Theriogenology EL SEV IER Theriogenology 59 (2003) 2029-2040 Contents of Theriogenology Volume 59 VOLUME 59 ISSUE 1 1 JANUARY 2003 Announcement Fundamental Aspects Gene expression and chromatin structure in the pre-implantation embryo J. Kanka (Libechov, Czech Republic) Mammalian epigenomics: reprogramming the genome for development and therapy W. Reik, F. Santos and W. Dean (Cambridge, UK) Nuclear reprogramming of cloned embryos produced in vitro Y.M. Han, Y.K. Kang, D.B. Koo and K.K. Lee (Daejeon, South Korea) Assisted Reproductive Technologies I Coordination between donor cell type and cell cycle stage improves nuclear cloning effi- ciency in cattle D.N. Wells, G. Laible, F.C. Tucker, A.L. Miller, J.E. Oliver, T. Xiang, J.T. Forsyth, M.C Berg, K. Cockrem (Hamilton, New Zealand), PJ. L’ Huillier (Armadale, Australia), H.R Tervit and B. Oback (Hamilton, New Zealand) Bringing up small oocytes to eggs in pigs and cows lr. Miyano (Kobe, Japan) Assisted Reproductive Technologies II Prospects for spermatogenesis in vitro J.E. Parks (Ithaca, NY, USA), D.R. Lee (Seoul, South Korea), S. Huang and M.T. Kaproth (Ithaca, NY, USA) — New microinsemination techniques for+ leben itory » anlenals A. Ogura, N. Ogonuki, K. Inoue and K. Mochida (Ibaraki, Japan) . Transgenic Technology Development of efficient strategies for the production of genetically modified pigs H. Nagashima (Kawasaki, Japan), T. Fujimura, Y. Takahagi (Tsukuba, Japan), M Kurome, N. Wako, T. Ochiai, R. Esaki (Kawasaki, Japan), K. Kano (Fujisawa, Japan), S. Saito (Yaita, Japan), M. Okabe (Suita, Japan) and H. Murakami (Tsukuba, Japan) Artificial chromosome vectors and expression of complex proteins in transgenic animals J.M. Robl, P. Kasinathan, E. Sullivan (Sioux Falls, SD, USA), Y. Kuroiwa, K. Tomizuka and I. Ishida (Gunma, Japan) . Pll: S0093-691X(03)00052-9 Transgenic swine for biomedicine and agriculture R.S. Prather (Columbia, MO, USA), R.J. Hawley (Charlestown, MA, USA), D.B. Carter, L. Lai (Columbia, MO, USA) and J.L. Greenstein (Charlestown, MA, USA) Commercialization Commercialization of D.C. Faber, J.A olin hirichs Vander Zwaag and L.B. Ferre (Sioux Center IA, USA) New commercial opportunities for advanced reproductive technologi n horses, wildlife and companion animals C.R. Lon S.C. Walker, R.T. Tang and M.E. Westhusin (Colle la n, TX, USA) Current Status of Embryo Technologies in horses, sheep and deer technologies in the horse E.M. Carnevale, P.M. McCue and J.E. Bruemmer (1 tatus of embryo technologies in sheep and goat nie, G. Baril, N. Poulin and P. Mermillod (Nouzilly, Fr: velopments reproductive technologies in deer D.K. Berg (Hamilton, New Zealand) and G.W. Asher (Mos ABSTRACTS FOR POSTER PRESENTATION Student Competition Finalists Ab tr VOLUME 59 ISSUE 2 15 JANUARY 2003 Pretace Pasture-based dairying: challenges and rewards for New Zez G. Verkerk (Hamilton, New Zealand) Dairy cattle production in Europe J.A.M. van Arendonk (Wageningen, The Netherlands) a1 Liinamo (Oosterbeek, The Netherlands) Artificial insemination: the state of the art R. Vishwanath (Hamilton, New Zealand) Economics of selecting for sex: the most important genetic trait G.E. Seidel Jr. (Fort Collins, CO, USA) Bovine embryo technologies C. Galli, R. Duchi, G. Crotti, P. Turini, N. Ponderato, S. Colleoni, 1. Lagutina and G Lazzari (Cremona, Italy) Factors affecting