23 INDIVIDUAL TESTS *S16005 ACETYLCHOLINE RECEPTOR ANTIBODY T435 ACTH

64 Pages·2008·1.7 MB·English

INDIVIDUAL TESTS
*Indicates send out test
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NDIVIDUALTESTS
CODE TEST DESCRIPTION
*S16005 ACETYLCHOLINE RECEPTOR ANTIBODY
Specimen: 1mlserum, preferred sample

Method: Immunoprecipitation RIA
Schedule: 7-14 working days
Indication: Diagnosis of acquired Myasthenia gravis.
Interpretive
Guidelines: Apositive titerconfirms a diagnosis ofMyasthenia gravis. Negativetiterscanoccur in 10 to 20%
ofcases, therefore, a negative result does notexclude Myasthenia gravis.
T435 ACTH ( Endogenous level )
Specimen: Plasmafrom LT containing aprotinin.
Method: RIA
Schedule: 7working days
Indication: Todifferentiate pituitary-dependentfrom adrenaltumor hyperadrenocorticism.

Interpretive
Guidelines: Endogenous ACTH concentrations will be increased ( > 45 pg/ml ) in approximately 90%
of dogs with PDH and will be decreased ( < 15 pg/ml ) in approximately 60% of dogs with
adrenal tumor hyperadrenocorticism. A value between 15 and 45 pg/ml is non-diagnostic.
Comments: UseLTcontainingaprotinin for sample collection, centrifuge immediately and transfer plasma
to a plastic vial. ( Antech will provide the necessary tubes. ) Transport on ice or cold packs.
Do not use any plain, uncoated glass tubes or pipettes. Please allow for a minimum of three
daystoobtain the special containerfrom oursupply department.
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CODE TEST DESCRIPTION
T440 ACTH RESPONSE TEST ( CORTISOL 2 )
Specimen: 1 ml serum or heparinized plasma per tube, label tubes PRE and POST
Method: RIA
Schedule: Daily ( M-Sat )
Indication: Investigation of hypoadrenocorticism or hyperadrenocorticism. Monitoring response to
Lysodren or Trilostane treatment.
Special Drawing instructions:
Canine(using Acthar ACTH gel ):
1) Collect pre-sample; label tube "Pre-ACTH."
2) Inject 2.2 IU/kg ( maximum 40 units ) ACTH gel IM.
3) Collect 2 hr post-sample; label "2 hr Post ACTH."

Canine(using synthetic ACTH ):
1) Collect pre-sample; label tube "Pre-ACTH."
2) Inject 0.25 mg Cosyntropin ( Cortrosyn ) IM or IV OR inject 5 ug/kg IV only.
3) Collect 1 hr post-sample; label "1 hr Post ACTH."

Feline ( using Acthar ACTH gel ):
1) Collect pre-sample; label tube "Pre-ACTH."
2) Inject 2.2 IU/kg ACTH gel IM.
3) Collect 1hr and 2 hr post-samples; label accordingly.
Feline ( using synthetic ACTH ):
1) Collect pre-sample; label tube "Pre-ACTH."
2) Inject 0.125 mg Cosyntropin ( Cortrosyn ) IM or IV.
3) Collect 1 hr post sample; label accordingly.
Equine:
1) Draw a resting cortisol. Centrifuge tube, remove the serum and refrigerate.
2) Inject ACTHgel: 0.5I.U. per pound givenIMorsyntheticACTHgelmay be used 100
I.U. given IV or IM.
3) Collect a 4 hr post sample if using the ACTH gel.
Collect a 2 hr post sample if cosyntropin is given IV.
Collect a 2 & 4 hr post sample if cosyntropin is given IM.
Centrifuge the tube, remove the serum and refrigerate.
Comments: If animal is on Prednisone, wait 24 hours before doing test. If animal has received Methyl
Prednisone acetate, wait 6 weeks before drawing. Dexamethasone will not interfere with
cortisol assay, other than suppressing the pre value.
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NDIVIDUAL TESTS
CODE TEST DESCRIPTION
T010 ALBUMIN

