatwCitehnoturatlthFelourisdeao.fAsleldaantiiomnalosrwaenreestuhseesdia.toNohutmopain-
adultBeaglesweresubmittedtotwosurgicalpositioningregimensatdifferenttimes.Ingroup calanestheticagentwasusedforthemeasurements.Results: Themean((cid:1)SD)IOPwas
GSI,theanimalsweresubmittedtodorsalrecumbencewithouthead-downtilt;ingroup 32.7 ((cid:1)0.55) mmHg and the median was 33mmHg when all eyes (OD and OS) were
TREN,thesameanimalsundergoneaTrendelenburgpositioning.Generalanesthesiawas included.Therewasnosignificantdifference(P=0.58)betweentheIOPvaluesoftheright
inducedusingpropofolandmaintainedwithisoflurane.IOP,meanarterialpressure(MAP), (32.73(cid:1)2.6) and left (32.07(cid:1)1.5) eyes. Collecting the IOP measurements was
centralvenouspressure(CVP),partialarterialpressureofCO2(PaCO2),heart(HR)and somewhat challenging considering that the animals could blink and remove the tonome-
respiratoryrate(RR),inthemoments:immediatelybeforeinsufflation(TIS);5(T5DI);30 ter probe from the instrument, and potentially lodge it on the lower conjunctival
(T30DI)and60(T60DI)minutesafterinsufflation.Thedataweresubmittedtoone-way fornix.Conclusions: This is the first report of intraocular pressure values in Asian ele-
ANOVAforrepeatedmeasures,followedbyBonferronitestformultiplecomparisonsamong phants.TheIOPvaluesinthisspeciesareamongthehighestknowninmammals.Thereis
timepoints,andttestforcomparisonsbetweengroups.Thesignificancelevelestablishedwas somerisktotheexaminerwhenperforminganophthalmicexaminationonanalertAsian
of5%.Results: ComparingGSIxTRENinisolatedmoments,onlythelefteyeshowedan elephant,andonlythosefamiliarwithtonometryandassistedbyanexperiencedelephant
increaseinIOP.TherewasadecreaseinMAPafterinsuflation.CVPdidnotchangeovertime handlershouldattemptit.None.
andbetweengroups.TherewasnodifferenceregardingHRbetweengroups.RRdiffered
bgreotwupees.nCGoSnIcalundsioTnRs:ENTihnedpifnfeeruemntoptiemrietopnoeiunmts.oTfhe10PamCmOH2,gshaonwdedthneo1d0i°ffeTrerenncdeebleentwbeuerng ABSTRACT NO.: 029
positiondidnotinfluencethePOandothercardiorespiratoryvariablesindogsanesthetized Comparison of intraocular pressure measurements
withisoflurane.SupportedbyPROSUP/CAPES.None. (cid:1)
using rebound (TonoVet ) and applanation (Tono-Pen
ABSTRACT NO.: 026 xl(cid:1)) tonometry in clinically normal alpacas (Vicugna
Development of an ex vivo equine corneal model pacos)
TLMarlo,*EAGiuliano,*ASharma*,†andRRMohan*,†,‡ JEMcDonald,*AMKnollinger,*DDDees†andNEMacLaren*
*DepartmentofVeterinaryMedicineandSurgery,CollegeofVeterinary *EyeCareforAnimals,SaltLakeCity,UT,USA;†EyeCareforAnimals,
†
Medicine,UniversityofMissouri,Columbia,Missouri; HarryS.Truman Austin,TX,USA
‡
MemorialVeteransHospital,Columbia,Missouri; MasonEyeInstitute,
Purpose: Toevaluatethemeanandrangesofintraocularpressure(IOP)estimatesineyes
SchoolofMedicine,UniversityofMissouri,Columbia,Missouri ofhealthyalpacas(Vicugn~apacos)usingrebound(TonoVet(cid:1))incomparisontoapplanation
Purpose: Todetermineiftheequinecorneaissuitableasanexvivomodel.Specifically,to (TonoPen-XL(cid:1)) tonometry.Methods: Complete ophthalmologic examinations were per-
acsusletussrethteecehqnuiiqnueesco(renitehae’sre(ax)traacnelaliurl/alriqmuiadtriinxtearnfdacceelolurla(bri)tyimafmteerrs7iodnayssysutseimng)ttowodedtiefrfemreinnet fmoermntesdusoinngfobrotythhTeaoltnhoyVaeltp(cid:1)acaansd(8T0oneoyPese)n.-SXcLhi(cid:1)rmtoenrotmeaertetresstwinegre(SoTbtTai)neadndOIUO.PDmateaaswuerree-
sr(ctpetAohciupanlleLlrieogtteruhCgbnarey(ec)eSl,s,ayiCtursRwemyeoRTixsntf,)thr-evePfmegisrtvnCrhhooodelmfRysooescrtqhahoeupn7aeanihrdlcntivdhorueeaaTtmsylochtaUsgoeto.edrraNraArnsnapfeeEldrlhlaoLliySSmsl,CamCampdRsRohlseeadoasmlcberyleaasel.o.derrinRnesMsgiaetuenrresvncpautahdttlhlhlaeieutotcdersaeedg:tcd(oesstae:duiS)invrnCegeuaErsiRterqhihon/’uueslncgiimqonp(sburhealena)inirdscceeitieoaamodillrelnnmoudetiegtenadehairirecsalfsbymaniwcoeaage(invsI,triaChoaolct.us)oro2sgaOtnlataopmdilnnoshietmtnloico,otcsusoconoslhgltrfeaunr(orpIdareofCpeea-rhl)mccisyoolytoia,rrrmsrrannthgknebeeiaemssaaand--lll ap2I1tOtooOn04Unn≤a..P82ool.y002mmz.T0emmeed5ttvhmmre,aeyrclursuo(cid:1)(cid:1)es,weniwnssew42igri(cid:1)dt..ea09heas10rS1tedDh2ammdo.e4ummss9rbiiegglOyb(cid:1)vnnOioiiarSfifiDu2e,ccn1p.,8aa4den1nar.22ttett.0moe1b.dnRdo0m(cid:1)-oiu0emfHmnfse2meudegr.a7letmtsen3Osurc:r(cid:1)tmDeheosaTmn,4v(Aop.hi1H1nmN2e3=gg.eO5Smta0O3TrV.ym10U(cid:1)TA.07.22Odv)Tmw.ae7Slshmeiu9,inregeHemn2sItO0gh(cid:1)ma.en8P(H108ds4.tvIg6a.mSOa29nlt0O–umPud2edaSms.re(cid:1)7o,dnm(cid:1)1b1t4dt’2mas(cid:1).Se.0i5vDnpm4i12aeaHi.dmtv5r(cid:1)iieog8madbn,2mae9.tpt7(-O5mwSpt8%eDUelsamet)n.nc,maowOwTtnHiteiDohhfitrnhgeee-,
apoptosisonTUNELassay,andincreasedaSMAonRT-PCR.Systema(ALC)maintained denceinterval(CI))increaseinIOPcomparedtotheapplanationtonometer.Nosignificant
parameters similar to normal equine controls with only minimal corneal edema noted differencesinIOPwerefoundbetweenODandOSwitheitherinstrument,age,gender,or
throughout the experiment.Conclusions: The ALC condition (a) maintains the equine breed.Conclusions: IOP readings from the rebound tonometer were statistically higher
cinornneeaar’sccoemllpulleatremdaetgrriaxdaantidonproefsenrovremsaclorenqeuailnetracnosrpnaeraelnacryc,hwitehcilteurteheaftIeCr7sysdtaeymsi(nb)cureltsuurlets. tshtuadniefsrocmomthpearaipnpglaTnaotnioonVetot(cid:1)noamnedteTr,ohnoowPeevne-rXnLo(cid:1)tcwointhsiddeirreedctclminaicnaollmyestirgincifimcaenastu.rFeumrtehnetrs
Theequinecorneacanbeusedasaviableex-vivomodelforfutureplannedstudiesprovid- maybenecessarytovalidatetonometryforroutineclinicaluseinalpacas.None.
inganappropriateculturesystemisused.SupportedbyVAFgrantVAF2015-03andfrom
theUniversityofMissouriPhiZetachapter.None ABSTRACT NO.: 030
Feline entropion: a retrospective study of 126 cats
ABSTRACT NO.: 027
(1995–2014)
Cataract removal and intraocular lens implantation in a
AMNicklinandAvanderWoerdt
Lanner falcon
TheAnimalMedicalCenter,NewYork,NY,USA
RMMarrion,*GAMessenger,†AFAlario,†FJRucker,‡IHofmann,§
DOMutti¶andCJMurphy** Preucropmomsee:ndTatoiondessicnri1b2e6thcaetssiwgnitahlmenentrto,ppiroens.enMtientghocodms:plaDinattsa,wclaisniccoalllescitgends,fraonmdtthreeatmmeedni-t
*BulgerVeterinaryHospital;†CapitalAreaVeterinaryEmergencyService calrecordof126catsdiagnosedwithentropion.Breed,age,sex,presentingcomplaints,clin-
andVeterinaryReferralCenterofNewHampshire;‡MassachusettsCollege icalsigns,andtherapywererecorded.Results: 126catswereincludedinthestudy.The
§ ¶ averageagewas7.8yearswitharangeof5weeksto18years.DomesticShorthairswere
ofOptometry; I-MEDPharma; CollegeofOptometry,TheOhioState mostcommon(76.2%)followedbyPersiancats(6.4%).Theremaining17.4%ofcatscom-
University;**CollegeofVeterinaryMedicine,UniversityofWisconsin- prised 12 other breeds. The most common presenting complaints were ocular discharge,
squinting,andcornealulceration.Entropionwaspresentbilaterallyin38%ofcatsanduni-
Madison
laterallyin62%ofcats.Thelowerlidalonewasaffectedin94.4%ofcats,theupperlidin
Purpose: To removecataracts in aLanner falcon andimplantcustom-made intraocular 3.2%andbothlidsin2.4%ofcases.50.7%ofeyeshadconcurrentcornealdisease(corneal
lenses.Methods: Afour-year-oldfemaleLannerfalcon(Falcobiarmicus)waspresentedwith ulceration,sequestrum,orperforation)atthetimeofdiagnosis.Moderatetomarkedble-
bilateralcataracts.Ophthalmicexaminationrevealedthepresenceofbilateralcataractswith pharospasmwaspresentin50%ofcats.Surgicalcorrectionofentropionwasrecommended
nootherocularpathology.Measurementsoftheanteriorocularcomponents,includingK inallcasesexceptforcatswhichwerepooranestheticcandidatesoriftheentropionwassus-
readingsandpupildiameter,weremadewithanoncontactocularbiometer(LenstarLS900: pectedtobespasticinnature.Ifsurgerywasdeclinedmedicalmanagementwithartificial
Haag-Streit AG, Konig, Switzerland), through a low-coherence interferometry technique. tearsandmanualrepositioningoftheeyelidswasrecommended.Surgicalcorrectionwasper-
BiometricmeasurementsrequiredtocalculateIOLpowerwereobtainedusingB-scanultra- formedin35.7%ofcatsexamined.Conclusions: Themeanageofcatswithentropionis
sound.Theresultswereusedtocalculateintraocularlenspowerandsize.Paraxialvergence greaterthanthatofdogswithentropion.Ocularsurfacediseaseiscommonincats with
equationsprovidedthelenspowerthatwouldmaketheretinaopticallyconsistentwithatar- entropion.None.