commercial application of embryo technologies in New Zealand: a modelling approach D.C. Smeaton, B.L. Harris, Z.Z. Xu (Hamilton, New Zealand) and W.H. Vivanco (Echuca, Australia) Factors affecting commercial application of embryo technologies in dairy cattle in Europe a modelling approach J.A.M. van Arendonk and P. Bijma (Wageningen, The Netherlands) Contents of Volume S59 Factors affecting oocyte quality and quantity in commercial application of embryo technol ogies in the cattle breeding industry 1.S. Merton, A.P.W. de Roos, E. Mullaart, L. de Ruigh, L. Kaal (Arnhem, The Nether lands), P.L.A.M. Vos and S.J. Dieleman (Utrecht, The Netherlands) In vitro production of bovine embryos and their application for embryo transfe1 H. Hoshi (Yamagata, Japan) Improving successful pregnancies after embryo transfer \.J. Peterson and R.S-F. Lee (Hamilton, New Zealand) VOLUME 59 ISSUES 3-4 FEBRUARY 2003 Improved collection and developmental competence of immature macaque oocytes C.A. VandeVoort (Davis, CA, USA), S.P. Leibo (New Orleans, LA, USA) and A.I farantal (Davis, CA, USA) Buffalo and cattle hybrid embryo development is decreased by caffeine treatment during in vitro fertilization B.G. Tatham (Werribee, Australia), T. Feehan (Rutherglen, Australia) and R. Pashen (Clayton, Australia) Stage-specific effects of the osmolarity of a culture medium on the development of parthe nogenetic diploids in the pig V.T. Nguyen, S. Kure-bayashi, H. Harayama (Kobe City, Japan), T. Nagai (Ibaraki, Japan) and M. Miyake (Kobe City, Japan) Effects of dead spermatozoa on motion characteristics and membrane integrity of live spermatozoa in fresh and cooled-stored equine semen S.P. Brinsko, T.L. Blanchard, S.L. Rigby, C.C. Love and D.D. Varner (College Station, PX, USA) Cryopreservation of canine semen using a coconut water extender with egg yolk and three different glycerol concentrations R. de Cassia Soares Cardoso, A.R. Silva, D.C. Uchoa and L.D.M. da Silva (Fortaleza, Brazil) Reproductive period affects lipid composition and quality of fresh and stored spermatozoa in Turkeys V. Douard (Nouzilly, France), D. Hermier (Orsay, France), M. Magistrini and E. Blesbois (Nouzilly, France) Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes L.B. Love, Y.H. Choi, C.C. Love, D.D. Varner and K. Hinrichs (College Station, TX, USA) Effects of pregnant mare serum gonadotropin (eCG) on follicle development and granulosa- cell apoptosis in the pig Z.H. Liu, K.Z. Yue, S.F. Ma, X.S. Sun (Harbin, PR China) and J.H. Tan (Taian City, PR China). ee a re ae Lymphocyte-mediated lysis of sheep chorion: susceptibility of chorionic cells to third-party and maternal cytotoxic lymphocytes and presence of cells in the endometrium exhib.ting cytotoxicity toward natural-killer cell targets S. Tekin (Gainesville, FL, USA and Tokat, Turkey) and P.J. Hansen (Gainesville, FL, USA) Contents of Volume S59 Effects of body condition score and score change on the reproductive performance of dairy cows: a meta-analysis F. Lopez-Gatius (Lleida, Spain), J. Yaniz (Zaragoza, Spain) and D. Madriles-Helm (Lleida, Spain) Recovery and cryopreservation of sika deer (Cervus nippon) spermatozoa from epididy- mides stored at 4 C M. Hishinuma (Tottori, Japan), K. Suzuki (Tokyo, Japan) and J. Sekine (Tottori, Japan) Quality of canine semen submitted to single or fractionated glycerol addition during the freezing process A.R. Silva, R.C.S. Cardoso, D.C. Uchoa and L.D.M. Siiva (Ceara, Brazil) Production of transgenic goats by pronuclear microinjection of in vitro produced zygotes derived from oocytes recovered by laparoscopy H. Baldassarre, B. Wang, N. Kafidi, M. Gauthier, N. Neveu, J. Lapointe, L. Sneek, M Leduc, F. Duguay, J.F. Zhou, A. Lazaris and C.N. Karatzas (St. Telesphore, Canada) Male-specific DNA sequences in pigs Y.