2016 Maize Genetics Abstract Book

315 Pages·2016·1.98 MB·English

th
58 Annual Maize Genetics Conference
Program and Abstracts

March 17 – March 20, 2016

Hyatt Regency
Jacksonville, Florida
This conference received financial support from:

National Science Foundation
DuPont Pioneer
Syngenta
Monsanto
KenFeng Seed Co.
KWS SAAT AG
National Corn Growers Association
BASF Plant Science
Bayer Crop Science
Dow AgroSciences
Benson Hill
NRGene
AgReliant Genetics
Rapid Genomics

We thank these contributors for their generosity!
Table of Contents

Cover Page ................................................................................................................... i
Contributors ................................................................................................................. ii
Table of Contents ......................................................................................................... iii
General Information ..................................................................................................... iv
Useful Links ................................................................................................................ v
MaGNET Awards ......................................................................................................... vi

Program ........................................................................................................................ 1
List of Posters .............................................................................................................. 7

Abstracts:
Plenary Addresses ................................................................................................... 25
McClintock Awardee............................................................................................... 29
.
Short Talks .............................................................................................................. 30
Posters ..................................................................................................................... 62
Author Index ........................................................................................................... 269

Participants .................................................................................................................... 283

Cover image description

Shelling of variegated kernels at the green house

Cover art by

Stefania Vendramin Alegre
Florida State University
General Information

Meeting Registration
Thursday: 3:00 PM to 9:30 PM: Registration Office (2nd Floor Sky Bridge near Grand ballroom)

Friday: 7 :00AM to 10:00 AM: Registration Office (2nd Floor Sky Bridge near Grand ballroom)

Meals
All meals will be served buffet style in the Grand Ballroom 1-4; serving hours as listed in the Program. Coffee, tea, and
soft drinks are available at no charge during the beverage breaks.

Talks and Posters
All Talks will be presented in the Grand Ballroom 5-8.

Posters will be presented in the Ballroom Foyer, adjacent to the Grand Ballroom, and on the 3rd floor in the St. Johns
and Clearwater rooms. Posters should be hung Thursday starting at 3 PM and stay up until Sunday morning, but must
be removed by 9 AM on Sunday. During poster sessions, presenters of odd number posters are asked to stand by their
posters 1:30-3:00 PM on Friday and 3:00-4:30 PM on Saturday. Presenters of even numbered posters should stand by
their posters 3:00-4:30 PM on Friday and 1:30-3:00 PM on Saturday.

The maize meeting is a forum for presentation and discussion of unpublished material. Photographing or recording of
talks and posters is not allowed.

Hospitality
After the evening sessions on Thursday and Friday there will be informal socializing and poster gazing in the Grand
Ballroom 1-4, with refreshments provided until 1 AM. On Saturday evening there will be informal socializing in the
Grand Ballroom 1-4, with music, dancing and refreshments until 2 AM.

After 1 AM, Suite 4104, adjacent to the 4th Floor Sky Bridge is available for continued socializing. This is a “private
room” for socializing and professional networking, and it is permissible for alcoholic beverages to be brought in;
however, you must stay in this room if you are carrying drinks, and please dispose of all trash and bottles in the room.

Steering Committee
Please share your suggestions and comments about the meeting with the 2016 Steering Committee
David Braun, Chair ........... ([email protected]) Ex officio:
Erich Grotewold, co-Chair.([email protected]) Carson Andorf, abstract coordinator
Mark Settles, Local Host ... ([email protected]) Paula McSteen, Treasurer
Wes Bruce ........................ ([email protected]) Marty Sachs
Alain Charcosset .............. ([email protected]) Mary Schaeffer, abstract coordinator
Sherry Flint-Garcia ........... ([email protected])
Karen McGinnis ............... ([email protected])
Gernot Presting ................. ([email protected])
Ruairidh Sawers ................ ([email protected])
Petra Wolters ..................... ([email protected])
Amanda Wright ................. ([email protected])
Jianbing Yan .................... ([email protected])