getrefractiveerrorof+0.50Dineacheye.Thelenswasassumedtobethinandlocatedat
theposterioraspectofthepre-surgicallensthickness.Ahydrophilicacrylicfoldablelenswas ABSTRACT NO.: 031
manufacturedbasedontheaboveinformation.CataractremovalwasroutinewithanAcrivet
phacoemulsificationsystemusedtoremovelenscontents,andtheartificiallensesinjected Associations of single-nucleotide polymorphisms of
intothecapsularbags.Results: Densecapsularopacitieswerenotedintraoperatively;these SRBD1 gene with glaucoma in Shiba-inu dog: a
precludedretinoscopypostoperatively.Thefalcon’svisionwasimprovedandshewasableto
perchandprehendfoodusingvision,whereasshereliedcompletelyontactilesensepriorto meta-analysis in six Japanese Veterinary Hospitals
sleunrsgeimryp.lTanotatthioenahuatvheorbse’eknnpoewrlfeodrgmee,dthinisaisfatlhcoenfi.rCstotnimcluescioatnasr:actInrtermaoocvuallaranlednsinimtrapolacnutlaar- MOta,*NKanemaki,†HTsujita,‡YKobayashi,§MAbe,¶
tion following cataract removal is feasible in falcons. P Lenses were manufactured by YTakimoto,**MImayasu,††AMeguro8andNMizuki8
I-MEDpharma. *AnimalEyeCenter;†AzabuUniversity;‡VeterinaryOphthalmology
§ ¶
SpecializedClinic; AliceAnimalClinic; AbeAnimalHospital;
**TakimotoAnimalClinic;††NagoyaCityUniversity;8YokohamaCity
University
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E34
E22 abstracts
Purpose: Weaimedtoperformameta-analysistoinvestigatetheassociationbetweenglau- Awithnoinfectionorfluorescenceindicativeofcompromisetotheepithelium.Histologi-
comapathologyinShiba-Inuandthesingle-nucleotidepolymorphisms(SNPs)ofglaucoma cally,amultilayeredepitheliumwasmaintainedandcornealedemawasnotappreciateduntil
candidategene,SRBD1.Methods: 152Shiba-Inudogswererecruitedfromsixveterinary day 14. All corneas with epithelial wounds healed with a healing rate of 0.655mm2/
hospitals in Japan. 97 Shiba-Inu dogs were diagnosed as glaucoma (IOP, more than h(cid:1)0.144mm2/handwerepositiveinIHCevaluationforsmoothmuscleactin.EMexami-
25mmHg) by tonometry. After purification of genomic DNA by buccal swabbing from nation shows epithelial layer confluence and migrating epithelial cells of normal cellular
glaucoma and normal dogs, three SNPs (rs8655283, rs22018514, rs22018513) in SRBD1 morphology.Conclusions: This model is an appropriate and clinically relevant tool for
gene, reported as a candidate gene allele for glaucoma in Shiba-Inu, were analyzed by assessmentandmodulationofepithelialwoundhealinginthecanine.None.
cycleavepolymerasechainreaction(c-PCR).Results: Ourmeta-analysisshowedthatthe
riskofglaucomainShiba-InucarryingTalleleofrs8655283was1.43timeshighercom- ABSTRACT NO.: 035
paredtothoseCallele(oddsratio(OR)=1.43,95%confidenceinterval(CI):0.85–2.41).
Carrying G allele of rs22018514 was 1.69 times higher than C allele (OR=1.69 (1.00– Changes in antibiotics resistance in equine bacterial
22..8059))t.imTehsehsitgrohnergersitskastshoacniatAionallwelieth(OglRau=co2m.0a9w(a1s.2f5o–u3n.d52i)n,GP=all0e.l0e0o80f3r)s.22C0o1n8c5l1u3sihoanvsi:ng ulcerative keratitis (2011–2014)
TheseresultsshowedthatthreeSNPsofSRBD1playanimportantroleinthepathologyof RVRamos,*CEPlummer,*BAButler*andKELamb†
gcalanucboemuaseidnaSshaibgae-nInetuicdtoegs.tinWgetoandteicteiprmatiendetfhoarttthheefiSrNstPtimgeenwotiythpipnrgecdiasitoanfrwohmetthheirsastduodgy *DepartmentofSmallAnimalClinicalSciences,CollegeofVeterinary
hasglaucomaandtopredictwhetheradogwilldevelopglaucoma.None. Medicine,UniversityofFlorida;†LambStatisticalConsulting,WestSaint
Paul,MN,USA
ABSTRACT NO.: 032
Purpose: To document changes in antibiotic resistance of organismsin cases of equine
Heat shock protein 70 expression in the equine cornea bacterialulcerativekeratitisoverfouryears.Methods: Medicalrecordsofequinepatients
CWPe*terson,*EADriskell,*DAWilkie,†CPremanandan†andRE wthiethyebaarcste2r0ia1l1u–l2c0e1r4atiwveerkeerreavtiiteiwsesdee.nTahtetrheesuUltnsiovefrmsitiycroofbiFallocruidltau’sreVaentderisnuasrcyepHtiboislpitiytaltefsotr-
Hamor ing fromthose caseswere examined. Isolated organismswere identifiedand subjected to
*UniversityofIllinois,CollegeofVeterinaryMedicine,Urbana-Champaign, Kirby-Bauerdiscdiffusionmethodsensitivitytest.Antimicrobialagentsusedinthesensitiv-
† itytestsincludedampicillin,bacitracin,chloramphenicol,cefazolin,ciprofloxaxin,enrofloxa-
IL,USA; TheOhioStateUniversity,CollegeofVeterinaryMedicine,
cin, gentamicin, neomycin, polymixin B, tobramycin and trimethoprim-sulfa.Results: A
Columbus,OH,USA totalof23bacterialisolatesweresubjectedtosensitivitytestingand11antibioticsevaluated.
Oftheseisolates,Streptococcusequisszooepidemicus,didnotdemonstrateresistanceforampi-
Purpose: Constitutive expression of the 70kDa heat shock protein (Hsp70) has been cillinandcefazolinineithertimeperiod,whilegentamicinindicatedoneinstanceofresis-
demonstrated in normal canine corneal epithelium and has been suggested to facilitate tancein2011–2012butnonein2013–2014.AsimilartrendwasobservedforPseudomonas
woundresolutionthroughorganizedmigration,proliferation,andadhesion.Tobroadenthe aeruginosa,butonlyforciprofloxacin.TherewerenooccurrencesofresistancetoStaphylococ-
understandingofcornealHsp70inothercompanionanimalspecies,expressionofHsp70 cusaureus Beta- lactamasefor ciprofloxacin and enrofloxacin observed in 2011–2012,how-
wasevaluatedinnormalandpathologicequinecorneastoestablishexpressionpatternsand ever,all antibiotics demonstrated resistance in 2013–2014.Conclusion: Streptococcus equi
todeterminewhetherHsp70mayserveasapotentialtherapeutictargetinequinecorneal subspecieszooepidemicus,PseudomonasaeruginosaandStaphylococcusaureuswerethemostcom-
woundhealing.Methods: Fixedtissuefromnormalequinecornea(n=11)andtherapeutic monorganismsisolatedfromcasesofequinebacterialkeratitisreferredtotheUniversityof
keratectomiesofsterilekeratopathies(n=9),weresubjecttoroutineimmunohistochemistry Floridafortheyears2011to2014.Thegeneraltrendwithinthepastfouryearssuggests
for Hsp70.Results: Normal equine corneas exhibited strong constitutive expression of thatStreptococcusequisszooepidemicusandPseudomonasaeruginosaarenotdemonstratingasig-
Hsp70 in the nuclei of basal and suprabasilar epithelial cells. Expression of Hsp70 in nificantincreaseinresistancefrom2011–2012to2013–2014whileStaphylococcusaureusBeta-
immune-mediatedkeratitiswasweaklyexpressedinthecytoplasmofbasalepithelialcells, lactamaseis.Whilenotstatisticallysignificant,primarilyduetosamplesize,inthemajority
whileexpressioninindolentulcerswasweakanddiffuseinthecytoplasmofallepithelial ofthecasesthereisadecreaseinorganismsusceptibility.None(nofinancialinterestor
cells,thoughstainingexpressionwasvariablebetweensamples.Conclusions: Thesefind- conflict).
ingssuggestexpressionofHsp70isinducedinthenormalequinecorneaandmaybealtered
insterilekeratopathies,possiblycontributingtotheirprolongedclinicalcourse.Additional
workisneededtobettercharacterizeexpressioninsterile keratopathies,especiallyinthe ABSTRACT NO.: 036
unaffectedepithelialtissue.SupportedbyUniversityofIllinois,CollegeofVeterinaryMedi- Basal cell carcinoma of the nictitating membrane
cineFacultyStart-up.None.