-M. Horng (Chiayi, Taiwan and Taichung, Taiwan) and M.-C. Huang (Taichung, Taiwan) Effects of different sexual stimuli on oxytocin release, uterine activity and receptive beha- vior in estrous sows P. Langendijk, E.G. Bouwman (Wageningen, The Netherlands), D. Schams (Weihenste- phan, Germany), N.M. Soede and B. Kemp (Wageningen, The Netherlands) Effects of thioredoxin on the preimplantation development of bovine embryos Y.Z. Bing, Y. Hirao, N. Takenouchi, L.M. Che (Morioka, Japan), H. Nakamura, J. Yodoi (Kyoto, Japan) and T. Nagai (Ibaraki, Japan) Cryopreservation of semen from endangered pheasants: the first step towards a comdvasli for endangered avian species M. Saint Jalme, R. Lecog (Cleres, France), F. Seigneurin, E. Blesbois (Nouzilly, France) and E. Plouzeau (Cleres, France) Use of two replacements of serum during bovine embryo culture in vitro P. Duque, E.G omez, E. Diaz, N. Facal, C. Hidalgo and C. Diez (Asturias, Spain) 8389 Activity of angiotensin-converting enzyme (ACE) in reproductive tissues of the stallion and effects of angiotensin II on sperm motility B.A. Ball, C.G. Gravance, M.T. Wessel and K. Sabeur (Davis, CA, USA) Components of litter size in gilts with different prolactin receptor genotypes B.T.T.M. van Rens (Wageningen, The Netherlands), G.J. Evans (Cambridge, UK) and r. van der Lende (Wageningen, The Netherlands) rhe association between lameness, ovarian cysts and fertility in lactating dairy cows P. Melendez, J. Bartolome, L.F. Archbald, A. Donovan (Gainesville, USA) Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development A.A. Ali, J.F. Bilodeau and M.A. Sirard (Quebec, Canada) Comparison of two protocols for the treatment of retained fetal membranes in dairy cattle M. Drillich, A. Pfiitzner, H.-J. Sabin, M. Sabin and W. Heuwieser (Berlin, Germany) Production of piglets using intracytoplasmic sperm injection (ICSI) with flowcytometrically sorted boar semen and artificially activated oocytes S. Probst and D. Rath (Neustadt, Germany). Effects of oviductal and cumulus cells on in vitro fertilization and embryo dovelogueant of porcine oocytes fertilized with epididymal spermatozoa R. Romar, P. Coy, S. Ruiz, J. Gadea (Murcia, Spain) and D. Rath (Mariensee, Germany) Contents of Volume 59 In vitro maturation of bovine cumulus—oocyte complexes in undiluted follicular fluid: effect on nuclear maturation, pronucleus formation and embryo development B. Avery, L. Strgbech, T. Jacobsen, 1.B. Bégh and T. Greve (Frederiksberg C, Denmark) Pregnancy rates in mares following hysteroscopic or transrectally-guided insemination with low sperm numbers at the utero-tubal papilla S.P. Brinsko, S.L. Rigby (College Station, TX, USA), A.C. Lindsey (Fort Collins, CO, USA), T.L. Blanchard, C.C. Love and D.D. Varner (College Station, TX, USA) 1001 Purification and characterization of a protein tyrosine acid phosphatase from boar seminal vesicle glands P. Wysocki and J. Strzezek (Olsztyn, Poland) Behavioural development in the neonatal lamb: effect of maternal and birth-related factors C.M. Dwyer (Edinburgh, UK) Studies of the pathogenesis of bovine pestivirus- induced ovarian dysfunction in superovu- lated dairy cattle M.R. McGowan (St. Lucia, Australia), M. Kafi (Shiraz, Iran), P.D. Kirkland (Menangle, Australia), R. Kelly, H. Bielefeldt-Ohmann (St. Lucia, Australia), M.D. Occhio (Rockhampton, Australia) and D. Jillella (St. Lucia, Australia) Health and Safety Advisory Committee (HASAC) of the International Embryo Transfe1 Society (IETS) has managed critical challenges for two decades M. Thibier (Rome, Italy) and D.A. Stringfellow (Auburn, AL, USA) 1067 Announcements ; 1079 Guide for Authors ; , : 1081 VOLUME 59 ISSUES 5-6 MARCH 2003 Repeatability and accuracy of reproductive tract scoring to determine pubertal status in beef heifers K.S. Rosenkrans and D.K. Hardin (Columbia, MO, USA) . . In vitro survival of bovine spermatozoa stored at room temperature under epididymal con- ditions I.M.C. De Pauw, A. Van Soom (Merelbeke, Belgium), K. Mintiens (Brussels, Belgium), S. Verberckmoes and A. de Kruif (Merelbeke, Belgium) Effect of sperm coating on the survival and penetrating ability of in vitro stored bovine spermatozoa I.M.C. De Pauw, A. Van Soom, D. Maes, S. Verberckmoes and A. de Kruif (Merelbeke, Belgium) ; Bovine and buffalo in vitro embryo sodaction using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration G. Neglia, B. Gasparrini, V.C. di Brienza, R. Di Palo, G.C ampanile (Napoli, Italy), G.A. Presicce (Rome, Italy) and L. Zicarelli (Napoli, Italy) Pregnancy-associated glycoprotein concentrations during pregnancy mad the postpartum period in Azawak Zebu cattle N.M. de Sousa (Santa Maria, Brazil), M. Zongo, W. Pitala, H. Boly, L. Sawadogo, M Sanon (Burkina Faso, Brazil), J.R. de Figueiredo, P.B.D. Gongalves (Santa Maria, Brazil), B. El Amiri, Z. Perenyi and J.-F. Beckers (Liege, Belgium) Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa G.-P. Li, G.E. Seidel Jr. and E.L. Squires (Fort Collins, CO, USA) 2034 Contents of Volume 59 Fertility of ram semen frozen in Bioexcell“ and used for cervical artificial insemination J. Gil (Uppsala, Sweden and Paysandi, Uruguay), M. Rodriguez-Irazoqui (Paysandu, Uruguay), N. Lundeheim, L. Séderquist and H. Rodriguez-Martinez (Uppsala, Sweden) Changes in the distribution of lectin receptors during capacitation and acrosome reaction in boar spermatozoa I. Jimenez, H. Gonzalez-Marquez, R. Ortiz, J.A. Herrera, A. Garcia, M. Betancourt and R. Fierro (México, Mexico) Association of uterine edema with follicle waves around the onset of the breeding season in pony mares E.D. Watson (Midlothian, UK), R. Thomassen (Midlothian, UK and Oslo, Norway) and E. Nikolakopoulos (Midlothian, UK and Athens, Greece) Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low- grade buffalo semen ejaculates Z. Ahmad, M. Anzar, M. Shahab, N. Ahmad and S.M.H. Andrabi (Islamabad, Pakistan) 1189 Estrus induction following PGF2~ treatment in the superovulated buffalo (Bubalus bubalis) A.K. Misra and H.C. Pant (Kaira, India) 1203 New technology for vitrification and field (microscope-free) warming and transfer of small ruminant embryos V. Isachenko (Zaragoza, Spain and Cologne, Germany), J.L. Alabart (Zaragoza, Spain), M. Dattena (Olmedo-Sassari, Italy), F.N awroth (Cologne, Germany ), P. Cappai (Olmedo- Sassari, Italy), E. Isachenko (Cologne, Germany), M.J. Cocero (Madrid, Spain), J Olivera, A. Roche (Zaragoza, Spain), C. Accardo (Olmedo-Sassari, Italy), A. Krivo- kharchenko (Berlin, Germany) and J. Folch (Zaragoza, Spain) Effects of gas conditions, time of medium change, and ratioo f medium to embryo on in vitro development of horse oocytes fertilized by intracytoplasmic sperm injection Y.-H. Choi, C.C. Love, D.D. Varner, L.B. Love and K. Hinrichs (College Station, TX, USA) Development of bovine oocytes reconstructed with a nucleus from growing stage oocytes after fertilization in vitro S. Bao (Tokyo, Japan), H. Ushijima (Chiba, Japan), A. Hirose, F. Aono (Ibaraki, Japan), Y. Ono and T. Kono (Tokyo, Japan) Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen J. Gil, N. Lundeheim, L. S6derquist and H. Rodriguez-Martinez (Uppsala, Sweden) Serum-free culture of murine primordial germ cells and embryonic germ cells lr. Horii, Y. Nagao (Kyoto, Japan), T. Tokunaga (Tsukuba, Japan) and H. Imai (Kyoto Japan) Factors affecting the survival of sheep embryos after transfer within a MOET program F. Bari (Ceredigion, UK), M. Khalid (Hertfordshire, UK), W. Haresign (Ceredigion, UK) \. Murray and B. Merrell (Northumberland, UK) Studies of the cell cycle of in vitro cultured skin fibroblasts in goats: work in progress Y.S. Yu, X.S. Sun, H.N. Jiang, Y. Han, C.B. Zhao (Harbin, PR China) and J.H. Tan (Shandong Province, PR China) Double radial immunodiffusion as a tool to identify pregnancy-associated glycoproteins in ruminant and nonruminant placentae B. El Amiri (Sart Tilman, Belgium and Meknes, Morocco), N. Melo de Sousa, K. Mecif, H. Desbuleux, H. Banga-Mboko and J.-F. Beckers (Sart Tilman, Belgium) Reproduction in female yaks (Bos grunniens) and opportunities for improvement X.-D. Zi (Sichuan, PR China) Contents of Volume 59 Impact of consuming tall fescue seeds infected with the endophytic fungus, Neotyphodium coenophialum, on reproduction of chickens M.R. Conover (Logan, UT, USA). Effect of probe location on changes in vaginal electrical ienpeds ince dusing the porcine estrous cycle P. Rezaé, M. Péschl and I. Kfivanek (Brno, Czech Republic) Effect of local interaction of reactive oxygen species with prostaglandin F,, on the release of progesterone in ovine corpora lutea in vivo K. Hayashi, A. Miyamoto, A. Konari, M. Ohtani and Y. Fukui (Obihiro, Japan) Screening of some variables influencing the results of embryo transfer in the ewe. Part Il Two-day-old embryos J.L. Alabart, J. Folch, A. Fernandez-Arias, J.P. Ramon, J.M. Garbayo (Zaragoza, Spain) and M.J. Cocero (Madrid, Spain) ; lhe effect of intrauterine administration of estradiol on postpartum uterine involution in cattle I.M. Sheldon, D.E. Noakes, A.N. Rycroft (Herts, UK) and H. Dobson (Neston, UK) The effect of vitamins during maturation of caprine oocytes on subsequent developmental potential in vitro C.L. Bormann, E.M. Ongeri and R.L. Krisher (West Lafayette, IN, USA) Secretion patterns of oxytocin and PGF2%-metabolite in response to cervical dilatation in cyclic mares J. Handler, M. KOnigshofer (Vienna, Austria), H. Kindahl (Uppsala, Sweden), D. Schams (Freising-Weihenstephan, Germany) and C. Aurich (Vienna, Austria) Growth factors and growth hormone enhance in vitro embryo production and post-thaw survival of