Acknowledgements
Many thanks go to John Portwood, Carson Andorf, and Mary Schaeffer for their tremendous efforts in organizing,
assembling, and advertising the conference program. We also greatly thank Angela Freemyer and her team at the
University of Missouri Conference Office for helping to organize the conference, handling registration and dealing with
a multitude of other issues. Special thanks are also extended to Susan Wiley and the Hyatt Regency staff for their help
in organizing this conference, and to Darwin Campbell and John Portwood for providing AV and other support.
Thanks go to Wes Bruce, Petra Wolters, Mark Settles, and David Braun for their efforts in securing funding to support
graduate student attendance at this meeting. Finally, many, many thanks go to Mark Settles for his work as local
organizer and to Marty Sachs for his wisdom in all things related to the Maize Meeting.
iv
Useful Links

2016 Maize Meeting Website

http://maizegdb.org/maize_meeting/2016

2017 Maize Meeting Website (Available November 2016)

http://maizegdb.org/maize_meeting/2017

Abstract Book (Electronic version)

http://maizegdb.org/maize_meeting/abstracts/2016Program.pdf

Cover Image

http://maizegdb.org/maize_meeting/coverart/

v
The MaGNET Program and 2016 Awards
MaGNET (Maize Genetics Network Enhancement via Travel) is a program that seeks to recruit
and retain scientists from diverse backgrounds into the maize research community by encouraging their
attendance at the Annual Maize Genetics Conference (MGC). As such, it provides a source of support to
help students and early career scientists from under-represented groups learn about maize genetics and
connect with scientists already in the community. Awardees are not required to have previous maize
genetics research experience, but will hopefully develop an appreciation of the current excitement in the
field, and become an integral part of the community in the future. The program also provides an
opportunity for awardees to explore potential collaborations and develop career contacts.
Each MaGNET Award helps defray the cost of attending the Maize Genetics Conference, including
registration, food, lodging and airfare. In addition, awardees that have never attended the MGC are
paired with an experienced ‘Maize Mentor’, who will help the awardee navigate the conference.
Awardees are identifiable by a special notation on their name tags, and many of them are attending the
MGC for the first time – please congratulate these scientists and welcome them to our famously
hospitable conference!
All applicants must show strong potential for a career in the biological sciences, be either citizens or
permanent residents of the USA, and belong to a group traditionally underrepresented in science. To
help provide a more integrative and effective experience at the Conference for student awardees, faculty
mentors who accompany one or more eligible student applicants are also eligible to apply for a
MaGNET award.
2016 MaGNET Awardees
Undergraduate
Mahliyah Adkins-Threats, Truman State University Poster #159
Cairo Archer, Boyce Thompson Institute for Plant Research Poster #135
Suzi Barboza-Pacheco, Oklahoma State University
Merritt Burch, University of Hawaii at Hilo
Asha Cotterell, Spelman College Poster #376
Stephanie Diaz, University of South Carolina – Aiken Poster #353
Morgan James, Langston University
Weschester Junior, Florida A&M University
Sydney Lyda, Florida A&M University Poster #198
Umnia Mahgoub, Iowa State University Poster #117

Graduate Student
Oliver Bear Don’t Walk IV, Stanford University Poster #219
Israel Jimenez Luna, California State University - Los Angeles
Muriel Longstaff, Brigham Young University Poster #218

Scientist
Roselyn Hatch, Purdue University Poster #146

Faculty Mentor Accompanying Student
Gokhan Hacisalihoglu, Florida A&M University Poster #65
Ramesh Katam, Florida A&M University Poster #198

The MaGNET program of the Maize Genetics Conference is supported by grant IOS-
1608773 from the National Science Foundation, Plant Genome Research Program.
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Schedule of Events

Talks will be held in the Grand Ballroom 5-8.
Posters will be displayed in the Ballroom Foyer (adjacent to Grand Ballroom)
and on the 3rd floor in the St. Johns and Clearwater rooms.

Thursday, March 17

2:00 PM – 6:00 PM OPTIONAL PRE-CONFERENCE WORKSHOPS

2:00 PM – 3:30 PM MaizeGDB problem solving workshop (Daytona Room)
3:30 PM – 4:30 PM Gramene tutorial (Daytona Room)
4:30 PM – 6:00 PM Zea mays genome assemblies (Daytona Room)

Pre-registration recommended for the above sessions.