in a dog
ABSTRACT NO.: 033 RMRodriguezGalarza,*SMShrader,†JWKoehler,†ABeyer,*
Recurrent adnexal lymphoma in a horse PAMoore*andEAbarca*
BBMartabano,*DEBrooks,*RDWhitley,*LPProietto,* *DepartmentofClinicalSciences;†DepartmentofPathobiology,Collegeof
JAConway,†WMZoll†andCEPlummer* VeterinaryMedicine,AuburnUniversity,Auburn,AL,USA
*DepartmentofLargeAnimalClinicalSciences,UniversityofFlorida; Purpose: Todescribetheclinicalappearanceanddiagnosisofabasalcellcarcinomaofthe
†DepartmentofInfectiousDiseasesandPathology,UniversityofFlorida nictitatingmembraneinadog.Methods: A9-year-oldfemalespayedDachshundpresented
totheBaileySmallAnimalTeachingHospital,AuburnUniversityfollowinga2–3weekhis-
Purpose: Todescribeacaseofrecurrentadnexallymphomainahorse.Methods: A20- toryofoculardischargeintherighteye(OD).Ophthalmicexaminationrevealedanirregu-
year-oldQuarterHorsegeldingpresentedtotheophthalmologyserviceattheUniversityof lar, pink, multilobulated mass arising from the bulbar and medial aspect of the right
FloridaVeterinaryHospital(UFVH)forevaluationofasolitaryconjunctivalmassofthe nictitatingmembrane.Anincisionalbiopsyofmasswasperformed.Results: Histopathol-
righteye.Afocal,pink,fleshymassassociatedwiththedorsomedialbulbarconjunctivawas ogyfromanincisionalbiopsywasconsistentwithabasalcelltumor.Computedtomography
noted.Anexcisionalbiopsywasperformed.Results: HistopathologyrevealedaT-cellrich, oftheheadidentifiedawell-defined,softtissueattenuating,contrastenhancingovoidmass
B-celllymphoma(LSA)andcleanmargins.Theclientdeclinedstagingandfurthertherapy (arisingfromthenictitatingmembrane)inthecranioventralaspectoftherightorbit;the
attheinitialvisit.Approximately7yearslater,thehorsebegantoexhibitintermittentmild massdidnotinvadetheglobeororbit.Thenictitatingmembranewasresectedandcryother-
tomoderatediffuseswellingoftheconjunctivaoftherighteye.Sixmonthsafteronset,the apywasperformed.Basedoncellularmorphology,themasswasdiagnosedasabasalcell
swellingworsenedacutelyandwasaccompaniedbysevereswellingoftheleftconjunctiva. carcinoma.Conclusions: Totheauthors’knowledge,thisisthefirstcasereportofabasal
ThehorsethenreturnedtoUFVHandwasdiagnosedwithdiffuseconjunctivalT-cellLSA cellcarcinomainthenictitatingmembraneofadog.Althoughuncommon,basalcellcarci-
confirmedonimmunohistochemistryandwashumanelyeuthanized.Atnecropsy,neoplastic nomashouldbeconsideredinthedifferentialdiagnosesoftumorsofthenictitatingmem-
tissuewasobservedthroughouttheadnexalandoculartissuesofbotheyesandinthesub- brane.None
mandibularlymphnodes.Conclusion: OcularandadnexalLSAisanuncommonneoplasm
inthehorse.Totheauthorsknowledge,thisisthefirstdocumentationofanodularadnexal ABSTRACT NO.: 037
LSAthatinvolveda7-yearperiodwithoutrecurrencefollowedbydevelopmentofdiffuse (cid:1)
disease.Typically,surgicalexcisionofthenodularformofLSAinvolvingtheextraoculartis- Sutureless Acell xenograft and cyanoacrylate tissue
sues yields an improved prognosis compared to the diffuse form of the disease. Further
investigationisnecessarytodetermineifnodularformsofadnexalLSArepresentearlystages adhesive for corneal perforation in a pregnant mare
ofdiffuseextraocularorsystemicdisease.None. RMRodriguezGalarza,ABeyer,PAMoore,RCuming,MMora,
ABSTRACT NO.: 034 ESGroover,AAWooldridgeandEAbarca
DepartmentofClinicalSciences,CollegeofVeterinaryMedicine,Auburn
Development and assessment of a novel ex vivo canine
University,Auburn,AL,USA
model of corneal epithelial wound healing Purpose: TodescribetheuseofsuturelessAcell(cid:1)xenograftandcyanoacrylatetissueadhe-
LRProietto,*RDWhitley,*DEBrooks,*GESchultz,†DBGibson,† sive(CTA)inamarewithacornealperforation.Methods: An8-year-old,AmericanQuar-
MESalute,‡WMBerkowskiJr*andCEPlummer* terHorse,late-termpregnantmarepresentedtotheJTVaughanLargeAnimalTeaching
*SmallandLargeAnimalClinicalSciences,CollegeofVeterinary HOpohspthitaallm,AicuebxuarmninUantiiovnersrietvyeafolerde4vaclourantieoanluolfcemrsu:lttihprleeecmorinde-satlroumlcaelrattoiodnesepinstrtohmearilgchotrneyeael.
Medicine,UniversityofFlorida;†Obstetrics,Gynecology,WoundResearch ulcersandadescemetocele.Duetogeneralanesthesiarisktothefoal,ownerselectedmedi-
‡ caltherapyconsistingoftopicalantibiotics,antifungalsandmydriatics,andsystemicnon-
Center,CollegeofMedicine,UniversityofFlorida; SmallAnimalClinical steroidalanti-inflammatorydrugs.ASeideltestpositive,2mmcornealperforationatthe
Sciences,CollegeofVeterinaryMedicine,UniversityofFlorida descemetocele site with a shallow anterior chamber was noted the following day. The
peripheryofthecornealperforationwasdebridedanda2mmAcellxenograftwasposi-
Purpose: To develop an ex vivo model of canine corneal epithelial wound healing that tionedoverthefibrinclotandsealedwithCTA360°aroundthegraft.TheAcellxenograft
maintainscornealclarity,integrityandlimbalstemcellfunctioninextendedculture.Meth- and CTA corneal integration were evaluated using a 50MHz ultrasound biomicroscopy
ods: Corneoscleral(CS)rimswereexcised4mmposteriortothelimbusandplacedona (UBM)10and40dayspost-grafting.Results: Therewaspromptandpermanentrestora-
novelmutatingscaffoldinphysiologicconditions.InPartA,8corneasweremonitoredfor tionoftheanteriorchamber,withminimalinflammatoryresponsetothexenograft.The
epithelialintegrityandbacterialinfectionupto28daysinculture.InPartB,8corneaswere CTAremainedinplacefor17days.Fortydaysafterpresentation,UBMrevealedlossofthe
wounded,theepitheliumremovedandmonitoredforhealinginthesamecultureconditions CTAandincorporationoftheAcellxenograftintothestroma.Fourmonthsafterpresenta-
oraserumfreeproteinequivalentmedia.Photographsweretakenwithstandardizedscale tionthemareremainedvisualwithmildcornealfibrosisandaclearanteriorchamber.Con-
andcobaltfilterevery6hatthetimeoffluoresceinstainandmediachange.ImageJimag- clusions: The use of Acell(cid:1) xenograftand cyanoacrylatetissue adhesivecan bea viable
ingsoftwarewasusedtodetermineareaofpositivelystainingdefect.Healingratesofepithe- treatment option for poor equine anesthetic candidates with small corneal perforations.
lial wounds were calculated and CS rims then fixed for immunohistochemistry (IHC) or None
electronmicroscopy(EM).Results: AllcorneassurvivedtoendpointsasdescribedinPart
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E34
abstracts E23
ABSTRACT NO.: 038 ABSTRACT NO.: 041
Retinal imaging using optical coherence tomography Persistent hyperplastic primary vitreous and persistent
(OCT) in diabetic dogs hyperplastic tunica vasculosa lentis in three Great Dane
MBPBraga-S(cid:2)a,*PSMBarros,*PJDongo,*PFPacheco,* littermates
AABolzan*andAMVSafatle* KJSpahn,*LSWagner,*DDDees†andCMReilly‡
*SchoolofVeterinaryMedicine,SaoPauloUniversity,SaoPaulo,Brazil, *SouthTexasVeterinaryOphthalmology;†EyeCareforAnimals–Austin;
LaboratoryofExperimentalComparativeOphthalmology ‡VeterinaryMedicalTeachingHospital,UniversityofCalifornia–Davis
Purpose: Humanandexperimentalanimalstudieshaveshownapoptosisofneuralandglial Purpose: TodescribetheclinicopathologicfeaturesofthreeGreatDanelittermateswith
cellsintheretinaintheveryearlystagesofDR.Thepurposeofthisstudywastocompare presumedinheritedbilateralpersistenthyperplasticprimaryvitreousandpersistenthyper-
the retinal thickness (RT) measurements by OCT in diabetic dogs versus healthy plastictunicavasculosalentis(PHPV/PHTVL).Methods: ThreeGreatDanelittermates
dwdhooeaagpslshtp.haekyMrifcceootrdnhmotogerdsodsl(:nidno=S8gDs1d)-(,Oi5aobCffeeTtdmiicfaf(edlSeropsegenacstntr(bd4arle4feeDdmmos,aamll6eea–ssi1)n,a5n4Ody–eC1a45rTsmy,oeaaHlldreses()im,doelaedlpabnhe(mar=gkeica1En0dn.3=og)gi,6nsa.e9n(e)ndr,in=wcgoi7,tmh)CopauaAnrtd,eodUpctsuSoelAua9r-) ptsEioronnenusoecgwnlertaaaesptdehpdfyeorgrflwooerabvmsaelseupdfaretobriofmyonramtowfbeoodcladoroudingd-csyeawreltlyeiefirsepedaaetnxioedapnmdhtsietnhceraudelsmaimsnoegildcorgvtoiiossspctiooicbnpae.ilfcCoaralolenymeawspntlihdetetheasifHaotepErhwtmaihtnhyadldomruAiitalccsiiessaex.ndaUmaBtlilitnuoreaan--/.
