Accepted Manuscript Title: Development and application of a recombinant M protein-based indirect ELISA for the detection of porcine deltacoronavirus IgG antibodies Authors: Shang-xing Luo, Jing-Hui Fan, Tanja Opriessnig, Jing-Mei Di, Bao-jing Liu, Yu-Zhu Zuo PII: S0166-0934(17)30320-8 DOI: http://dx.doi.org/10.1016/j.jviromet.2017.08.020 Reference: VIRMET 13322 To appear in: Journal of Virological Methods Received date: 19-5-2017 Revised date: 25-8-2017 Accepted date: 26-8-2017 Please cite this article as: Luo, Shang-xing, Fan, Jing-Hui, Opriessnig, Tanja, Di, Jing-Mei, Liu, Bao-jing, Zuo, Yu-Zhu, Development and application of a recombinant M protein-based indirect ELISA for the detection of porcine deltacoronavirus IgG antibodies.Journal of Virological Methods http://dx.doi.org/10.1016/j.jviromet.2017.08.020 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 Development and application of a recombinant M protein-based indirect ELISA for the detection of porcine deltacoronavirus IgG antibodies Shang-xing Luoa,1, Jing-Hui Fana,1, Tanja Opriessnigb, Jing-Mei Dia, Bao-jing Liua, Yu-Zhu Zuoa,c,* aCollege of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, People’s Republic of China. bThe Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK. cCollege of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, People’s Republic of China. 1These authors contributed equally to this work. *Author for all correspondence: Yu-Zhu Zuo Tel.: + 86-312-7528581; fax: + 86-312-7520275 E-mail address: