Surface ultrastructure of the egg chorion of Zinaspa youngi Hsu & Johnson (Lepidoptera: Lycaenidae)

2013·11.1 MB·

TThheLee piLdeoppteirdoloopgitcaelrSooclieotygical Society ooff JJaapapnan
ue 2 :wh Lepidopte,'a S('ien( '6e4 (1) :27-29. April 2013
Surface ultrastructure of the egg chorion of Zinaspa youngi Hsu & Johnson (Lepidoptera:
Lycaenidae)
Hai-Ming Xu and Min WANG
Department of Entomotog.y, South ChinaAs,ricultural University. Guangzhou,Guan.gdong 510642, P. R. China
Abstract The structures of Lepidopterou esggshells are usual]y significant for the study of taxonomy. Hcre. we describe
the surface u]trastructure of the eg.o.. sot' Zinaspa .i'cnt wnitghi scanning electron microscopy (SEM) for the fir sttime,
Key wordsZina.v)a }'oitn gui]t.rustructure. ]nicropylar rosette. aeropy]es, SErv{.
Introduction Results and Discussion
The stnictures of insec etgs,shells ure usually quit ecomplex. The egg (Figs 3-10) is rounded in dorsa lview with a
Thesculpturingoftheextemalmorphologyofeggsappears diamete ref 54-56 pm and slightly less in length (Fig 3.}.
to be specific and is considered to be of grea tinterest The shape looks lik ea sea urchin. The micropyle is centered
becauseofpotentialtaxonomicapplications(Rosciszeowns kthae, upper surface and encircled by 4-6 petal-shaped
1991).The cells (Fi g6s-8) .The micropyle area is slightly depressed
sfacLoa,nr n is2nthg0udi0ygi7nh g) el.reecs tordlouintaiogn nmoicsr tosaicnco dpceh a3ra-ctd emriasmkeen osfiit oainnn as ilecnlvcarat ilts{yuVai bonlfee teto thoeetl Taefbnnhoodteirrrm edseauee drrrrsoou peuryb nfdlbydaeec. de pv5e - ono6p fte fnb aiutnygnhg seons enomclaeh- o lrsaairnh oednaro pw fesh duei n axs eonarfaoeg poylasletn-ereasors.pelyhols aeccso pepTl iehlc(sde ;F (i riFeg da ing,cee9hgts wj)soo4 .riTkn-ceh5lel).
Zinaspa is a genus in the famil yLycaenidae T.here are each other and tubercles protrud eupward from the
six species in Zina.s7)a in previou sIiteratur (eWang and juneti ognivsi.n .tohe appearance of fiows from a volcanic
Fang. 2002) .Zinaspa .voitn gwias named by Hsu and eruption (Fig. 9) .CIesc-u pofjunctions is shown in Fig.
Johnson (]998) .Photos of egg, ]arv aand the pupa were 10.Some
seugpgp lciheodri oinn t hofe aZritniacslep ,ab u.ty tohee tsnu grwfaiasce n outlt rreapsotrrtuecdt.ure of the For ex avmaprlieations th ew ernuemb errevea loefd pien dSaElM- sihmaapgee dcs e(llFsi gi n6s -t8he).
Here, sN' edescrib tehe ultrastructure of the eg.os ofZinaspa micrepyie area ranged from 4 to 6. In our research. we
.l'ott nwigtih scanning electron microseopy (SEM). think that this may be individu arlather than geographic
variability. The lary aoi' Zinaspa .vottn giis simi]ar to that
Materials and Methods obfy Stihteii ewrggJsdher lqlastte (n'!egtaorruam sa,nbhdui t Ftuhkeyu dcaa ,n2 0boeo d) iT.shtei tnugbeuricslheed
Adults (Fig s1-2) appcared in late March. Thc eggs were on the egg of Sui'entbz tqtteiz'etort "hnas an obtuse end
laid by the female in the wild in Nanlin.e National Nature and the number of tubercle i sles sthan in Zina,yJa .voitngi.