disorders.Theselectionofanimalswasbasedonroutineandophthalmicexamination.OCT PASmethods.Results: Allthreepatientswereblindwithnegativemenaceresponses,non-
measurementwasperformedinthetemporalandnasalsuperiorretinausingamanualcali- responsive mydriatic pupils, and wandering nystagmus. All had mild-moderate ble-
peperit.hRelTiumwa.sUdnepfianiereddbty-ttehsetdwiastsaunsceedb(eptw<ee0n.0t5h)e.Rreetsinualltsn:erAvetfithbeertilmayeerofanOdCrTet,intahlepaivgemraegnet pfihbarorosisspaasnmd,pciognmjuenncttaitviaoln,hyapnedremmoiad,ereaptiesclbeurpalhtchoanlmgeisatioOnU,.diUffultsreasocournndeaslhoedweemda,cocmorpnleetael
timepostcataractsurgerywas11months,andmeandiabetesdurationwas19months.None bullousretinalseparationwithsubretinalfluid.Ahyperechoiclinearbandwaspresentfrom
showedchangesinIOPandweren’tbeingmedicatedatthetimeofexamination.TheRT theopticnervetotheposteriorpoleofthelensOU.Histologically,allglobeshadcomplete,
wheaasltdheycrceoasnetdrolisn d(1ia9b8e.0tic(cid:1)d1o6g.s7,lwmithvseirgsnuisfic2a2n0t.2th(cid:1)inn1i1n.g6lomf;thep=tot0a.l00r6e)t,inawictohmouptarevdisutaol ddiesgprleaecsedofreotrignaanlizseinpgarhateimono,rrsheavgeere,fiinbrtroavoacsuculalrarhteimssuoer,rhaangde,wainspdyctaotadraecnts.eAmllathriaxdavdajarycienngt
impairment.Conclusion: TheresultssupporttheconceptthatearlyDRmaybeassociated totheposteriorlens.ThematrixwasintenselyAlcianblue-positive,withregionsofvariable
with a reduction in retinal thickness. OCT allows measurement of retinal thickness and PAS-positivity;therewasvariableinvestmentbysmallvascularprofiles.Secondarylesions
monitoringofretinaldamageindogswithdiabetesmellitusthathavehadcataractsurgery includedneovascularglaucoma,chronicuveitis,andkeratitiswithstromalfibrosisandpig-
withexcellentreproducibility.Supported:FAPESPgrantsno2011/24039-8. mentation.Conclusions: ThisthefirstreportofPHPV/PHTVLintheGreatDane,sup-
ported by histopathology, and with evidence for a genetic basis in this litter. PHPV/
ABSTRACT NO.: 039 PHTVLshouldbeadifferentialforyoungGreatDaneswithblindness,cataracts,intraocular
hemorrhage,retinalseparation,orglaucoma.None.
Ductular squamous metaplasia of the gland of the third
eyelid in canines ABSTRACT NO.: 042
GCShaw,RRDubielzigandLBCTeixeira Primitive neuroectodermal tumor as a cause for
ComparativeOcularPathologyLaboratoryofWisconsin,Schoolof unilateral exophthalmos and bilateral blindness in a
VeterinaryMedicine,DepartmentofPathobiologicalSciences,Universityof
horse
Wisconsin
TDStrong,*KLTofflemire,*JSHaynes,*SAWiechert,*AN
dPougrsposies: aDsseotceiramteidnewifitdhuctpualartricsuqluaramosiugsnamlmeteanptl,asiaclionfictahlegsilgannsdoofrthehistthoirpdatheyoeloligdicianl Griggs,*RAAllbaugh*andGBenShlomo*
lesions.Methods: TheCOPLOWdatabasewasminedforcasesofcanineductularsqua- *LloydVeterinaryMedicalCenter,IowaStateUniversity
mousmetaplasiaoftheglandofthethirdeyelid.Caseswerereviewed,describedandsum-
marized.Results: Twenty-two cases of squamous metaplasia of the gland of the third Purpose: To report a case of unilateral exophthalmos and bilateral blindness due to a
eyelidindogswerefound.Affecteddogswereanaverageof11.8yearsoldandtherewere primitiveneuroectodermaltumorinahorse.Methods: Adonated15-year-oldThorough-
13malesand9females.Dachshundswereoverrepresented(7cases,32%).Themostcom- bred mare presented for progressive exophthalmos and blindness of the left eye (OS) of
monpresentingclinicalsignwasswellingormassofthethirdeyelid(21cases,95%).Histo- 7monthsduration,aswellasblindnessoftherighteye(OD)oftwomonthsduration.Phys-
logicallyaffectedductswerelinedbymultiplelayersofstratifiedsquamousepithelialcells, ical examination, including ophthalmic and neurologic evaluation, electroretinography
which occasionally occluded the lumens, with varying degrees of ectasia and intraductal (ERG), and necropsy were performed.Results: Clinical findings included mydriasis and
secretory material, sloughed epithelial cells and inflammatory cells (dochitis). Of the 22 absentmenaceresponsesinbotheyes(OU),aswellasresistancetoretropulsionandmild
cases,11wereassociatedwithadenocarcinomasoftheglandofthethirdeyelid,twowere conjunctivalhyperemiaOS.Additionally,therewasaunilaterallyprominentfacialveinon
associatedwithsquamouscellcarcinomapresumedtoarisefromtheductsoftheglandof theleftside.ThereweremultifocaldepigmentedareasinthenontapetalfundusOD,and
thethirdeyelidandtheotherninewereassociatedwithlymphoplasmacyticadenitis.Con- ERGresultswerenormalOU.Necropsyrevealedalarge,welldemarcated,redtobrown
clusions: Ductularsquamousmetaplasiaoftheglandofthethirdeyelidisassociatedwith massextendingfromthecribriformplatetothenasalcavityandthroughtheventralcalvar-
inflammation and neoplasiaof thethird eyelid andis frequentlyreported in dachshunds. iumandventromedialwalloftheleftorbit.Theopticchiasmandrightorbitweregrossly
None. normal.Histologically,bilateralaxonaldegenerationandgliosisoftheopticnerves,optic
chiasm,andoptictractswereidentified.Thetumorwasdiagnosedasaprimitiveneuroecto-
dermaltumorbasedonhistopathologyandimmunohistochemistry.Conclusions: Aprimi-
ABSTRACT NO.: 040
tive neuroectodermal tumor resulted in a unilateral retrobulbar mass and bilateral optic
Phenotypic characterization of corneal endothelial nerveaxonaldegeneration.Totheauthors’knowledge,thisisthefirstdescribedcaseofa
primitive neuroectodermal tumor causing exophthalmos and bilateral blindness due to
dystophy in German Shorthaired and Wirehaired involvementoftheopticpathwaysinahorse.None.
Pointers using in vivo advanced corneal imaging
ABSTRACT NO.: 043
ORShull,*SMThomasy,*LBDavis*andCJMurphy*,†
Assessment of the effects of eye drops on corneal
*DepartmentofSurgicalandRadiologicalSciences,SchoolofVeterinary
† wound healing with a novel primary epithelial cell
Medicine; DepartmentofOphthalmology&VisionScience,Schoolof
Medicine,UniversityofCalifornia,Davis,CA,USA toxicity assay
Purpose: German Shorthaired Pointers (GSHPs) and German Wirehaired Pointers HTakahashi,*,†KTajima,*THattori*andHGoto*
(GWHPs)mayhaveanincreasedprevalenceofcornealendothelialdystrophy(CED)butno *DepartmentofOphthalmology,TokyoMedicalUniversity,Tokyo,Japan;
studieshavecharacterizedthephenotypicchangesofCEDinthesebreeds.Thepurposeof †
thisstudywastofullycharacterizeCEDinGSHPsandGWHPswithnoninvasiveadvanced VeterinaryOphthalmologySpecializedClinic,Osaka,Japan
ocularimagingtechniques.Methods: Corneasof10affected(4GSHP,6GWHP)and19 Purpose: Wepreviouslydescribedanovelandsimpleprimaryepithelialcelltoxicityassay
unaffected(15GSHP,4GWHP)dogswereexaminedusingultrasonicpachymetry(USP), ofcornealexplantsthatassessestheeffectsofsingleandshort-durationinstillationsofeye
Fourier-domainopticalcoherencetomography(FD-OCT)andinvivoconfocalmicroscopy drops(Takahashietal.,Cornea2015;34:567–575).Thepurposeofthisstudywastousethis
(IVCM).Atwo-samplet-testorWilcoxonsignedranktestwereusedtostatisticallycompare assaytoevaluatetheeffectsofcommerciallyavailableeyedrops,whichhavereportedcorneal
1pa1gtv(rPiefa74ravfselr6.eeup<4aacl9eetym)tesc0e(rety(1.dPi0fte3vioe0nae5arr<1rlns8sya)ad–,0fbiff2.nfeer(05uePtec0cw2n3ts1tep9=aee)ddf)ee.dfo0cnecvItg.cenve0isttvlerc4hleesrswr)deuls/e.yiumsattdsshwCum(eoPonuodCg2anr.s=gEfnsafCtredfDer0ofacoioe.udl4tmcnaep)ttd.csenea.l3ndlouCd1Ddttsoe9hdoiagnsooittic(shtnagg2kreans:5analtli8irefiba1–eGslcces31oaipS56nndwrrH10gneeta)lesnPys(e1ac9ssnli0ve2dttal0ay6iletlnf6sh–idf/dde1iumca(ra9s6kGtsmi5e9nna8Wdm9t2eg)s–eiu1sHa1ndIs1n3ViiuP.cdna94sosCng5i6mn)w(M(4F6grpi2a.aDt9anhnUr(–wd-g4i1OCsSae9o35Ps)7EC.n.34–DT7Rs)7wtio3geaae(w7ns4sn1x)uii4hd9tfisll2hiticbc–mg1saoim5:n0tn,n9i.tem5firt1lAareyc)aossg(aurp6lenlkslre.etm9eeocalsdd–y-stf, epacblHrheesoyhiyisopyernaoaapnal(cid:1)tyuldrseoe.mayrragitooRnliirpnnvp(cid:1)aeueeaipms)xntwh.uepacieaTclnlhtads(tiesShinv:saHectipasotan)yppOnmw.drld(ioupTeMettapdrmogErelercaeAtetoocdohtaRwelrsodeteButodopharAfohscenL:tphdirhnha.A6eewtoTN-deTspewhplCrhwelefhirlmeEaafolfotso(cid:1)macervlru-lsyiaobl2aittngwubuehdmnflpriefenieieifiHtgnrphdcec.yeeaitdesarnlAhlhiilltarpefseleltaiyhsenenlc.eri(cid:1)uenriAenl)amaeylflcnteabtre(onocePerdtdoaxrhBrisirnoeceSnpricpwd)ertu-syeaa2tbsswr4waeaeosirteahtfsmivrthoa,aeeypentdeiaoTevpwfsrxecouiEcaat-orrihsmAfnner1edRaeteil2rnseiBoaeehfwllA0doys,.:ioeLl1t5l(suho0%Ap0t.wNwg1<lsar%SiC:Lot0HhwCEW.so0tio(cid:1)2fh5(rSdHc-)eT.muiawauylncI-ammaanhd8r-s
differencesincornealmorphologyincomparisontoage-matchedcontroldogs.Thesedata contrast,outgrowthratewassignificantlydecreasedwithHyalein(cid:1)comparedtoPBS-treated
betterinformthediagnosisandmanagementofCEDinthesebreeds.SupportedbyNIH controls(p<0.05).Inaddition,viablecellnumbershowedatendencyforsimilarresultsas
grantsK08EY021142,R01EY019970,andP30EY12576,ResearchtoPreventBlindness, theoutgrowthrate.Conclusions: Theprimaryepithelialcelltoxicityassayusingcorneal
UCDavisAcademicFederationInnovativeDevelopmentAward,andCenterforCompanion explantisapplicableforcornealwoundhealingassessmentsofeyedrops.Furtherstudiesare
AnimalHealth,SchoolofVeterinaryMedicine,UniversityofCalifornia,Davis.None. neededtodeterminewhetherthisassayisapplicableasamodelthatreflectswoundhealing.
