3D Analysis of the Myocardial Microstructure: Determination of Fiber and Sheet Orientations

86 Pages·2016·7.467 MB·English

by  Johanna Maria Ticar (auth.)

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Johanna Maria Ticar
3D Analysis of the
Myocardial Microstructure
Determination of Fiber
and Sheet Orientations
Johanna Maria Ticar
Graz, Austria
OnlinePlus material to this book can be available on
http://www.springer-spektrum.de/978-3-658-11423-7
BestMasters
ISBN 978-3-658-11423-7 ISBN 978-3-658-11424-4 (eBook)
DOI 10.1007/978-3-658-11424-4
Library of Congress Control Number: 2015950752
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Acknowledgment
ThismasterthesishasbeencarriedoutattheInstituteofBiomechanicsat
Graz,UniversityofTechnology. Iwouldliketoexpressmygratitudetoall
peoplewhosupportedme. Specialthanksaremainlyduetomysupervisor
Gerhard Sommer, PhD, for his guidance, contribution of knowledge and
experience,supportandvaluablecommentsandtotheheadoftheInstituteof
BiomechanicsProf. GerhardHolzapfel,PhD.Iwouldalsoliketoconveymy
gratitudetoAndreasSchriefl,PhD,forhiseffortandvaluablehelpduring
thedataanalysis,andHeimoWolinski,PhD,forhisexpertiseconcerning
microscopy. Moreover,IamthankfultoDipl.-Ing. ClemensDiwoky,who
enabledperformingthefeasibilitystudyusingDTMRI.
LastbutnottheleastimportantIowemythankstomyfamilyandfriends
whosupportedandencouragedmethroughoutmyyearsatuniversity.
Contents
ListofFigures IX
Abstract XIII
Kurzfassung XV
1 Introduction 1
2 MaterialsandMethods 7
2.1 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Diffusiontensorimaging . . . . . . . . . . . . . . 9
2.2.2 Multi-photonmicroscopy. . . . . . . . . . . . . . 12
3 Results 29
3.1 Diffusiontensorimaging . . . . . . . . . . . . . . . . . . 29
3.2 Multi-photonmicroscopy . . . . . . . . . . . . . . . . . . 30
3.2.1 Three-dimensionalreconstructionandvisualization 30
3.2.2 Dataanalysis . . . . . . . . . . . . . . . . . . . . 38
4 Discussion 61
4.1 Diffusiontensorimaging . . . . . . . . . . . . . . . . . . 61
4.2 Multi-photonmicroscopy . . . . . . . . . . . . . . . . . . 63
Bibliography 73
List of Figures
1Introduction 1
1.1 Depictionofonehalfoftheleftventricle. . . . . . . . . . 2
1.2 Illustrationofthefibersandthelaminarstructureintheheart. 4
1.3 Schematicdiagramoftheleftventricle. . . . . . . . . . . 5
2MaterialsandMethods 7
2.1 Depictionofthefiberstructureoftheleftventriclefollowing
DTI. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2 SchematicJablonskidiagramofSHG. . . . . . . . . . . . 13
2.3 SchematicdiagramofanSHGmicroscope. . . . . . . . . 15
2.4 Depictionofthefibervs. cross-fiberdirectionofabiaxial
sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.5 Leftventriclewalldividedintoequalslices. . . . . . . . . 18
2.6 Formalinfixedbiaxialsamplebeforeandafterdehydration
andclearing. . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.7 Imaging-setupoftheconfocalmicroscope. . . . . . . . . . 20
2.8 Z-stackdefinitions. . . . . . . . . . . . . . . . . . . . . . 21
2.9 SHGimageofheartmusclefibers. . . . . . . . . . . . . . 22
2.10 Depictionofanintensityplot. . . . . . . . . . . . . . . . 24
2.11 DepictionofthevonMisesdistribution. . . . . . . . . . . 26
3Results 29
3.1 VisualizationofaporcineheartsamplefollowingDTI. . . 29
3.2 Volume-renderedheartmusclefiberstack. . . . . . . . . . 31
X ListofFigures
3.3 Volume rendering of optical sections obtained via SHG
imaging. . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
3.4 Openedupz-stack. . . . . . . . . . . . . . . . . . . . . . 33
3.5 Depictionofvolume-renderedopticalsectionsaquiredby
SHGimaging. . . . . . . . . . . . . . . . . . . . . . . . . 34
3.6 Volume rendering of optical sections obtained by SHG
imaging. . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
3.7 Illustrationoftwodifferentheartsamples. . . . . . . . . . 37
3.8 Intensityplotofthe3-Dmyocytesfiberorientations. . . . 42
3.9 Locationparameterμ,concentrationparameterb,coefficient
ofdeterminationR2,andthedispersionparameterκplotted
overdepth. . . . . . . . . . . . . . . . . . . . . . . . . . 43
3.10 Intensityplot. . . . . . . . . . . . . . . . . . . . . . . . . 44
3.11 Locationparameterμ,concentrationparameterb,coefficient
ofdeterminationR2,andthedispersionparameterκplotted
overdepth. . . . . . . . . . . . . . . . . . . . . . . . . . 45
3.12 Intensityplot. . . . . . . . . . . . . . . . . . . . . . . . . 46
3.13 Locationparameterμ,concentrationparameterb,coefficient
ofdeterminationR2,andthedispersionparameterκplotted
overdepth. . . . . . . . . . . . . . . . . . . . . . . . . . 47
3.14 Intensityplot. . . . . . . . . . . . . . . . . . . . . . . . . 48
3.15 Locationparameterμ,concentrationparameterb,coefficient
ofdeterminationR2,andthedispersionparameterκplotted
overdepth. . . . . . . . . . . . . . . . . . . . . . . . . . 49
3.16 Intensityplot. . . . . . . . . . . . . . . . . . . . . . . . . 51
3.17 Locationparameterμ,concentrationparameterb,coefficient
ofdeterminationR2,andthedispersionparameterκplotted
overdepth. . . . . . . . . . . . . . . . . . . . . . . . . . 52
3.18 Intensityplot. . . . . . . . . . . . . . . . . . . . . . . . . 54
3.19 Locationparameterμ,concentrationparameterb,coefficient
ofdeterminationR2,andthedispersionparameterκplotted
overdepth. . . . . . . . . . . . . . . . . . . . . . . . . . 55
3.20 Representationofintensityplotandparameters. . . . . . . 58
3.21 Illustrationofdepositsembeddedinthecollagenfibers. . . 59
ListofFigures XI
4Discussion 61
4.1 Distinctionbetweenarecentlyclearedsampleandasample
thatstayedintheclearingagentBABBformorethanfive
months. . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
4.2 Examplesofdeficientfieldofviews. . . . . . . . . . . . . 65
4.3 SHG images showing fat deposits embedded in the heart
musclefibers. . . . . . . . . . . . . . . . . . . . . . . . . 66
4.4 SHGimagedetectedinforwarddirection. . . . . . . . . . 67
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