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2011 The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent PDF

1 Pages·2011·0.03 MB·English
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Preview 2011 The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent

CORRIGENDUM The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent Aartjan J. W. te Velthuis, Jamie J. Arnold, Craig E. Cameron, Sjoerd H. E. van den Worm and Eric J. Snijder Nucl. Acids Res. 2010; 38, 203–214. doi: 10.1093/nar/gkp904 The authors wish to apologise for an error in the concentration of tetracycline used to induce SARS-coronavirus nsp12 expression in E. coli. In the second paragraph of Materials and Methods, the sentence: ‘‘Subsequently, the cells were slowly cooled to room temperature, tetracycline was added to a final concentration of 200 mg/ml and the cells were further grown at 20�C for another 16 h.’’ should read ‘‘Subsequently, the cells were slowly cooled to room temperature, tetra- cycline was added to a final concentration of 200 ng/ml and the cells were further grown at 20�C for another 16 h.’’ 9458 Nucleic Acids Research, 2011, Vol. 39, No. 21 doi:10.1093/nar/gkr963 � The Author(s) 2011. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. at University of Memphis - Library on April 14, 2015 http://nar.oxfordjournals.org/ Downloaded from

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