2015 Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification PDF
Preview 2015 Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification
Accepted Manuscript Title: Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification Author: Manabu Nemoto Yoshinori Morita Hidekazu Niwa Hiroshi Bannai Koji Tsujimura Takashi Yamanaka Takashi Kondo PII: S0166-0934(15)00023-3 DOI: http://dx.doi.org/doi:10.1016/j.jviromet.2015.02.001 Reference: VIRMET 12715 To appear in: Journal of Virological Methods Received date: 5-9-2014 Revised date: 3-2-2015 Accepted date: 4-2-2015 Please cite this article as: Nemoto, M., Morita, Y., Niwa, H., Bannai, H., Tsujimura, K., Yamanaka, T., Kondo, T.,Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification, Journal of Virological Methods (2015), http://dx.doi.org/10.1016/j.jviromet.2015.02.001 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 21 Accepted Manuscript 1 Highlights 1 An RT-LAMP assay was developed for detection of equine coronavirus 2 The RT-LAMP assay was more sensitive than conventional RT-PCR 3 Quantitative RT-PCR was more sensitive than RT-LAMP 4 RT-LAMP allows for rapid and simple detection of equine coronavirus 5 6 Page 2 of 21 Accepted Manuscript 2 Short communication 6 7 Rapid detection of equine coronavirus by reverse transcription loop-mediated 8 isothermal amplification 9 10 Manabu Nemotoa,*, Yoshinori Moritab, Hidekazu Niwaa, Hiroshi Bannaia, Koji 11 Tsujimuraa, Takashi Yamanakaa, Takashi Kondoa 12 13 aEpizootic Research Center, Equine Research Institute, Japan Racing Association, 14 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan 15 bTokachi Draft Horse Clinic, Nishi 13, Minami 9-1, Obihiro, Hokkaido 080-0023, Japan 16 17 E-mail address of each author: 18 M. Nemoto:
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