vitrified bovine blastocysts N.R. Mtango (Yamaguchi, Japan), M.D. Varisanga (Dar es Salaam, Tanzania), YJ. Dong, R. Rajamahendran and T. Suzuki (Yamaguchi, Japan) Serum thyroid hormones and reproductive characteristics of Rambouillet ewe lambs treated with propylthiouracil before puberty N.H. Wells, D.M. Hallford and J.A. Hernandez (Las Cruces, NM, USA) Use of primers derived from a new sequence of the bovine Y chromosome for sexing Bos taurus and Bos indicus embryos B.C.A. Alves (Sao Paulo, Brazil), V.F.M. Hossepian de Lima (Jaboticabal, Brazil), C.M feixeira and C.A. Moreira-Filho (Sao Paulo, Brazil) Apoptosis within bovine follicular cells and its effect on oocyte development during in vitro maturation \. Zeuner (Berlin, Germany and Schénow, Germany), K. Miiller, K. Reguszynski (Schonow, Germany) and K. Jewgenow (Berlin, Germany) Efficacy of linear and convex transducers for ultrasound-guided transvaginal follicle aspiration M.M. Seneda (Londrina, Brazil), C.R. Esper, J.M. Garcia (Jaboticabal, Brazil), E.R. Andrade (Londrina, Brazil), M. Binelli (Pirassununga, Brazil), J.A. Oliveira (Jaboticabal, Brazil) and \.B. Nascimento (Londrina, Brazil) Selective control of the estrous cycle of the dog through suppression of estrus and reduction of the length of anestrus r.C. Phillips, R.E. Larsen, J. Hernandez, L. Strachan, D. Samuelson, V.M. Shille and L.I Archbald (Gainesville, FL, USA) Effects of epidermal growth factor on early embryonic development after in vitro fertiliza tion of oocytes collected from ewes treated with follicle stimulating hormone A.T. Grazul-Bilska, J.T. Choi, J.J. Bilski, R.M. Weigl, J.D. Kirsch, K.C. Kraft, L.P. Reynolds and D.A. Redmer (Fargo, ND, USA) 2036 Contents of Volume 59 Ovarian response, embryo production and hormonal profile in superovulated goats treated with insulin S. Selvaraju (Bangalore, India), S.K. Agarwal (Izatnagar, India), S.D. Karche (Uttar Pradesh, India) and A.C. Majumdar (Izatnagar, India) Rabbit and pig ear skin sample cryobanking: effects of storage time and temperature of the whole ear extirpated immediately after death M.A. Silvestre, A.M. Saeed, R.P. Cervera, M.J. Escriba and F. Garcia-Ximénez (Valencia, Spain) 1469 Short communication Exogenous prostaglandin F,% at time of ovulation improves reproductive efficiency in repeal breeder sows G. Horvat and G. Bilkei (Diibendorf, Switzerland) Announcement VOLUME 59 ISSUE 7 1 APRIL Seasonal timing of sperm production in roe deer: interrelationship among changes in ejaculate parameters, morphology and function of testis and accessory glands F. Goeritz, M. Quest, A. Wagener, M. Fassbender, A. Broich, T.B. Hildebrandt, R.R Hofmann and S. Blottner (Berlin, Germany) Gonadotropin exposure, salt storage and storage duration affect penetration of domestic cat oocytes by homologous spermatozoa J.R. Herrick and W.F. Swanson (Cincinnati, OH, USA) Presence of caprine arthritis—encephalitis virus (CAEV) proviral DNA in genital tract tissues of superovulated dairy goat does F. Fieni, J. Rowe, K. Van Hoosear, C. Burucoa, S$. Oppenheim, G. Anderson, J. Murray and R. BonDurant (Davis, CA, USA) Efficacy of the anticaspase agent zVAD-fmk on post-thaw viability of canine spermatozoa \.T. Peter (West Lafayette, IN, USA) and C. Linde-Forsberg (Uppsala, Sweden) Endocrine events during the periestrous period and the subsequent estrous cycle in ewes after estrus synchronization J. Menegatos, S. Chadio, T. Kalogiannis (Athens, Greece), T. Kouskoura and S. Kouimtzis (Thessaloniki, Greece) Effects of methanol and developmental arrest on chilling injury in zebrafish (Danio rerio) embryos lr. Zhang, X.