2019 A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity_ Applications for coronavirus spike protein

7 Pages·2019·0.32 MB·English

Journal of Visualized Experiments
www.jove.com

Copyright © 2018 Journal of Visualized Experiments
December 2018 | | e58892 | Page 1 of 7

Video Article
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity:
Applications for coronavirus spike protein activation

Javier A. Jaimes*1,2, Jean K. Millet*1,3, Monty E. Goldstein1,4, Gary R. Whittaker1, Marco R. Straus1

1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University
2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University
3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA
4Department of Cell Biology and Molecular Genetics, University of Maryland

*These authors contributed equally

Correspondence to: Gary R. Whittaker at