Reserve. Guangdong province in lat eMarch. Most eggs Thc larv aof Zinaspa .votmgi is also allied to that of
were laid singly on the stem or bud. We collected somc Ambiopatct at,idiena, but their eggs have some obvious
eggs on the host plant and ptaced them in a small culture
dish.Egs,s
were washed thorough]y with distil] weatder using
a soft brush and air dried. AII procedures were performed
at 25CC. Next, the matcrial was sputtered ",ith gold and
examined and photographed under a scanning electren
microscope (XL-30-ESEM )E.gg length and width were
measuredunderSEM.Al}eggsexaminedareoftheupright
type, with the micrepyle on the :"top" surfacc and opposite
the '"bottom" surface, Approximately 8 es,gs were examined. Figs t-2. Fema]e of Zinasptt .yottn g1 /i U.pperside. 2/ Underside.
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TThheLee piLdeoppteirdoloopgitcaelrSooclieotygical Society ooff JJaapapnan
28 H,-M, Xuand M. WANG
Figs 3-10. Egg of Zinaspa .voung 3i/. Dorsal view of whole egg. 4-5: Tbp view ofmicropy]ar area and aerepy!es.
6-8 /Genera vliew of anterior egg pole revealing the rosette of petals. 9/ The later uvliew of the egg
chorion showing the ridges und aeropyles. 1O: Close-up ofjunction.
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TThhee　 LLeepipdiopdteorpoltoegiroaollogical　 SSoooiceityety 　ooff　 JJaapapnan
Surface　ultrastructure 　of　egg 　chorien 　of　Zinaspa　youngi 29
differenc．　eFsor　example ，　the　micropyle 　area 　ofA ηめJqρ（1’α 　 　Entomol，4：68−81．
αりid’8πα is　deeper　than　in　Z’naspa 　youngi　and　the　end　of Wa皿g，　M ，and　X ．　L 　Fan，2002．　Buttediies　Fauna　Sinic：aLycaenida，e
tubercle　is　more 　rounded ． 　　pp．147．　Henan　Science　and 　Tヒchnology 　Publishin　gHouse，
　　Zhengzhou ．
Acknow 置edgments
摘　要
We　thank　Chen　Xinfang　for　expertise 　in　SEM 　in　ilstmment］a
Analysis ResearchCenterinSouthChinaAgricultural Zinaspa　youngi （鱗翅目：シジミチ ョ ウ科）の卵殻の表
＆ 　 　 　 　 　 ・
Universit．y 面微細構造 （徐　海明 王　敏）
Zinaspa　youngiはHsu　and 亅ohnson （199S）によっ て記載され，
Refe『ences 卵殻の・幼表虫面微・蛹細構な造どのを写走真査が型示電子され顕た微鏡だけを用だいっ たて．記今載回し，た卵．
Hsu，YFand 　KJohnson ，1998．A　new 　species 　of　Zinaspa　frorn 卵殻の隆起線は網目状 となっ て多数の小室を形成し，接合
　　　　C5h：i3n9a−4（L2e．pidopter：aLycaenida：eThechnae）．　Holarcti正celガd 部辺での盛卵弁り．数Lがはっ46て火と違山い噴が火見を思られわたせがる，突個起体と変な異ると．精思孔わ周れ
I　ga　rabsuht，it　Ser．　faindi，　ep　slHs．．　3F0u1ku−d3a03．，　T2o0k0ai0　．　UTnhivee　rlsiifty　eh　Pirsetsos，r　　TyoDfk　yAosi，an る．
Roscizewska，　E ，1991．Ultrastructura　land 　histochemic　asltudies 本種の 幼虫は （同 じム ラサキ シ シ ミ族の ）Surendra
　　of 出e　egg　capsule 　of　Perla　marginata （Panzer，1799）and quercetorum やAmblopala　avidiena の 幼虫 に 似 る が．　s．
　　　　DIinnt・o∫crIanss　ececphta”loteosrphol （．　CEu〃r毘tわiり，sり1’8．2270）：（1P8l9e−c2o0p3t．er：aPerlid）a・e qと”e．rcまetoたruAmmbtのop卵alのa　a隆vid起ien線a 結の合卵部のは精よ孔り域鈍はくよ数りが深少くな，い隆起こ
Vineet，　K ．，　B ．　K．　Kariappa，　A．　M ．　Babu　and 　S．B．　Dandin ，2007． 線結合部はよりまるみを帯びる．（文責 ：広渡俊哉）
　　Su血 ce　Ultrastuctu　rofe　the　Egg　Chorion　of　Eri　Silkwom1，
　　Samia　ricini （Donovan ）（Lepidoptera ：Saturniida）e，　J． （Received　January　21，2013．　AccepIed　Febr岨 ry　21，2013）
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