Specimen: 1 ml serum, heparinized or EDTA plasma
Method: Bromcresol Green dye
Schedule: Daily
Indication: To assess the quantity of this major protein which maintains colloid osmotic pressure and
acts as a carrier protein for many other compounds.
Interpretive
Guidelines: Decreased with renal or intestinal disease ( increased loss ), or hepatic disease ( decreased
production ). Increase seen only with hemoconcentration due to dehydration.
Comments: 1) Hypoalbuminemia may result in edema/ascites.
2) Hypoalbuminemia may result in a decrease of any compound carried by albumin ( e.g.
Calcium )
T215 ALK PHOS ISOENZYME ( Canine only )
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Specimen: 1 ml serum
Method: Heat inactivation or Levamisole inhibition
Schedule: 1 - 2 working days
Indication: Measures corticosteriod induced fraction of alkaline phosphatase.
Interpretive
Guidelines: Increased with corticosteroids, Cushing’s or persistent stress.
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ODE EST ESCRIPTION
T020 ALKALINE PHOSPHATASE
Specimen: 1 ml serum or heparinized plasma
Method: Kinetic/Bowers&McComb
Schedule: Daily
Indication: Evaluation of hepatic disease ( esp. the biliary system ), hyperadrenocorticism, and less
commonly, bone disorders.
Interpretive
Guidelines: Increased with cholestasis, any condition resulting in increased osteoblastic activity ( e.g.
fracture repair, bone neoplasms ), phenobarbital or phenytoin medication, and
corticosteroid excess.
Comments: 1) Young animals will often show an increase secondary to normal bone growth.
2) In canines ONLY, there is a steroid-induced fraction ( Cushing’s, steroid therapy ),
which can be differentiated from the hepatic fraction by special testing ( heat
resistance test, levamisole inhibition test ).
T030 ALT ( SGPT )
Specimen: 1 ml serum or heparinized plasma
Method: Kinetic/Wroblewski & LaDue
Schedule: Daily
Indication: Evaluation of hepatic disease.
Interpretive
Guidelines: Increase in this cytosolic enzyme indicates cell membrane damage and leakage, secondary
hepatic infection, inflammation, trauma, neoplasia, anoxia/hypoxia, and hepatoxic
compounds ( including a variety of drugs ).
Comments: 1) Value does not necessarily correlate with the degree of hepatic insufficiency.
2) A moderate increase may be induced by glucocorticoids or anti-convulsant medication.
3) Severe muscle damage may cause ALT to increase.
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CODE TEST DESCRIPTION
T040 AMYLASE

Specimen: 1 ml serum or heparinized plasma
Method: Kinetic/CNPG3 substrate
Schedule: Daily
Indication: Evaluation of pancreatic disease.

Interpretive
Guidelines: Increases are seen with pancreatitis, azotemia, and, less commonly, intestinal
disease and hepatic disease.
Comments: 1) Value may not correlate with the severity of pancreatic disease.
2) Amylase is cleared through the kidney; therefore, the value is affected by the
glomerular filtration rate.

T050 AMYLASE and LIPASE
Specimen: 1 ml serum or heparinized plasma
Method: Please refer to individual tests
Schedule: Daily
Indication: Pancreatic disease panel.
*S16872 ANAPLASMA PHAGOCYTOPHILUM TITER ( Canine and Equine only )
Formally called Ehrlichia equi
Specimen: 1 ml serum
Method: IFA
Schedule: 2 - 4 working days
Indication: Investigation of granulocytic ehrlichiosis.
Interpretive
Guidelines: May infect various species; positive results indicate previous or current infection.
Increasing titers seen in recent infections.
Comments: This organism was formally called Ehrlichia equi.
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*S16265 ANAPLASMA PLATYS TITER
Formally called Ehrlichia platys
Specimen: 1 ml serum
Method: IFA
Schedule: 5 - 7 working days
Indication: Check for exposure to A. platys in cases of cyclic thrombocytopenia.
Interpretive
Guidelines: Positive results indicate exposure to A. platys.
Comments: This organism was formally called Ehrlichia platys.
T515 ANTINUCLEAR ANTIBODIES ( ANA )
Specimen: 1 ml serum
Method: IFA
Schedule: 1 - 2 working days
Indication: Evaluation for Systemic Lupus Erythematosus ( SLE ) in patients with appropriate history,
clinical and laboratory findings.
Interpretive
Guidelines: The ANA titer is a sensitive but not specific test for SLE. Elevated ANA titers can occur
in other inflammatory diseases, although these titers tend to be low.
Comments: False negative results can occur from long-term glucocorticoid administration.
*S16055 ARSENIC
Specimen: 1 ml heparinized plasma or LT
1 ml urine or
0.5-1 gram kidney or liver tissue or
10 grams stomach content, feed or water
SHIP ON ICE
Method: Atomic absorption
Schedule: 7 - 10 working days
Indication: Suspicion of arsenic toxicity.
Interpretive
Guidelines: Levels above baseline are consistent with Arsenic toxicity.
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T060 AST ( SGOT )
Specimen: 1 ml serum or heparinized plasma
Method: Kinetic/Enzymatic
Schedule: Daily
Indication: Evaluation of hepatic and muscle disease.
Interpretive
Guidelines: Increase in this enzyme indicates hepatic cell damage ( infection, inflammation, neoplasia,
trauma, anoxia/hypoxia, hepatotoxic compounds ), or muscle disease ( inflammation,
infection, necrosis, trauma, anoxia/hypoxia, neoplasia ).
Comments: 1) Hemolysis may interfere with result.
2) AST is a mitochondrial enzyme, therefore, an increase reflects more serious cell
damage.
3) AST is not as liver-specific as ALT; significant increases in both enzymes suggest the
AST increase is of hepatic origin.
*S16070 BABESIA CANIS TITER
Specimen: 1 ml serum or plasma
Method: IFA
Schedule: 5 - 7 working days
Indication: Detection of occult ( no visible parasitemia ) Babesia canis infections.
Interpretive
Guidelines: A positive titer indicates exposure to, and likely chronic infection with, Babesia canis.
Dogs may be seropositive with no clinical signs of illness.
*S16075 BABESIA GIBSONI TITER ( non export )

Specimen: 1 ml serum or plasma
Method: IFA
Schedule: 7 – 10 working days
Indication: Detection of occult ( no visible parasitemia ) Babesia gibsoni infections.
Interpretive
Guidelines: A positive titer indicates exposure to, and likely chronic infection with, Babesia gibsoni.
Dogs may be seropositive with no clinical signs of illness.
Comments: When exporting use test code *S16502.
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CODE TEST DESCRIPTION
T785 BAERMANN FECAL
Specimen: Feces
Method: Baermann
Schedule: 2 - 7 working days
Indication: Concentration technique used to collect certain parasites in larval stages or as ova; most
often used to detect larvae of respiratory nematodes.
*S16001 BARTONELLA CULTURE ( Cat Scratch Fever )
Specimen: 2 ml LT. Send cold.
Method: Culture
Schedule: 4 weeks
Indication: Investigates cats as carriers of Bartonella.
Interpretive
Guidelines: A positive culture result confirms that the cat is infected with Bartonella. The organism is
present in the blood in low numbers so false negative results may occur. Therefore, a
negative culture result does not exclude the cat as a carrier of Bartonella.
Comments: Bartonella PCR testing and Bartonella serology are more sensitive tests for detecting
carrier cats.
*S85889 BARTONELLA HENSELAE TITER ( Feline only )
Specimen: 1 ml serum
Method: ELISA
Schedule: 5 - 12 working days
Indication: Investigate cats as carriers of Bartonella.
Comments: Additional testing for Bartonella include PCR testing and culture.
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*S1315 BARTONELLA PCR ( Cat Scratch Fever )
Specimen: 1 ml LT, Send cold.
Method: PCR
Schedule: 5 – 7 working days
Indication: Investigate cats as carriers of Bartonella.
Interpretive
Guidelines: A positive test indicates active infection. False negative results may occur due to the low
numbers of circulating organisms but PCR testing is more sensitive than culture.
Comments: Bartonella serology can also be used to investigate Bartonella infection.
T220 BILE ACIDS, PRE AND POST
Specimen: 1 ml serum or heparinized plasma ( pre and post )
Method: Enzymatic
Schedule: 1 - 2 working days
Indication: Tests for hepatic insufficiency or portosystemic shunts.
Interpretive
Guidelines: 1) Elevated levels are seen with decreased hepatic function.
2) Steroid hepatopathies may cause mild to moderate elevations.

3) High levels may be seen with portosystemic shunts.
Comments: Fast for 12 hours prior to drawing pre- sample. Feed a small fatty meal and draw post 2
hours later. Label tubes accordingly.

Occasionally, fasting levels are greater than the post-prandial level. This occurrence may
be due to spontaneous gall bladder contraction prior to feeding.
T225 BILE ACIDS, RESTING
Specimen: 1 ml serum or heparinized plasma
Method: Enzymatic
Schedule: 1 - 2 working days
Indication: May be useful for portosystemic shunts, but pre/post test is recommended.
Interpretive
Guidelines: 1) Elevated levels are seen with decreased hepatic function.
2) Steroid hepatopathies may cause mild to moderate elevations.

3) High levels may be seen with portosystemic shunts.
Comments: Fast for 12 hours prior to draw.

Occasionally, fasting levels are greater than the post-prandial level. This occurrence may
be due to spontaneous gall bladder contraction prior to feeding.
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T070 BILIRUBIN, direct
Specimen: 1 ml serum or heparinized plasma
Method: Van den Bergh & Mueller
Schedule: Daily
Indication: Evaluation of liver and biliary diseases.
Interpretive
Guidelines: Increased levels are seen in biliary disease ( including both intrahepatic and extrahepatic
lesions ) hepatocellular disease ( hepatitis, cirrhosis and advanced neoplastic states )
and hemolytic disease.

T090 BILIRUBIN, total

Specimen: 1 ml serum or heparinized plasma or LT
Method: Colorimetric
Schedule: Daily

Indication: Liver disorders, post hepatic disease, hemolytic disorders.
Interpretive
Guidelines: Elevation may be due to pre-hepatic ( hemolytic ), intrahepatic ( inflammation, infection,
or neoplasia ) or post-hepatic ( adhesion, swelling, neoplasia, etc.) causes.

T520 BLADDER TUMOR antigen (Canine only)
Specimen: 2 ml Urine in RT ( spin urine, submit supernatant )
Method: Latex agglutination
Schedule: 1 - 2 working days
Indication: As a screening test to investigate possible transitional cell carcinoma.
Interpretive
Guidelines: If the Bladder Tumor antigen ( BTA ) is negative, then transitional cell carcinoma is
unlikely ( 90% sensitivity ). Significant pyuria or hematuria, however, may cause false
positives ( 78% specificity ). Animals with positive results should be further evaluated
( ultrasound, radiographs, cytology, biopsy, etc. ) to confirm the presence of neoplasia.

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