NASA Technical Reports Server (NTRS) 20130009000: Streamline-based microfluidic device

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(12) United States Patent (lo) Patent No.: (cid:9) US 8,354,075 B1
Tai et al. (45) Date of Patent: (cid:9) Jan. 15, 2013
(54) STREAMLINE-BASED MICROFLUIDIC 2002/0041827 Al * (cid:9) 4/2002 Yager et al ...................... 422/57
DEVICE 2003/0159999 Al * (cid:9) 8/2003 Oakey et al . .................. 210/695
2004/0166025 Al * (cid:9) 8/2004 Chan et al ....................... 422/99
(75) Inventors: Yu-Chong Tai, Pasadena, CA (US); OTHER PUBLICATIONS
Siyang Zheng, Pasadena, CA (US);
Ayon, A.A. et al., "Characterization of a Time Multiplexed Induc-
Harvey Kasdan, Sherman Oaks, CA
tively Coupled Plasma Etcher," Journal of the Electrochemical Soci-
(US) ety, 1999, vol. 146, No. 1, pp. 339-349.
Huang, L.R. et al., "Continuous Particle Separation Through Deter-
(73) Assignees: California Institute of Technology, ministic Lateral Displacement," Science, May 14, 2004, vol. 304, pp.
Pasadena, CA (US); IRIS International, 987-990.
Yamada, M. et al., "Pinched Flow Fractionation: Continuous Size
Inc., Chatsworth, CA (US)
Separation of Particles Utilizing a Laminar Flow Profile in a Pinched
Microchannel,"Analytical Chemistry, Sep. 15, 2004, vol. 76, No. 18,
(*) Notice: (cid:9) Subject to any disclaimer, the term of this pp. 5465-5471.
patent is extended or adjusted under 35 Yamada, M. et al., "Hydrodynamic Filtration for On-Chip Particle
U.S.C. 154(b) by 481 days. Concentration and Classification Utilizing Microfluidics," Lab on a
Chip, 2005, vol. 5, pp. 1233-1239.
Zheng, S. et al., "Deterministic Lateral Displacement MEMS Device
(21) Appl. No.: 11/408,514
for Continuous Blood Cell Separation," Eighteenth IEEE Interna-
tional Conference on Micro Electro Mechanical Systems (MEMS
(22) Filed: (cid:9) Apr. 20, 2006 '05): Miami, USA, Jan. 30-Feb. 2, 2005, pp. 851-854.
Related U.S. Application Data * cited by examiner
(60) Provisional application No. 60/673,572, filed on Apr.
Primary Examiner Dean Kwak
21, 2005.
(74) Attorney, Agent, or Firm Kilpatrick Townsend &
Stockton LLP.
(51) Int. Cl.
BOIL 3100 (cid:9) (2006.01)
(57) (cid:9) ABSTRACT
GOIN1/00 (cid:9) (2006.01)
(52) U.S. Cl. ........ 422/503; 422/500; 422/501; 422/502; The present invention provides a streamline-based device and
422/504; 422/505; 436/180 a method for using the device for continuous separation of
(58) Field of Classification Search ............ 422/99-104, particles including cells in biological fluids. The device
422/500-505; 436/180 includes a main microchannel and an array of side micro-
See application file for complete search history. channels disposed on a substrate. The main microchannel has
a plurality of stagnation points with a predetermined geomet-
(56) (cid:9) References Cited ric design, for example, each of the stagnation points has a
predetermined distance from the upstream edge of each of the
U.S. PATENT DOCUMENTS side microchannels. The particles are separated and collected
in the side microchannels.
5,091,328 A (cid:9) 2/1992 Miller
6,592,821 BI* (cid:9) 7/2003 Wada et al . .................. 422/68.1
6,632,619 BI* 10/2003 Harrison et al . ............... 435/7.2 12 Claims, 10 Drawing Sheets
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Jan. 15, 2013 (cid:9) Sheet 1 of 10 (cid:9) US 8,354,075 B1
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