None.
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E34
E24 abstracts
ORAL ABSTRACTS
ABSTRACT NO.: 044 ABSTRACT NO.: 047
Evaluation of the influence of estrogens in the lacrimal A retrospective analysis of environmental risk factors
production of female dogs in estrus or proestrus phases, for the diagnosis of deep stromal abscess in 390 horses
after ovariohysterectomy in north central Florida from 1991 to 2013
DYTrujillo,*JJRojas,*MYPadilla†andJJMesa* LRProietto,*CEPlummer,*KMMaxwell,*KELamb†andDE
*CollegeofVeterinaryMedicineandAnimalScience,Colombian Brooks*
CooperativeUniversity,Ibagu(cid:3)e,Colombia;†CollegeofVeterinaryMedicine, *DepartmentsofLargeAnimalandSmallAnimalClinicalSciences,
LaSalleUniversity,Bogot(cid:3)a,Colombia CollegeofVeterinaryMedicine,UniversityofFlorida;†LambConsulting,
Purpose: Evaluatetheinfluenceofthedeclineofestradiollevelsconsequenttoovaryohys- WestStPaul,MN,USA
terectomy(OHE)intearproductionincaninefemalesinestrogenicphases.Methods: 20 Purpose: Toidentifyenvironmentalriskfactorsforthediagnosisofequinedeepstromal
caninefemalesinproestrusorestrus,identifiedbyexfoliativevaginalcytologyandbymea- abscesses(DSA)inpatientswhopresentedtotheUniversityofFloridaVeterinaryMedical
surementofserumestradiol,weredividedintotwogroups:FemaleswhounderwentOHE Center (UFVMC).Methods: Cases included were selected from the UFVMC medical
(G1)andfemalesnotsubjecttoOHE(G2).Inbothgroups,tearproductionwasmeasured recordandimagingdatabase,andincludedallcasesofequineDSAdiagnosedduringthe
withSchirmerteartest(STT)atthreemoments.Day0,2and9:Before,twoandninedays period fromDecember1991 toDecember2013 in patients residingin subtropical north
aftertheOHEinG1;andday0,2and9aftertheonsetofproestrusorestrusinG2.STT central Florida. Atmospheric data were obtained for north central Florida for the corre-
valueswerecomparedwithineachgroupandbetween-groups.STTvaluesandserumestra- sponding date of diagnosis of each patient. Univariate and multivariate general linear
ddnaiioqylu0ewswewreeerreaen2ea3ml.y6pzel(oGdy1et)doafenovdral2sut1aa.tt8eismttihcmaeli/rmancinaolry(rGseils2at)(i;Poon<n.0Ad.a0Ny5O)2.V:R2Ae0s,au3nltd(sG:P1)eTaarhnseodna2v1Ce.r3oargmreemloa/tfimoSniTnTt(eGcoh2n-) mteiaoocnhdse.losRthewesrue,rletas:1g-emWneihlreae/tnhedyienactrer,setasiusnelgfuinrefdfwieoicnxtsidde(aPn(Sd=O02in).0tae0nr5ad)ctwsiioignnndsifiwcbaeenrtewtlyaeneinanlcyrezeenadvsieridnonttmhheeenpntrauelmsecbnoecnredooi-ff
adsseenifrrdfuiecmroeennsettdrsatatrdyiamido9eilowcploaowsrirna2ets1lsa.8t6ri8eom.vn8emapwl/geamd/smionnLbofsoeisnrrtavbtetihodsett,hicbGaugl1rtlyo,auswpnighse.nigliSeafitTciivanTentttvhyadepliuefGfee(sr2ceoonwrbcrtaeeaslsiant5(eiP9od.n6>i8ni0npt.d0hge5e/x)m.t<wLT0o.)h.SgeCTraooTvuneprcasalnugade-t tDtyhieoSeanrAfifwrcosairttsheiedsvyeebneryaytirfi1dea.e6dng3drseciegnanis°teirCfiso/cgyiaenennactrr.eadatWimsoexohiisdenpnehtte(ehNrmeicOpiern2ir)flsa,kuttuehfrnaeeccte(noPuormf=fotbe0rem.0Drp3Soe9Afr).actfCauosrroeemnswacdtlaiueoscsnrieoeviananslseut:dahteeWbdhyioin0nrds.1ec5sopi3nne4jeusdunpbeci-r-s
sions: Estrogens had no influence on the tear production of canine females, therefore, tropicalnorthcentralFlorida.Theimportanceofenvironmentaldynamicsintheincidence
OHEwasnotasurgicalprocedurethatpredisposedpatientstothepresentationofkerato- ofDSAindicatesthatthepathogenesisofDSAformationismultifactorialandinterdepen-
conjunctivitissicca.Support:Conadi.None. dentandfurthersupportsthemicropuncturehypothesisofDSAformation.None.
ABSTRACT NO.: 045 ABSTRACT NO.: 048
Schirmer Tear Test I values in normal horses with and Corneal fibrosarcoma in a cat
without auriculopalpebral nerve blocks RAAllbaugh,*SETangeman,*TSStrong*andJSHaynes†
HEVisser,*KADiehl,*RDWhitley†andKEMyrna* *DepartmentofVeterinaryClinicalSciences,IowaStateUniversityCollege
*DepartmentofSmallAnimalMedicineandSurgery,Collegeof ofVeterinaryMedicine,Ames,IA,USA;†DepartmentofVeterinary
VeterinaryMedicine,UniversityofGeorgia;†DepartmentofSmallAnimal Pathology,IowaStateUniversityCollegeofVeterinaryMedicine,Ames,
ClinicalSciences,CollegeofVeterinaryMedicine,UniversityofFlorida IA,USA
Purpose: TocompareSchirmerTearTestI(STTI)valuescollectedinhorseswithand Purpose: Todescribethefirstreportedcaseofcornealfibrosarcomainacat.Methods:
without anauriculopalpebral nerve block.Methods: STTI values were measured in 21 A12yearoldspayedfemaledomesticshorthaircatwithahistoryofsequestra3yearsprior
clinicallynormalresearchhorses(40eyes)withameanageof11yearsandamedianage wasexaminedforaslowlyenlargingpinkmassontheleftcornea.Examination,diagnostic
of12years.Theorderofeyestestedwasrandomized.Tearstripswereplacedinthecen- testing,transpalpebralenucleation,andrecheckvisitswereperformed.Results: Cytology
ter of the ventral fornix and values were recorded at 60s in mm of wetting/minute. oftheenlargingpinkleftcorneallesionatthetimeofpresentationwasnondiagnostic,while
Within24hto48h,atthesametimeofday,tearswerecollectedagainafteradministra- incisionalbiopsywasconsistentwithahighgradefibrosarcoma.Systemicevaluationdidnot
tion of an auriculopalpebral nerve block. Each block was preformed a minimum of five revealevidenceofmetastasis,andtranspalpebralenucleationwaselectedbytheowner.Ocu-
minutespriortoeachSTTI.Arepeatedmeasuresmodelwasusedtoanalyzedifferences larhistopathologyrevealedamasswithinthecentralcornealstromacomposedofinterweav-
betweenSTT Ivalues ineyeswithand withoutnerveblocksincorporatingwithinhorse ing bundles of anaplastic spindle cells and separated by fibrillar collagen. The neoplastic
correlation between eyes. The mixed model included fixed factors of treatment and eye cellshadfeaturessupportingahighgradefibrosarcoma(anisocytosis,anisokaryosis,multiple
and a random intercept for each horse. An unstructured covariance structure was nucleoli,mitoticrateof30per10highpowerfields).Thereweremultiplefociofnecrosis
used.Results: On average STT I values measured in eyes after auriculopalpebral nerve thatcontainedaggregatesofmineralwithinandadjacenttothemassaswellasfociofoss-
blocks were 0.55mm/min greater than those without nerve blocks. This difference was eousmetaplasia.Conclusions: Thisisthefirstknownreportofafelinecornealfibrosar-
notstatisticallysignificant(p=0.5268).Conclusions: Thereisnoeffectofauriculopalpe- coma.Developmentofthetumormayhavebeenassociatedwiththepreviouslydiagnosed
bralnerveblockonSTTIvaluesinhorses.SupportedbytheUGAOphthalmicResearch cornealsequestrum,asremnantsappearedstillpresentonocularhistopathology.Basedon
Fund.None. completesurgicalclearanceviaenucleationthepotentialformetastasisisexpectedtobelow
andlongtermprognosistobeatleastfair.Thepatientwillbefollowed,andmetronomic
ABSTRACT NO.: 046 therapyisbeingconsidered.None.
