microbial synthesis and characterization of nano scale silver material and evaluation of their bio

202 Pages·2017·13.29 MB·English

“MICROBIAL SYNTHESIS AND
CHARACTERIZATION OF NANO
SCALE SILVER MATERIAL AND
EVALUATION OF THEIR BIO
EFFICACY AGAINST RICE SHEATH
BLIGHT PATHOGEN Rhizoctonia solani
(Kuhn)”

N. CHIRANJEEVI

B.Sc. (Ag.)

MASTER OF SCIENCE IN AGRICULTURE
(PLANT PATHOLOGY)
2015
“MICROBIAL SYNTHESIS AND

CHARACTERIZATION OF NANO SCALE SILVER
MATERIAL AND EVALUATION OF THEIR BIO
EFFICACY AGAINST RICE SHEATH BLIGHT
PATHOGEN Rhizoctonia solani (Kuhn)”

BY
N. CHIRANJEEVI

B.Sc. (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE

(PLANT PATHOLOGY)

CHAIRPERSON: Dr. P. ANIL KUMAR

DEPARTMENT OF PLANT PATHOLOGY
SRI VENKATESWARA AGRICULTURAL COLLEGE, TIRUPATI
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
LAM FARM, GUNTUR – 522 509
2016
DECLARATION

I, N. CHIRANJEEVI, hereby declare that the thesis entitled
“MICROBIAL SYNTHESIS AND CHARACTERIZATION OF NANO
SCALE SILVER MATERIAL AND EVALUATION OF THEIR BIO
EFFICACY AGAINST RICE SHEATH BLIGHT PATHOGEN
Rhizoctonia solani (Kuhn)” submitted to the Acharya N. G. Ranga
Agricultural University for the award of degree of Master of Science in
Agriculture in the major field of PLANT PATHOLOGY is the result of
original research work done by me. I also declare that no material contained
in the thesis has been published earlier in any manner.

Place : (N. CHIRANJEEVI )
I.D. No.: TAM-2014/022
Date :
CERTIFICATE
Mr. N. CHIRANJEEVI has satisfactorily prosecuted the course of
research and that thesis entitled “MICROBIAL SYNTHESIS AND
CHARACTERIZATION OF NANO SCALE SILVER MATERIAL
AND EVALUATION OF THEIR BIO EFFICACY AGAINST RICE
SHEATH BLIGHT PATHOGEN Rhizoctonia solani (Kuhn)” submitted
is the result of original research work and is of sufficiently high standard to
warrant its presentation to the examination. I also certify that neither the
thesis nor its part thereof has been previously submitted by her for a degree
of any University.

Date : (P. ANIL KUMAR)
Chairperson
Professor and Head
Department of Plant Pathology
Agricultural College,
Bapatla - 522 101,
Guntur Dt., A. P.
CERTIFICATE
This is to certify that the thesis entitled “MICROBIAL SYNTHESIS
AND CHARACTERIZATION OF NANO SCALE SILVER
MATERIAL AND EVALUATION OF THEIR BIO EFFICACY
AGAINST RICE SHEATH BLIGHT PATHOGEN Rhizoctonia solani
(Kuhn)” submitted in partial fulfillment of the requirements for the award of
degree of Master of Science in Agriculture to the Acharya N. G. Ranga
Agricultural University, Guntur, is a record of the bonafide research work
carried out by N. CHIRANJEEVI under our guidance and supervision.

No part of the thesis has been submitted by the student for any other
degree or diploma. The published part and all assistance and help received
during the course of the investigations have been duly acknowledged by the
author of the thesis.
Thesis approved by the Student’s Advisory Committee:
Chairperson : Dr. P. ANIL KUMAR ______________
Professor and Head
Dept. of Plant Pathology
Agricultural College, Bapatla-522101
Guntur Dt., A. P.
Member : Dr. R. SARADA JAYALAKSHMI ______________
Professor (Plant Pathology) and
University Librarian
Admn. Camp Office, Vijaya Durga
Towers, MG Inner Ring Road, Guntur
522509, A.P.
Member : Dr. K. V. HARI PRASAD ______________
Assistant Professor
Dept. of Entomology
S. V. Agricultural College,
Tirupati – 517 502, A. P.
Member : Dr. T. N. V. K. V. PRASAD _____________
Senior Scientist
Dept. of Soil Science and Agril.
Chemistry IFT, RARS,
Titupati – 517502, A. P.
Date of final viva-voce:
ACKNOWLEDGEMENT
Acknowledgement is written at last, placed at first and read the least, but still
it is the only opportunity to thank one and all who are responsible in completion of
the thesis in time during the course of study and research work.

Accomplishment of this thesis is the result of benevolence of almighty,
blessings of my parents, motivation of my teachers and help of my friends.