3:00 PM – 8:30 PM REGISTRATION (2nd Floor Sky Bridge near Grand Ballroom)

3:00 PM – 6:00 PM POSTER HANGING (Ballroom Foyer and 3rd floor in St. Johns and
Clearwater Rooms)

6:00 PM – 7:00 PM DINNER (Grand Ballroom 1-4)

7:00 PM – 9:00 PM SESSION 1 – PLENARY TALKS
Chair: David Braun Pages 26 & 27

7:00 PM WELCOME AND ANNOUNCEMENTS (Grand Ballroom 5-8)
David Braun

7:15 PM Karen Koch, University of Florida [Plen 1]
Sugar in your ears: Regulating transport, metabolism, and
consequences

8:05 PM Jonathan Lynch, Pennsylvania State University [Plen 2]
Phenes and genes for improved water and nutrient capture in maize

9:00 PM – 10:00 PM REGISTRATION (2nd Floor Sky Bridge near Grand Ballroom)

9:00 PM – 1:00 AM INFORMAL POSTER VIEWING & HOSPITALITY
(Grand Ballroom 1-4)
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Friday, March 18

7:00 AM – 8:00 AM BREAKFAST (Grand Ballroom 1-4)
7:00 AM – 8:15 AM REGISTRATION (2nd Floor Sky Bridge near Grand Ballroom)

8:00 AM – 10:15 AM SESSION 2 - COMPUTATIONAL & LARGE-SCALE BIOLOGY
Chair: Alain Charcosset Talks 1-6. Pages 31-36.

8:00 AM ANNOUNCEMENTS (Grand Ballroom 5-8)
David Braun

8:15 AM Amanda Waters, University of Minnesota [T1]
Natural variation and cis-regulatory variation for response to abiotic
stress in maize

8:35 AM Julia Bailey-Serres, University of California, Riverside [T2]
Ancient sensing and response networks regulate flooding tolerance –
So what’s up with maize?

8:55 AM Ana Paula Alonso, The Ohio State University [T3]
Deciphering kernel metabolism: how metabolomics and fluxomic can
guide us

9:15 AM Bo Wang, Cold Spring Harbor Laboratory [T4]
Unveiling the complexity of maize transcriptome by single-molecule
long-read sequencing

9:35 AM Linda Hanley-Bowdoin, North Carolina State University [T5]
DNA replication timing programs during development of maize root tips

9:55 AM Johann Joets, Institute for Agricultural Research (INRA) [T6]
Genome-wide sequence analysis of the maize FV2 inbred line provides
new insights into molecular and chromosomal features of
presence/absence variants and their implication in European maize
genetic originality

10:15 AM BREAK

10:45 AM – 12:25 PM SESSION 3 – NEW TOOLS & APPROACHES FOR MAIZE
Chair: Karen McGinnis Talks 7-11. Pages 37-39

10:45 AM Johanna Smyth, Oregon State University [T7]
Proteomic profiling suggests control of translation and protein
stability is crucial for pollen tube germination in maize (Zea mays)

11:05 AM Maike Stam, University of Amsterdam [T8]
Identification and characterization of distant enhancers in Zea mays

11:25 AM Sergei Svitashev, DuPont Pioneer [T9]
Maize genome editing using CRISPR/Cas9 technology

11:45 AM Elizabeth Lee, University of Guelph [T10]
Genomes to Fields’ Maize GxE Project: Expression of productivity and
phenological traits across a diverse set of environments

12:05 PM Addie Thompson, Purdue University [T11]
Beyond GWAS: Characterizing drought response of elite temperate
and tropical maize using a systems-biology approach
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Friday, March 18 (continued)

12:30 PM – 1:30 PM LUNCH (Grand Ballroom 1-4)

1:30 PM – 5:00 PM POSTER SESSION 1 (Ballroom Foyer and 3rd floor in St. Johns and
Clearwater Rooms)

1:30 PM – 3:00 PM Presenters should be at odd numbered posters.
3:00 PM – 4:30 PM Presenters should be at even numbered posters.