Endogenous cortisol concentration in canine tears and ABSTRACT NO.: 049
serum at rest and after a simulated stress event The histopathological results of Trabectome(cid:1) on the
BMWynne,*NANorton,†KADiehl‡andKEMyrna‡ iridocorneal angle in enucleated goniodysgenic canine
*CollegeofVeterinaryMedicine,UniversityofGeorgia;†Departmentof
‡ eyes
SmallAnimalMedicineandSurgery; DepartmentofLargeAnimal MDArmour*andLBTeixeira†
MedicineandSurgery *EyeCareforAnimals,Leesburg,VA,USA;†SchoolofVeterinary
Purpose: To evaluate levels of endogenous free cortisol in normal canine tears and
serumatrestandafterasimulatedstressevent.Methods: Pairedtearandserumsamples Medicine,UniversityofWisconsin,Madison,WI,USA
werecollectedfrom11normal,adultdogsoncedaily,atthesametimeofdayfor3con- Purpose: ToevaluatetheeffectsofTrabectome(cid:1)ontheiridocornealangleofgoniodys-
5sdeoclgugst/ikvogencIdeVa)ybseT.foePraearisraewndderteaetacro1laleancndtdeds2erbuhymppolsasactminipnglterasavgwelanesorseucsathpineiljnleacrtcyiooltnluebcwteeitdahtftArhoCemTmH4ed.ni(aoClrmocroatnlr,jousanydcnut(cid:1)il-t, fggooernniiacocducyatesngioennneisceetygelgsaluaduciorceomcmtal.ayFafoonlldlloobwwliiinnnggdnoeenwsusncewlreeacrteoionensv.eanMltu,aepttehadtoiedgnsot:nsiouCsnacdnoepirniwceaeplnlayttiraeonnudttsindwieahgeonnoupscreeledsaetnwiotietnhd.
vriacls(cid:1)forsnailxiv.aTryeacroarntidsolserEuLmISfAreevacloidrtaitseodlcfoonrceunsetraitnioncsanwineeretdeeatresrmanindedanusiInMgMaUSLalIiTmEet(cid:1)- oTfrabenecutcolmeaete(cid:1)dwaesyepse,rfoarnmdedthoenagldoebseigsnawteedresecsutibomniottfetdhefgoornihoidsytosgpeanthicoliorigdioccoarnnaelaylsiasngtloe
immunoassay respectively. Tear and serum cortisol concentrations were compared using COPLOW.Results: SectionstreatedwithTrabectome(cid:1)indicateanopenediridocorneal
moneea-ntaciloendcetn-tterasttsio.nRseosful0t.s5:7Clgo/rdtiLsoalndwa3s.1p3relsge/ndtLirnespreescttiinvgelyc.aTnihneectoenacresntarnadtiosneroufmcoar-t abnegculelacrhmareaschtewroizrekdsbpyacaen.iNncoretaissesuienrtehaectsipoancewoafstnhoetecdiliaarsyecxlpefetctaenddoenxpaannsieoxn-voivfothperotcrea--
t(apifst-oevlraliAuneCbT<o0Ht.h05st)teiamarnsudlaantwidoanssep(rprue-mvdaiclwutaeabsl<ys0i.gr0en5di)fiu.ccCaendotnlbyculetulseriveoamnteasd:ineoCdnoesritghisnooiufilrciasanfpttelryresAeelnCetvTaitnHedcsattnwiminouelhatoteiuaorrnss danugrele.iCnocnacselussoiofngso:nioTdryasbgeecntiocmgel(cid:1)aucmoamyabienedfofegcst.ivNeoantes.urgicallytargetingtheiridocorneal
andserumatrest.Cortisolconcentrationsincaninetearsandserumincreasesignificantly
followingstresssimulatedbyintravenousadministrationofACTH.Thepresenceofcorti-
solincaninetearshasthepotentialtoadverselyaffecttheoutcomeofcanineophthalmic
disease. Supported by the University of Georgia College of Veterinary Medicine Ocular
ResearchFund.None.
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E24
abstracts E25
ABSTRACT NO.: 050 ABSTRACT NO.: 053
Risk factors for sudden acquired retinal degeneration: The effect of trypan blue on posterior capsule
151 critically diagnosed cases within a reference opacification in an ex vivo canine model
population BMBrash,DAWilkie,AJGemensky-MetzlerandHLChandler
CRAuten,*SMThomasy†andDJMaggs† DepartmentofVeterinaryClinicalSciences,TheOhioStateUniversity
*CompanionAnimalHospital,MountProspect,IL,USA;†Departmentof Purpose: To determine if trypan blue (TB) reduces lens epithelial (LEC) or corneal
SurgicalandRadiologicalSciences,SchoolofVeterinaryMedicine, endothelialcellviability.Methods: Tissuewasharvestedfromcaninecadavers.Cultured
LECsweretreatedwithTBat0,0.05,0.1,0.2,or0.3%for30,60,or120s.Cellmorphol-
UniversityofCalifornia,Davis,CA,USA ogywasevaluatedandanLDHviabilityassayperformed.CulturedLECsweretreatedwith
0and0.3%TBfor120sandanapoptosisassaywasperformedtoassesscaspase-3activity.
Purpose: Todetermineriskfactorsforsuddenacquiredretinaldegeneration(SARD)diag-
ToevaluatetheeffectsofTBonexvivoPCO,followingmockcataractsurgery,lenscapsules
nosedbystrictcriteriawithinonereferralpopulation.Methods: Breed,age,gender,body
weretreatedwith0and0.3%TBattheabovetimesandmaintainedinculturefor2weeks.
weight(BW),andmonth/seasonofpresentationwerecomparedbetweendogswithERG-
Capsulesweremonitoredforchangesincelldensityandmorphology;histologywasper-
confirmed SARD and all dogs presented to the UC Davis Veterinary Medical Teaching
formedatexperimentalcompletion.Cornealendothelialcellsweretreatedwith0and0.3%
Hospitalfrom1991to2014.Results: SARDwasdiagnosedin151dogs(1.3%ofdogs
TB for 120s and an LDH viability assay performed.Results: TB did not significantly
presentedforophthalmicdisease).Althoughdogsof43purebreedswereaffected,thedachs-
hund(n=31),schnauzer(16),pug(11),Brittany(5),lhasaapso(4),Alaskanmalamute(2), reduceLECdensity.WhileTB-treatedLECsdemonstratehigherratesofcelldeathcom-
paredtovehiclecontrol,thedifferencewasnotsignificant.Inductionofapoptoticsignaling
beardedcollie(1),redbonehound(1)andsaluki(1)wereover-represented,andtheLabra-
wasfoundinTB-treatedLECcultures.ExvivoPCOformationwasnotsignificantlydiffer-
dor retriever (3) was under-represented in comparison to the reference population
(P<0.001).Median(range)ageofaffecteddogswas8.9(3–20)versus6.8(0.1–26)yearsfor entinanytreatmentgroup.EndothelialcellstreatedwithTBorvehicleshowednosignifi-
cant differences in cell death.Conclusions: TB induced low levels of LEC death via
the reference population. Middle-aged dogs were over-represented and young dogs were
under-representedintheSARDpopulation(P<0.001).Median(range)BWofaffecteddogs apoptoticsignalingcascadesbutwasnoteffectiveatreducingexvivoPCOformation.TB
was12.4(2.9–52.7)versus22.3(0.1–60)kgforthereferencepopulation.Dogsbetween10 did not induce endothelial cell death. Funded by ACVO Vision for Animals Foundation
and20kgwereover-representedintheSARDpopulation(P=0.006).Spayedfemales(59% grant(VAF2014-01).TrypanblueprovidedbyAcrivet.None.
ofaffecteddogs)wereover-representedcomparedtothereferencepopulationwhereasintact
females(1%ofaffecteddogs)wereunder-represented(P=0.002).Nosignificantdifference ABSTRACT NO.: 054
itnionmson(Pth=o0r.1seaasnodno0f.6p,rerseesnpteacttiiovnelyw).asCdoentecclutesdiobne:twSemenalltehre,SmAiRddDlea-angdedre,fesrpeanyceedpfoepmualale- Outcome of intravitreal gentamicin injections in 26
dogsareatanincreasedriskofdevelopingSARD.IncreasedriskofSARDinseveralbreeds glaucomatous canine eyes
suggestsafamilialfactorthatshouldbeinvestigatedusingmoleculargenetictechniques.
SupportedbyNIHgrantK08EY021142.None. BKBraus
DepartmentforCompanionAnimalsandHorses,UniversityofVeterinary
ABSTRACT NO.: 051 Medicine,Vienna;Austria
Blink patterns and kinematics of lid motion in normal Purpose: Toassessthelongtermoutcomeofintravitrealgentamicininjectiononglauco-
horses matous canine eyes.Methods: Following vitreal aspiration, 25mg of gentamicin was
injectedintravitreallyinto26blindcanineeyeswithprimary(21)orsecondary(5)glaucoma
LJBest,DVHHendrixandDAWard undergeneralanaesthesia.Thecauseofglaucoma,complicationsandfinaloutcomewere
CollegeofVeterinaryMedicine,UniversityofTennessee assessed.Failurewasdefinedastheneedtoenucleatetheoperatedeyeoreuthanasiaofthe
patientduetoocularpain;successwasdeterminedasacomfortableeyewithnoneedoffur-
Purpose: Todetermineblinkpatternsandestablishkinematicparametersoflidmotionin thertreatment.Results: MedianIOPbeforesurgerywas44.5mmHg(range8–72mmHg)
normalhorses.Methods: Tenadultmareswerefilmedfor10minusinghighresolution and10mmHg(range2–27mmHg)within6.5dayspostsurgery.Postoperativelyalleyes
videography.Onecamerawaspositionedperpendiculartotherighteyeandtheotherwas showedvaryingdegreesofcornealoedemaanduveitissuchaskeraticprecipitates,hyphaema,
positionedperpendiculartothelongaxisofthehorse.Fiveminutesofvideofootagewas aqueousflare,posteriorsynechiaandhyalitis.Failureoccurredin7/27(26%)eyesdueto
analyzedformeanblinkrate/min,numberofcompletevs.incompleteblinks,andnumberof incontrollableglaucoma,occurringwithinamedianof30days(range20–76days)following
unilateralvs.bilateralblinks.Videoofonerepresentativecompleteblinkfromeachhorse surgery.Nosignificantinfluencebetweenpriorandsecondaryglaucomawasevident.In20/
wasfurtheranalyzedwithimageanalysissoftwaretodeterminetheareaofcornealcoverage 27(74%)eyes,thesurgeryresultedinablindbutcomfortableeyewithnoneedoffurther
as a function of time during the blink.Results: The mean(cid:1)SD blink rate was medicationwithamedianfollow-upof632days(range12–2847days).Conclusions: In-
18.9(cid:1)5.5blinks/min. Blinks were subcategorized as complete (31(cid:1)13%), complete travitrealgentamicininjectionresultedinsignificantIOPreductioninallglaucomatouseyes
squeeze(6(cid:1)2%),minimalincomplete(30(cid:1)15%),andmoderateincomplete(33(cid:1)6%). post-surgery.However,alleyesshowedvaryingdegreesofuveitisfollowingtheprocedure
Blinks were unilateral 23% (OD: 12(cid:1)9%; OS: 11(cid:1)4%) of the time and bilateral andfailureleadingtoenucleationoreuthanasiaoccurredin26%ofthepatients.Support
77(cid:1)10%ofthetime.Themeanareaofexposedcorneaattheinitiationofeachblinkwas andConflictsofinterest: None.
5.89(cid:1)1.02cm2. Blink duration was 0.4667(cid:1)0.0712s. Lid closure was approximately
twiceasrapidaslidopening(0.16svs.0.31s).Conclusions: Themeanblinkrate/minin ABSTRACT NO.: 055
thehorsewassimilartotheblinkratereportedinnormalhumansubjects.Horseshadrela-
tivelyfewercompleteblinkscomparedwithnormalhumansubjects(suggestinggreatertear Targeting plasma membrane repair in corneal wound
filmstability)withclosurebeingmuchmorerapidthanopening.SupportedbytheUniver-
healing
sityofTennesseeCompanionAnimalFund.None.
HLChandler,RFWehrman,AJGemensky-Metzler,TTan,JMa
ABSTRACT NO.: 052 andHZhu
Complications following phacoemulsification in pugs: TheOhioStateUniversity
a retrospective study of 30 cases Purpose: ToevaluatetheprotectiverolethatMG53,anessentialgeneforcellmembrane
CBoss,1NCLaCroix,1DAWilkie,2FMaggio,3KTWiggans,4K rMepGa5ir3,pexlapyrsesisnioncoirnnehaulmwaonuncdornheeaallinepgi.thMeleiathlocdelsl:s(hWCeEstCer),nhbulmotasnwteeraers,usaenddtcoandineteeramquinee-
Kasier,5JSSapienza,6KKim,6RStrauss,6AvanderWoerdt7and oushumor.Membranerepairwasevaluatedusingmicroelectrodepenetrationingreenfluo-
GMSchmidt8 rescent protein (GFP)-MG53 expressing hCEC with subsequent observation of
translocation.Additionally,hCECunderwentglassbeaddamagefollowedbytreatmentwith
1TheVeterinaryMedicalCenterofLongIsland,WestIslip,NY,USA; recombinanthuman(rhMG53)andLDHanalysis.Theeffectoncornealwoundhealingwas
2TheOhioStateUniversity,Columbus,OH,USA;3TuftsV.E.T.S, evaluatedinaninvivoalkaline-inducedinjurymodelinrats.Results: HumanCEC,tears,
Walpole,MA,USA;4TheVeterinaryTeachingHospital,Universityof andcanineaqueoushumorallexpressedMG53,indicatingapotentialroleofMG53inpro-
tectingthecorneaagainstinjury.Indeed,livecellimagingrevealedthatmicroelectrodepene-
California,Davis,Davis,CA,USA;5AnimalEyeClinicofSpokane, tration could induce nucleation of GFP-MG53 to injury sites of hCEC. Additionally,
Spokane,WA,USA;6LongIslandVeterinarySpecialists,Plainview,NY, applicationofrhMG53significantlyprotectedhCECagainstmechanicalinjuryinducedby
USA;7AnimalMedicalCenter,NewYork,NY,USA;8AnimalEye imthicerlioa-lgizlaatsisonbe,aidnsd.icIantivnigvomcoemrnberaalnienjudraimesagdeemreopnasitrramteedditahtaetdrbhyMMG5G353copulladysimapnroimveproer-teapn-t
Clinic,Denmark,WI,USA roleincornealreparation.Conclusions: MG53enhanceshCECinjury-inducedmembrane
repairandplaysanimportantroleinimprovingcornealwoundhealingoutcomes.Thepres-
Purpose: Toreviewtheprevalenceofpreoperativeconfoundingdiagnoses,post-operative ence of MG53 in both the tear film and aqueous humor indicates that therapeutic
complicationsandvisualoutcomefollowingphacoemulsificationinPugs.Methods: Medi- approachesinvolvingexogenousMG53areunlikelytoinvokeinflammatoryresponsesinthe
calrecordsof54eyesof30pugsthatunderwentphacoemulsificationatsevenclinicalcenters eye.
were reviewed. All cases were monitored for a minimum of 3months following cataract
surgery.Results: Median(range)ageatpresentationwas8.5(4.0–12.7)years,with20neu- ABSTRACT NO.: 056
teredmales,2intactmales,and8spayedfemalesrepresentedinthestudy.Preoperativefind-
ingsincludedpigmentarykeratitis(22/30dogs),diabetes(21/30dogs),intraocularpressures Cytokine profiles of aqueous humor and serum in
(IOP) <10mmHg (23/54 eyes), corneal ulcerations (2/54 eyes), and Schirmer’s tear test
(STT)values<10mm/min(51/54eyes).Atthetimeofsurgery,cataractswereclassifiedas horses with uveitis measured using multiplex bead
immature(10),mature(28)orhypermature(16).Prostheticlenseswereimplantedin52/54
eyes.Within3monthsfollowingphacoemulsification,13.0%(7/54)ofeyesdevelopedcor- immunoassay analysis
nealulceration,7.4%(4/54)experiencedIOP>30mmHg,5.6%(3/54)hadretinaldetach- ECurto,KMMessenger,BASalmonandBCGilger
ments,and1.9%(1/54)hadSTTvalues<10mm/min.Median(range)postoperativefollow-
upwas18.0(3.5–60)monthsatwhichtime20.4%ofeyes(11/54eyes)wereblindin23.3% CollegeofVeterinaryMedicine,NorthCarolinaStateUniversity.
(7/30)ofpugs.Conclusions: Althoughareferencepopulationwasnotusedhere,compar- Purpose: TodeterminewhetherhorseswithclinicallydiagnosedEquineRecurrentUveitis
isonswithpreviouslypublisheddataforavarietyofbreedssuggestthatpugsmaybeatan (ERU)haveaspecificcytokineprofileintheiraqueoushumor(AH)andserumthatdiffers
increased risk of complications following phacoemulsification; notably retinal detachment fromhorseswithuveitissecondarytootherocularinflammatoryprocessesandfromhorses
leadingtoblindnessin6/11eyes.Studiesincludingareferencepopulationareneededtoval- withnormaleyes.Methods: SamplesofAHandserumwereobtainedfromhorseswith
idatethesefindings.None. ERU,uveitissecondarytootherinflammatoryoculardisease,non-recurrentuveitis(UV),
andnormaleyes(N)presentingtoNorthCarolinaStateUniversity.Sampleswereanalyzed
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E24
E26 abstracts
forexpressionofcytokinesusingamultiplexbeadimmunoassay.Results: InAHofhorses stromalloss,andexuberantgranulationtissueformationwarrantingfurthertreatment.Inthe
withERU,meancytokineconcentrationsofEotaxin,G-CSF,IL-1a,IL-10,MIP-2,IP-10, corticosteroid treatment group, 7.7% of cases had a complication. Complications in this
MCP-1,andRANTESwere976,30,11,10,54,65,84,and1132pg/ml,respectively.In groupdidnotincludeinfection,infiltrateorstromalloss,andwereisolatedtopersistentvas-
horseswithinfectiouskeratitis(IK),meanAHcytokineconcentrationsofEGF,IL-1a,IL-4, cularization(n=1)andshort-termrecurrenceofulceration(n=2).Anecdotally,corticos-
IL-6,MIP-2,IP-10,andMCP-1were6,13,4,2903,62,68,and167pg/ml,respectively.In teroid-treated dogs experienced less irritation after interventions such as
horseswithUV,meanAHcytokineconcentrationsofIL-1a,IL-10,MIP-2,andIP-10were debridement.Conclusions: Theadditionoftopicalcorticosteroidtherapyinthetreatment
18,85,52,and41pg/ml,respectively.NocytokinesweredetectedinAHfromnormaleyes. ofvascularizedcanineSCCEDdoesnotresultinlongerhealingtimesnorincreasedcompli-
HorseswithERUandIKhadelevatedserumlevelsofIL-6,EGF,IL-18,IFN-c,IP-10and cationratesorseverity.None.
MCP-1comparedUVandNgroups.SerumeotaxinandIL-10wereelevatedinhorseswith
IKcomparedtoallothergroups.Conclusions: Apro-inflammatorycytokineprofilesup- ABSTRACT NO.: 060
portiveofmononuclearcellchemotaxiswasdetectedinAHsamplesofhorseswithuveitis.