At the very outset, whole heartedly I wish to express my fidelity and deep sense
of gratitude to the chairman of my advisory committee Dr. P. Anil Kumar, Professor,
Department of Plant Pathology, Agricultural College, Tirupati for his inspiring
guidance, timely advice, constant supervision, encouragement affectionate dealing
through the period of investigation during preparation of my manuscript. I feel elated
to express my heartfelt thanks for his immense interest transcendent suggestions,
affectionate encouragement and adroitness in handling the research work in all its
stages.
I am very much pleased to place my appreciation and gratitude to the member
of my advisory committee Dr. R. Sarada jayalakshmi devi, Professor (Plant
Pathology) and University librarian, Guntur for his amiable suggestions, benevolent
and noble ideas and kind cooperation during pursuit of my research work.

I deem it my privilege in expressing my deep sense of reverence and
gratitude and indebtedness to Dr. T.N.V.K.V. Prasad, Senior Scientist,
Department of Soil Science and Chemistry, IFT, RARS, Tirupati and member of
my advisory committee for his talented guidance, valuable suggestions, careful
and reasoned criticism and meticulous attention to the details which has led to the
present investigation to the final shape.

I accord my sincere thanks to Dr. K.V. Hari Prasad, Assistant Professor,
Department of Entomology, S. V. Agricultural College, Tirupati for his valuable
guidance and suggestions during the course of the research work.

I offer my profuse regards and thanks to Dr. B. Bhaskara padmodaya,
Professor and Head, Department of Plant Pathology for her caring encouragement
and indefatigable interest as well as providing equipped laboratory facilities during
my research work.
I cordially offer my profound gratitude to Dr. Khayum Ahammed Scientist
Dept. of Plant Pathology, Dr. M. Reddy kumar, Associate professor, M. K.
Jyothsna, S. Salam and P. Arunasri and Gurivi Reddy Assistant Professors,
Department of Plant Pathology for their constant encouragement, valuable
comments, timely help and cooperation during the course of my study and research
work.
From my mere existence on earth to present situation, my every step of life is
moulded mud by my mother N. Vemakka and father N. Anjaneyulu to whom I will
forever remain indebted. Their parenting brings every time the best in every effort of
journey. My way in this world paves through the path carved out by my parents
whom, I wish to idolize throughout my life. I also acknowledge each and every chunk
of help, guidance and suggestions, from my lovely sisters Marry, Marry, Lora,
Prabha, Sailaja, Vasantha, brothers Suresh, Ravi, Ramesh, and Chinna and well
wishers that contributed for the success of my work. I wish to acknowledge my
beloved, family members Laxman, Jesus, Teja, Mahesh, Raghu and Israel for their
affection and love. I extend my affection to my cousins Kasturi, Swetha, Chinnari,
Dhanush, Jessy, Uma, and charan for their pleasant companionship throughout my
life.

My hearty thanks to my seniors Raghavendra, Ravi, Chaitanya, Thimmappa,
Narasimhulu, Mallikarjuna, Paramesh, Praveen, Bhaskar, Yohan, Venkat,
Rasheed, Raj, Ayyapu, Naveen, C. Mouli, Narasimhulu, sathish, D.V. Naik,
Chandu, Somla naik, Ranganna, Prabhu, Ranga rani, Shaheeda, Sunil, Shankar,
Sunil, Haseena, Madhuri, Vineel, Chandu, Naga Lakshmi, Nazeer, Rakhi,
Raghavendra, Jeevula, Nagarjuna,vinod Bhanu, Rajitha, and Vijay for the much
needed moral support, encouragement, guidance, valuable suggestions and help
during the course of my study.
Words fail in expressing my affection and gratitude towards my best pals
Irfan, Bindu, Nandu, Raji, Johnavi, Madhu, Ravi, Bhavani, Chintu and Konda for
their help and constant encouragement. I remember to point the time of days spent
with my friends Amar, Lucky, Yesdan, Brahmi, Chitti, Uma, Aparna, Sreelatha,
salma, Raza, Naresh, and Hanu for their splendid company.

My heartfelt thank to my junior friends Chandu, Mahendra, Sharath, Raju,
Chari, Ramana, Suresh, Prudvi, Shiva, Hari, Lokesh, Midhya Mahesh, Rama
Naidu, Bhuvan, Thippu, Gomu, Nagesh, and Sai for their friendly assistance,
valuable cooperation and suggestions during my research work.

I was thankful to Acharya N. G. Ranga Agricultural University for giving me
the opportunity to pursue my post graduation successfully, for providing the facilities
to conduct my research work.

I am very much thankful to non-teaching staff, Chenchiah, Changalrayulu,
Ravi and Padma for their help during my PG Programme in the Department.

Finally word of appreciation goes to Sri Sai Xerox (Ramu), Balaji Colony,
Tirupati for printing this thesis in time.
chiranjeevi…
CONTENTS

Chapter No. Title Page No.
I INTRODUCTION
II REVIEW OF LITERATURE
III MATERIAL AND METHODS
IV RESULTS AND DISCUSSION
V SUMMARY AND CONCLUSIONS
LITERATURE CITED