-H. Liu and D.M. Rawson (Bedfordshire, UK) Normal telomere lengths of spermatozoa in somatic cell-cloned bulls N. Miyashita (Ibaraki, Japan), K. Shiga, T. Fujita, H. Umeki, W. Sato (Oita, Japan), 1 Suzuki (Iwate, Japan) and T. Nagai (Ibaraki, Japan) Timing of nuclear maturation of nonstored and stored domestic cat oocytes L. Katska-Ksiazkiewicz, B. Rynska, G. Kania, Z. Smorag, B. Gajda (Knoxville, TN, USA and Balice/Cracow, Poland) and M. Pienkowski (Knoxville, TN, USA) Effect of reducing sperm concentration during IVF on the ability to distinguish between bulls of high and low field fertility: work in progress F. Ward, D. Rizos, M.P. Boland and P. Lonergan (County Dublin, Ireland) Contents of Volume 59 Influence of oxygen tension on apoptosis and hatching in bovine embryos cultured in vitro Y.Q. Yuan, A. Van Soom, F.0O.J. Coopman, K. Mintiens (Merelbeke, Belgium), M.L. Boerjan (Zeddam, The Netherlands), A. Van Zeveren, A. de Kruif and L.J. Peelman (Merelbeke, Belgium) \ sporadic case of the sex-reversed mare (64,XY ; SRY-negative): molecular and cytogenetic studies of the Y chromosome M. Bugno (Balice, Poland), J. Klukowska (Poznan, Poland), E. Stota (Balice, Poland), M. Tischner (Krakow, Poland) and M. Switonski (Poznan, Poland) Birth of piglets after deep intrauterine insemination with flow cytometrically sorted boat spermatozoa J.M. Vazquez, E.A. Martinez, |. Parrilla, J. Roca, M.A. Gil (Murcia, Spain) and J.L Vazquez (Elche, Spain) 1605 In vitro and in vivo comparison of Ham’s F-10, Emcare holding solution and ViGro holding plus for the cooled storage of equine embryos M. Moussa (Nouzilly, France and Nantes, France), G. Duchamp (Nouzilly, France), R. Mahla (Jouy-en-Josas, France), J.-F. Bruyas (Nantes, France) and P.F. Daels (Nouzilly, France) Evaluation of fresh and frozen-thawed semen of individual ganders by assessment of spermatozoa motility and morphology E. Lukaszewicz and W. Kruszynski (Wroclaw, Poland) Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos S.H. Hyun, G.S. Lee, D.Y. Kim, H.S. Kim, S.H. Lee, S. Kim (Seoul, South Korea), E.S. Lee (Chunchon, South Korea), J.M. Lim (Suwon, South Korea), S.K. Kang, B.C. Lee (Seoul, South Korea) and W.S. Hwang (Seoul, South Korea and Suwon, South Korea) Lack of association of Mycobacterium avium subsp. paratuberculosis with oocytes and embryos from moderate shedders of the pathogen r.A.M. Kruip (Lelystad, The Netherlands), J. Muskens (Deventer, The Netherlands), H.J.W. van Roermund, D. Bakker and N. Stockhofe-Zurwieden (Lelystad, The Nether lands) Metabolic profiles and progesterone cycles in first lactation dairy cows V.J. Taylor (Hertfordshire, UK), D.E. Beever, M.J. Bryant (Berkshire, UK) and D.C Wathes (Hertfordshire, UK) 1661 2002 Reviewer Appreciation 1679 Announcements 1683 VOLUME 59 ISSUE 8 15 APRIL 2003 Excess nuclear DNA in spermatozoa of guinea fowl J. Barna (Godollo, Hungary) and G.J. Wishart (Dundee, UK) 1685 Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation D.M. Neild (Capital Federal, Argentina), B.M. Gadella (Utrecht, The Netherlands), M.G Chaves, M.H. Miragaya (Capital