EotaxinandRANTESinAHmaybespecificforERU.None. The effects of topical diclofenac and flurbiprofen on
corneal sensitivity in normal dogs
ABSTRACT NO.: 057
DMDorbandt,ALLabelle,MAMitchellandREHamor
Preliminary clinical findings in treating canine
DepartmentofVeterinaryClinicalMedicine,CollegeofVeterinary
keratoconjunctivitis sicca with 1% tacrolimus aqueous
Medicine,UniversityofIllinois
solution
Purpose: Evaluatetheeffectsof0.1%diclofenacand0.03%flurbiprofenoncornealsensi-
TMCzepiel,JSSapienzaandRSStrauss tivityinnormalcanineeyesafterrepeateddosingand30daysoftwicedailydosing.Meth-
ods: Aprospective,randomized,masked,crossoverstudyof18healthy,non-brachycephalic
LongIslandVeterinarySpecialists,Plainview,NY,USA dogswasperformed.ForPhaseI,thestudydrugwasinstilledevery5minforfivedoses,
Purpose: Toevaluatetheeffectoftopical1%tacrolimusaqueoussolutionontearproduc- andcornealsensitivityofthecontrolandtreatedeyewasevaluatedpriortodruginstillation
tionindogspreviouslydiagnosedwithkeratoconjunctivitissicca(KCS)refractorytoother andevery 15min post-instillationfor 60min. ForPhase II,the studydrugwasinstilled
lacrimostimulants.Methods: MedicalrecordsofdogsevaluatedatLongIslandVeterinary every12hfor30days,andcornealsensitivityforthecontrolandtreatedeyewasevaluated
SpecialistsfromAugust2014toJanuary2015werereviewed.Inclusioncriteriaconsistedof priortodruginstillationondays0and30.Awashoutperiodofatleast30daysoccurred
fdtmriomoegmdesisctfadroteauiaoirltntyeesde.wnwTitdihrtoehgarets1m-%ewevenatrltaeucawertoliiitolghinmib1ul2e%s–5ftohtwraacteinredockellsiummsaoifuotnsensr.twrPiaanrtsieitodiparrtaeitoosScnrTtihobTefedstothuftedwr<yao7,pyam(.nllmR=e/eym6esu)sinlwttosde:erteshpTrtiertweeeeaptn(enrtdye=vweio1yiute4hss) mbt2h5eurt.o1wltuie(cid:1)pgelhne6o.uid8ntrskutdgigla,latcrateriooscpnsosesolclvtoeiecfvrteidloyaincn.l.doTRfehbeneesartuecwltew(sPee:nr=eAp0nvh.oe6ars7ase)igsg.eonrTiafiflgecuemarnpbateinpdrdarifotfufweerreeneinga(chPnetds=wihn0eu.r5cme4o)ird.5ni.eC7tayol(cid:1)rwsne3een.ra3selitysimveeinaetrsyasistauivafrtnieetddyr
w0o.ii0nt2htm%ouetntaticm(rnopl=rimo5vue)s,m2seo%nltutciiyoncnl,oSsaTpnTodr.i1n7Be/e2sf0oorlueetyieotshnear(lasnpoy=re9wc)e,itihvoerd10%t.2re5a%ttamcrpeonillitomcwauirstp,hinS0eT.2sT%olurcteyisocunloltss(pnow=reinr8ee) w(cPoars=nea0al.sl8o2s)ennoosrtitflisviugirtnybiifipmcraoenfaetslnuyr(ePdmif=efen0rte.s5n6t()P.afD=tee0rc.r03e00a4s)di.nagCyshounomfcliutdwsiiitocyenwsd:aasilNaysesuoitscheieartwedidthiwclioetihftehnaenarcidnniccorleroafseflenuairnc-
≤7mm/minin20/20eyesand≤2mm/minin10/20eyes.Atfirstre-evaluation,tearproduc- biprofenhaveaneffectoncornealsensitivityinnormalcanineeyesafterrepeateddosingor
tion improved by ≥3mm/min in 60% of cases (n=12). Of those 12 eyes, 50% (n=6) 30daysoftwicedailydosing.Ambienthumiditymayhaveaneffectoncornealsensitivity
demonstrated a STT increase of ≥10mm/min. For the 10 eyes with an initial STT of measurementswithalongerfilamentlengthelicitingablinkresponseatalowerhumidity.
≤2mm/min,70%(n=7)experiencedaSTTimprovementof≥5mm/min.Oftheeighteyes None.
withunchangedSTTatthefirstre-evaluation,twodemonstratedimprovedkeratitis.Con-
clusions: Administrationoftopical1%tacrolimusaqueoussolutionisapromisingtreat- ABSTRACT NO.: 061
mentfordogswithrefractoryKCS.None.
3D printing for orbital and peri-orbital masses
ABSTRACT NO.: 058 DMDorbandt,SJoslynandREHamor
Globe sparing procedure for canine iridociliary tumors DepartmentofVeterinaryClinicalMedicine,CollegeofVeterinary
Medicine,UniversityofIllinois
via surgical debulking with adjunctive endoscopically
Purpose: Describe the technique and utility of 3D printing for orbital and peri-orbital
guided transscleral cryotherapy with or without diode masses.Methods: Computedtomography(CT)andsubsequent3Dprintingofthehead
endolaser photocoagulation wasperformedontwodogs.Caseonewasan11-yearoldspayedfemaleDachshundwitha
3weekhistoryofnon-painfulexophthalmosoftherighteye(OD).Casetwowasa13.5-year
TMCzepielandJSSapienza oldcastratedmaleEnglishSpringerSpanielwitha5weekhistoryofnon-painfulexophthal-
LongIslandVeterinarySpecialists,Plainview,NY,USA mosOD. Abiopsyof themassobservedwithCTwasobtainedin bothcases.Alateral
orbitotomywastentativelyplannedforeachcase,anda3Dprintofeachheadwiththeasso-
Purpose: Todescribeanovelglobesparingprocedureforcanineiridociliarymassesvia ciatedglobeandmasswascreatedtoassistinsurgicalplanning.Results: BothCTscans
surgical debulking and adjunctive endoscopically guided transscleral cryotherapy with or confirmedaconfinedmass.Incaseone,themasswaslocatedinthecranioventralaspectof
without diode endolaser photocoagulation.Methods: Medical records of dogs receiving therightorbit,andthehistopathologicdiagnosiswasadenoma.Duetosuspicionsofanicti-
treatmentforciliarybodytumorswithsurgicaldebulkingofthemassinadditiontoadjunc- tansglandadenomabasedonlocation,thenictitansandassociatedtumorwereremovedwith
tivetransscleralcryotherapyplusorminusendolaseratLongIslandVeterinarySpecialists completeexcision.Incasetwo,themasswaslocatedwithinthelateralmassetermuscleven-
from October 2012 to April 2015 were reviewed.Results: Three eyes from three dogs tral to the right orbit, between the zygomatic arch and ramus of the mandible. The
wereeligibleforinclusion.Preoperatively,incipientcataract(n=2)andhypotony(6mmHg; histopathologic diagnosis was consistent with a lipoma, and due to the animal’s age and
n=1) were noted. Surgical debulking with adjunctive endoscopically guided cryotherapy benignnatureofthetumor,continuedmonitoringwaselected.Conclusions: 3Dprinting
(n=3) and endolaser (n=2) was performed. Histopathology confirmed an iridociliary incaseswithorbitalandperi-orbitalmasseshasexceptionalpotentialforimprovedsurgical
epithelialtumor(n=1),iridociliaryadenoma(n=1),andiridociliarytumor(n=1).Long- planning.Additionally,3Dprintingprovidesanothermodalityforvisualizationtohelpvet-
termmedicalmanagementconsistedoftopicalanti-inflammatorymedications.Postoperative erinarians,students,andclientsunderstanddistributionofdisease.None.
complicationsincludedeyelidmargindepigmentation(n=2),superficialcornealulceration
(n=1), corneal edema (n=1), and anterior uveitis (n=3). Permanent ocular changes ABSTRACT NO.: 062
included focal posterior synechiae (n=3), focal anterior synechiae (n=1), and corneal
degeneration(n=1).Mild,non-visionthreateningcataractprogressionwasnoted9months Treatment of an orbital lacrimal gland adenocarcinoma
postoperativelyinonecase.Alleyesmaintainedvisionwithnoevidenceoftumorrecurrence
duringthefollow-upperiod(9–30months).Conclusions: Surgicaldebulkingofcanineiri- in a cat
dociliarymasseswithadjunctiveendoscopicallyguidedtransscleralcryotherapyplusorminus DMDorbandt,*APLundberg,*PJRoady†andREHamor*
diodeendolaserphotocoagulationisaviabletherapeuticoptiontopreservevision.None. *DepartmentofVeterinaryClinicalMedicine,CollegeofVeterinary
†
ABSTRACT NO.: 059 Medicine,UniversityofIllinois; VeterinaryDiagnosticLaboratory,College
ofVeterinaryMedicine,UniversityofIllinois
The use of topical corticosteroids in the management of
Purpose: Todiscussthediagnosisandtreatmentofanorbitallacrimalglandadenocarci-
vascularized canine spontaneous chronic corneal nomainacat.Methods
epithelial defects (SCCED) A14.5-yearoldcastratedmaledomesticshorthaircatwasevaluatedfora2weekhistoryofa
swollenleftuppereyelidthatwasunresponsivetosystemicantibiotictherapy.Uponexami-
KADiehl nation,adorsalorbitalmasswasidentifiedandnotedtomoveindependentoftheleftglobe
DepartmentofSmallAnimalMedicineandSurgery,CollegeofVeterinary (OS).Mildventralstrabismuswaspresent,butnootherabnormalitieswerenotedOS.The
masswassurgicallyexcisedandsubmittedforhistopathology.Adjunctivecryotherapywas
Medicine,UniversityofGeorgia performedatthesurgicalwound.Post-operativetreatmentconsistedofneo/poly/bacthree
timesdailyOSfor1weekand6mgofrobenacoxibbymouthoncedailyfor2days.Re-
Purpose: Todetermineiftheuseoftopicalcorticosteroidanti-inflammatorymedicationin
sults: Histopathologyoftheexcisedmassconfirmedanadenocarcinoma,andspecialstain-
vascularized canine SCCED impacts healing rate, or frequency or type of complica-
ingusingmucicarmineandperiodicacidSchiffconfirmedaserousglandorigin.Priorto
tions.Methods: Recordsfromtwopracticesovera6-yearperiodwerereviewedtoidentify
surgery,nometastaseswerenotedviathoracicradiographs,andafullbodyCTscanper-
primarySCCEDcaseswithnotablecornealvascularizationandfollowupinformation.Cases
formed5weekspost-surgerydidnotrevealanymetastaticdisease.Atafollow-upevaluation
were excluded if they had a keratectomy; debridement, anterior stromal puncture and/or
5weeks post-surgery, the surgical wound was healed with no evidence of tumor
diamond burring was permitted. Time to healing between non-corticosteroid and
regrowth.Conclusions: To the authors’ knowledge, this is the first reported case of a
corticosteroidtreatmentgroupswascomparedviatwo-tailed,non-pairedstudentttest(sig-
nificanceP<0.05).Frequencyandtypeofcomplicationswerenotedforbothgroups.Re- felineorbitallacrimalglandadenocarcinoma,anditshouldbeincludedasadifferentialdiag-
nosisformasseslocatedinthefelinedorsalorbit.Surgicalexcisionwithadjunctivecryother-
sults: Fifty-sixcasesin49dogswereidentified,and40ofthosecasesweretreatedwitha
apymaybeaneffectivetreatmentstrategyifconfinedtotheanterioraspectoftheorbit.
topicalcorticosteroid.Meantimetohealinginthenon-corticosteroidtreatmentgroupwas
None.
26.5daysversus23.1daysinthecorticosteroidtreatmentgroup.Thisdifferencewasnot
statisticallysignificant(P=0.48).Seriouscomplicationswererare.Thenon-corticosteroid
treatmentgrouphadcomplicationsin35.3%ofcases;includinginfectionandinfiltratewith
©2015AmericanCollegeofVeterinaryOphthalmologists,VeterinaryOphthalmology,18,E17–E24