Beverages will be available from 3:30 PM to 5:00 PM.

4:40 PM – 6:00 PM SESSION 4 – GENOME STRUCTURE & CYTOGENETICS
Chair: Ruairidh Sawers Talks 12-15. Pages 40-43.

4:40 PM Zhikai Liang, University of Nebraska-Lincoln [T12]
B73 maize population structure analysis by RNA-seq data

5:00 PM Michelle Stitzer, University of California, Davis [T13]
Ecological determinants of transposable element distributions in the
B73 maize genome

5:20 PM Gernot Presting, University of Hawaii [T14]
Resolving the centromere paradox – microevolution of centromeric
DNA repeats

5:40 PM Arnaud Ronceret, Universidad Nacional Autónoma de México[T15]
Analysis of maize meiotic mutants to fix heterosis

6:00 PM – 7:00 PM DINNER (Grand Ballroom 1-4)

7:00 PM – 9:00 PM SESSION 5 – MCCLINTOCK PRIZE PRESENTATION
Chair: Sherry Flint-Garcia McClintock Talk. Page 30

7:00 PM Nathan Springer, MGEC Chair
Early- and Mid-Career Awards

Ron Phillips, University of Minnesota
McClintock Prize Presentation

7:40 PM Jeffrey D. Palmer, Indiana University at Bloomington [M1]
Horizontal gene transfer – the dark matter of plant genomes and plant
evolution

9:00 PM – 1:00 AM INFORMAL POSTER VIEWING & HOSPITALITY
(Grand Ballroom 1-4)
3
Saturday, March 19

7:00 AM – 8:00 AM BREAKFAST (Grand Ballroom 5-8)

8:00 AM – 10:20 AM SESSION 6 – QUANTITATIVE GENETICS & BREEDING
Chair: Jianbing Yan Talks 16-22. Pages 44-50.

8:00 AM Jorge Alberto Romero Navarro, Cornell University [T16]
Identifying the diamond in the rough: Studying allelic variation for
complex traits in maize landraces

8:20 AM Tingting Guo, Iowa State University [T17]
Optimal design of genomic prediction in maize hybrid breeding

8:40 AM Jason Wallace, The University of Georgia [T18]
The effect of host genetics on the maize leaf microbiome across 270
diverse inbred lines

9:00 AM Xiaohong Yang, China Agricultural University [T19]
Genome-wide association study reveals genetic basis and role of
ZmVPP1 in drought tolerance in maize seedlings

9:20 AM Sebastien Praud, Biogemma [T20]
BALANCE, a powerful population to decipher complex traits in maize

9:40 AM Lei Liu, Huazhong Agricultural University [T21]
KRN4 controls quantitative variation in maize kernel row number

10:00 AM Shuhua Zhan, University of Guelph [T22]
The genetic basis and adaptive significance of transcript abundance
differences between two maize inbreds

10:20 AM – 10:45 AM BREAK

10:45 AM – 12:25 PM SESSION 7 – CELL & DEVELOPMENTAL GENETICS
Chair: Amanda Wright Talks 23-27. Pages 51-55.

10:45 AM Karina van der Linde, Stanford University [T23]
Biomolecular interpretation of the Trojan horse myth: Use of Ustilago
maydis to analyze the function of maize (Zea mays) MAC1

11:05 AM Pablo Martinez, University of California Riverside [T24]
TANGLED-1 function in maize

11:25 AM Kin Lau, Purdue University [T25]
Induced and natural variation in genes encoding the microtubule
severing ATPase, katanin p60 (KTN1), alter meristem shape, plant
morphology and spikelet density

11:45 AM Marisa Rosa, University of California at Berkeley [T26]
The maize Mid-Completing Activity protein NARROW ODD DWARF
is required for normal plant growth and leaf pattering

12:05 PM Josh Strable, Iowa State University [T27]
Maize YABBY genes drooping leaf1 and drooping leaf2 affect
agronomic traits by regulating leaf and floral architectures

4