Federal, Argentina), B. Colenbrander (Utrecht, The Netherlands) and A. Agiiero (Capital Federal, Argentina) 1693 2038 Contents of Volume 59 Postpartum reproductive function: association with energy, metabolic and endocrine status in high yielding dairy cows M Reist (Zurich, Switzerland and Berne, Switzerland), D.K. Erdin, D. von Euw, K.M Ischiimperlin, H. Leuenberger (Zurich, Switzerland), H.M. Hammon, C Morel, C. Phi- lipona, Y. Zbinden (Berne, Switzerland), N. Kiinzi (Zurich, Switzerland) and J.W. Blum (Berne, Switzerland) Effects of Equex from different sources on post-thaw survival, longevity and intracellular Ca™ concentration of dog spermatozoa \.1. Pefia, L.L. Lugilde, M. Barrio, P.G. Herradon and L.A. Quintela (Lugo, Spain) FSH priming improves oocyte maturation, but priming with FSH or hCG has no effect on subsequent embryonic development in an in vitro maturation program S.M. Junk, A. Dharmarajan and J.L. Yovich (Perth, Australia) Effect of leukemia inhibitory factor on bovine embryos produced in vitro under chemically defined conditions S. Sirisathien, H.J. Hernandez-Fonseca, P. Bosch, B.R. Hollet, J.D. I ott and B.G. Brackett (Athens. GA, USA) Effect of processing and measuring procedures on estimated sizes of bull sperm heads R.H. Foote (Ithaca, NY, USA) Somatic cell nuclear transfer in domestic cat oocytes treated with IGF-I for in vitro maturation Y. Kitiyanant (Bangkok, Thailand and Nakorn Pathom, Thailand), J. Saikhun (Nakorm Pathom, Thailand) and K. Pavasuthipaisit (Bangkok, Thailand and Nakorn Pathom, Thailand) Use of medroxyprogesterone acetate (MAP) in lactating Holstein cow s within an Ovsynch protocol: follicular growth and hormonal patterns D. Cavestany (Colonia, Uruguay), A. Meikle (Montevideo, | ruguay), H. Kindahl ({ ppsala Sweden), Van Lier (Montevideo, Uruguay), F. Moreira, W.W. Thatcher (Gainesville, FL, USA) and M. Forsberg (Uppsala, Sweden) The effects of GnRH administration postinsemination on serum concentrations of proges terone and pregnancy rates in dairy cattle exposed to mild summer heat stress S. Willard, S. Gandy, S. Bowers, K. Graves (Mississippi State, MS, USA), A. Elias and C. Whisnant (Raleigh, NC, USA) Calculated follicle deviation using segmented regression for modeling diameter differences in cattle D.R. Bergfelt (Madison, WI, USA and Cross Plains, WI, USA), L.H. Sego (Madison, WI, USA), M.A. Beg and O.J. Ginther (Madison, WI, USA and Cross Plains, WI, USA) Fixed-time insemination in peripuberal, lightweight replacement beef heifers after estrus synchronization with PGF2alpha and GnRH C.R. Dahlen (St. Paul, MN, USA) G.C. Lamb (Grand Rapids, MN, USA), C.M. Zehnder L.R. Miller and A. DiCostanz(oS t. Paul, MN, USA) Cryopreservation of goat oocytes and in vivo derived 2- to 4-cell embryos using the cryoloop (CLV) and solid-surface vitrification (SSV) methods I. Begin, B. Bhatia, H. Baldassarre (Vaudreuil-Dorion, Canada), A. Dinnyes (Godollo, Hungary) and C.L. Keefer (Vaudreuil-Dorion, Canada) Vitrification of rat embryos at various developmental stages M.-S. Han, K. Niwa (Okayama, Japan) and M. Kasai (Kochi, Japan) Semen characteristics of genetically identical quadruplet bulls C. Lessard, I. Masseau, J.-F. Bilodeau (Sainte-Foy, Canada), T. Kroetsch (Guelph, Canada), H. Twagiramungu (St-Hyacinthe, Canada), J.L. Bailey, P. Leclerc and R. Sulli- van (Sainte-Foy, Canada)